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Yorodumi- PDB-8e6n: X-ray structure of the Deinococcus radiodurans Nramp/MntH divalen... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8e6n | |||||||||
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Title | X-ray structure of the Deinococcus radiodurans Nramp/MntH divalent transition metal transporter G223W mutant in an outward-open, manganese-bound state | |||||||||
Components | Divalent metal cation transporter MntH | |||||||||
Keywords | METAL TRANSPORT / Membrane protein / divalent transition metal importer / LeuT fold / NRAMP family | |||||||||
Function / homology | Function and homology information manganese ion transmembrane transporter activity / cadmium ion transmembrane transporter activity / symporter activity / cellular response to iron ion / metal ion binding / plasma membrane Similarity search - Function | |||||||||
Biological species | Deinococcus radiodurans (radioresistant) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | |||||||||
Authors | Bozzi, A.T. / Ray, S. / Zimanyi, C.M. / Nicoludis, J.M. / Gaudet, R. | |||||||||
Funding support | United States, 1items
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Citation | Journal: Elife / Year: 2023 Title: High-resolution structures with bound Mn 2+ and Cd 2+ map the metal import pathway in an Nramp transporter. Authors: Ray, S. / Berry, S.P. / Wilson, E.A. / Zhang, C.H. / Shekhar, M. / Singharoy, A. / Gaudet, R. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8e6n.cif.gz | 179.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8e6n.ent.gz | 139.3 KB | Display | PDB format |
PDBx/mmJSON format | 8e6n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e6/8e6n ftp://data.pdbj.org/pub/pdb/validation_reports/e6/8e6n | HTTPS FTP |
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-Related structure data
Related structure data | 8e5sC 8e5vC 8e60C 8e6hC 8e6iC 8e6lC 8e6mC 6c3iS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 44480.680 Da / Num. of mol.: 1 / Mutation: G223W Source method: isolated from a genetically manipulated source Source: (gene. exp.) Deinococcus radiodurans (radioresistant) Gene: mntH, DR_1709 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9RTP8 |
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-Non-polymers , 5 types, 80 molecules
#2: Chemical | #3: Chemical | ChemComp-OLC / ( #4: Chemical | ChemComp-PEG / #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.46 Å3/Da / Density % sol: 49.95 % / Description: 40 micron in size |
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Crystal grow | Temperature: 298 K / Method: lipidic cubic phase / pH: 6 Details: 50 mM succinic acid, 10 mM spermidine, 5 mM manganese chloride, 100 mM MES, 28% PEG400 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Apr 15, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→39.19 Å / Num. obs: 13962 / % possible obs: 82.1 % / Redundancy: 3.5 % / Biso Wilson estimate: 58.94 Å2 / CC1/2: 0.98 / Rmerge(I) obs: 0.186 / Rpim(I) all: 0.095 / Rrim(I) all: 0.21 / Net I/σ(I): 6.2 |
Reflection shell | Resolution: 2.4→2.49 Å / Redundancy: 1.7 % / Mean I/σ(I) obs: 1.6 / Num. unique obs: 621 / CC1/2: 0.58 / % possible all: 36.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 6C3I Resolution: 2.4→39.19 Å / SU ML: 0.32 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 34.32 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 208.45 Å2 / Biso mean: 74.9973 Å2 / Biso min: 37.12 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.4→39.19 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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