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- PDB-8ch0: Crystal structure of an 8-repeat consensus TPR superhelix with Ga... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8ch0 | ||||||
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Title | Crystal structure of an 8-repeat consensus TPR superhelix with Gadolinium. | ||||||
![]() | Consensus tetratricopeptide repeat protein | ||||||
![]() | DE NOVO PROTEIN / CTPR / polymorphism / metal coordination | ||||||
Function / homology | GADOLINIUM ATOM![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liutkus, M. / Rojas, A.L. / Cortajarena, A.L. | ||||||
Funding support | European Union, 1items
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![]() | ![]() Title: Diverse crystalline protein scaffolds through metal-dependent polymorphism. Authors: Liutkus, M. / Sasselli, I.R. / Rojas, A.L. / Cortajarena, A.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 45.3 KB | Display | ![]() |
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PDB format | ![]() | 30.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.2 MB | Display | ![]() |
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Full document | ![]() | 2.2 MB | Display | |
Data in XML | ![]() | 4.6 KB | Display | |
Data in CIF | ![]() | 6.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8bu0C ![]() 8chyC ![]() 8cigC ![]() 8ckrC ![]() 8cmqC ![]() 8cp8C ![]() 8cqpC ![]() 8cqqC ![]() 8ot7C C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16554.691 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() | ||||||||
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#2: Chemical | #3: Chemical | ChemComp-TRS / | #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 52.31 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion / pH: 8 / Details: 5mM GdCl3 100 mM Tris HCl 30% MPD |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 16, 2020 / Details: Elliptically bent mirror |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.711822 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→57.73 Å / Num. obs: 20384 / % possible obs: 100 % / Redundancy: 11.5 % / CC1/2: 0.997 / Rpim(I) all: 0.031 / Rrim(I) all: 0.106 / Net I/σ(I): 13.2 / Num. measured all: 234292 |
Reflection shell | Resolution: 1.75→1.84 Å / % possible obs: 100 % / Redundancy: 11 % / Num. measured all: 32240 / Num. unique obs: 2930 / CC1/2: 0.958 / Rpim(I) all: 0.207 / Rrim(I) all: 0.687 / Net I/σ(I) obs: 3.1 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 38.778 Å2
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Refinement step | Cycle: 1 / Resolution: 1.75→46.03 Å
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Refine LS restraints |
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