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- PDB-8ckr: Crystal structure of an 8-repeat consensus TPR superhelix with in... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8ckr | ||||||
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Title | Crystal structure of an 8-repeat consensus TPR superhelix with in Hepes with Ca | ||||||
![]() | Consensus tetratricopeptide repeat protein | ||||||
![]() | DE NOVO PROTEIN / CTPR / polymorphism / metal coordination. | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liutkus, M. / Rojas, A.L. / Cortajarena, A.L. | ||||||
Funding support | European Union, 1items
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![]() | ![]() Title: Diverse crystalline protein scaffolds through metal-dependent polymorphism. Authors: Liutkus, M. / Sasselli, I.R. / Rojas, A.L. / Cortajarena, A.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 49.7 KB | Display | ![]() |
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PDB format | ![]() | 32.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3.8 MB | Display | ![]() |
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Full document | ![]() | 3.8 MB | Display | |
Data in XML | ![]() | 8.7 KB | Display | |
Data in CIF | ![]() | 11.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 34186.594 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() | ||||||
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#2: Chemical | ChemComp-CA / #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 292 K / Method: vapor diffusion / pH: 7.5 / Details: 20mM CaCl2 100 mM Hepes 30% MPD |
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-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 25, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97926 Å / Relative weight: 1 |
Reflection | Resolution: 1.3→59.17 Å / Num. obs: 48472 / % possible obs: 100 % / Redundancy: 15 % / CC1/2: 0.999 / Rpim(I) all: 0.022 / Rrim(I) all: 0.087 / Net I/σ(I): 11 / Num. measured all: 729150 |
Reflection shell | Resolution: 1.3→1.37 Å / % possible obs: 100 % / Redundancy: 14.5 % / Num. measured all: 101260 / Num. unique obs: 6993 / CC1/2: 0.438 / Rpim(I) all: 0.605 / Rrim(I) all: 2.322 / Net I/σ(I) obs: 1.3 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.941 Å2
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Refinement step | Cycle: 1 / Resolution: 1.3→59.16 Å
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Refine LS restraints |
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