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- PDB-7yxi: Drosophila melanogaster JMJD7 (dmJMJD7) in complex with Mn and N-... -

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Basic information

Entry
Database: PDB / ID: 7yxi
TitleDrosophila melanogaster JMJD7 (dmJMJD7) in complex with Mn and N-oxalylglycine (NOG)
ComponentsGH14974p
KeywordsOXIDOREDUCTASE / NON-HEME / IRON / 2-OXOGLUTARATE / DIOXYGENASE / OXYGENASE / JMJC / JMJC DOMAIN / JMJC DOMAIN-CONTAINING PROTEIN 7 / JMJD7 / JMJC HYDROXYLASE / JMJC DEMETHYLASE / KDMS / POST-TRANSLATIONAL MODIFICATIONS / PTM / HYDROXYLATION / LYSYL HYDROXYLATION / DIMERISATION / TRANSLATION FACTOR / DEVELOPMENTALLY REGULATED GTP BINDING PROTEINS / DRG1 / DRG2 / TRAFAC GTPASE / HYPOXIA / NUCLEIC ACID- BINDING / METAL-BINDING / TRANSLATION / HELIX-LOOP-HELIX-BETA / DSBH / FACIAL TRIAD / DEVELOPMENT / CANCER / RIBOSOME BIOGENESIS
Function / homology
Function and homology information


peptidyl-lysine 3-dioxygenase activity / Protein hydroxylation / wybutosine biosynthetic process / Oxidoreductases; Acting on paired donors, with incorporation or reduction of molecular oxygen; With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor / histone H3K36 demethylase activity / 2-oxoglutarate-dependent dioxygenase activity / phospholipase A2 / tRNA binding / hydrolase activity / nucleus ...peptidyl-lysine 3-dioxygenase activity / Protein hydroxylation / wybutosine biosynthetic process / Oxidoreductases; Acting on paired donors, with incorporation or reduction of molecular oxygen; With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor / histone H3K36 demethylase activity / 2-oxoglutarate-dependent dioxygenase activity / phospholipase A2 / tRNA binding / hydrolase activity / nucleus / metal ion binding / cytoplasm
Similarity search - Function
Cupin-like domain 8 / Cupin-like domain / A domain family that is part of the cupin metalloenzyme superfamily. / JmjC domain / JmjC domain profile. / RmlC-like jelly roll fold
Similarity search - Domain/homology
: / N-OXALYLGLYCINE / GH14974p
Similarity search - Component
Biological speciesDrosophila melanogaster (fruit fly)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å
AuthorsChowdhury, R. / Schofield, C.J.
Funding support United Kingdom, 1items
OrganizationGrant numberCountry
Wellcome Trust106244/Z/14/Z United Kingdom
Citation
Journal: Sci Rep / Year: 2022
Title: Conservation of the unusual dimeric JmjC fold of JMJD7 from Drosophila melanogaster to humans.
Authors: Chowdhury, R. / Abboud, M.I. / Wiley, J. / Tumber, A. / Markolovic, S. / Schofield, C.J.
#1: Journal: Nat Chem Biol / Year: 2018
Title: The Jumonji-C oxygenase JMJD7 catalyzes (3S)-lysyl hydroxylation of TRAFAC GTPases.
Authors: Markolovic, S. / Zhuang, Q. / Wilkins, S.E. / Eaton, C.D. / Abboud, M.I. / Katz, M.J. / McNeil, H.E. / Lesniak, R.K. / Hall, C. / Struwe, W.B. / Konietzny, R. / Davis, S. / Yang, M. / Ge, W. ...Authors: Markolovic, S. / Zhuang, Q. / Wilkins, S.E. / Eaton, C.D. / Abboud, M.I. / Katz, M.J. / McNeil, H.E. / Lesniak, R.K. / Hall, C. / Struwe, W.B. / Konietzny, R. / Davis, S. / Yang, M. / Ge, W. / Benesch, J.L.P. / Kessler, B.M. / Ratcliffe, P.J. / Cockman, M.E. / Fischer, R. / Wappner, P. / Chowdhury, R. / Coleman, M.L. / Schofield, C.J.
History
DepositionFeb 16, 2022Deposition site: PDBE / Processing site: PDBE
Revision 1.0Apr 27, 2022Provider: repository / Type: Initial release
Revision 1.1May 4, 2022Group: Refinement description / Category: refine_ls_restr
Revision 1.2Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: GH14974p
B: GH14974p
hetero molecules


Theoretical massNumber of molelcules
Total (without water)74,0219
Polymers73,3972
Non-polymers6247
Water8,431468
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5240 Å2
ΔGint-55 kcal/mol
Surface area25570 Å2
MethodPISA
Unit cell
Length a, b, c (Å)55.688, 64.930, 103.150
Angle α, β, γ (deg.)90.000, 102.630, 90.000
Int Tables number4
Space group name H-MP1211

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein GH14974p / Jumonji domain containing 7


Mass: 36698.309 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: JMJD7, Dmel\CG10133, CG10133, Dmel_CG10133 / Plasmid: PET28A(+) / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: Q9VU77, Oxidoreductases; Acting on paired donors, with incorporation or reduction of molecular oxygen; With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

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Non-polymers , 5 types, 475 molecules

#2: Chemical ChemComp-MN / MANGANESE (II) ION


Mass: 54.938 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mn
#3: Chemical ChemComp-OGA / N-OXALYLGLYCINE


Mass: 147.086 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C4H5NO5 / Feature type: SUBJECT OF INVESTIGATION / Comment: inhibitor*YM
#4: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C2H6O2
#5: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: SO4
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 468 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.55 Å3/Da / Density % sol: 51.87 %
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop / pH: 5.4
Details: Sample: 16.0 mg/ml dmJMJD7 (in 50 mM Hepes-Na pH 7.5, 200 mM NaCl, 5% glycerol), 2.0 mM MnCl2 and 4.0 mM compound. Reservoir: 0.1 M Bis-Tris pH 5.4, 0.3 M magnesium chloride, 0.1 M ammonium ...Details: Sample: 16.0 mg/ml dmJMJD7 (in 50 mM Hepes-Na pH 7.5, 200 mM NaCl, 5% glycerol), 2.0 mM MnCl2 and 4.0 mM compound. Reservoir: 0.1 M Bis-Tris pH 5.4, 0.3 M magnesium chloride, 0.1 M ammonium acetate (or alternatively, 0.1 M barium chloride), and 0.002 M manganese chloride. Cryo-protection: 20% v/v ethylene glycol.

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.9795 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jul 23, 2017 / Details: MIRRORS
RadiationMonochromator: SI 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9795 Å / Relative weight: 1
ReflectionResolution: 2.1→50 Å / Num. obs: 42025 / % possible obs: 99.9 % / Redundancy: 5 % / Biso Wilson estimate: 28.1 Å2 / CC1/2: 0.996 / Rpim(I) all: 0.063 / Rrim(I) all: 0.142 / Rsym value: 0.126 / Χ2: 1.042 / Net I/σ(I): 4.9
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Num. unique obsCC1/2Rpim(I) allRrim(I) allRsym valueΧ2% possible all
2.1-2.184.941630.5410.420.940.8371.04299.5
2.18-2.26541650.70.3270.7330.6531.0399.7
2.26-2.374.941510.7930.2450.5380.4761.07199.8
2.37-2.495.142140.870.1880.4230.3771.06499.9
2.49-2.65541870.9060.1490.3320.2941.023100
2.65-2.855.241860.9490.1020.2310.2071.027100
2.85-3.145.141850.9770.0720.1620.1441.052100
3.14-3.595.142180.9880.0470.1070.0961.038100
3.59-4.525.142310.9950.0310.070.0631.037100
4.52-50543250.9960.0250.0560.051.03299.8

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Processing

Software
NameVersionClassification
SCALEPACKdata scaling
PHENIX1.14_3260refinement
PDB_EXTRACT3.27data extraction
HKL-2000data reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7YXG

7yxg


Resolution: 2.1→43.969 Å / SU ML: 0.23 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 18.96 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2034 2057 4.91 %
Rwork0.1717 39866 -
obs0.1732 41923 99.49 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 132.46 Å2 / Biso mean: 38.3507 Å2 / Biso min: 9.14 Å2
Refinement stepCycle: final / Resolution: 2.1→43.969 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4929 0 53 468 5450
Biso mean--28.07 37.81 -
Num. residues----624
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0125194
X-RAY DIFFRACTIONf_angle_d1.1367115
X-RAY DIFFRACTIONf_dihedral_angle_d4.4984200
X-RAY DIFFRACTIONf_chiral_restr0.061772
X-RAY DIFFRACTIONf_plane_restr0.009938
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.1-2.14890.29161480.2442248894
2.1489-2.20260.24781470.2271259999
2.2026-2.26220.24991380.20872660100
2.2622-2.32870.22821620.1972627100
2.3287-2.40390.24921520.19482652100
2.4039-2.48980.2521240.18782661100
2.4898-2.58950.25221450.19082652100
2.5895-2.70730.22491360.18542669100
2.7073-2.850.2061070.1662676100
2.85-3.02860.20741330.172678100
3.0286-3.26230.1851600.16212651100
3.2623-3.59050.17341330.152681100
3.5905-4.10970.16161290.14072686100
4.1097-5.17650.16631130.13412729100
5.1765-43.9690.20811300.2049275799
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.19761.63030.36657.0146-0.59322.81960.05630.0434-0.12750.1601-0.03940.89260.4767-0.4520.04870.24-0.06930.01240.217-0.03150.2873-29.1693-3.9844-18.2438
22.46051.5530.54641.33530.89351.3287-0.09670.39620.2277-0.23120.09870.2693-0.052-0.0220.0760.21110.0148-0.01110.20240.00220.2595-17.830614.4031-20.9359
31.93640.95291.01541.69030.08312.8661-0.0083-0.04250.21190.2243-0.04850.1225-0.3316-0.0326-0.01350.21460.0016-0.00050.1831-0.00220.239-7.587814.4209-16.8387
41.5843-0.5106-1.00740.74850.5541.167-0.16860.24450.8895-0.55280.21960.1517-0.25740.3737-0.12710.4648-0.1103-0.03660.41040.05950.26391.069423.4732-33.6929
56.55663.73413.44144.4142-1.3197.7235-0.320.2791-0.7123-1.24450.3725-0.8137-0.47560.96170.20360.5643-0.21440.13260.6859-0.05030.52828.149718.8164-47.7687
62.45210.589-2.64620.8272-0.64392.9853-0.19580.5131-0.3867-0.44520.2634-0.26820.26870.314-0.16060.4445-0.07240.03670.5615-0.07150.3112-5.4835.2847-49.0622
71.770.90380.10812.00740.8992.2387-0.22170.7955-0.3626-0.59650.24240.0947-0.05420.0728-0.03250.5513-0.0514-0.04730.5421-0.09780.3513-16.9628-5.5373-49.4221
81.18650.9075-0.27440.4811-0.02590.1198-0.07660.59970.0799-0.63180.2858-0.371-0.03320.4386-0.17180.6756-0.17310.09410.7018-0.07370.4044-0.07889.7293-53.1497
93.97491.42470.30942.42681.31733.4913-0.13910.5930.04-0.3010.2456-0.17990.52170.4672-0.05170.4808-0.08250.04770.6057-0.06510.3206-1.28739.3062-45.8445
102.94482.1922-1.83362.7521-2.02814.8006-0.0979-0.0861-0.3115-0.0441-0.1227-0.319-0.09830.8940.08780.2618-0.054-0.00580.3798-0.02490.2945.623114.8206-26.5433
110.97650.6055-0.28363.0207-1.72033.5212-0.0305-0.1501-0.4828-0.32190.0434-0.09450.37750.67470.09880.28820.1160.07860.4160.08260.3492.34667.6094-31.546
122.16150.4374-0.41371.04251.3682.5996-0.25640.46510.0212-0.27250.19290.1977-0.1475-0.1859-0.00020.3465-0.0587-0.07950.36920.00040.2423-11.6236.6693-39.9427
132.05721.6931-0.28132.0749-0.86442.9687-0.15820.230.0935-0.35480.03040.00250.01310.1046-0.00060.26720.0015-0.03040.23130.02630.2134-8.463312.4681-32.5436
140.3834-0.08830.10460.53730.5492.439-0.2270.305-0.3327-0.33280.1544-0.00910.26440.25490.0790.3647-0.09450.00070.3319-0.08690.2185-17.153-3.77-37.7744
153.79670.9494-0.27741.60740.81394.379-0.01721.0335-1.0514-0.8163-0.2488-0.30120.93631.21890.1730.8185-0.06410.11850.6305-0.22630.4967-8.6613-9.0419-54.2886
163.17780.7052-0.61534.15180.48963.8304-0.12360.3349-0.0622-0.07580.09680.12360.3426-0.23690.00940.3898-0.0822-0.0460.3743-0.06890.2205-18.8376-1.739-39.2711
172.60641.3060.03031.5791-0.19862.2343-0.21060.51240.5201-0.25150.27710.77860.1368-0.47960.02050.2774-0.0162-0.06530.3690.04570.3798-24.69016.4615-33.1994
181.76521.32550.1072.2443-0.83112.52320.0470.2014-0.0706-0.23470.0037-0.37880.15870.0333-0.03040.2180.015-0.00620.2323-0.02390.2332-8.956710.0508-26.5053
190.6059-0.06941.08352.4904-0.19052.6712-0.42840.53850.0915-0.66640.2576-0.09580.1364-0.1564-0.03840.3401-0.0366-0.04920.34040.03950.2304-11.391112.1607-39.7022
201.85150.0692.22093.0153-2.65894.7826-0.03050.0229-0.2177-0.12250.1314-0.1350.09830.22930.04260.2380.01850.01650.4145-0.01270.2489-0.31939.194-31.3929
214.54161.55780.02283.8264-1.2252.86970.51490.47440.4275-0.0164-0.3234-0.180.40170.1499-0.08450.39280.08880.09180.26480.03850.382-12.7517-6.7841-20.75
228.46221.22191.58392.0536-0.3933.1560.24520.8005-1.3974-0.47640.0268-0.20680.61330.1022-0.27090.5036-0.03190.02290.2213-0.08670.2836-14.2098-16.0735-17.1877
232.87091.66191.05242.18340.85261.8254-0.1440.5801-0.14930.1920.25890.06370.3750.29440.02290.2913-0.00130.06660.2295-0.01750.3596-1.16-6.6962-4.1174
241.82460.3199-0.40592.4168-0.56932.4479-0.16370.329-0.0632-0.23180.0341-0.3401-0.04840.2930.07910.1918-0.01210.03930.20170.00950.2837-0.7193.5157-4.3371
252.2392-0.7662-1.11161.95721.19183.1124-0.1928-0.74850.18160.25940.215-0.2473-0.25730.9103-0.02570.2447-0.0899-0.05290.3418-0.05110.32330.30486.841715.995
263.4959-0.0879-2.88231.0944-0.25172.85030.3019-1.07890.77940.0199-0.3110.1952-0.4035-0.0781-0.1120.5171-0.15350.05310.5902-0.2090.4639-11.23579.417228.4816
272.12270.24690.15841.74630.17342.0887-0.1097-0.33610.28250.1223-0.00140.0819-0.037-0.0621-0.02740.2301-0.00460.00480.2913-0.02850.299-22.139-3.929620.011
282.63652.4621-0.86863.1574-1.14511.2568-0.17570.2698-0.1649-0.20590.2346-0.07580.2392-0.1518-0.00730.1935-0.0106-0.00940.25270.03680.3279-30.8362-14.669512.5815
293.47780.17490.03912.23321.49651.0832-0.1715-0.99740.61630.27250.11450.286-0.29790.08980.02690.3246-0.00530.0070.5383-0.10060.3565-20.3150.362127.7082
303.12140.4939-1.01115.2447-1.63114.22190.0728-0.09720.32270.06560.46240.4037-0.479-0.565-0.15870.25480.01510.06450.3363-0.01830.3174-17.96551.102320.1962
310.37970.7969-0.29842.11750.1814.85240.0743-0.10570.28220.24340.0050.0244-0.6895-0.0248-0.09750.3446-0.03150.00820.2675-0.03250.3094-5.507613.277.9409
320.51320.9071-1.56641.6815-2.73964.94080.0909-0.08740.25870.16330.17720.1963-0.9093-0.4904-0.21860.38520.08150.04010.2968-0.00370.2761-14.2229.15158.2459
331.84540.7649-0.75822.6813-0.21810.9231-0.0818-0.1387-0.0935-0.0322-0.05720.06570.2222-0.2281-0.10310.170.0031-0.00820.220.02560.2532-16.6741-6.990611.1618
340.87060.4965-0.08341.7664-0.11822.7909-0.0648-0.0187-0.08170.04020.0442-0.0467-0.07140.1068-0.01010.1486-0.01230.02350.1778-0.00330.2159-8.5884-1.77038.2103
351.90391.0924-0.32530.9491-0.74190.9077-0.11120.34710.0903-0.20820.10040.09590.0931-0.3938-0.0130.2161-0.0284-0.0120.18650.03350.2514-24.9574-11.07684.4683
365.1930.2354-0.41954.20961.81851.181-0.24560.02440.35330.38370.05660.9857-0.3329-0.8271-0.18020.29510.00440.08570.40720.06620.4958-37.3687-9.04217.5519
372.45190.03110.68482.9151-0.39892.695-0.1119-0.38510.0339-0.48040.44690.13540.3707-0.2709-0.11690.2665-0.0445-0.00960.26970.06390.2315-22.8904-13.48944.955
382.07110.63710.45693.25070.1452.0683-0.04780.1472-0.3614-0.28590.098-0.07770.43930.0669-0.04410.29090.00660.04940.1678-0.01890.2997-12.2749-17.21491.5689
391.45310.92880.74691.7906-0.54981.8383-0.1027-0.07920.3288-0.1845-0.0721-0.04420.0474-0.0224-0.00560.2192-0.01130.02890.182-0.03890.2411-8.3295-0.26661.7891
401.0963-0.1376-0.39630.9521-0.87291.9972-0.0172-0.0663-0.1208-0.06120.0230.01090.06460.3365-0.06660.1702-0.02010.00110.1859-0.00710.2367-11.4691-6.796813.4086
410.7536-0.17850.94932.1118-2.38034.83430.0402-0.00420.05240.0487-0.0751-0.0784-0.2173-0.2352-0.00640.27030.01070.02930.2232-0.03210.2719-12.01776.37348.2317
421.33070.95210.98824.46890.47763.1529-0.0218-0.1284-0.01050.24330.2980.20140.33350.0247-0.18120.2918-0.0025-0.02250.2964-0.00860.2829-21.6745-2.9068-11.1372
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1(chain A and resid 1:18)A1 - 18
2X-RAY DIFFRACTION2(chain A and resid 19:27)A19 - 27
3X-RAY DIFFRACTION3(chain A and resid 28:49)A28 - 49
4X-RAY DIFFRACTION4(chain A and resid 50:62)A50 - 62
5X-RAY DIFFRACTION5(chain A and resid 63:73)A63 - 73
6X-RAY DIFFRACTION6(chain A and resid 74:86)A74 - 86
7X-RAY DIFFRACTION7(chain A and resid 87:99)A87 - 99
8X-RAY DIFFRACTION8(chain A and resid 100:114)A100 - 114
9X-RAY DIFFRACTION9(chain A and resid 115:135)A115 - 135
10X-RAY DIFFRACTION10(chain A and resid 136:154)A136 - 154
11X-RAY DIFFRACTION11(chain A and resid 155:165)A155 - 165
12X-RAY DIFFRACTION12(chain A and resid 166:178)A166 - 178
13X-RAY DIFFRACTION13(chain A and resid 179:195)A179 - 195
14X-RAY DIFFRACTION14(chain A and resid 196:215)A196 - 215
15X-RAY DIFFRACTION15(chain A and resid 216:234)A216 - 234
16X-RAY DIFFRACTION16(chain A and resid 235:245)A235 - 245
17X-RAY DIFFRACTION17(chain A and resid 246:265)A246 - 265
18X-RAY DIFFRACTION18(chain A and resid 266:276)A266 - 276
19X-RAY DIFFRACTION19(chain A and resid 277:286)A277 - 286
20X-RAY DIFFRACTION20(chain A and resid 287:294)A287 - 294
21X-RAY DIFFRACTION21(chain A and resid 295:312)A295 - 312
22X-RAY DIFFRACTION22(chain B and resid 1:18)B1 - 18
23X-RAY DIFFRACTION23(chain B and resid 19:27)B19 - 27
24X-RAY DIFFRACTION24(chain B and resid 28:49)B28 - 49
25X-RAY DIFFRACTION25(chain B and resid 50:62)B50 - 62
26X-RAY DIFFRACTION26(chain B and resid 63:73)B63 - 73
27X-RAY DIFFRACTION27(chain B and resid 74:86)B74 - 86
28X-RAY DIFFRACTION28(chain B and resid 87:99)B87 - 99
29X-RAY DIFFRACTION29(chain B and resid 100:114)B100 - 114
30X-RAY DIFFRACTION30(chain B and resid 115:135)B115 - 135
31X-RAY DIFFRACTION31(chain B and resid 136:154)B136 - 154
32X-RAY DIFFRACTION32(chain B and resid 155:165)B155 - 165
33X-RAY DIFFRACTION33(chain B and resid 166:178)B166 - 178
34X-RAY DIFFRACTION34(chain B and resid 179:195)B179 - 195
35X-RAY DIFFRACTION35(chain B and resid 196:215)B196 - 215
36X-RAY DIFFRACTION36(chain B and resid 216:234)B216 - 234
37X-RAY DIFFRACTION37(chain B and resid 235:245)B235 - 245
38X-RAY DIFFRACTION38(chain B and resid 246:265)B246 - 265
39X-RAY DIFFRACTION39(chain B and resid 266:276)B266 - 276
40X-RAY DIFFRACTION40(chain B and resid 277:286)B277 - 286
41X-RAY DIFFRACTION41(chain B and resid 287:294)B287 - 294
42X-RAY DIFFRACTION42(chain B and resid 295:312)B295 - 312

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