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- PDB-7vph: Crystal structure of the C-terminal tail of SARS-CoV-2 Orf6 compl... -

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Basic information

Entry
Database: PDB / ID: 7vph
TitleCrystal structure of the C-terminal tail of SARS-CoV-2 Orf6 complex with human nucleoporin pair Rae1-Nup98
Components
  • Isoform 3 of Nuclear pore complex protein Nup98-Nup96
  • ORF6 protein
  • mRNA export factor
KeywordsVIRAL PROTEIN / Complex / Coronavirus / RNA nuclear export / Protein binding
Function / homology
Function and homology information


Translation of Accessory Proteins / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore complex assembly / telomere tethering at nuclear periphery / nuclear pore outer ring / nuclear pore organization / nuclear pore cytoplasmic filaments / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / Nuclear Pore Complex (NPC) Disassembly / nuclear inclusion body ...Translation of Accessory Proteins / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore complex assembly / telomere tethering at nuclear periphery / nuclear pore outer ring / nuclear pore organization / nuclear pore cytoplasmic filaments / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / Nuclear Pore Complex (NPC) Disassembly / nuclear inclusion body / nuclear pore nuclear basket / Regulation of Glucokinase by Glucokinase Regulatory Protein / Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) / Transport of Ribonucleoproteins into the Host Nucleus / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / NS1 Mediated Effects on Host Pathways / SUMOylation of SUMOylation proteins / nuclear localization sequence binding / structural constituent of nuclear pore / Transport of Mature mRNA Derived from an Intronless Transcript / positive regulation of mRNA splicing, via spliceosome / Rev-mediated nuclear export of HIV RNA / Nuclear import of Rev protein / SUMOylation of RNA binding proteins / NEP/NS2 Interacts with the Cellular Export Machinery / Transport of Mature mRNA derived from an Intron-Containing Transcript / RNA export from nucleus / tRNA processing in the nucleus / Postmitotic nuclear pore complex (NPC) reformation / nucleocytoplasmic transport / Viral Messenger RNA Synthesis / mitotic spindle pole / SUMOylation of ubiquitinylation proteins / Vpr-mediated nuclear import of PICs / host cell Golgi membrane / SUMOylation of DNA replication proteins / Hydrolases; Acting on peptide bonds (peptidases); Serine endopeptidases / Regulation of HSF1-mediated heat shock response / mRNA transport / nuclear pore / mRNA export from nucleus / SUMOylation of DNA damage response and repair proteins / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / serine-type peptidase activity / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / nuclear periphery / protein sequestering activity / regulation of mitotic spindle organization / Resolution of Sister Chromatid Cohesion / SUMOylation of chromatin organization proteins / ubiquitin binding / HCMV Late Events / promoter-specific chromatin binding / molecular condensate scaffold activity / Transcriptional regulation by small RNAs / RHO GTPases Activate Formins / ISG15 antiviral mechanism / fibrillar center / protein import into nucleus / HCMV Early Events / Separation of Sister Chromatids / nuclear envelope / snRNP Assembly / nuclear membrane / symbiont-mediated suppression of host ISG15-protein conjugation / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity / microtubule binding / transcription coactivator activity / nuclear body / host cell endoplasmic reticulum membrane / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / ribonucleoprotein complex / cell division / mRNA binding / SARS-CoV-2 activates/modulates innate and adaptive immune responses / proteolysis / RNA binding / nucleoplasm / nucleus / membrane / cytosol / cytoplasm
Similarity search - Function
Non-structural protein NS6, betacoronavirus / Betacoronavirus NS6 protein / Nup98, Gle2-binding sequence / Nuclear pore complex protein NUP96, C-terminal domain / Nuclear protein 96 / Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain / Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain superfamily / Nucleoporin peptidase S59-like / Nup98-96 autopeptidase S59 / NUP C-terminal domain profile. ...Non-structural protein NS6, betacoronavirus / Betacoronavirus NS6 protein / Nup98, Gle2-binding sequence / Nuclear pore complex protein NUP96, C-terminal domain / Nuclear protein 96 / Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain / Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain superfamily / Nucleoporin peptidase S59-like / Nup98-96 autopeptidase S59 / NUP C-terminal domain profile. / YVTN repeat-like/Quinoprotein amine dehydrogenase / 7 Propeller / Methylamine Dehydrogenase; Chain H / WD domain, G-beta repeat / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Mainly Beta
Similarity search - Domain/homology
ORF6 protein / Nuclear pore complex protein Nup98-Nup96 / mRNA export factor RAE1
Similarity search - Component
Biological speciesHomo sapiens (human)
Severe acute respiratory syndrome coronavirus 2
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å
AuthorsLi, T. / Guo, H. / Yang, T. / Wen, Y. / Ji, X.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)81871639 China
CitationJournal: Front Mol Biosci / Year: 2021
Title: Molecular Mechanism of SARS-CoVs Orf6 Targeting the Rae1-Nup98 Complex to Compete With mRNA Nuclear Export.
Authors: Li, T. / Wen, Y. / Guo, H. / Yang, T. / Yang, H. / Ji, X.
History
DepositionOct 17, 2021Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jan 19, 2022Provider: repository / Type: Initial release
Revision 1.1Feb 16, 2022Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID
Revision 1.2Nov 29, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: mRNA export factor
B: Isoform 3 of Nuclear pore complex protein Nup98-Nup96
C: mRNA export factor
D: Isoform 3 of Nuclear pore complex protein Nup98-Nup96
E: mRNA export factor
F: Isoform 3 of Nuclear pore complex protein Nup98-Nup96
G: mRNA export factor
H: Isoform 3 of Nuclear pore complex protein Nup98-Nup96
I: ORF6 protein
J: ORF6 protein
K: ORF6 protein
X: ORF6 protein


Theoretical massNumber of molelcules
Total (without water)210,68412
Polymers210,68412
Non-polymers00
Water1,00956
1
A: mRNA export factor
B: Isoform 3 of Nuclear pore complex protein Nup98-Nup96
X: ORF6 protein


Theoretical massNumber of molelcules
Total (without water)52,6713
Polymers52,6713
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4080 Å2
ΔGint-15 kcal/mol
Surface area17070 Å2
MethodPISA
2
C: mRNA export factor
D: Isoform 3 of Nuclear pore complex protein Nup98-Nup96
I: ORF6 protein


Theoretical massNumber of molelcules
Total (without water)52,6713
Polymers52,6713
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3820 Å2
ΔGint-17 kcal/mol
Surface area16430 Å2
MethodPISA
3
E: mRNA export factor
F: Isoform 3 of Nuclear pore complex protein Nup98-Nup96
J: ORF6 protein


Theoretical massNumber of molelcules
Total (without water)52,6713
Polymers52,6713
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3970 Å2
ΔGint-15 kcal/mol
Surface area17140 Å2
MethodPISA
4
G: mRNA export factor
H: Isoform 3 of Nuclear pore complex protein Nup98-Nup96
K: ORF6 protein


Theoretical massNumber of molelcules
Total (without water)52,6713
Polymers52,6713
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3820 Å2
ΔGint-18 kcal/mol
Surface area16760 Å2
MethodPISA
Unit cell
Length a, b, c (Å)150.657, 103.319, 136.588
Angle α, β, γ (deg.)90.00, 97.45, 90.00
Int Tables number5
Space group name H-MC121

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Components

#1: Protein
mRNA export factor / Rae1 protein homolog / mRNA-associated protein mrnp 41


Mass: 42398.527 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: RAE1, MRNP41 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P78406
#2: Protein
Isoform 3 of Nuclear pore complex protein Nup98-Nup96


Mass: 7785.728 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: NUP98, ADAR2 / Production host: Spodoptera frugiperda (fall armyworm)
References: UniProt: P52948, Hydrolases; Acting on peptide bonds (peptidases); Serine endopeptidases
#3: Protein/peptide
ORF6 protein / ORF6 / Accessory protein 6 / Non-structural protein 6 / ns6 / Protein X3


Mass: 2486.661 Da / Num. of mol.: 4 / Source method: obtained synthetically
Source: (synth.) Severe acute respiratory syndrome coronavirus 2
References: UniProt: P0DTC6
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 56 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.5 Å3/Da / Density % sol: 50.83 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: 100 mM Sodium citrate pH 5.5, 20% PEG 3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.979 Å
DetectorType: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jan 23, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 2.796→41.065 Å / Num. obs: 51228 / % possible obs: 99.32 % / Redundancy: 3.3 % / CC1/2: 0.99 / Net I/σ(I): 1
Reflection shellResolution: 2.8→2.85 Å / Num. unique obs: 5087 / CC1/2: 0.58

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Processing

Software
NameVersionClassification
PHENIX(1.19.2_4158: ???)refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3MMY

3mmy


Resolution: 2.8→41.06 Å / SU ML: 0.43 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 28.88 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2442 1992 3.89 %
Rwork0.1924 --
obs0.1945 51064 99.36 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.8→41.06 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12503 0 0 56 12559
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0112839
X-RAY DIFFRACTIONf_angle_d1.3817416
X-RAY DIFFRACTIONf_dihedral_angle_d7.7661672
X-RAY DIFFRACTIONf_chiral_restr0.0711855
X-RAY DIFFRACTIONf_plane_restr0.0112278
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.8-2.870.41351390.34553441X-RAY DIFFRACTION97
2.87-2.940.37831420.32113505X-RAY DIFFRACTION100
2.94-3.030.32681410.28963490X-RAY DIFFRACTION100
3.03-3.130.32921440.26913543X-RAY DIFFRACTION100
3.13-3.240.31921390.26063462X-RAY DIFFRACTION99
3.24-3.370.29611440.2453528X-RAY DIFFRACTION100
3.37-3.520.29471430.22783527X-RAY DIFFRACTION100
3.52-3.710.25021410.20093474X-RAY DIFFRACTION100
3.71-3.940.26181420.18173527X-RAY DIFFRACTION100
3.94-4.240.23211440.16293528X-RAY DIFFRACTION100
4.24-4.670.18211430.13163535X-RAY DIFFRACTION100
4.67-5.350.15941450.13223557X-RAY DIFFRACTION100
5.35-6.730.28071430.16713547X-RAY DIFFRACTION100
6.73-41.060.18521420.17563520X-RAY DIFFRACTION97
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.93151.831.8852.38381.34881.3058-0.38230.4791-0.0174-0.48740.2797-0.1191-0.25240.92130.03560.4542-0.05990.15530.7646-0.02190.7499-15.939626.3828-1.2791
22.88820.18150.13923.6622-1.20112.87350.02320.0134-0.1052-0.0983-0.017-0.5559-0.19960.4026-0.02310.27730.04730.06770.4675-0.04660.4262-22.239813.33346.5938
32.0341-1.264-1.11194.90691.34392.8492-0.07460.0651-0.24870.08540.02470.39920.3822-0.06430.10210.3421-0.0338-0.02210.3988-0.01760.3033-38.771912.33712.2218
41.8882-0.0613-0.73155.1342-1.30182.92620.1045-0.28190.03530.35270.05620.2151-0.1175-0.1429-0.14910.3056-0.0196-0.03310.3954-0.01320.2966-38.536930.014420.6294
53.14391.28820.16993.55042.14961.58430.09650.32270.3755-0.6301-0.1888-0.1554-0.3989-0.02660.01840.4471-0.03160.09910.34420.00340.4333-28.557937.94999.1268
63.1610.45080.51274.0344-0.31093.92110.0572-0.16480.45760.3554-0.1822-0.4549-0.52960.196-0.05310.4929-0.10680.0460.4019-0.03160.5256-24.830738.256113.9097
73.24110.8280.83963.38090.85862.76730.20320.3118-0.0469-0.65620.0618-0.8277-0.06390.5329-0.25590.48670.00480.15360.4878-0.02790.5528-22.281629.22543.3081
83.09210.4572-1.5885.51793.75883.75030.2919-0.3183-0.17270.22940.30690.5371-0.3372-0.6216-0.56150.78640.0708-0.0510.64850.17120.4956-41.781338.7644-5.5791
97.94993.02023.50897.37422.0112.26720.2348-0.57840.16920.23390.6872-2.0329-2.3681-1.2966-0.68381.35610.18840.0520.5728-0.03110.8737-38.409350.33554.5794
105.32961.74710.63018.4014-1.70621.65470.04450.27530.1317-0.25590.1920.11770.2472-0.1788-0.16030.57220.0691-0.10230.50460.01830.2314-41.0427.1914-6.1004
112.4664-0.82830.96721.59730.46561.8408-0.20110.2935-0.46830.2492-0.10290.78290.3982-0.79080.26060.5505-0.2920.04840.86420.04111.2859-2.625844.681925.1695
125.17290.6425-0.42940.13150.16290.9491-0.01120.4903-0.312-0.53850.06411.04310.2637-0.66240.11270.5435-0.1931-0.16860.67530.03481.12423.262349.173215.6113
133.86170.76871.31382.8980.89224.8938-0.08880.1219-0.1488-0.09240.13010.4511-0.06440.1029-0.00660.363-0.09130.00840.3675-0.0060.440419.340957.596923.6392
142.8798-1.8286-1.43122.13981.64991.24770.30260.43780.0890.7651-0.5786-0.14810.0217-0.36580.29880.7425-0.10350.29140.47440.03590.743113.373957.661937.9935
152.43051.7046-0.12912.4633-0.21973.08040.1529-0.4558-0.1430.9257-0.12250.68940.4682-0.2021-0.07760.8959-0.14520.37560.55080.0151.00578.193252.045344.9032
161.3176-0.6899-0.99270.62060.72332.9781-0.00070.2342-0.19510.4762-0.20650.84380.9832-0.91030.1690.7317-0.30650.23170.8093-0.03091.413-0.036142.524234.7729
170.08610.0789-0.01750.2535-0.15030.10020.1753-0.35620.65971.0479-0.44061.70470.68630.2317-0.21591.1052-0.03140.08920.8460.03360.939421.964928.086331.2805
181.93521.3736-0.07021.6744-0.70560.49390.1597-0.4319-0.23670.71790.1508-0.05810.96910.2248-0.20321.38010.06930.22160.57580.08721.10819.834834.28942.2349
197.1389-0.5921-1.47754.4099-4.70175.6837-0.29790.936-1.04730.08490.12220.80961.8933-0.44730.13191.142-0.14320.10870.4708-0.10040.957216.122932.481125.4768
205.8036-0.23530.12640.97840.10412.68220.24650.47481.2756-0.02610.1550.4291-1.0913-0.201-0.60690.61560.23310.27780.70750.02350.9853-0.08589.744632.7782
210.87321.2164-0.1191.9257-0.97872.87690.2497-0.18550.22940.7336-0.09180.6821-0.1588-0.5424-0.12130.90750.06230.46460.6304-0.02770.9393.1465-1.56244.3045
221.9172-0.3606-0.02561.25510.87923.71970.1399-0.29260.08120.99340.00120.46090.1811-0.16210.00170.8533-0.00760.21750.4681-0.0130.45717.368-10.059946.7585
234.3722-0.572-0.97884.8414-0.96267.23720.1155-0.0595-0.0795-0.0186-0.24670.12440.2873-0.03840.04670.45220.01450.14070.4301-0.04640.396520.1572-14.867530.6501
243.2397-1.2878-0.4656.132-0.45264.13990.2460.42390.0997-0.7066-0.1290.1988-0.0251-0.3583-0.11020.43470.03830.02970.45150.04080.459712.9733-6.188320.0868
255.191-0.22580.37514.39981.21296.4670.35570.0142-0.36950.3169-0.34061.15450.0752-0.4988-0.05110.58050.11450.20720.5831-0.03830.80463.33461.060829.4228
264.041-0.62531.02323.31660.82123.72660.1065-0.30410.92740.2449-0.07540.6278-0.8612-0.8135-0.01350.64240.17770.17560.57340.09570.65817.28887.168929.6299
272.7712-0.56751.53333.24591.5095.70290.0733-0.14250.06630.7419-0.1710.041-0.4135-0.27830.00410.7478-0.15070.22130.50990.07690.484727.897114.138127.2851
285.67455.4613-0.44976.166-3.46129.9188-0.7656-0.06690.0727-0.77230.8879-0.47910.9656-0.6857-0.43020.69170.02-0.11940.8138-0.05190.95721.07732.07576.3896
293.4825-0.0957-0.58391.0125-1.99074.56650.1931-0.02120.05420.2773-0.1719-0.1758-0.2822-0.0193-0.11290.7655-0.07510.17310.4260.01630.587225.17169.558733.3267
304.181-0.90270.81251.5285-0.79670.4254-0.1639-0.89110.03880.63390.4205-0.17610.71150.576-0.06751.29390.0345-0.421.02980.16620.5389-21.350518.382863.9724
311.73490.33820.59071.52060.36012.7437-0.0090.00840.24251.11020.0205-0.4440.01130.63290.01921.1125-0.155-0.32671.00530.05220.4322-24.741226.968563.723
322.03030.02280.79792.3981.55061.6501-0.116-0.39240.1820.6891-0.02330.0613-0.29860.18220.12961.0012-0.0721-0.0630.66990.01830.3548-38.719130.33255.443
333.1405-1.00961.00483.8463-0.6573.83850.0885-0.1877-0.3620.89880.09530.58090.3161-0.1563-0.07260.7985-0.0308-0.00690.42750.10650.3482-41.810714.56947.7858
343.3233-0.08260.56761.3186-1.21741.06440.3831-0.4893-0.78670.8473-0.2050.26461.16410.53480.04511.44480.0563-0.08040.6160.17410.7503-39.94140.355152.922
351.82070.3058-0.14530.19220.55362.1777-0.2369-0.3652-0.61161.7681-0.248-0.98010.68040.87930.09761.42430.0847-0.40280.82540.28560.4168-27.1657.477156.7986
362.9519-0.1977-0.56390.1671-0.27952.28430.1123-0.596-1.05521.2662-0.2322-0.1770.62420.3337-0.07461.50120.0587-0.26731.14470.34920.656-27.89969.605767.4783
377.5706-1.62830.39852.613-2.69373.1919-0.6078-0.8224-2.04780.05250.11990.11240.1779-0.7090.23822.0694-0.30440.3340.85310.31981.006-47.70061.209471.6874
381.2355-1.26640.89155.6059-4.46083.7733-0.2995-1.0412-0.73420.2866-0.30620.43190.75570.62770.80081.6836-0.02340.11051.02140.15380.7089-48.27346.619368.9911
396.794-0.9124-0.62093.5579-2.98226.6322-0.5952-1.27630.27392.26850.57670.23770.1770.72380.231.59580.0450.05521.05890.1410.5599-42.669819.534275.4788
400.2137-0.97510.20594.6143-1.45842.7817-0.1097-0.3727-0.52340.12160.60751.23680.3222-0.15620.2621.7174-0.16580.86740.65930.35780.93443.783552.136253.0377
417.0673-2.48846.762.4335-1.36877.1328-0.06551.82781.5273-0.29850.19170.3412-0.6903-0.9548-0.27260.54640.0841-0.22190.83320.26080.796110.1644-6.16411.0032
425.7562-1.69522.30798.06822.47533.11340.79-0.5353-0.75620.21720.29781.2162-0.71120.4331-1.26261.21080.07820.07640.74720.1760.9512-31.094-5.247351.7349
430.042-0.2586-0.42113.57432.52094.37580.00170.9951.6901-0.52970.01060.0048-1.16140.3697-0.18440.9448-0.21590.04640.66770.04930.7444-26.217745.235515.9534
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 25 through 41 )
2X-RAY DIFFRACTION2chain 'A' and (resid 42 through 105 )
3X-RAY DIFFRACTION3chain 'A' and (resid 106 through 196 )
4X-RAY DIFFRACTION4chain 'A' and (resid 197 through 258 )
5X-RAY DIFFRACTION5chain 'A' and (resid 259 through 292 )
6X-RAY DIFFRACTION6chain 'A' and (resid 293 through 310 )
7X-RAY DIFFRACTION7chain 'A' and (resid 311 through 365 )
8X-RAY DIFFRACTION8chain 'B' and (resid 158 through 172 )
9X-RAY DIFFRACTION9chain 'B' and (resid 173 through 186 )
10X-RAY DIFFRACTION10chain 'B' and (resid 187 through 213 )
11X-RAY DIFFRACTION11chain 'C' and (resid 25 through 69 )
12X-RAY DIFFRACTION12chain 'C' and (resid 70 through 125 )
13X-RAY DIFFRACTION13chain 'C' and (resid 126 through 208 )
14X-RAY DIFFRACTION14chain 'C' and (resid 209 through 235 )
15X-RAY DIFFRACTION15chain 'C' and (resid 236 through 310 )
16X-RAY DIFFRACTION16chain 'C' and (resid 311 through 365 )
17X-RAY DIFFRACTION17chain 'D' and (resid 158 through 162 )
18X-RAY DIFFRACTION18chain 'D' and (resid 163 through 199 )
19X-RAY DIFFRACTION19chain 'D' and (resid 200 through 213 )
20X-RAY DIFFRACTION20chain 'E' and (resid 25 through 41 )
21X-RAY DIFFRACTION21chain 'E' and (resid 42 through 105 )
22X-RAY DIFFRACTION22chain 'E' and (resid 106 through 187 )
23X-RAY DIFFRACTION23chain 'E' and (resid 188 through 235 )
24X-RAY DIFFRACTION24chain 'E' and (resid 236 through 310 )
25X-RAY DIFFRACTION25chain 'E' and (resid 311 through 334 )
26X-RAY DIFFRACTION26chain 'E' and (resid 335 through 365 )
27X-RAY DIFFRACTION27chain 'F' and (resid 158 through 169 )
28X-RAY DIFFRACTION28chain 'F' and (resid 170 through 183 )
29X-RAY DIFFRACTION29chain 'F' and (resid 184 through 213 )
30X-RAY DIFFRACTION30chain 'G' and (resid 26 through 61 )
31X-RAY DIFFRACTION31chain 'G' and (resid 62 through 105 )
32X-RAY DIFFRACTION32chain 'G' and (resid 106 through 187 )
33X-RAY DIFFRACTION33chain 'G' and (resid 188 through 244 )
34X-RAY DIFFRACTION34chain 'G' and (resid 245 through 275 )
35X-RAY DIFFRACTION35chain 'G' and (resid 276 through 334 )
36X-RAY DIFFRACTION36chain 'G' and (resid 335 through 366 )
37X-RAY DIFFRACTION37chain 'H' and (resid 158 through 183 )
38X-RAY DIFFRACTION38chain 'H' and (resid 184 through 199 )
39X-RAY DIFFRACTION39chain 'H' and (resid 200 through 213 )
40X-RAY DIFFRACTION40chain 'I' and (resid 53 through 61 )
41X-RAY DIFFRACTION41chain 'J' and (resid 54 through 61 )
42X-RAY DIFFRACTION42chain 'K' and (resid 53 through 61 )
43X-RAY DIFFRACTION43chain 'X' and (resid 53 through 61 )

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