PDB-7v5y: Crystal structure of hexameric complex of Sa2YoeB-Sa2YefM toxin-a...

ID or keywords:

Entry

Database: PDB / ID: 7v5y

Title

Crystal structure of hexameric complex of Sa2YoeB-Sa2YefM toxin-antitoxin from Staphylococcus aureus

Components

Antitoxin
Putative mRNA interferase YoeB

Keywords

ANTITOXIN / Toxin/antitoxin

Function / homology

Function and homology information

global gene silencing by mRNA cleavage / RNA catabolic process / endonuclease activity / negative regulation of DNA-templated transcription
Similarity search - Function

Biological species

Staphylococcus aureus (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.25 Å

Authors

Xue, L. / Khan, M.H. / Yue, J.

Funding support

China, 1items

Citation

Journal: J.Biol.Chem. / Year: 2022
Title: The two paralogous copies of the YoeB-YefM toxin-antitoxin module in Staphylococcus aureus differ in DNA binding and recognition patterns.
Authors: Xue, L. / Khan, M.H. / Yue, J. / Zhu, Z. / Niu, L.

History

Deposition	Aug 18, 2021	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Dec 15, 2021	Provider: repository / Type: Initial release
Revision 1.1	Feb 16, 2022	Group: Database references / Category: citation / Item: _citation.journal_volume / _citation.year
Revision 1.2	Nov 29, 2023	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	7v5y.cif.gz	246.2 KB	Display	PDBx/mmCIF format
PDB format	pdb7v5y.ent.gz	165.2 KB	Display	PDB format
PDBx/mmJSON format	7v5y.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	7v5y_validation.pdf.gz	466 KB	Display	wwPDB validaton report
Full document	7v5y_full_validation.pdf.gz	468.3 KB	Display
Data in XML	7v5y_validation.xml.gz	17.9 KB	Display
Data in CIF	7v5y_validation.cif.gz	25.6 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/v5/7v5y ftp://data.pdbj.org/pub/pdb/validation_reports/v5/7v5y	HTTPS FTP

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Related structure data

Related structure data	7v5zC 7v6wC 2a6qS S: Starting model for refinement C: citing same article (ref.)
Similar structure data	6nbl-1 6o03-1 4jwu-2 2c3w-d 2a6q-d 5jjq-3 3ply-d 5gxg-d 6nbl-2 5zz7-d Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

B: Antitoxin

D: Antitoxin

A: Antitoxin

C: Antitoxin

E: Putative mRNA interferase YoeB

F: Putative mRNA interferase YoeB

60.5 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software
Evidence: light scattering

Unit cell

Length a, b, c (Å)	51.989, 66.984, 82.361
Angle α, β, γ (deg.)	90.000, 105.210, 90.000
Int Tables number	4
Space group name H-M	P12₁1
Space group name Hall	P2yb
Symmetry operation	#1: x,y,z #2: -x,y+1/2,-z

#1: Protein	Antitoxin Mass: 9783.692 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (strain NCTC 8325 / PS 47) (bacteria) Strain: NCTC 8325 / PS 47 / Gene: SAOUHSC_02757 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q2FVF7
#2: Protein	Putative mRNA interferase YoeB / Toxin YoeB Mass: 10670.266 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (strain NCTC 8325 / PS 47) (bacteria) Strain: NCTC 8325 / PS 47 / Gene: SAOUHSC_02756 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q2FVF8
#3: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 127 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.29 Å³/Da / Density % sol: 46.25 %
Crystal grow	Temperature: 289.15 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.1 M Ammonium tartrate dibasic pH 7.0, 12% w/v Polyethylene glycol 3350

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: SSRF / Beamline: BL18U1 / Wavelength: 0.97791 Å
Detector	Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Mar 20, 2016
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.97791 Å / Relative weight: 1
Reflection	Resolution: 2.25→50 Å / Num. obs: 26095 / % possible obs: 99.9 % / Redundancy: 6.6 % / B_iso Wilson estimate: 40.07 Å² / CC_1/2: 0.999 / Net I/σ(I): 26.7
Reflection shell	Resolution: 2.25→2.29 Å / Num. unique obs: 1296 / CC_1/2: 0.845

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Processing

Software

Name	Version	Classification
PHENIX	1.15.2_3472	refinement
HKL-2000		data reduction
HKL-2000		data scaling
PHENIX		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 2A6Q

2a6q

Resolution: 2.25→48.56 Å / SU ML: 0.2272 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 23.8148
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2286	1225	4.7 %
R_work	0.1804	24848	-
obs	0.1827	26073	99.61 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 51.06 Å²

Refinement step

Cycle: LAST / Resolution: 2.25→48.56 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	3774	0	0	127	3901

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0038	3842
X-RAY DIFFRACTION	f_angle_d	0.5576	5196
X-RAY DIFFRACTION	f_chiral_restr	0.0462	576
X-RAY DIFFRACTION	f_plane_restr	0.0029	668
X-RAY DIFFRACTION	f_dihedral_angle_d	4.7092	2348

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.25-2.34	0.2754	139	0.2334	2653	X-RAY DIFFRACTION	97.42
2.34-2.44	0.2687	117	0.2268	2769	X-RAY DIFFRACTION	99.93
2.44-2.57	0.2872	154	0.2254	2780	X-RAY DIFFRACTION	99.86
2.57-2.73	0.2719	126	0.2175	2754	X-RAY DIFFRACTION	100
2.73-2.94	0.2842	141	0.2043	2751	X-RAY DIFFRACTION	99.9
2.94-3.24	0.2639	155	0.2001	2731	X-RAY DIFFRACTION	100
3.24-3.71	0.2125	149	0.1784	2770	X-RAY DIFFRACTION	99.86
3.71-4.67	0.2001	110	0.1487	2797	X-RAY DIFFRACTION	99.83
4.67-48.56	0.185	134	0.1599	2843	X-RAY DIFFRACTION	99.67

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.175377314623	0.0846535549671	-0.0433965670992	-0.107006445404	0.0836544745423	0.044071030851	0.049905521868	0.109677622364	-0.533789759454	0.257691143678	0.0254186982072	-0.152996814744	0.683205247086	0.482983727817	-0.000143564613836	0.460950955183	0.03566868516	-0.0279755830629	0.323055638607	0.00467865747503	0.353877165039	14.8463159141	-23.4038643413	30.095428491
2	0.591937796228	-0.424759403157	0.937514628859	2.10653014324	-0.0298974921442	1.25145142181	0.0793369437486	0.0200717432854	-0.140236315931	-0.417273337481	0.151055623629	0.107787091292	0.164905871722	0.0639068979254	0.000750048639379	0.322913546863	-0.0479413001249	0.00556149709874	0.300802089731	0.0334470943274	0.219273356293	8.97675311434	-14.087877443	22.1415986345
3	1.25967529204	-0.681902013731	-1.19224962708	0.654132324394	0.339485457069	0.417143328055	0.317426596949	0.343564548039	-0.23377837615	-0.19784911432	-0.201304777656	0.321429861217	-0.283164225328	-0.658855562114	-0.0566504452841	0.539640416735	0.0307534949306	-0.189231916757	0.516916081327	0.0643379230907	0.304058878163	-10.9181227125	1.6289255531	0.405841158907
4	0.571267913899	-0.249303668813	0.823729329272	0.690181635968	0.0823914932757	0.56126000842	-0.168748914851	-0.256114693998	0.629199407553	0.310759024603	0.229858847374	-0.421474128134	-0.264021610582	0.315773729443	0.0036604876902	0.474149133172	-0.0527547715198	0.0898053311888	0.412671789325	-0.0165193089353	0.49177099507	19.0979878616	17.8254932881	26.667547388
5	0.667648736069	-0.424066796728	-0.682512226823	0.423442864773	0.0380790111671	0.628005766561	0.24427732086	-0.114852897707	0.570080314847	-0.297028893349	-0.066347018409	0.552650634799	-0.0564666997688	0.664378814953	0.0634736171811	0.409698148546	-0.0673759880052	0.0268270081335	0.350495289822	0.0977757654044	0.346393244699	12.3290822005	18.2649887688	19.530022614
6	0.217160130301	-0.139267766194	-0.280308212116	0.0638978660618	0.0245578581243	-0.0939653913834	0.107844883178	-0.0871755196525	-0.0856561029092	0.284239719113	0.0858690215136	0.0580948207267	-0.129988377219	0.0227050853215	-4.56517307142E-5	0.302105641963	-0.00274158792155	0.047299387807	0.273680609068	0.111672663738	0.397267139115	0.431431719358	6.07497116533	26.284472967
7	1.5768673572	0.256622879645	0.994977784958	0.792035027922	1.04543598027	0.951985841872	0.0350014667681	-0.177340843792	0.369285826541	0.0693551193118	-0.152302277673	0.104186162263	0.0320377610834	-0.137658461969	1.66436425033E-5	0.162943010133	0.0336828833472	0.0262231617294	0.287159022298	0.0324090734351	0.398478264938	-19.7688703963	0.391942834063	33.5255985695
8	0.0497900780513	0.0387991034989	-0.00772713880058	0.205784885021	-0.227335599755	0.126402937615	0.127968451736	-0.218487266067	0.302567941976	0.233165924804	-0.0431880457009	-0.136322686695	-0.224891983829	0.370344844846	0.00113755763673	0.320118629152	-0.0188012838335	-0.0332336193419	0.347206097392	0.0603945836586	0.427981071122	17.8491445923	-7.4700189388	32.2628089672
9	0.186008567025	0.191368222123	0.0395851006845	0.311340278638	0.0543295213146	0.15733615046	0.30749620576	-0.521592782176	0.586306913111	-0.0775724587751	-0.235476986058	0.240136939383	0.538117876269	-0.24409484109	-0.000428275584842	0.301368826707	-0.00602982337762	0.0527522422132	0.403905858927	-0.00422088040217	0.311506858338	8.26327906285	-11.7559007749	36.2227799348
10	0.0213317268575	0.0188683461665	-0.00221494174504	0.0469494854586	-0.140598228228	0.0977398877948	0.385537152877	-0.309123164378	-0.135743368874	-0.399196063645	-0.0537139172814	0.416342296075	-0.0543722388684	-0.0852601591252	-0.000509743328476	0.266997417332	0.0185083935611	0.0243696429814	0.341866413794	0.0758973164374	0.373664903079	7.61300356504	-6.47872970865	28.1659632756
11	0.142571645286	-0.106642286662	0.114568525356	0.545336395225	-0.017837717662	0.0927063789698	-0.127128587822	0.238098189449	0.453316194069	-0.36070110435	0.0604262052381	-0.35286676967	0.201082049816	-0.199283322127	0.00152873418415	0.309131337259	-0.0167237418877	0.0740139964967	0.40775574871	0.0600646351168	0.416123201069	16.968780646	-8.77394267341	22.1046654486
12	0.0243553476072	-0.0965876186813	0.195530601014	0.200897321975	0.168850547242	0.0254698661228	0.126819712526	-0.0423772939649	0.124869226113	-0.171617541925	-0.34627823849	-0.0434887811985	0.405029441425	-0.27662827517	0.0012951614869	0.3347832277	-0.0784804698659	-0.0338032674239	0.371686177899	0.0453806832838	0.419135110917	-3.20864856009	-16.081065729	20.5880807945
13	0.24958148728	-0.224548087778	-0.17816714487	0.859477132952	0.0193446551095	0.511633412385	0.187972862529	0.154521066339	-0.405550299561	-0.588259111533	0.0750274337112	-0.18575139659	0.268037019987	0.312523617428	-0.000945214809628	0.455203135282	-0.0335519076916	0.0329797574061	0.393550576832	-0.0168472449433	0.320475165342	17.3954233337	7.44697713742	16.8746800021
14	0.297529784994	-0.196055895629	-0.132938283147	0.148120988683	0.164551359165	0.127349704657	-0.0968376708842	0.00175422247718	-0.855744371443	0.555557699143	0.610628484682	0.610781606269	-0.908819269311	0.198633926644	0.00997759592122	0.370163652202	-0.030930335974	-0.0322715467818	0.327320817184	0.094097640701	0.384745014622	16.2336434885	7.24759496106	28.4875169542
15	0.671662420506	-0.209676054797	0.0440065722243	-0.0591633685714	0.070843500222	0.64907407773	0.405274613295	0.12937630449	1.18877394869	-0.0890383976915	-0.166539209063	0.51191174309	0.00920513215608	0.0129727480383	0.0150036304271	0.34614243295	-0.0285760624723	-0.0206980529045	0.351481064678	0.164761641473	0.504404255762	-1.25594614802	16.1804439029	19.4159195238
16	-0.00708923612138	-0.0577255437943	-0.0356287545513	0.395305567072	-0.0394856642188	0.0350950754862	0.106636741142	0.353626346979	-0.185978253809	-0.564159384205	0.350130680805	0.416517741902	0.452886537451	0.371971780663	0.0161124970074	0.840188495676	0.100206196687	-0.0826153150342	0.776826750237	-0.050016553112	0.269471406173	-0.920331110336	-0.283715655589	-7.32720796902
17	0.707023373349	-0.0373992145511	-0.361574542645	0.00840238647409	0.0169809919343	0.206945443496	0.63925275777	0.461831017962	0.89832058754	-0.254441395553	-0.266524483571	0.760968829649	-0.576875147118	-0.512692716613	0.0354080547879	0.570574369418	0.0665348596925	0.0314128050808	0.423048092745	0.106770344772	0.466362045047	-9.77022450345	10.4594821424	4.6348447089
18	0.131947327027	0.00965964024002	0.311590133491	0.394230665124	0.0554900642863	0.328720690744	0.045672816294	0.130765565295	0.237219640472	-0.378016687791	0.094710512413	-0.443657014606	-0.517875630874	0.703295140155	0.0213174354758	0.733619466414	-0.0628745777934	0.0605942455381	0.389643688692	0.0743074317805	0.33900079774	2.8005784949	8.00422198131	-1.09305520258
19	0.593513057669	-0.562596456217	-0.322108098015	0.319224363354	0.162068188572	0.654409477777	0.175264088607	-0.155208655344	-0.274169051947	0.0477882359627	-0.106368265481	-0.227425223108	0.125377365934	0.118358124268	-4.70568206351E-5	0.530010539676	0.00793191677285	-0.0388322193619	0.36591308459	-0.00931103339878	0.37517531061	4.3086634677	-5.59735922642	5.24204050214
20	0.139643401797	0.0485079120572	-0.0168333916748	0.116706011481	-0.0350079813972	0.0287282466942	0.585387438968	-1.1918224803	-0.0437116645185	1.18450636765	0.711895040351	0.044201852861	-0.520455991876	0.791233486686	-0.00129307059474	0.49476889369	-0.0267374041913	-0.0378420804524	0.506949441478	-0.0355816300597	0.335870128706	-0.5753455651	7.18917778225	12.9489969625
21	0.498162754586	0.0486203744268	-0.187790463146	0.354730449387	-0.319095452092	0.587835206696	0.359946033142	0.175474250266	-0.180854327623	-0.279672547426	0.0545459607386	-0.170159473086	-0.0880823259168	-0.0841929634509	0.0089814128197	0.58642945074	0.0125339133985	-0.0769822076383	0.382464128246	-0.0613916259362	0.256429749592	-1.35431270925	-4.45694436264	-2.46358936188
22	0.00634540377479	-0.0668944714844	0.0216809528743	0.0279183690847	-0.0894382808713	0.0158322150742	0.247212986562	-0.529965098849	-0.24905749579	0.176222841448	-0.0808027365612	1.36809231261	0.0271139988511	-0.395398085985	-0.00374568952524	0.604474053253	-0.0874992696516	0.0544931202849	0.488169594094	0.0511544072843	0.48828405494	-10.5157036769	4.81326658967	12.79317906
23	-0.0483260626989	0.0671154331088	0.0916029606711	0.162597937257	-0.120975017655	0.038728470557	-0.329313140382	-0.0975440509	-0.142845956437	0.1200707015	0.0140246430124	0.419092102204	-0.376214245649	-0.00583641651434	0.000804324879331	0.298461591008	0.0132111669179	0.00426012215305	0.383821014638	-0.0804487114054	0.278076177047	-14.7079216098	1.52629761382	45.2001092781
24	0.121639347507	-0.00189921016637	-0.180817403859	0.0908575438935	-0.13278403161	0.358997147146	-0.141358773285	0.0737759363249	0.0532772549954	-0.138816833427	0.0902534408479	0.353396421407	0.16024606129	-0.165599344814	-1.23851249147E-5	0.261055241663	-0.0042782860844	-0.0117323798931	0.292369178275	-0.0110729227057	0.415914357222	-18.045512234	-8.73481606128	30.8633267278
25	0.255907611022	-0.169081734478	-0.150090967841	0.203705501025	0.114239058781	-0.0541373631451	0.0689769766496	-0.390516240794	-0.193747682245	0.195673429542	-0.00571712984993	-0.126572867867	0.158814920355	0.0961453988246	0.000250092649701	0.242296360333	-0.0181553428697	-0.00376183374369	0.39562720627	0.090976361742	0.366540209685	-8.81095308664	-6.6052736278	41.9760575913
26	0.289079361778	-0.0538976641742	-0.111073680808	0.0232387431356	0.330167244795	0.372865610687	-0.0483911468118	-0.00835752367544	0.455922228436	0.013649436407	-0.141463823653	0.0741622064129	-0.145833287658	0.266804749338	-0.000125982490828	0.227126488836	-0.0541857963545	-0.0334334935671	0.301724123355	-0.0153233367575	0.402630458656	-1.59356502462	4.00027085482	37.4019333213
27	0.521550371225	-0.161555696031	-0.182809609303	0.0483025858863	0.0708886350832	0.0559753375767	-0.238522871396	-1.26226245282	1.08727207433	-0.167507689821	0.323667100151	0.175279191975	-0.83625122275	0.317007076015	-0.0173753760692	0.329610886754	0.014339653813	0.0712238301328	0.272060048763	-0.0825750454116	0.724285746564	-6.95398072919	13.944294569	34.3759328178
28	0.64913978153	0.218937265897	0.217342228345	0.0303567714443	-0.128500855552	0.228060224677	0.0563028476838	-0.102851760528	0.0878441117814	-0.185453068386	-0.137310882295	0.0746829159177	-0.00386514950431	0.0195404320585	-0.000278561808962	0.225976564023	0.00406501926327	0.036289210705	0.318580138862	-0.0085011630182	0.354626009973	-7.11244013039	-0.316257761876	33.4426556017
29	0.135065201213	-0.0788303051127	0.0785410088852	0.15906202299	0.0708618730517	0.0307680797663	0.217155872216	-0.299162286077	0.184849522241	-0.113462129093	-0.22684871027	-0.257947183683	-0.0643137908162	-0.605873003042	-1.11373113911E-6	0.203758059033	-0.0131772989191	0.0302098707864	0.350954158455	-0.10840954344	0.382970949222	-13.8256701326	6.64695316164	43.6460342302
30	-0.0358910625018	0.0571783102981	0.0406720748465	0.0625657984526	-0.0103662728507	0.000416171857751	-0.127747566395	0.0821805577966	0.188703871665	-0.169494753735	0.0101743702521	0.299296574355	0.533728802647	0.319762824135	-0.00015507479833	0.294581178463	-0.0179112861643	-0.00602337081838	0.324439135738	0.0430588659546	0.266497235266	-14.1142942413	-4.43978304087	22.8980134579

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'B' and (resid 1 through 12 )
2	X-RAY DIFFRACTION	2	chain 'B' and (resid 13 through 57 )
3	X-RAY DIFFRACTION	3	chain 'B' and (resid 58 through 85 )
4	X-RAY DIFFRACTION	4	chain 'D' and (resid 1 through 23 )
5	X-RAY DIFFRACTION	5	chain 'D' and (resid 24 through 42 )
6	X-RAY DIFFRACTION	6	chain 'D' and (resid 43 through 57 )
7	X-RAY DIFFRACTION	7	chain 'D' and (resid 58 through 85 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 1 through 12 )
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 13 through 23 )
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 24 through 29 )
11	X-RAY DIFFRACTION	11	chain 'A' and (resid 30 through 42 )
12	X-RAY DIFFRACTION	12	chain 'A' and (resid 43 through 56 )
13	X-RAY DIFFRACTION	13	chain 'C' and (resid 1 through 29 )
14	X-RAY DIFFRACTION	14	chain 'C' and (resid 30 through 42 )
15	X-RAY DIFFRACTION	15	chain 'C' and (resid 43 through 56 )
16	X-RAY DIFFRACTION	16	chain 'E' and (resid 2 through 9 )
17	X-RAY DIFFRACTION	17	chain 'E' and (resid 10 through 24 )
18	X-RAY DIFFRACTION	18	chain 'E' and (resid 25 through 37 )
19	X-RAY DIFFRACTION	19	chain 'E' and (resid 38 through 60 )
20	X-RAY DIFFRACTION	20	chain 'E' and (resid 61 through 65 )
21	X-RAY DIFFRACTION	21	chain 'E' and (resid 66 through 82 )
22	X-RAY DIFFRACTION	22	chain 'E' and (resid 83 through 88 )
23	X-RAY DIFFRACTION	23	chain 'F' and (resid 2 through 9 )
24	X-RAY DIFFRACTION	24	chain 'F' and (resid 10 through 24 )
25	X-RAY DIFFRACTION	25	chain 'F' and (resid 25 through 38 )
26	X-RAY DIFFRACTION	26	chain 'F' and (resid 39 through 51 )
27	X-RAY DIFFRACTION	27	chain 'F' and (resid 52 through 56 )
28	X-RAY DIFFRACTION	28	chain 'F' and (resid 57 through 72 )
29	X-RAY DIFFRACTION	29	chain 'F' and (resid 73 through 82 )
30	X-RAY DIFFRACTION	30	chain 'F' and (resid 83 through 88 )

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