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- PDB-7sr5: Single chain trimer HLA-A*02:01 (Y108C, A163C) with Wilms tumor p... -

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Basic information

Entry
Database: PDB / ID: 7sr5
TitleSingle chain trimer HLA-A*02:01 (Y108C, A163C) with Wilms tumor protein peptide RMFPNAPYL
Components
  • VHH
  • Wilms tumor protein peptide,Beta-2-microglobulin,MHC class I antigen chimera
KeywordsIMMUNE SYSTEM / HLA / VHH / HPV / SCT
Function / homology
Function and homology information


posterior mesonephric tubule development / negative regulation of metanephric glomerular mesangial cell proliferation / positive regulation of metanephric ureteric bud development / thorax and anterior abdomen determination / metanephric epithelium development / regulation of animal organ formation / adrenal cortex formation / negative regulation of female gonad development / positive regulation of heart growth / visceral serous pericardium development ...posterior mesonephric tubule development / negative regulation of metanephric glomerular mesangial cell proliferation / positive regulation of metanephric ureteric bud development / thorax and anterior abdomen determination / metanephric epithelium development / regulation of animal organ formation / adrenal cortex formation / negative regulation of female gonad development / positive regulation of heart growth / visceral serous pericardium development / Nephron development / glomerular basement membrane development / diaphragm development / sex determination / mesenchymal to epithelial transition / metanephric mesenchyme development / metanephric S-shaped body morphogenesis / positive regulation of male gonad development / cellular response to gonadotropin stimulus / gonad development / Transcriptional regulation of testis differentiation / tissue development / podocyte differentiation / double-stranded methylated DNA binding / cardiac muscle cell fate commitment / hemi-methylated DNA-binding / male genitalia development / glomerulus development / camera-type eye development / C2H2 zinc finger domain binding / antigen processing and presentation of peptide antigen via MHC class I / ureteric bud development / branching involved in ureteric bud morphogenesis / negative regulation of gene expression via chromosomal CpG island methylation / adrenal gland development / germ cell development / antigen processing and presentation / vasculogenesis / cellular response to cAMP / epithelial cell differentiation / RNA splicing / kidney development / lumenal side of endoplasmic reticulum membrane / negative regulation of cell growth / ER to Golgi transport vesicle membrane / MHC class I protein complex / positive regulation of miRNA transcription / recycling endosome membrane / phagocytic vesicle membrane / male gonad development / heart development / early endosome membrane / DNA-binding transcription activator activity, RNA polymerase II-specific / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / negative regulation of translation / transcription cis-regulatory region binding / nuclear speck / immune response / positive regulation of apoptotic process / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of cell population proliferation / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / positive regulation of gene expression / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process / nucleolus / positive regulation of DNA-templated transcription / cell surface / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / RNA binding / zinc ion binding / extracellular region / nucleoplasm / nucleus / cytosol / cytoplasm
Similarity search - Function
Wilm's tumour protein, N-terminal / Wilm's tumour protein / Zinc finger, C2H2 type / zinc finger / Zinc finger C2H2 type domain profile. / MHC class I, alpha chain, C-terminal / Zinc finger C2H2 superfamily / MHC_I C-terminus / Zinc finger C2H2 type domain signature. / Zinc finger C2H2-type ...Wilm's tumour protein, N-terminal / Wilm's tumour protein / Zinc finger, C2H2 type / zinc finger / Zinc finger C2H2 type domain profile. / MHC class I, alpha chain, C-terminal / Zinc finger C2H2 superfamily / MHC_I C-terminus / Zinc finger C2H2 type domain signature. / Zinc finger C2H2-type / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / : / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
MHC class I antigen / Beta-2-microglobulin / Wilms tumor protein
Similarity search - Component
Biological speciesHomo sapiens (human)
Pongo pygmaeus (Bornean orangutan)
Lama glama (llama)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.35 Å
AuthorsFinton, K.A.K. / Rupert, P.B.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Cancer Institute (NIH/NCI) United States
CitationJournal: Front Immunol / Year: 2023
Title: Effects of HLA single chain trimer design on peptide presentation and stability.
Authors: Finton, K.A.K. / Rupert, P.B. / Friend, D.J. / Dinca, A. / Lovelace, E.S. / Buerger, M. / Rusnac, D.V. / Foote-McNabb, U. / Chour, W. / Heath, J.R. / Campbell, J.S. / Pierce, R.H. / Strong, R.K.
History
DepositionNov 7, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 23, 2022Provider: repository / Type: Initial release
Revision 1.1May 31, 2023Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Oct 25, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.3Nov 20, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Wilms tumor protein peptide,Beta-2-microglobulin,MHC class I antigen chimera
B: VHH
C: Wilms tumor protein peptide,Beta-2-microglobulin,MHC class I antigen chimera
D: VHH


Theoretical massNumber of molelcules
Total (without water)120,4414
Polymers120,4414
Non-polymers00
Water4,053225
1
A: Wilms tumor protein peptide,Beta-2-microglobulin,MHC class I antigen chimera
B: VHH


Theoretical massNumber of molelcules
Total (without water)60,2202
Polymers60,2202
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: Wilms tumor protein peptide,Beta-2-microglobulin,MHC class I antigen chimera
D: VHH


Theoretical massNumber of molelcules
Total (without water)60,2202
Polymers60,2202
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)117.476, 117.476, 261.269
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number92
Space group name H-MP41212
Space group name HallP4abw2nw
Symmetry operation#1: x,y,z
#2: -y+1/2,x+1/2,z+1/4
#3: y+1/2,-x+1/2,z+3/4
#4: x+1/2,-y+1/2,-z+3/4
#5: -x+1/2,y+1/2,-z+1/4
#6: -x,-y,z+1/2
#7: y,x,-z
#8: -y,-x,-z+1/2

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Components

#1: Protein Wilms tumor protein peptide,Beta-2-microglobulin,MHC class I antigen chimera / WT33


Mass: 47683.484 Da / Num. of mol.: 2 / Mutation: Y108C, A163C
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human), (gene. exp.) Pongo pygmaeus (Bornean orangutan)
Gene: WT1, B2M, HLA-A / Production host: Homo sapiens (human)
References: UniProt: P19544, UniProt: P16213, UniProt: A0A678ZGP6
#2: Antibody VHH


Mass: 12536.822 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Lama glama (llama) / Production host: Homo sapiens (human)
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 225 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.74 Å3/Da / Density % sol: 67.13 %
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / Details: 200 mM NH4Citrate, 14.5% PEG 3350

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Data collection

DiffractionMean temperature: 93 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 5.0.1 / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 17, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.35→50 Å / Num. obs: 76503 / % possible obs: 99.4 % / Redundancy: 7.1 % / Biso Wilson estimate: 40.97 Å2 / CC1/2: 0.997 / Net I/σ(I): 16.8
Reflection shellResolution: 2.35→2.43 Å / Num. unique obs: 7530 / CC1/2: 0.772

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Processing

SoftwareName: PHENIX / Version: 1.19.1_4122 / Classification: refinement
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7SR0

7sr0


Resolution: 2.35→48.74 Å / SU ML: 0.2797 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 24.0818
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2481 3825 5 %
Rwork0.2294 72678 -
obs0.2304 76503 99.42 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 49.08 Å2
Refinement stepCycle: LAST / Resolution: 2.35→48.74 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7279 0 0 225 7504
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00177464
X-RAY DIFFRACTIONf_angle_d0.478710155
X-RAY DIFFRACTIONf_chiral_restr0.03951077
X-RAY DIFFRACTIONf_plane_restr0.00421326
X-RAY DIFFRACTIONf_dihedral_angle_d4.84871051
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.35-2.380.30051490.29022603X-RAY DIFFRACTION99.1
2.38-2.410.31521440.28592661X-RAY DIFFRACTION100
2.41-2.440.30071470.27382678X-RAY DIFFRACTION99.96
2.44-2.480.30251470.26642638X-RAY DIFFRACTION99.71
2.48-2.520.27291320.26022656X-RAY DIFFRACTION99.68
2.52-2.550.26891340.25442687X-RAY DIFFRACTION100
2.55-2.60.2621440.24042661X-RAY DIFFRACTION100
2.6-2.640.26251320.25492659X-RAY DIFFRACTION99.96
2.64-2.690.3131470.26422672X-RAY DIFFRACTION99.96
2.69-2.740.31231340.26642683X-RAY DIFFRACTION99.89
2.74-2.80.30671370.26822680X-RAY DIFFRACTION99.89
2.8-2.860.291280.27122698X-RAY DIFFRACTION99.82
2.86-2.920.30421390.26542685X-RAY DIFFRACTION99.86
2.92-30.24761390.24532662X-RAY DIFFRACTION99.75
3-3.080.30241370.24682682X-RAY DIFFRACTION99.09
3.08-3.170.26581470.23882672X-RAY DIFFRACTION99.68
3.17-3.270.2581540.24532677X-RAY DIFFRACTION99.72
3.27-3.390.26631470.24592687X-RAY DIFFRACTION99.75
3.39-3.520.28521440.24512685X-RAY DIFFRACTION99.61
3.52-3.680.22691460.22092694X-RAY DIFFRACTION99.4
3.68-3.880.21191280.20722718X-RAY DIFFRACTION99.48
3.88-4.120.22451570.19832663X-RAY DIFFRACTION98.5
4.12-4.440.22041470.18212712X-RAY DIFFRACTION99
4.44-4.880.17441280.17182769X-RAY DIFFRACTION99.14
4.89-5.590.19961430.19722733X-RAY DIFFRACTION98.8
5.59-7.040.23191460.24182756X-RAY DIFFRACTION97.97
7.04-48.740.25991480.24732907X-RAY DIFFRACTION97.17
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.2628334336260.3936835192990.08216937666250.3579475090940.144593514494-0.0603760644844-0.239178865913-0.004307428069240.34861321441-0.0196983209288-0.05560075528820.07230307023920.00366537980770.0195807071459-0.005258267454280.183983357764-0.00360916044718-0.03654375476670.286591829475-0.04375704307670.326296002504-9.8269753141635.9566610207-29.1522997361
20.8278927653780.2019884159040.2429454654060.262005677382-0.1164924872180.104834809263-0.1979699351060.1515581674530.197033785122-0.007658361952970.1026449251320.04708008300820.07568423098680.111239547607-3.20545607673E-50.282922944243-0.0944648025792-0.0186604875630.3486475651550.02212043387890.260510071489-12.240449207127.628623743-46.7237754243
30.430074022119-0.182061886502-0.09357575593490.378561258880.101305199652-0.0782191837289-0.245662345966-0.007413191662840.660659940425-0.00623613619517-0.01307333614420.208679239876-0.05103194823080.0498709240834-0.004990344396270.298319773101-0.0913795556359-0.05797654097880.3540566383890.03045230601560.337660694415-13.202017115534.0029445941-39.6834849369
40.330394545151-0.2050229177650.1493480924820.3927948934450.09343350232640.170569950064-0.0825908367572-0.0238308212245-0.264654104804-0.279680030212-0.02072734050220.01107927128370.270066686905-0.0123377317024-2.15787905691E-50.32868775477-0.0249755857258-0.01995210494940.2849211728080.03017389795730.356964894015-0.62660284323328.0237202976-30.7565052903
50.6083134795940.5239689284490.6774763419310.8444447472850.1415994980640.761015292011-0.0187376795786-0.6455307232960.5090510738280.106389241327-0.2007657195020.04172843377640.0269404074933-0.137038444713-0.05041833037360.251104099856-0.00997037477661-0.02626099956940.492490195756-0.1791018447930.355614648431-5.5780529138340.7256142865-19.5513691345
60.195607260193-0.0867646873399-0.2247477189860.1140883412980.2417217935240.593504438772-0.2553177188620.07400884628240.364110232073-0.1324918058230.1511609757650.05810079101070.2769734842530.152123640427-0.01315766539930.307313069826-0.0727591998974-0.06553913223640.3059220254080.03002364434650.37573209062710.389565329642.1928646735-37.2353824037
70.212638536676-0.259133938705-0.2995351430050.2789395081820.2225922009080.15855392071-0.2118674723170.169196400480.573828293525-0.05339085522410.1624876195210.284377971764-0.2261749438250.0207860629977-0.001890322719720.418411875419-0.142595155775-0.2559418714220.3735874174820.2077649729690.670466641303-11.002999344249.0489323366-54.5434222661
80.07204406689210.0899511987373-0.03711025271020.1300537329830.0633926024310.0866483914001-0.228733984347-0.0783622890189-0.203208200160.113961010294-0.03363060450230.420971381713-0.02219708913870.0681610363977-0.009204126730290.3049559929690.01743393797350.0134978894520.3445245894790.05253079594660.37626357685-37.83208627779.07206594772-38.755584878
90.0370153313097-0.0369754167483-0.09231976600530.02006499721890.0448511984120.0482605063094-0.1710700146550.532482172408-0.325220174162-0.232415458650.0702030932565-0.00995435853509-0.09324130479990.0775087089759-3.91887031801E-50.397359922908-0.1527897628440.01851002443140.538202318361-0.03384869680420.379952834772-32.148420219410.1509175698-50.7683575545
100.4176127689910.278570121097-0.02946779650140.144114549349-0.0440169780868-0.00497526528525-0.133792410679-0.03067597777780.0822276693167-0.1124373746790.164556647413-0.1472206303240.0332186544757-0.0173745871448-2.11142317501E-50.288172435198-0.02015811868260.04973308354060.3095309232370.01646991391990.319925597244-25.95550122712.3663686899-41.1463634849
110.4590167697060.1915308624460.09636164067170.159715669407-0.1153348840930.169675445635-0.121066440701-0.0233647405546-0.05557936642470.04238313125520.09632690321720.1705883429930.0004652244669450.0564563635206-9.96314441056E-60.304315396492-0.006660771680970.02519291142690.28241805780.04112035172610.293921883774-32.109241640913.6639862928-40.8894591117
121.3396591675-0.681973576539-0.5874971333240.5470541351690.117711221714-0.149152387531-0.299109239125-0.309494665688-0.3557539059370.07525300478410.1761269854410.1294024912160.04433608764410.137096639009-0.0364858058820.345162096070.1364202434540.06585263575190.4073024505020.06225585715520.277325543664-13.957316255986.3735836685-23.9322383917
132.07828794884-1.44250731137-0.588442939380.8506604667820.03311523999750.842310720868-0.1898348734970.189326180848-0.2485046184610.07301734352710.06183741286430.139129823829-0.1985663787940.0139493765923-0.01761998591420.298542049720.03690255557090.02424232235310.292220417012-0.006516975600240.317284645263-4.169457917479.6834456517-39.5721166053
140.6367906293910.2752027847580.5207321860670.2846511186790.4223749216850.596915143462-1.22558891204-0.0840002543563-1.30178554354-0.06192652839850.4630207470380.3698849625010.3784084879851.02302628786-0.4644327777320.6292259486990.6426252985140.6366829721530.5593017673310.2773710327650.870281231036-7.8945339304169.4167143243-14.2298604493
150.00103474528039-0.02021405117810.001729889134490.0186374232745-0.01972270477380.02374000323390.287002768906-0.272757458091-0.027016529433-0.00692929586793-0.06770181103050.02205675788030.04912794638840.0215889975412-0.0001264745954450.3787311534420.02539692721070.03634762435310.4579819020360.06023842627030.298627841928-39.9752567815101.821615352-14.3584312315
160.00930893798886-0.00674293555443-0.02067292041130.01267625846910.01544677043130.01821993549720.1842019560570.3664317706320.379322991021-0.1184205042010.0123154564770.0997138257332-0.1623946065610.0471333820504-0.0001386024596260.302283180672-0.026317608958-0.04922282133760.4079457121050.1412625782390.464265567639-40.114208756113.134809057-33.7941392306
170.001283783756130.0100503857849-0.0113973851466-0.0282070450265-0.02818751792860.0202865019062-0.10266410378-0.5285909470.2738026379610.1576471249660.0883744449219-0.056143987643-0.133520551769-0.02849854506974.35511177861E-50.3530932912050.1087994788660.06440481417710.459563491640.04060570989540.395131776218-37.5623268827108.890468959-18.5733764851
180.2419742540640.218918269132-0.01988152538020.4148483120310.1910287932090.159926806638-0.130816095364-0.247102726698-0.109819641571-0.09029081353880.3191778831520.0981364377061-0.180700339429-0.218598386411-0.004824810881690.2790223037890.0783796910404-0.02741566726370.4285241914160.05149968320270.356944698519-31.553328697499.5063518347-19.0108410897
190.02990094482480.00889684905148-0.01382404814180.00450660900919-0.02022383647870.0217914441542-0.0917244038603-0.01503776770450.101078240623-0.0464710376702-0.106965882713-0.2693200011360.226878192784-0.0302638402278-0.0004611547832240.2742794776210.0349366479431-0.02905584613350.38078430295-0.03150287463470.30837054064-25.3492582162104.517988244-19.575314584
200.0382765101252-0.01453464435640.00192242936820.0150902932764-0.007167243466560.00217339859291-0.06056355248310.2674794375160.2636440115680.2009864891420.221366198516-0.0430494919840.1453797804630.0932232926954-6.56032762281E-50.2809363425420.0037102568919-0.02719607840420.452176094672-0.0204973417340.340903048187-23.8540233243107.397171646-28.5080852889
210.02163259112910.03039614935550.006815249351910.0406934700502-0.003717801015030.001159964945240.0536137154945-0.3195286241930.342665803605-0.1948099223130.120690988346-0.0409152891931-0.205127557189-0.09763662108110.0002720450754480.347756970670.0294585687593-0.01502131955130.420189873928-0.03769529846620.325099228338-29.9553390787111.516511577-16.5504530074
220.03907525946550.0350749819191-0.0166579753970.0217970622474-0.01157704766210.000438579709687-0.1155751166150.02674891402850.4951752582920.115904284222-0.00284315043095-0.02475062846080.0535960371112-0.009858814219417.81018159869E-50.3117029025640.07316577894540.01172112340330.33229539002-0.03671929958970.450674895575-33.4358869316110.874851722-19.3575625197
230.0461100471879-0.002703469061590.006745960398810.0104250677795-0.01452446300390.0129302525534-0.3766544494670.351541514477-0.06833190899320.01579481615010.0234046357254-0.1641019837570.216139989414-0.03507483362530.0003258668339470.3720774512860.0187988906903-0.02352027411770.3502814991160.03578027223050.351632319784-33.3845951927108.965594731-36.0641845721
240.1337413830750.0589627010625-0.06185963656910.216282429750.01617504084250.045566578183-0.332822513934-0.410640899766-0.165067815186-0.08826382682850.3010340943530.2465417163190.0778466658625-0.1924234712530.0001237512892670.3547954456650.1176283534740.01005190930910.3978398445490.06217871191020.219134917706-32.957750836197.4493464802-17.1897913988
25-0.005972506269790.0126148020720.01434178451920.00907708053877-0.01243294137090.0254723706568-0.2250768528940.4146313903680.0483664900912-0.009625075185530.407060619259-0.06340581001160.2191341493460.1777767471599.96827016523E-50.297258163438-0.0215296606433-0.03810630412890.4059644848180.07448172577560.336237746863-40.9710049366106.023922003-32.8803977565
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 35 )AA1 - 351 - 23
22chain 'A' and (resid 36 through 107 )AA36 - 10724 - 95
33chain 'A' and (resid 108 through 155 )AA108 - 15596 - 121
44chain 'A' and (resid 156 through 199 )AA156 - 199122 - 165
55chain 'A' and (resid 200 through 305 )AA200 - 305166 - 271
66chain 'A' and (resid 306 through 328 )AA306 - 328272 - 294
77chain 'A' and (resid 329 through 417 )AA329 - 417295 - 379
88chain 'B' and (resid 3 through 19 )BB3 - 191 - 17
99chain 'B' and (resid 20 through 35 )BB20 - 3518 - 33
1010chain 'B' and (resid 36 through 77 )BB36 - 7734 - 73
1111chain 'B' and (resid 78 through 117 )BB78 - 11774 - 113
1212chain 'C' and (resid 1 through 155 )CC1 - 1551 - 121
1313chain 'C' and (resid 156 through 349 )CC156 - 349122 - 306
1414chain 'C' and (resid 350 through 417 )CC350 - 417307 - 350
1515chain 'D' and (resid 3 through 9 )DD3 - 91 - 7
1616chain 'D' and (resid 10 through 19 )DD10 - 198 - 17
1717chain 'D' and (resid 20 through 27 )DD20 - 2718 - 25
1818chain 'D' and (resid 28 through 47 )DD28 - 4726 - 42
1919chain 'D' and (resid 48 through 58 )DD48 - 5843 - 53
2020chain 'D' and (resid 59 through 68 )DD59 - 6854 - 63
2121chain 'D' and (resid 69 through 77 )DD69 - 7764 - 72
2222chain 'D' and (resid 78 through 84 )DD78 - 8473 - 79
2323chain 'D' and (resid 85 through 92 )DD85 - 9280 - 87
2424chain 'D' and (resid 93 through 111 )DD93 - 11188 - 106
2525chain 'D' and (resid 112 through 117 )DD112 - 117107 - 112

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