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- PDB-7sq2: Reprocessed and refined structure of Phospholipase C-beta and Gq ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7sq2 | ||||||||||||||||||
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Title | Reprocessed and refined structure of Phospholipase C-beta and Gq signaling complex | ||||||||||||||||||
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Function / homology | ![]() Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion / Acetylcholine regulates insulin secretion / forebrain neuron development / PLC beta mediated events / regulation of melanocyte differentiation / Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / Acetylcholine regulates insulin secretion / Thromboxane signalling through TP receptor / Thrombin signalling through proteinase activated receptors (PARs) ...Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion / Acetylcholine regulates insulin secretion / forebrain neuron development / PLC beta mediated events / regulation of melanocyte differentiation / Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / Acetylcholine regulates insulin secretion / Thromboxane signalling through TP receptor / Thrombin signalling through proteinase activated receptors (PARs) / G-protein activation / G alpha (q) signalling events / PLC beta mediated events / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||||||||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | ||||||||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||||||||
![]() | Endo-Streeter, S.T. / Sondek, J. / Harden, T.K. | ||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Kinetic Scaffolding Mediated by a Phospholipase C-{beta} and Gq Signaling Complex Authors: Endo-Streeter, S.T. / Sondek, J. / Harden, T.K. | ||||||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 461.9 KB | Display | ![]() |
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PDB format | ![]() | 392 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 42.7 KB | Display | |
Data in CIF | ![]() | 59.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3ohm S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 38256.434 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 99865.281 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: Q01970, ![]() |
-Non-polymers , 6 types, 227 molecules 










#3: Chemical | ChemComp-ALF / | ||||||||
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#4: Chemical | #5: Chemical | ChemComp-GDP / | ![]() #6: Chemical | ChemComp-ACT / ![]() #7: Chemical | ChemComp-CA / | #8: Water | ChemComp-HOH / | ![]() |
-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.04 Å3/Da / Density % sol: 59.57 % |
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Crystal grow![]() | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 7% PEG 6000, 100 mM Hepes, 1 mM CaCl2, 100 mM magnesium acetate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jun 8, 2010 / Details: Rosenbaum-Rock vertical focusing mirror |
Radiation | Monochromator: Si 220. Rosenbaum-Rock double-crystal monochromator: liquid nitrogen cooled; sagittally focusing 2nd crystal, Rosenbaum-Rock vertical focusing mirror Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.6→40.55 Å / Num. obs: 48454 / % possible obs: 99.49 % / Redundancy: 5.6 % / CC1/2: 0.905 / CC star: 0.975 / Rpim(I) all: 0.035 / Rrim(I) all: 0.084 / Χ2: 1.804 / Net I/σ(I): 32.5 |
Reflection shell | Resolution: 2.6→2.666 Å / Redundancy: 5.5 % / Mean I/σ(I) obs: 2.67 / Num. unique obs: 2555 / CC1/2: 0.905 / CC star: 0.975 / Rpim(I) all: 0.304 / Rrim(I) all: 0.723 / Χ2: 0.957 / % possible all: 99.6 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 3OHM ![]() 3ohm Resolution: 2.6→40.55 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.925 / SU B: 29.23 / SU ML: 0.315 / Cross valid method: THROUGHOUT / ESU R: 0.444 / ESU R Free: 0.3 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 91.472 Å2
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Refinement step | Cycle: 1 / Resolution: 2.6→40.55 Å
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Refine LS restraints |
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