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- PDB-7re7: TCR mimic antibody (Fab fragment) in complex with AFP/HLA-A*02 -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 7re7
TitleTCR mimic antibody (Fab fragment) in complex with AFP/HLA-A*02
Components
  • Beta-2-microglobulin
  • Fab heavy chain
  • Fab light chain
  • MHC class I antigen
  • PHE-MET-ASN-LYS-PHE-ILE-TYR-GLU-ILE
KeywordsIMMUNE SYSTEM / TCR mimic antibody (Fab fragment) / class I Major Histocompatibility Complex (HLA-A*02) / alpha fetoprotein peptide.
Function / homology
Function and homology information


positive regulation of ferrous iron binding / positive regulation of transferrin receptor binding / positive regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / negative regulation of receptor binding / DAP12 interactions / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / cellular response to iron ion ...positive regulation of ferrous iron binding / positive regulation of transferrin receptor binding / positive regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / negative regulation of receptor binding / DAP12 interactions / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / cellular response to iron ion / Endosomal/Vacuolar pathway / lumenal side of endoplasmic reticulum membrane / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / cellular response to iron(III) ion / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / negative regulation of forebrain neuron differentiation / regulation of erythrocyte differentiation / peptide antigen assembly with MHC class I protein complex / ER to Golgi transport vesicle membrane / regulation of iron ion transport / response to molecule of bacterial origin / MHC class I peptide loading complex / HFE-transferrin receptor complex / T cell mediated cytotoxicity / antigen processing and presentation of endogenous peptide antigen via MHC class I / positive regulation of T cell cytokine production / MHC class I protein complex / multicellular organismal-level iron ion homeostasis / negative regulation of neurogenesis / positive regulation of receptor-mediated endocytosis / peptide antigen assembly with MHC class II protein complex / MHC class II protein complex / cellular response to nicotine / positive regulation of T cell mediated cytotoxicity / specific granule lumen / recycling endosome membrane / phagocytic vesicle membrane / peptide antigen binding / positive regulation of cellular senescence / negative regulation of epithelial cell proliferation / antigen processing and presentation of exogenous peptide antigen via MHC class II / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / Interferon gamma signaling / positive regulation of immune response / Modulation by Mtb of host immune system / positive regulation of T cell activation / sensory perception of smell / negative regulation of neuron projection development / positive regulation of protein binding / tertiary granule lumen / DAP12 signaling / MHC class II protein complex binding / late endosome membrane / iron ion transport / ER-Phagosome pathway / early endosome membrane / T cell differentiation in thymus / protein refolding / protein homotetramerization / intracellular iron ion homeostasis / amyloid fibril formation / learning or memory / immune response / Amyloid fiber formation / endoplasmic reticulum lumen / external side of plasma membrane / Golgi membrane / lysosomal membrane / signaling receptor binding / focal adhesion / Neutrophil degranulation / SARS-CoV-2 activates/modulates innate and adaptive immune responses / structural molecule activity / Golgi apparatus / endoplasmic reticulum / protein homodimerization activity / extracellular space / extracellular exosome / extracellular region / identical protein binding / membrane / metal ion binding / plasma membrane / cytosol
Similarity search - Function
MHC class I, alpha chain, C-terminal / MHC_I C-terminus / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / : / Beta-2-Microglobulin / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / : ...MHC class I, alpha chain, C-terminal / MHC_I C-terminus / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / : / Beta-2-Microglobulin / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
FORMIC ACID / TRIETHYLENE GLYCOL / Beta-2-microglobulin / MHC class I antigen
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.547 Å
AuthorsDasgupta, M. / Baker, B.M.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) United States
CitationJournal: Sci Rep / Year: 2022
Title: Validation and promise of a TCR mimic antibody for cancer immunotherapy of hepatocellular carcinoma.
Authors: Liu, C. / Liu, H. / Dasgupta, M. / Hellman, L.M. / Zhang, X. / Qu, K. / Xue, H. / Wang, Y. / Fan, F. / Chang, Q. / Yu, D. / Ge, L. / Zhang, Y. / Cui, Z. / Zhang, P. / Heller, B. / Zhang, H. ...Authors: Liu, C. / Liu, H. / Dasgupta, M. / Hellman, L.M. / Zhang, X. / Qu, K. / Xue, H. / Wang, Y. / Fan, F. / Chang, Q. / Yu, D. / Ge, L. / Zhang, Y. / Cui, Z. / Zhang, P. / Heller, B. / Zhang, H. / Shi, B. / Baker, B.M. / Liu, C.
History
DepositionJul 12, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 27, 2022Provider: repository / Type: Initial release
Revision 1.1Aug 3, 2022Group: Derived calculations / Category: pdbx_struct_assembly_gen / pdbx_struct_oper_list
Revision 1.2Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
I: Fab heavy chain
J: Fab light chain
H: Fab heavy chain
L: Fab light chain
A: MHC class I antigen
B: Beta-2-microglobulin
D: MHC class I antigen
E: Beta-2-microglobulin
C: PHE-MET-ASN-LYS-PHE-ILE-TYR-GLU-ILE
F: PHE-MET-ASN-LYS-PHE-ILE-TYR-GLU-ILE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)190,96629
Polymers187,96310
Non-polymers3,00319
Water2,864159
1
D: MHC class I antigen
E: Beta-2-microglobulin
F: PHE-MET-ASN-LYS-PHE-ILE-TYR-GLU-ILE
hetero molecules

I: Fab heavy chain
J: Fab light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)95,41115
Polymers93,9815
Non-polymers1,43010
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_554-x+1/2,-y,z-1/21
2
H: Fab heavy chain
L: Fab light chain
A: MHC class I antigen
B: Beta-2-microglobulin
C: PHE-MET-ASN-LYS-PHE-ILE-TYR-GLU-ILE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)95,55514
Polymers93,9815
Non-polymers1,5749
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)49.707, 200.912, 244.574
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121

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Components

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Protein , 2 types, 4 molecules ADBE

#3: Protein MHC class I antigen


Mass: 33950.453 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-A / Production host: Escherichia coli (E. coli) / References: UniProt: Q861F7
#4: Protein Beta-2-microglobulin


Mass: 11879.356 Da / Num. of mol.: 2 / Fragment: UNP residues 21-119
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P / Production host: Escherichia coli (E. coli) / References: UniProt: P61769

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Protein/peptide , 1 types, 2 molecules CF

#5: Protein/peptide PHE-MET-ASN-LYS-PHE-ILE-TYR-GLU-ILE


Mass: 1205.444 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)

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Antibody , 2 types, 4 molecules IHJL

#1: Antibody Fab heavy chain


Mass: 24068.963 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)
#2: Antibody Fab light chain


Mass: 22877.189 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)

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Non-polymers , 8 types, 178 molecules

#6: Chemical ChemComp-EPE / 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / HEPES


Mass: 238.305 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C8H18N2O4S / Comment: pH buffer*YM
#7: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C3H8O3
#8: Chemical
ChemComp-PG4 / TETRAETHYLENE GLYCOL


Mass: 194.226 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C8H18O5 / Comment: precipitant*YM
#9: Chemical ChemComp-P6G / HEXAETHYLENE GLYCOL / POLYETHYLENE GLYCOL PEG400


Mass: 282.331 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C12H26O7 / Comment: precipitant*YM
#10: Chemical ChemComp-PGE / TRIETHYLENE GLYCOL


Mass: 150.173 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C6H14O4
#11: Chemical ChemComp-1PE / PENTAETHYLENE GLYCOL / PEG400


Mass: 238.278 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H22O6 / Feature type: SUBJECT OF INVESTIGATION / Comment: precipitant*YM
#12: Chemical ChemComp-FMT / FORMIC ACID


Mass: 46.025 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: CH2O2
#13: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 159 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.25 Å3/Da / Density % sol: 62.14 %
Crystal growTemperature: 296.15 K / Method: vapor diffusion, hanging drop / pH: 7.2
Details: 18% v/v PEG 3350 and 0.2 M sodium formate solution, pH 7.2

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.97918 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Nov 25, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 2.54→50 Å / Num. obs: 81215 / % possible obs: 99.32 % / Redundancy: 12.4 % / CC1/2: 0.994 / Net I/σ(I): 19.9
Reflection shellResolution: 2.54→2.58 Å / Num. unique obs: 3257 / CC1/2: 0.764

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Processing

Software
NameVersionClassification
PHENIX1.16_3549refinement
PDB_EXTRACT3.27data extraction
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Starting model: 5 / Resolution: 2.547→49.201 Å / SU ML: 0.29 / Cross valid method: THROUGHOUT / σ(F): 0 / Phase error: 22.69 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2196 7854 9.97 %
Rwork0.1797 70900 -
obs0.1837 78754 96.43 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 193.38 Å2 / Biso mean: 52.0187 Å2 / Biso min: 17.99 Å2
Refinement stepCycle: final / Resolution: 2.547→49.201 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12743 0 224 159 13126
Biso mean--73.48 38.02 -
Num. residues----1619
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.547-2.57590.3541970.2819181575
2.5759-2.60620.29862400.2424217489
2.6062-2.6380.29572370.2332218291
2.638-2.67140.2982380.2199217391
2.6714-2.70660.28342390.2193224593
2.7066-2.74360.2862570.2449230194
2.7436-2.78280.31172560.2378231095
2.7828-2.82440.28022490.2298224395
2.8244-2.86850.29012600.2205232395
2.8685-2.91550.25882630.2123236396
2.9155-2.96580.27542550.2111228296
2.9658-3.01970.26942600.2188234897
3.0197-3.07780.26662630.2261238997
3.0778-3.14060.25922650.2192238298
3.1406-3.20890.27332610.2257234298
3.2089-3.28350.27942650.2268242198
3.2835-3.36560.28412690.2153239899
3.3656-3.45660.23432690.1955241099
3.4566-3.55830.22142700.1903246099
3.5583-3.67310.19452660.1782389100
3.6731-3.80430.2052750.16972467100
3.8043-3.95660.20392690.16162408100
3.9566-4.13650.20412760.14942484100
4.1365-4.35450.17942690.13582417100
4.3545-4.62710.15832750.12832482100
4.6271-4.98410.15162770.133248399
4.9841-5.48510.17072760.13832477100
5.4851-6.27740.19462800.15842533100
6.2774-7.90350.21922810.1722529100
7.9035-49.2010.16012970.152267099
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.7405-1.26961.37783.13470.05284.73320.24580.08090.1156-0.5388-0.15410.0747-0.42720.0551-0.08460.33170.05270.06350.4061-0.11170.3445-11.023414.647430.2692
20.78620.3661.50775.11542.083.37890.11690.130.0337-0.6911-0.2512-0.3083-1.2487-0.4371-0.06670.88190.1236-0.04920.3794-0.00730.4635-14.66269.4515-10.5628
30.5605-0.581-1.24565.86583.15054.548-0.17-0.0811-0.0375-0.1006-0.36660.5223-1.2118-0.90340.50110.64230.2017-0.1040.5012-0.02660.4162-19.57885.6077-7.857
44.80990.75642.92351.31352.87886.6763-0.05720.0702-0.03870.15390.2553-0.0115-2.07830.5428-0.2251.1103-0.02520.11070.9205-0.15270.452-13.17758.7668-26.1227
51.0196-0.0849-0.32811.22880.87623.04550.19360.2924-0.1011-0.2826-0.0147-0.1340.14810.2574-0.19030.41990.1293-0.00290.4723-0.1580.37215.0456-2.299326.1435
60.80620.2581.01360.65890.75986.1954-0.1150.22920.1316-0.43170.22890.0293-0.45960.6778-0.09720.5497-0.01340.04370.4377-0.10010.427-1.28486.36413.8599
72.7989-1.3499-0.21445.3118-1.24034.57790.1639-0.14460.61480.06230.0191-0.07-1.06050.5675-0.22030.7821-0.0844-0.01510.3596-0.10860.5293-3.19518.2595-2.4321
82.29170.4298-0.21970.9033-0.90290.96750.1610.57980.8596-1.33170.0991-0.4679-0.64070.5879-0.10981.5115-0.32290.19540.73460.11210.79760.692616.2269-14.2324
99.3567-2.36431.97479.7463-7.02958.7422-0.40390.34110.4638-1.014-0.6358-0.71640.06282.01950.540.9048-0.04430.12220.6853-0.03630.46573.73286.3744-12.6834
100.73650.3094-0.49820.90461.39634.51670.0343-0.23970.1356-0.0674-0.24130.1839-0.3418-0.44490.18850.34690.0717-0.03820.3741-0.05040.3263-21.1282-18.949417.1158
113.40551.2503-4.89632.41010.32849.37-1.0258-4.228-2.25313.1820.8268-0.59692.17671.14530.41091.9401-0.1443-0.24961.49790.49740.699-8.5141-27.604667.0883
124.71870.99120.64884.38811.02385.15530.2584-0.3404-0.56120.7007-0.0801-0.21160.573-0.2999-0.18810.4594-0.0775-0.05020.546-0.04640.3507-14.3643-16.603850.8693
130.71520.2738-0.3091.47750.54783.30580.0972-0.3089-0.17820.5365-0.0679-0.15610.3010.0362-0.01530.4575-0.0035-0.0320.2620.0180.2723-9.2386-37.201322.1403
147.2967-1.7093-0.85066.2299-0.53043.95480.5045-0.4497-1.19980.6307-0.09340.63021.5338-0.6709-0.26751.2025-0.3611-0.10260.70910.06840.6936-21.3669-32.164553.3084
151.71610.92540.22072.0572-0.15261.9399-0.0174-0.0227-0.1370.0819-0.0459-0.22780.06920.14580.08430.26790.05490.010.2710.0090.2345-11.587-35.9513-11.3619
161.02150.3988-0.8841.7342-1.32212.63870.0710.11690.10290.0885-0.01050.1497-0.1502-0.1121-0.08650.21320.0558-0.03390.2278-0.03980.2436-22.1272-26.3118-14.6905
172.87270.24971.62321.62411.10398.45620.18780.2911-0.321-0.4565-0.02430.18260.41070.0381-0.0570.4130.1086-0.04850.35440.07340.3797-11.6622-31.374-48.0135
183.2314-2.10862.38095.9433-6.57377.272-0.0417-0.27330.2776-0.528-0.2412-0.5598-0.1734-0.33910.24921.6631-0.0577-0.23440.64830.35671.2489-2.6913-29.5108-60.803
198.92915.8765-4.04274.5336-4.27666.71960.3156-0.27940.07480.242-0.26810.19210.415-0.1684-0.02640.4260.039-0.02260.2172-0.0340.3572-13.1675-45.6886-31.0077
206.238-6.2469-6.85436.25576.86817.53120.5219-0.07161.4706-1.1318-0.1501-1.8242-0.62491.8589-0.94990.44010.09520.23630.64220.12930.64727.3803-34.7582-40.559
212.51280.4864-2.45491.92670.69753.9573-0.1455-0.04190.15480.21260.1221-0.30620.73570.40450.04230.41030.1596-0.00290.40540.03750.2447-4.0888-45.1479-29.1756
222.4648-2.0054-3.42133.76672.44325.1064-0.016-0.03780.12590.31750.0929-0.46140.42560.516-0.10250.39380.1053-0.04760.35110.04920.2805-4.6807-43.3482-25.3111
237.4742-0.6491-5.15463.85280.3335.785-0.19960.15410.1359-0.1872-0.0082-0.81210.57310.78850.1430.3920.2301-0.00360.5690.10550.37433.0317-42.7261-33.8114
247.45163.7982-4.99284.8719-1.57044.3246-0.37050.0756-0.2934-0.1580.0821-0.26790.86110.15290.28050.48180.20490.01090.36970.04530.2944-4.6952-50.1229-36.1113
251.5510.1840.60440.9671-0.14021.61890.00740.03930.0153-0.03390.11950.22090.068-0.1477-0.06130.19380.0017-0.02160.46710.16370.369818.233-6.9704-66.5881
261.05270.2601-0.05520.79740.2053.1288-0.00260.11470.0477-0.09250.13470.07960.24020.1005-0.13540.20010.0722-0.07010.32190.17620.395722.0878-15.2959-59.1746
273.29950.0948-1.46062.6423-1.33856.263-0.0221-0.13070.0350.20980.12730.11180.4947-0.0441-0.12410.23010.0476-0.03230.30490.05140.338118.9025-9.6924-31.5723
282.34262.07031.99451.97011.59871.89050.42510.37150.49281.50590.49530.9272-0.8305-0.3113-0.86521.01890.1297-0.3780.87050.10381.093218.0718-0.9119-16.001
293.790.28060.36585.98090.83812.8155-0.1949-0.15540.30990.25150.4795-0.0018-0.2233-0.192-0.23520.18330.11930.04450.49940.19050.442810.67630.9037-45.2343
301.6090.62430.85583.28581.87723.4032-0.15480.03380.37810.23250.21460.3084-0.4106-0.464-0.01880.29320.1380.0570.55140.27270.58068.22444.0652-50.3621
312.28320.99261.4051.42390.58790.9660.0037-0.31890.23940.44190.24280.3051-0.1643-0.71420.32840.25520.18820.12580.79350.20450.64511.67371.7497-43.6227
327.96782.8868-3.13593.9068-3.11133.74430.07930.1774-0.35690.0061-0.0440.0520.1691-0.1863-0.0260.35040.032-0.06860.1471-0.04940.2429-19.2828-30.7795-4.477
333.49112.0288-0.93435.52260.21660.37750.2956-0.1619-0.18760.1894-0.06790.46710.63720.046-0.34740.2850.083-0.11120.40470.17580.348321.4136-13.1402-74.8294
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'I' and (resid 1 through 115 )I1 - 115
2X-RAY DIFFRACTION2chain 'I' and (resid 116 through 149 )I116 - 149
3X-RAY DIFFRACTION3chain 'I' and (resid 150 through 217 )I150 - 217
4X-RAY DIFFRACTION4chain 'I' and (resid 218 through 218 )I218
5X-RAY DIFFRACTION5chain 'J' and (resid 2 through 86 )J2 - 86
6X-RAY DIFFRACTION6chain 'J' and (resid 87 through 154 )J87 - 154
7X-RAY DIFFRACTION7chain 'J' and (resid 155 through 184 )J155 - 184
8X-RAY DIFFRACTION8chain 'J' and (resid 185 through 201 )J185 - 201
9X-RAY DIFFRACTION9chain 'J' and (resid 202 through 212 )J202 - 212
10X-RAY DIFFRACTION10chain 'H' and (resid 1 through 128 )H1 - 128
11X-RAY DIFFRACTION11chain 'H' and (resid 129 through 139 )H129 - 139
12X-RAY DIFFRACTION12chain 'H' and (resid 140 through 217 )H140 - 217
13X-RAY DIFFRACTION13chain 'L' and (resid 2 through 125 )L2 - 125
14X-RAY DIFFRACTION14chain 'L' and (resid 126 through 212 )L126 - 212
15X-RAY DIFFRACTION15chain 'A' and (resid 1 through 103 )A1 - 103
16X-RAY DIFFRACTION16chain 'A' and (resid 104 through 185 )A104 - 185
17X-RAY DIFFRACTION17chain 'A' and (resid 186 through 275 )A186 - 275
18X-RAY DIFFRACTION18chain 'A' and (resid 276 through 276 )A276
19X-RAY DIFFRACTION19chain 'B' and (resid 1 through 12 )B1 - 12
20X-RAY DIFFRACTION20chain 'B' and (resid 13 through 20 )B13 - 20
21X-RAY DIFFRACTION21chain 'B' and (resid 21 through 42 )B21 - 42
22X-RAY DIFFRACTION22chain 'B' and (resid 43 through 62 )B43 - 62
23X-RAY DIFFRACTION23chain 'B' and (resid 63 through 78 )B63 - 78
24X-RAY DIFFRACTION24chain 'B' and (resid 79 through 100 )B79 - 100
25X-RAY DIFFRACTION25chain 'D' and (resid 1 through 118 )D1 - 118
26X-RAY DIFFRACTION26chain 'D' and (resid 119 through 197 )D119 - 197
27X-RAY DIFFRACTION27chain 'D' and (resid 198 through 275 )D198 - 275
28X-RAY DIFFRACTION28chain 'D' and (resid 276 through 277 )D276 - 277
29X-RAY DIFFRACTION29chain 'E' and (resid 1 through 31 )E1 - 31
30X-RAY DIFFRACTION30chain 'E' and (resid 32 through 78 )E32 - 78
31X-RAY DIFFRACTION31chain 'E' and (resid 79 through 100 )E79 - 100
32X-RAY DIFFRACTION32chain 'C' and (resid 1 through 9 )C1 - 9
33X-RAY DIFFRACTION33chain 'F' and (resid 1 through 9 )F1 - 9

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