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- PDB-7re8: Class I MHC (HLA-A*02) presenting alpha fetoprotein peptide (AFP) -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 7re8
TitleClass I MHC (HLA-A*02) presenting alpha fetoprotein peptide (AFP)
Components
  • Beta-2-microglobulin
  • MHC class I antigen, A-2 alpha chain
  • PHE-MET-ASN-LYS-PHE-ILE-TYR-GLU-ILE
KeywordsIMMUNE SYSTEM / Class I Major Histocompatibility Complex (HLA-A*02) / Alpha fetoprotein peptide (AFP).
Function / homology
Function and homology information


negative regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / transferrin transport / cellular response to iron ion / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / peptide antigen assembly with MHC class II protein complex / cellular response to iron(III) ion ...negative regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / transferrin transport / cellular response to iron ion / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / peptide antigen assembly with MHC class II protein complex / cellular response to iron(III) ion / MHC class II protein complex / negative regulation of forebrain neuron differentiation / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / ER to Golgi transport vesicle membrane / peptide antigen assembly with MHC class I protein complex / regulation of erythrocyte differentiation / regulation of iron ion transport / HFE-transferrin receptor complex / response to molecule of bacterial origin / MHC class I peptide loading complex / T cell mediated cytotoxicity / positive regulation of T cell cytokine production / antigen processing and presentation of endogenous peptide antigen via MHC class I / antigen processing and presentation of exogenous peptide antigen via MHC class II / positive regulation of immune response / MHC class I protein complex / positive regulation of T cell activation / peptide antigen binding / positive regulation of receptor-mediated endocytosis / negative regulation of neurogenesis / cellular response to nicotine / positive regulation of T cell mediated cytotoxicity / multicellular organismal-level iron ion homeostasis / specific granule lumen / phagocytic vesicle membrane / recycling endosome membrane / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / Interferon gamma signaling / negative regulation of epithelial cell proliferation / MHC class II protein complex binding / Modulation by Mtb of host immune system / late endosome membrane / sensory perception of smell / positive regulation of cellular senescence / tertiary granule lumen / DAP12 signaling / T cell differentiation in thymus / negative regulation of neuron projection development / ER-Phagosome pathway / protein refolding / early endosome membrane / protein homotetramerization / amyloid fibril formation / intracellular iron ion homeostasis / learning or memory / endoplasmic reticulum lumen / Amyloid fiber formation / Golgi membrane / lysosomal membrane / external side of plasma membrane / focal adhesion / Neutrophil degranulation / SARS-CoV-2 activates/modulates innate and adaptive immune responses / structural molecule activity / endoplasmic reticulum / Golgi apparatus / protein homodimerization activity / extracellular space / extracellular exosome / extracellular region / identical protein binding / membrane / plasma membrane / cytosol
Similarity search - Function
MHC class I-like antigen recognition-like / Murine Class I Major Histocompatibility Complex, H2-DB; Chain A, domain 1 / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / : / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / : ...MHC class I-like antigen recognition-like / Murine Class I Major Histocompatibility Complex, H2-DB; Chain A, domain 1 / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / : / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold / Immunoglobulins / Immunoglobulin-like / Sandwich / 2-Layer Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
DI(HYDROXYETHYL)ETHER / TRIETHYLENE GLYCOL / MHC class I antigen / Beta-2-microglobulin
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.82 Å
AuthorsDasgupta, M. / Baker, B.M.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) United States
CitationJournal: Sci Rep / Year: 2022
Title: Validation and promise of a TCR mimic antibody for cancer immunotherapy of hepatocellular carcinoma.
Authors: Liu, C. / Liu, H. / Dasgupta, M. / Hellman, L.M. / Zhang, X. / Qu, K. / Xue, H. / Wang, Y. / Fan, F. / Chang, Q. / Yu, D. / Ge, L. / Zhang, Y. / Cui, Z. / Zhang, P. / Heller, B. / Zhang, H. ...Authors: Liu, C. / Liu, H. / Dasgupta, M. / Hellman, L.M. / Zhang, X. / Qu, K. / Xue, H. / Wang, Y. / Fan, F. / Chang, Q. / Yu, D. / Ge, L. / Zhang, Y. / Cui, Z. / Zhang, P. / Heller, B. / Zhang, H. / Shi, B. / Baker, B.M. / Liu, C.
History
DepositionJul 12, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 27, 2022Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.2Nov 13, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: MHC class I antigen, A-2 alpha chain
B: Beta-2-microglobulin
C: PHE-MET-ASN-LYS-PHE-ILE-TYR-GLU-ILE
F: PHE-MET-ASN-LYS-PHE-ILE-TYR-GLU-ILE
D: MHC class I antigen, A-2 alpha chain
E: Beta-2-microglobulin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)92,57924
Polymers90,1406
Non-polymers2,43918
Water1,04558
1
A: MHC class I antigen, A-2 alpha chain
B: Beta-2-microglobulin
C: PHE-MET-ASN-LYS-PHE-ILE-TYR-GLU-ILE
hetero molecules


Theoretical massNumber of molelcules
Total (without water)46,29012
Polymers45,0703
Non-polymers1,2199
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6930 Å2
ΔGint-3 kcal/mol
Surface area18920 Å2
MethodPISA
2
F: PHE-MET-ASN-LYS-PHE-ILE-TYR-GLU-ILE
D: MHC class I antigen, A-2 alpha chain
E: Beta-2-microglobulin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)46,29012
Polymers45,0703
Non-polymers1,2199
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7230 Å2
ΔGint2 kcal/mol
Surface area18770 Å2
MethodPISA
Unit cell
Length a, b, c (Å)63.207, 87.310, 78.724
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 2 through 276 or resid 301 through 302 or resid 304 through 305))
21(chain D and (resid 2 through 276 or resid 301 through 302 or resid 304 through 305))
12(chain B and (resid 1 through 100 or resid 203))
22(chain E and (resid 1 through 100 or resid 201))
13chain C
23chain F

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111(chain A and (resid 2 through 276 or resid 301 through 302 or resid 304 through 305))A2 - 276
121(chain A and (resid 2 through 276 or resid 301 through 302 or resid 304 through 305))A301 - 302
131(chain A and (resid 2 through 276 or resid 301 through 302 or resid 304 through 305))A304 - 305
211(chain D and (resid 2 through 276 or resid 301 through 302 or resid 304 through 305))D2 - 276
221(chain D and (resid 2 through 276 or resid 301 through 302 or resid 304 through 305))D301 - 302
231(chain D and (resid 2 through 276 or resid 301 through 302 or resid 304 through 305))D304 - 305
112(chain B and (resid 1 through 100 or resid 203))B0
212(chain E and (resid 1 through 100 or resid 201))E0
113chain CC1 - 9
213chain FF1 - 9

NCS ensembles :
ID
1
2
3

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Components

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Protein , 2 types, 4 molecules ADBE

#1: Protein MHC class I antigen, A-2 alpha chain


Mass: 31985.398 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-A / Production host: Escherichia coli (E. coli) / References: UniProt: A0A5B8RNS7
#2: Protein Beta-2-microglobulin


Mass: 11879.356 Da / Num. of mol.: 2 / Fragment: UNP residues 21-119
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P / Production host: Escherichia coli (E. coli) / References: UniProt: P61769

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Protein/peptide , 1 types, 2 molecules CF

#3: Protein/peptide PHE-MET-ASN-LYS-PHE-ILE-TYR-GLU-ILE


Mass: 1205.444 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)

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Non-polymers , 4 types, 76 molecules

#4: Chemical
ChemComp-PGE / TRIETHYLENE GLYCOL


Mass: 150.173 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C6H14O4
#5: Chemical
ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: C4H10O3
#6: Chemical ChemComp-1PE / PENTAETHYLENE GLYCOL / PEG400


Mass: 238.278 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H22O6 / Feature type: SUBJECT OF INVESTIGATION / Comment: precipitant*YM
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 58 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.41 Å3/Da / Density % sol: 48.96 %
Crystal growTemperature: 277.15 K / Method: vapor diffusion, hanging drop / pH: 6.5 / Details: 25 mM MES pH6.5, 150 mM NaCl, 25% v/v PEG 3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.97918 Å
DetectorType: DECTRIS EIGER2 X 16M / Detector: PIXEL / Date: Feb 23, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 2.82→58.48 Å / Num. obs: 20564 / % possible obs: 99 % / Redundancy: 6.5 % / CC1/2: 0.991 / Net I/σ(I): 8.4
Reflection shellResolution: 2.82→2.87 Å / Num. unique obs: 1016 / CC1/2: 0.918

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Processing

Software
NameVersionClassification
PHENIX1.17.1_3660refinement
PDB_EXTRACT3.27data extraction
PHENIXmodel building
DIALSdata scaling
PHASERphasing
DIALSdata reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Starting model: 5 / Resolution: 2.82→58.47 Å / SU ML: 0.33 / Cross valid method: THROUGHOUT / σ(F): 0 / Phase error: 24.02 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.239 2019 10 %
Rwork0.1855 18163 -
obs0.191 20182 97.2 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 100.1 Å2 / Biso mean: 29.6049 Å2 / Biso min: 0.73 Å2
Refinement stepCycle: final / Resolution: 2.82→58.47 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6338 0 162 58 6558
Biso mean--40.86 21.08 -
Num. residues----768
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A2703X-RAY DIFFRACTION9.627TORSIONAL
12D2703X-RAY DIFFRACTION9.627TORSIONAL
21B1007X-RAY DIFFRACTION9.627TORSIONAL
22E1007X-RAY DIFFRACTION9.627TORSIONAL
31C85X-RAY DIFFRACTION9.627TORSIONAL
32F85X-RAY DIFFRACTION9.627TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.82-2.890.27771410.23581234137594
2.89-2.970.27441370.2311252138994
2.97-3.060.31061410.23451254139596
3.06-3.150.28981450.2141280142595
3.15-3.270.28181420.2171277141996
3.27-3.40.29871410.21621275141698
3.4-3.550.28261350.20761258139395
3.55-3.740.21621530.18281340149399
3.74-3.970.24451430.17151312145599
3.98-4.280.20461460.15671308145499
4.28-4.710.19091500.142913231473100
4.71-5.390.1921500.143113451495100
5.4-6.790.21871460.18931333147998
6.79-58.470.21511490.18091372152199
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.45570.7685-0.2832.314-0.94693.33840.0411-0.0223-0.026-0.124-0.1353-0.096-0.27920.209-0.30880.17480.05380.0120.1653-0.04350.04222.77793.140133.1089
23.87851.10781.07614.74310.31193.28850.0926-0.06060.28980.0786-0.12220.3685-0.2269-0.4021-0.14490.16250.00840.03430.2571-0.01730.148616.90221.754543.0975
31.8387-0.0286-1.36840.7898-1.01432.3375-0.0820.1915-0.02580.09940.0138-0.295-0.2604-0.2467-0.39770.2518-0.03190.00180.2155-0.0440.120931.49243.671538.1645
41.6903-0.34940.07910.4662-0.29671.61130.06130.08390.0341-0.2731-0.20620.06030.0491-0.00830.11370.24370.06430.01190.1489-0.04730.079728.9293-5.838426.2596
53.94420.8481-2.40092.8654-1.37794.2537-0.1574-0.1924-0.4194-0.2088-0.094-0.20620.1790.49450.15610.19280.01390.03940.1645-0.02520.197938.1742-9.529829.3966
62.31332.0847-1.0483.56310.64523.4850.0377-0.0277-0.3050.552-0.1905-0.1285-0.2115-0.2248-0.25520.26240.0417-0.03040.0616-0.00580.136619.5707-13.794444.1741
75.3188-1.2192.06152.7122-1.34113.17470.024-0.0821-0.2421-0.0010.33050.5266-0.4302-0.3564-0.31230.18630.0283-0.00090.1033-0.02390.2842-5.2672-6.153826.742
81.4203-0.14250.49671.48960.08292.27680.1310.2468-0.0576-0.44060.0963-0.058-0.06270.00773.06170.141-0.04310.01510.20420.03680.0151-3.6038-7.473120.0358
92.94680.07821.27341.5215-0.37542.16910.08260.3738-0.6015-0.32940.0826-0.06870.38580.1297-0.20480.16740.0172-0.05850.209-0.04020.2441-5.1179-8.924417.6447
101.1397-0.4011-0.94840.81290.14381.4231-0.2707-0.1098-0.32890.16810.02240.10640.33840.2156-0.69350.28560.1250.05390.21650.10030.13613.3446.559824.6345
114.3649-1.1416-1.12461.1227-0.16362.0384-0.01050.0550.66840.13670.0172-0.1844-0.47180.028-0.5570.19590.01940.03010.2639-0.05540.15339.23110.488828.0255
122.75090.72060.14921.4629-0.60071.63420.3346-0.00820.46740.0992-0.21450.2999-0.5088-0.23270.18670.26280.08470.00860.1998-0.01820.16491.648112.737824.422
131.94220.10480.02831.59560.45842.49140.1671-0.11580.11950.0872-0.17910.13430.3022-0.1167-0.19970.2003-0.0156-0.00130.1296-0.01280.02558.7237-46.146618.5223
141.24260.096-0.23992.50650.20884.32780.4170.0672-0.08940.4312-0.1939-0.30950.3150.33020.20630.1639-0.0316-0.06250.1960.0210.161514.6358-44.674428.4815
154.29372.36154.06382.38933.2225.6232-0.0020.22640.20910.2993-0.07140.30950.58810.2754-0.12150.10860.0220.04180.17050.03730.1710.0274-46.641423.6183
162.6746-0.26370.90180.1412-0.08451.8646-0.08990.10690.2029-0.2814-0.06240.2075-0.1714-0.05330.03020.24790.0515-0.02090.16390.03560.08892.6105-37.290411.5173
174.15580.83932.41892.22542.45343.5761-0.0324-0.26340.4671-0.2772-0.21020.0897-0.4016-0.2920.31860.24810.04970.00620.17520.04710.2351-6.6782-33.558814.8248
184.2387-0.332-1.08073.8576-2.10721.5312-0.2746-0.37360.150.5163-0.0001-0.181-0.1972-0.0418-0.53280.18750.0641-0.00960.0907-0.06980.211112.0355-29.120229.2593
196.6754-1.8937-4.22861.65881.89435.50970.3033-0.09410.2324-0.20580.0816-0.2273-0.24960.0424-0.34930.23530.0089-0.02640.06980.02590.22436.7393-36.842412.1435
202.3050.2961-1.17281.75-0.31022.94260.03090.14220.1581-0.21990.02420.0733-0.03670.1655-0.07870.14070.00590.00350.17240.01870.111235.3895-35.82383.0237
210.63360.25790.29872.1655-1.91022.14240.16560.31820.99010.43550.27040.0148-0.7468-0.01250.11310.3927-0.12050.14250.2997-0.0080.635840.2272-27.46128.0455
220.3536-0.12690.03081.40010.79962.87710.1257-0.04090.09760.0010.0653-0.1728-0.07340.02011.13970.3096-0.00910.01650.3547-0.16360.197611.5768-51.33351.13
232.3863-0.69460.53321.39640.10561.8118-0.0981-0.1605-0.30550.2368-0.0477-0.07590.1498-0.0367-0.15990.11350.0270.00910.09060.02030.085928.3968-51.998313.0264
241.2078-0.2477-1.03331.25671.01671.42120.0055-0.2205-0.33450.54740.22060.14780.3059-0.10942.52090.360.0206-0.09340.21940.16760.271727.9804-61.372618.4834
253.03710.04110.86960.8920.23620.3494-0.2437-0.07290.20030.2672-0.05760.01110.07260.0752-0.53220.20450.04290.03250.10670.04630.146919.8974-48.237214.3382
260.9813-0.6439-0.11640.5761-0.25660.73740.3510.5814-0.5659-0.2887-0.0644-0.35110.32850.32720.13970.30510.0917-0.01740.211-0.08840.45429.4052-59.61477.7114
275.49542.19480.56146.49151.66553.10840.16360.70740.4422-0.55320.1967-0.40720.15760.2509-0.30930.2770.05780.06610.18110.040.188335.1783-53.29536.5254
285.64563.971.69035.4104-1.43593.14060.7347-0.3441-0.27950.4106-0.3057-0.48190.13250.32740.50190.33190.0380.06710.18660.03010.194433.9977-3.832937.7657
295.23382.0161.61865.33470.51185.19680.2153-0.67790.77150.4692-0.08540.6288-0.6754-0.6620.9190.28790.11970.04430.2951-0.06310.3304-2.5289-39.18823.2734
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 2 through 29 )A2 - 29
2X-RAY DIFFRACTION2chain 'A' and (resid 30 through 57 )A30 - 57
3X-RAY DIFFRACTION3chain 'A' and (resid 58 through 85 )A58 - 85
4X-RAY DIFFRACTION4chain 'A' and (resid 86 through 138 )A86 - 138
5X-RAY DIFFRACTION5chain 'A' and (resid 139 through 163 )A139 - 163
6X-RAY DIFFRACTION6chain 'A' and (resid 164 through 175 )A164 - 175
7X-RAY DIFFRACTION7chain 'A' and (resid 176 through 198 )A176 - 198
8X-RAY DIFFRACTION8chain 'A' and (resid 199 through 220 )A199 - 220
9X-RAY DIFFRACTION9chain 'A' and (resid 221 through 276 )A221 - 276
10X-RAY DIFFRACTION10chain 'B' and (resid 1 through 20 )B1 - 20
11X-RAY DIFFRACTION11chain 'B' and (resid 21 through 62 )B21 - 62
12X-RAY DIFFRACTION12chain 'B' and (resid 63 through 100 )B63 - 100
13X-RAY DIFFRACTION13chain 'D' and (resid 2 through 29 )D2 - 29
14X-RAY DIFFRACTION14chain 'D' and (resid 30 through 57 )D30 - 57
15X-RAY DIFFRACTION15chain 'D' and (resid 58 through 85 )D58 - 85
16X-RAY DIFFRACTION16chain 'D' and (resid 86 through 138 )D86 - 138
17X-RAY DIFFRACTION17chain 'D' and (resid 139 through 163 )D139 - 163
18X-RAY DIFFRACTION18chain 'D' and (resid 164 through 175 )D164 - 175
19X-RAY DIFFRACTION19chain 'D' and (resid 176 through 198 )D176 - 198
20X-RAY DIFFRACTION20chain 'D' and (resid 199 through 264 )D199 - 264
21X-RAY DIFFRACTION21chain 'D' and (resid 265 through 276 )D265 - 276
22X-RAY DIFFRACTION22chain 'E' and (resid 1 through 6 )E1 - 6
23X-RAY DIFFRACTION23chain 'E' and (resid 7 through 42 )E7 - 42
24X-RAY DIFFRACTION24chain 'E' and (resid 43 through 52 )E43 - 52
25X-RAY DIFFRACTION25chain 'E' and (resid 53 through 72 )E53 - 72
26X-RAY DIFFRACTION26chain 'E' and (resid 73 through 91 )E73 - 91
27X-RAY DIFFRACTION27chain 'E' and (resid 92 through 100 )E92 - 100
28X-RAY DIFFRACTION28chain 'C' and (resid 1 through 9 )C1 - 9
29X-RAY DIFFRACTION29chain 'F' and (resid 1 through 9 )F1 - 9

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