PDB-7qy6: Structure of E.coli Class 2 L-asparaginase EcAIII, wild type (WT ...

ID or keywords:

Entry

Database: PDB / ID: 7qy6

Title

Structure of E.coli Class 2 L-asparaginase EcAIII, wild type (WT EcAIII)

Components

Beta-aspartyl-peptidase
Isoaspartyl peptidase

Keywords

HYDROLASE / L-asparaginase / mutation

Function / homology

Function and homology information

beta-aspartyl-peptidase / asparagine catabolic process via L-aspartate / asparaginase activity / beta-aspartyl-peptidase activity / protein autoprocessing / hydrolase activity / cytoplasm
Similarity search - Function

Biological species

Escherichia coli (E. coli)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.65 Å

Authors

Loch, J.I. / Klonecka, A. / Kadziolka, K. / Bonarek, P. / Barciszewski, J. / Imiolczyk, B. / Brzezinski, K. / Jaskolski, M.

Funding support

Poland, 1items

Citation

Journal: Acta Crystallogr D Struct Biol / Year: 2022
Title: Structural and biophysical studies of new L-asparaginase variants: lessons from random mutagenesis of the prototypic Escherichia coli Ntn-amidohydrolase.
Authors: Loch, J.I. / Klonecka, A. / Kadziolka, K. / Bonarek, P. / Barciszewski, J. / Imiolczyk, B. / Brzezinski, K. / Gilski, M. / Jaskolski, M.

History

Deposition	Jan 27, 2022	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Jul 13, 2022	Provider: repository / Type: Initial release
Revision 1.1	Jan 31, 2024	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

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Validation report

Summary document	7qy6_validation.pdf.gz	398.6 KB	Display	wwPDB validaton report
Full document	7qy6_full_validation.pdf.gz	399.4 KB	Display
Data in XML	7qy6_validation.xml.gz	12.4 KB	Display
Data in CIF	7qy6_validation.cif.gz	20.8 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/qy/7qy6 ftp://data.pdbj.org/pub/pdb/validation_reports/qy/7qy6	HTTPS FTP

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Related structure data

Related structure data	7qq8C 7qsfC 7qtcC 7qvrC 7qymC 7qyxC 7r1gC 7r5cC 2zalS C: citing same article (ref.) S: Starting model for refinement
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search
Experimental dataset #1	Data reference: 10.18150/VAOJLJ / Data set type: diffraction image data

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#166 - Oct 2013 Proteasome similarity (1) #80 - Aug 2006 AAA+ Proteases similarity (1) #176 - Aug 2014 Dynein similarity (1) #60 - Dec 2004 Ubiquitin similarity (1)

Deposited unit

AAA: Isoaspartyl peptidase

BBB: Beta-aspartyl-peptidase

CCC: Isoaspartyl peptidase

DDD: Beta-aspartyl-peptidase

hetero molecules

67 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author
Evidence: gel filtration

Unit cell

Length a, b, c (Å)	50.478, 75.347, 147.991
Angle α, β, γ (deg.)	90.000, 90.000, 90.000
Int Tables number	19
Space group name H-M	P2₁2₁2₁

Noncrystallographic symmetry (NCS)

NCS domain:

ID	Ens-ID	Details
1	1	AAA
2	1	CCC
3	2	BBB
4	2	DDD

NCS domain segments:

Dom-ID	Component-ID	Ens-ID	Beg auth comp-ID	Beg label comp-ID	End auth comp-ID	End label comp-ID	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	1	ALA	ALA	ALA	ALA	AAA	A	4 - 155	4 - 155
2	2	1	ALA	ALA	ALA	ALA	CCC	C	4 - 155	4 - 155
3	3	2	THR	THR	GLU	GLU	BBB	B	179 - 313	1 - 135
4	4	2	THR	THR	GLU	GLU	DDD	D	179 - 313	1 - 135

NCS ensembles :

ID	Details
1	Local NCS retraints between domains: 1 2
2	Local NCS retraints between domains: 3 4

#1: Protein	Isoaspartyl peptidase / Beta-aspartyl-peptidase / EcAIII / Isoaspartyl dipeptidase Mass: 19013.773 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: iaaA, spt, ybiK, b0828, JW0812 / Plasmid: pET11d / Production host: Escherichia coli (E. coli) / Strain (production host): BLg 21 Gold / References: UniProt: P37595, beta-aspartyl-peptidase
#2: Protein	Beta-aspartyl-peptidase Mass: 14428.146 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: iaaA, HLZ50_00315 / Plasmid: pET11d / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 Gold / References: UniProt: A0A246NXR9, beta-aspartyl-peptidase
#3: Chemical	ChemComp-NA / SODIUM ION Mass: 22.990 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Na
#4: Chemical	ChemComp-CL / CHLORIDE ION Mass: 35.453 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Cl
#5: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 410 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.1 Å³/Da / Density % sol: 41.54 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 / Details: 20% PEG 6000, 0.2 M MgCl2 in 0.1M Tris-HCl pH 8.5

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P13 (MX1) / Wavelength: 0.97 Å
Detector	Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 21, 2021
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.97 Å / Relative weight: 1
Reflection	Resolution: 1.55→75.35 Å / Num. obs: 83105 / % possible obs: 99.5 % / Redundancy: 12.2 % / CC_1/2: 0.997 / R_merge(I) obs: 0.167 / R_rim(I) all: 0.158 / Net I/σ(I): 9.36
Reflection shell	Resolution: 1.55→1.64 Å / R_merge(I) obs: 1.39 / Mean I/σ(I) obs: 1.62 / Num. unique obs: 13084 / CC_1/2: 0.904 / R_rim(I) all: 1.237 / % possible all: 98

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Processing

Software

Name	Version	Classification
REFMAC	5.8.0267	refinement
XDS		data reduction
XDS		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 2ZAL

2zal

Resolution: 1.65→67.236 Å / Cor.coef. F_o:F_c: 0.965 / Cor.coef. F_o:F_c free: 0.942 / WR_factor R_free: 0.235 / WR_factor R_work: 0.193 / SU B: 5.112 / SU ML: 0.085 / Average fsc free: 0.8974 / Average fsc work: 0.9105 / Cross valid method: FREE R-VALUE / ESU R: 0.098 / ESU R Free: 0.102
Details: Hydrogens have been added in their riding positions

	R_factor	Num. reflection	% reflection
R_free	0.2348	814	1.188 %
R_work	0.1926	67730	-
all	0.193	-	-
obs	-	68544	99.587 %

Solvent computation

Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT

Displacement parameters

B_iso mean: 28.294 Å²

	B_aniso -1	B_aniso -2	B_aniso -3
1-	4.036 Å²	0 Å²	0 Å²
2-	-	-0.969 Å²	0 Å²
3-	-	-	-3.067 Å²

Refinement step

Cycle: LAST / Resolution: 1.65→67.236 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	4209	0	4	410	4623

Refine LS restraints

Refine-ID	Type	Dev ideal	Dev ideal target	Number
X-RAY DIFFRACTION	r_bond_refined_d	0.012	0.013	4298
X-RAY DIFFRACTION	r_bond_other_d	0.003	0.015	4113
X-RAY DIFFRACTION	r_angle_refined_deg	1.694	1.63	5831
X-RAY DIFFRACTION	r_angle_other_deg	1.564	1.57	9441
X-RAY DIFFRACTION	r_dihedral_angle_1_deg	6.848	5	585
X-RAY DIFFRACTION	r_dihedral_angle_2_deg	28.418	22.178	202
X-RAY DIFFRACTION	r_dihedral_angle_3_deg	12.857	15	686
X-RAY DIFFRACTION	r_dihedral_angle_4_deg	15.917	15	29
X-RAY DIFFRACTION	r_chiral_restr	0.086	0.2	574
X-RAY DIFFRACTION	r_gen_planes_refined	0.009	0.02	5001
X-RAY DIFFRACTION	r_gen_planes_other	0.002	0.02	937
X-RAY DIFFRACTION	r_nbd_refined	0.21	0.2	938
X-RAY DIFFRACTION	r_symmetry_nbd_other	0.182	0.2	4171
X-RAY DIFFRACTION	r_nbtor_refined	0.166	0.2	2150
X-RAY DIFFRACTION	r_symmetry_nbtor_other	0.086	0.2	2110
X-RAY DIFFRACTION	r_xyhbond_nbd_refined	0.141	0.2	325
X-RAY DIFFRACTION	r_metal_ion_refined	0.112	0.2	7
X-RAY DIFFRACTION	r_symmetry_nbd_refined	0.174	0.2	13
X-RAY DIFFRACTION	r_nbd_other	0.211	0.2	39
X-RAY DIFFRACTION	r_symmetry_xyhbond_nbd_refined	0.159	0.2	24
X-RAY DIFFRACTION	r_mcbond_it	1.348	2.053	2322
X-RAY DIFFRACTION	r_mcbond_other	1.346	2.052	2321
X-RAY DIFFRACTION	r_mcangle_it	2.039	3.069	2898
X-RAY DIFFRACTION	r_mcangle_other	2.039	3.071	2899
X-RAY DIFFRACTION	r_scbond_it	1.605	2.337	1976
X-RAY DIFFRACTION	r_scbond_other	1.602	2.333	1974
X-RAY DIFFRACTION	r_scangle_it	2.538	3.416	2928
X-RAY DIFFRACTION	r_scangle_other	2.538	3.416	2928
X-RAY DIFFRACTION	r_lrange_it	5.274	26.856	4898
X-RAY DIFFRACTION	r_lrange_other	5.191	26.338	4824
X-RAY DIFFRACTION	r_ncsr_local_group_1	0.081	0.05	4515
X-RAY DIFFRACTION	r_ncsr_local_group_2	0.067	0.05	3793

Refine LS restraints NCS

Ens-ID	Dom-ID	Auth asym-ID	Refine-ID	Type	Rms dev position (Å)	Weight position
1	1	AAA	X-RAY DIFFRACTION	Local ncs	0.0813	0.05008
1	2	CCC	X-RAY DIFFRACTION	Local ncs	0.0813	0.05008
2	3	BBB	X-RAY DIFFRACTION	Local ncs	0.06653	0.0501
2	4	DDD	X-RAY DIFFRACTION	Local ncs	0.06653	0.0501

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.65-1.693	0.206	47	0.244	4981	X-RAY DIFFRACTION	99.9404
1.693-1.739	0.277	59	0.249	4862	X-RAY DIFFRACTION	99.9797
1.739-1.79	0.271	44	0.239	4688	X-RAY DIFFRACTION	99.9789
1.79-1.845	0.221	51	0.223	4567	X-RAY DIFFRACTION	99.9351
1.845-1.905	0.314	65	0.232	4425	X-RAY DIFFRACTION	99.8888
1.905-1.972	0.272	60	0.234	4270	X-RAY DIFFRACTION	99.2664
1.972-2.046	0.282	43	0.213	4120	X-RAY DIFFRACTION	99.7843
2.046-2.13	0.22	49	0.215	3981	X-RAY DIFFRACTION	99.3345
2.13-2.224	0.283	41	0.21	3821	X-RAY DIFFRACTION	99.1782
2.224-2.333	0.258	40	0.198	3658	X-RAY DIFFRACTION	99.1421
2.333-2.459	0.258	47	0.193	3475	X-RAY DIFFRACTION	99.2952
2.459-2.608	0.186	50	0.183	3301	X-RAY DIFFRACTION	99.2301
2.608-2.788	0.229	38	0.194	3098	X-RAY DIFFRACTION	98.8962
2.788-3.011	0.227	36	0.181	2906	X-RAY DIFFRACTION	99.3919
3.011-3.298	0.219	33	0.179	2694	X-RAY DIFFRACTION	99.7075
3.298-3.686	0.206	30	0.174	2464	X-RAY DIFFRACTION	99.9199
3.686-4.254	0.209	30	0.157	2178	X-RAY DIFFRACTION	99.4147
4.254-5.206	0.297	16	0.162	1862	X-RAY DIFFRACTION	99.5758
5.206-7.344	0.322	18	0.196	1489	X-RAY DIFFRACTION	100
7.344-67.236	0.185	17	0.211	879	X-RAY DIFFRACTION	99.5556

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.1626	-0.3725	-0.037	1.2155	0.2173	0.0716	0.0324	0.0564	0.0107	-0.224	0.0076	-0.0828	-0.0204	0.0422	-0.04	0.1582	-0.0293	0.0236	0.0802	-0.0068	0.0858	16.6547	4.9398	24.4714
2	2.1966	-1.1531	3.4901	1.9213	-0.7087	6.5212	0.188	-0.2885	-0.0329	0.1482	-0.0921	-0.0235	0.5324	-0.6803	-0.0959	0.1538	-0.1018	0.0295	0.0988	0.0091	0.0415	8.7635	-8.341	11.1388
3	0.4781	0.323	0.0432	0.4636	0.2284	0.417	0.0331	0.0415	0.0485	-0.0396	-0.0544	-0.0241	0.0241	-0.0888	0.0214	0.0633	-0.0004	0.0099	0.0583	0.0129	0.0943	7.7256	7.5024	22.4458
4	0.6713	0.1675	-0.0603	0.1682	-0.2839	0.6585	-0.0656	0.0221	0.0363	-0.0035	0.0692	-0.0156	-0.0028	-0.0875	-0.0036	0.0344	0.0227	-0.004	0.0672	-0.0075	0.1262	1.9174	9.6566	38.7215
5	0.2241	0.4646	-0.5051	4.7399	2.5283	4.5448	-0.0089	0.1116	0.0271	0.0371	0.0196	0.0669	0.0608	-0.4317	-0.0107	0.0073	-0.001	-0.0211	0.1187	0.0292	0.0957	-8.071	8.0487	30.5301
6	0.5752	-1.0523	0.4264	5.4996	1.4897	1.7597	0.2421	0.0521	-0.0234	-0.556	-0.3704	0.2664	0.1249	-0.1461	0.1284	0.2027	-0.0416	0.0142	0.0797	-0.0421	0.0331	-4.9514	-9.5558	22.2295
7	3.2835	2.69	0.5995	2.3397	0.7649	0.7387	0.0639	-0.0469	-0.0284	0.1052	-0.0457	-0.0449	0.1051	0.0453	-0.0182	0.0514	-0.0041	-0.0021	0.0581	0.0205	0.1124	14.4665	8.5494	30.8303
8	0.382	0.243	-0.1855	0.1593	-0.1577	0.5741	-0.0542	0.0092	0.0779	-0.049	0.0039	0.0313	0.2093	0.0721	0.0504	0.079	0.0249	0.0102	0.0372	0.004	0.1196	14.5385	-1.4562	38.4839
9	1.2112	0.6024	-0.1651	0.3985	-0.2612	0.3472	-0.0395	0.0869	0.0294	-0.1263	-0.034	-0.0272	0.1952	0.1378	0.0735	0.1118	0.0839	0.0418	0.0782	0.0215	0.0878	23.4966	-5.1209	33.4114
10	2.3878	3.6037	0.6625	6.2762	1.562	0.6538	-0.0181	-0.0157	0.0051	-0.0434	0.011	-0.0286	0.0576	0.1167	0.0071	0.0785	0.0654	0.0359	0.1112	0.0204	0.0827	22.5341	-0.308	26.7824
11	0.4674	0.1752	-0.7292	2.8021	0.2091	1.245	0.0601	0.0291	-0.0468	-0.0255	-0.0877	-0.2048	-0.0842	-0.0217	0.0276	0.0662	0.0326	0.0242	0.0988	0.0003	0.0913	19.5969	5.6512	18.7056
12	3.392	-3.8504	2.0911	5.9566	-5.2605	6.559	-0.2988	-0.2092	0.0896	0.1588	0.1027	-0.1615	0.1741	0.1194	0.1961	0.1222	0.0445	0.0382	0.0313	-0.0102	0.0698	18.3843	-7.2873	17.0472
13	0.0174	-0.0642	-0.1502	0.2756	0.6376	1.4765	-0.0219	-0.0262	0.0152	0.0602	0.0745	-0.0185	0.1398	0.1882	-0.0526	0.0781	0.0544	-0.0004	0.0944	0.006	0.0989	16.0168	-5.8379	66.0043
14	3.7075	1.225	-3.9331	2.3268	0.9846	6.8969	0.3319	0.2365	-0.0534	-0.1395	-0.3548	-0.058	-0.6885	-0.7625	0.0229	0.1804	0.0961	-0.0569	0.1202	0.0053	0.0413	8.0874	8.9155	78.7628
15	0.0874	0.0685	0.1151	0.0715	-0.0459	1.2842	-0.0051	-0.1134	0.0017	-0.0084	-0.0853	-0.0031	0.1012	-0.1927	0.0903	0.063	0.0112	0.0071	0.1518	-0.0043	0.0574	5.6795	-6.5354	66.9994
16	0.9012	-0.2639	0.0042	0.1108	0.0942	0.7918	-0.079	-0.0655	0.0209	0.0087	0.0402	-0.0133	0.1277	-0.0656	0.0388	0.0878	-0.0346	0.0211	0.0447	-0.0031	0.0988	0.7606	-9.4039	50.3788
17	0.7756	1.6136	1.9951	4.8594	3.2046	5.7935	-0.0932	-0.2129	0.0272	-0.0016	0.0478	0.1007	-0.2955	-0.8345	0.0454	0.0591	0.1027	0.0012	0.2398	-0.0056	0.0433	-8.5805	-1.0976	62.3093
18	1.1083	-0.0326	-2.7129	2.6732	1.8906	7.909	0.2439	0.0637	0.1598	0.0771	-0.1225	0.3381	-0.4593	-0.2556	-0.1214	0.2481	0.0936	-0.0149	0.1032	-0.1044	0.1743	-6.1263	13.6986	64.8502
19	1.911	-1.449	0.0772	1.5968	0.45	0.5358	-0.042	-0.0291	0.0626	-0.0179	0.0001	-0.0325	-0.0229	0.0017	0.042	0.074	0.0605	0.029	0.0503	0.0234	0.1028	12.5672	-7.6136	59.2345
20	0.1311	-0.1157	0.0749	0.1048	-0.0911	0.4538	-0.0428	-0.0019	0.001	0.0366	-0.0038	0.0074	-0.0247	0.0723	0.0467	0.0488	0.0374	-0.0007	0.0521	0.0107	0.1197	15.2917	1.2488	51.4596
21	3.013	-2.6126	0.8296	5.0379	-0.0248	0.4072	-0.1414	0.0102	0.1539	0.2256	0.1131	-0.1757	-0.0147	0.0578	0.0283	0.033	-0.0165	-0.0109	0.1354	0.0181	0.0643	26.4052	4.4626	57.7305
22	0.4852	-0.6978	0.1628	1.2387	-0.347	0.1596	-0.0189	-0.0804	0.0001	-0.0265	0.0085	0.0327	-0.001	0.0971	0.0104	0.0586	0.0107	-0.0199	0.1688	0.0186	0.0709	20.7864	2.0069	59.5207
23	0.6687	-0.5303	-0.1346	3.5982	0.459	0.2289	0.0534	-0.0298	0.0843	0.323	-0.1044	-0.0358	0.0013	0.1616	0.0509	0.1016	-0.0143	-0.0241	0.2051	0.0462	0.0461	16.9116	-2.7723	69.5967
24	0.01	-0.1753	0.0957	5.4729	-0.9558	1.1325	0.0007	0.0137	0.0029	0.1357	-0.0395	-0.0573	0.0122	0.2009	0.0387	0.0736	0.0236	-0.0298	0.0992	0.0117	0.0593	17.7243	-0.243	73.8943

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	ALL	AAA	3 - 14
2	X-RAY DIFFRACTION	2	ALL	AAA	15 - 24
3	X-RAY DIFFRACTION	3	ALL	AAA	25 - 77
4	X-RAY DIFFRACTION	4	ALL	AAA	78 - 133
5	X-RAY DIFFRACTION	5	ALL	AAA	134 - 147
6	X-RAY DIFFRACTION	6	ALL	AAA	148 - 156
7	X-RAY DIFFRACTION	7	ALL	BBB	179 - 200
8	X-RAY DIFFRACTION	8	ALL	BBB	201 - 253
9	X-RAY DIFFRACTION	9	ALL	BBB	254 - 281
10	X-RAY DIFFRACTION	10	ALL	BBB	282 - 294
11	X-RAY DIFFRACTION	11	ALL	BBB	295 - 309
12	X-RAY DIFFRACTION	12	ALL	BBB	310 - 313
13	X-RAY DIFFRACTION	13	ALL	CCC	3 - 14
14	X-RAY DIFFRACTION	14	ALL	CCC	15 - 24
15	X-RAY DIFFRACTION	15	ALL	CCC	25 - 78
16	X-RAY DIFFRACTION	16	ALL	CCC	79 - 136
17	X-RAY DIFFRACTION	17	ALL	CCC	137 - 149
18	X-RAY DIFFRACTION	18	ALL	CCC	150 - 157
19	X-RAY DIFFRACTION	19	ALL	DDD	179 - 201
20	X-RAY DIFFRACTION	20	ALL	DDD	202 - 255
21	X-RAY DIFFRACTION	21	ALL	DDD	256 - 266
22	X-RAY DIFFRACTION	22	ALL	DDD	267 - 292
23	X-RAY DIFFRACTION	23	ALL	DDD	293 - 303
24	X-RAY DIFFRACTION	24	ALL	DDD	304 - 313

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