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- PDB-7qq8: Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-8 ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7qq8 | ||||||
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Title | Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-8 (G206Y, R207Q, D210P, S211T) | ||||||
![]() | (Beta-aspartyl-peptidase) x 2 | ||||||
![]() | HYDROLASE / L-asparaginase / Ntn-hydrolase / EcAIII | ||||||
Function / homology | beta-aspartyl-peptidase / Peptidase T2, asparaginase 2 / Asparaginase / asparaginase / asparaginase activity / beta-aspartyl-peptidase activity / Nucleophile aminohydrolases, N-terminal / : / Beta-aspartyl-peptidase![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Loch, J.I. / Kadziolka, K. / Jaskolski, M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and biophysical studies of new L-asparaginase variants: lessons from random mutagenesis of the prototypic Escherichia coli Ntn-amidohydrolase. Authors: Loch, J.I. / Klonecka, A. / Kadziolka, K. / Bonarek, P. / Barciszewski, J. / Imiolczyk, B. / Brzezinski, K. / Gilski, M. / Jaskolski, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 222.6 KB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.1 KB | Display | ![]() |
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Full document | ![]() | 453.5 KB | Display | |
Data in XML | ![]() | 26 KB | Display | |
Data in CIF | ![]() | 38 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7qsfC ![]() 7qtcC ![]() 7qvrC ![]() 7qy6C ![]() 7qymC ![]() 7qyxC ![]() 7r1gC ![]() 7r5cC ![]() 2zalS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
Experimental dataset #1 | Data reference: ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
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Components
#1: Protein | Mass: 19013.773 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 14501.256 Da / Num. of mol.: 2 / Mutation: G206Y, R207Q, D210P, S211T Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: ybiK, iaaA, iaaA_1, A6519_003716, A8W81_003313, ABE90_014520, ACN002_0860, ACN68_01690, ACN81_14685, ACU57_05935, AM270_18905, AM464_21585, AML23_23425, AT335_000572, AUQ29_12475, BANRA_01341, ...Gene: ybiK, iaaA, iaaA_1, A6519_003716, A8W81_003313, ABE90_014520, ACN002_0860, ACN68_01690, ACN81_14685, ACU57_05935, AM270_18905, AM464_21585, AML23_23425, AT335_000572, AUQ29_12475, BANRA_01341, BANRA_01555, BANRA_01783, BANRA_04872, BB545_05440, BG944_002984, BHF52_24110, BHS81_05055, BJI68_11585, BK292_21235, BMT50_00465, BMT91_20430, BN17_06281, BO068_000728, BOH76_20420, BON70_18240, BON72_22520, BON73_01295, BON76_25520, BON93_04325, BON94_25670, BON95_17475, BON98_19810, BTQ06_08955, BUE81_13890, BvCms2454_00926, BvCmsHHP019_05523, BvCmsKKP057_04358, BvCmsKKP061_03116, BvCmsKSNP073_02388, BvCmsKSP011_05390, BvCmsNSNP036_00638, BvCmsSIP082_02366, BVL39_24345, C5F72_1CFS32920, C5N07_02820, C5Y87_11355, C6B22_17135, C6N50_003302, C9114_12655, C9160_09270, CA593_24960, CCS08_19790, CF32_004569, CN875_002453, CO706_26955, COD50_05845, CV83915_00751, D0X26_04985, D3O91_06915, D4V09_05900, D6T60_26500, D9D77_09725, D9H13_07905, D9H94_11600, DAH18_23535, DAH34_00665, DAH37_00135, DB282_07105, DEN89_02655, DEN95_11270, DEO15_17945, DIV22_10390, DM968_05545, DS732_09125, DTL43_10625, DXT69_18875, DXT71_08810, E0I42_09220, E2119_12510, E2127_00560, E2128_05045, E2129_10495, E4K51_02720, E4K54_01015, E5S34_19335, E5S35_03615, E5S43_04770, E5S47_20155, E5S52_10740, EAI46_07630, EAN77_08435, EAX79_16315, EC3234A_14c00240, EC95NR1_05101, EHD79_04065, EHH55_13640, EI021_00645, EI041_07210, EIA13_17230, EIZ93_05000, EKI52_06310, EL79_3055, EL80_3016, ELT21_01570, ELV08_05385, ELY05_10390, ETECE36_04109, ETECE925_03147, F9V24_09900, FC554_12230, FOI11_009250, FOI11_10795, FTV92_09805, FV293_02290, FVB16_25145, FWK02_18715, FY127_05135, G9448_06620, GF646_11140, GIB53_03275, GKF86_16910, GKF89_13745, GNZ05_09030, GP650_11285, GP662_09655, GP946_16590, GP979_11145, GQE64_04115, GQF58_22125, GQF59_19085, GQM04_07810, GQM06_12705, GQM09_17025, GQM10_22525, GQR15_10775, GRW05_01460, GRW57_08990, GRW81_11185, GUC01_03155, H4P47_16170, H4P50_17220, H4P51_17070, HCF72_001403, HHH44_001163, HIE44_001059, HIR12_003510, HKA49_002523, HmCms184_01733, HMV95_02640, HNC52_00665, HNV65_21255, HPE39_07700, HVV53_24715, HVW11_02200, HX136_17405, I6H00_09890, I6H01_21700, I6H02_22440, JE86ST02C_09390, JE86ST05C_09930, NCTC10090_00399, NCTC10418_05023, NCTC11181_00590, NCTC13216_03372, NCTC4450_03859, NCTC8008_02934, NCTC8500_03679, NCTC8622_06405, NCTC8960_00506, NCTC9036_03423, NCTC9045_03895, NCTC9073_02377, NCTC9111_03436, NCTC9703_02658, ND22_003961, PGD_02506, RG28_00360, SAMEA3472043_03316, SAMEA3472080_01956, SAMEA3484427_01111, SAMEA3484429_00220, SAMEA3751407_00331, SAMEA3752386_00245, SAMEA3753300_01714, WP2S18E08_31210, WP4S18E08_29890, WQ89_23460, WR15_25260 Plasmid: pET11d / Production host: ![]() ![]() References: UniProt: J7QNS8, beta-aspartyl-peptidase, asparaginase #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.43 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 20% PEG 4000; 15% PEG 400; 0,2 M MgCl2 in 0.1M Tris-HCl pH 8.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SEALED TUBE / Type: agilent / Wavelength: 1.5406 Å |
Detector | Type: RIGAKU HyPix-6000HE / Detector: PIXEL / Date: Jul 24, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5406 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→19.161 Å / Num. obs: 48975 / % possible obs: 93.6 % / Redundancy: 2.6 % / CC1/2: 0.996 / Rmerge(I) obs: 0.076 / Rrim(I) all: 0.092 / Net I/σ(I): 8 |
Reflection shell | Resolution: 1.8→1.84 Å / Rmerge(I) obs: 0.519 / Mean I/σ(I) obs: 1.6 / Num. unique obs: 2945 / CC1/2: 0.692 / Rrim(I) all: 0.631 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2ZAL Resolution: 1.8→19.161 Å / Cor.coef. Fo:Fc: 0.945 / Cor.coef. Fo:Fc free: 0.921 / SU B: 8.88 / SU ML: 0.117 / Cross valid method: FREE R-VALUE / ESU R: 0.145 / ESU R Free: 0.137
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 17.964 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→19.161 Å
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Refine LS restraints |
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Refine LS restraints NCS |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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