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- PDB-7ppc: Ternary signalling complex of BMP10 bound to ALK1 and BMPRII -

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Basic information

Entry
Database: PDB / ID: 7ppc
TitleTernary signalling complex of BMP10 bound to ALK1 and BMPRII
Components
  • Bone morphogenetic protein 10
  • Bone morphogenetic protein receptor type-2
  • Serine/threonine-protein kinase receptor R3
KeywordsSIGNALING PROTEIN / BMPRII BMP10 ALK1 Signalling complex
Function / homology
Function and homology information


semi-lunar valve development / negative regulation of chondrocyte proliferation / regulation of cardiac muscle hypertrophy in response to stress / regulation of lung blood pressure / lymphatic endothelial cell differentiation / pulmonary valve development / regulation of endothelial cell proliferation / positive regulation of cell proliferation involved in heart morphogenesis / dorsal aorta morphogenesis / chondrocyte development ...semi-lunar valve development / negative regulation of chondrocyte proliferation / regulation of cardiac muscle hypertrophy in response to stress / regulation of lung blood pressure / lymphatic endothelial cell differentiation / pulmonary valve development / regulation of endothelial cell proliferation / positive regulation of cell proliferation involved in heart morphogenesis / dorsal aorta morphogenesis / chondrocyte development / tricuspid valve morphogenesis / positive regulation of sarcomere organization / blood vessel maturation / atrial cardiac muscle tissue morphogenesis / negative regulation of cell proliferation involved in heart valve morphogenesis / negative regulation of endothelial cell differentiation / aortic valve development / positive regulation of epithelial cell differentiation / BMP binding / negative regulation of muscle cell differentiation / ventricular cardiac muscle cell development / venous blood vessel development / atrial septum morphogenesis / endocardial cushion development / maternal placenta development / proteoglycan biosynthetic process / endochondral bone morphogenesis / telethonin binding / positive regulation of cartilage development / positive regulation of chondrocyte differentiation / transforming growth factor beta receptor activity / lung vasculature development / lymphangiogenesis / mitral valve morphogenesis / BMP receptor complex / blood vessel endothelial cell proliferation involved in sprouting angiogenesis / retina vasculature development in camera-type eye / positive regulation of axon extension involved in axon guidance / positive regulation of endothelial cell differentiation / negative regulation of focal adhesion assembly / negative regulation of cardiac muscle hypertrophy / regulation of blood vessel endothelial cell migration / endothelial tube morphogenesis / artery development / transforming growth factor beta receptor activity, type I / activin receptor activity, type I / activin receptor activity, type II / BMP receptor activity / receptor protein serine/threonine kinase / transmembrane receptor protein serine/threonine kinase activity / transforming growth factor beta receptor activity, type II / transforming growth factor beta receptor activity, type III / activin binding / cellular response to BMP stimulus / Signaling by BMP / negative regulation of cell adhesion / activin receptor signaling pathway / receptor serine/threonine kinase binding / positive regulation of BMP signaling pathway / positive regulation of bicellular tight junction assembly / endothelial cell apoptotic process / heart trabecula formation / adult heart development / positive regulation of ossification / dorsal/ventral pattern formation / negative regulation of systemic arterial blood pressure / transforming growth factor beta binding / endothelial cell proliferation / limb development / wound healing, spreading of epidermal cells / negative regulation of endothelial cell migration / blood circulation / anterior/posterior pattern specification / cell surface receptor protein serine/threonine kinase signaling pathway / endocardial cushion morphogenesis / cardiac muscle cell proliferation / sarcomere organization / Molecules associated with elastic fibres / negative regulation of vasoconstriction / ventricular cardiac muscle tissue morphogenesis / ventricular septum morphogenesis / positive regulation of Notch signaling pathway / lung alveolus development / growth factor binding / blood vessel development / SMAD binding / positive regulation of cardiac muscle hypertrophy / negative regulation of endothelial cell proliferation / outflow tract morphogenesis / regulation of DNA replication / negative regulation of blood vessel endothelial cell migration / mesoderm formation / positive regulation of epithelial cell migration / positive regulation of SMAD protein signal transduction / blood vessel remodeling / regulation of cardiac muscle contraction / BMP signaling pathway / positive regulation of bone mineralization / positive regulation of osteoblast differentiation / positive regulation of cardiac muscle cell proliferation
Similarity search - Function
GS domain / Transforming growth factor beta type I GS-motif / GS domain profile. / GS motif / TGF-beta, propeptide / TGF-beta propeptide / Activin types I and II receptor domain / Activin types I and II receptor domain / Transforming growth factor beta, conserved site / TGF-beta family signature. ...GS domain / Transforming growth factor beta type I GS-motif / GS domain profile. / GS motif / TGF-beta, propeptide / TGF-beta propeptide / Activin types I and II receptor domain / Activin types I and II receptor domain / Transforming growth factor beta, conserved site / TGF-beta family signature. / Transforming growth factor-beta-related / Transforming growth factor-beta (TGF-beta) family / Transforming growth factor-beta, C-terminal / Transforming growth factor beta like domain / TGF-beta family profile. / Ser/Thr protein kinase, TGFB receptor / Cystine-knot cytokine / Snake toxin-like superfamily / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
Bone morphogenetic protein 10 / Activin receptor type-1-like / Bone morphogenetic protein receptor type-2
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.6 Å
AuthorsGuo, J. / Yu, M. / Read, R.J. / Li, W.
Funding support United Kingdom, 1items
OrganizationGrant numberCountry
British Heart FoundationPG/12/54/29734, PG/17/1/32532, FS/SBSRF/20/31005 United Kingdom
CitationJournal: Nat Commun / Year: 2022
Title: Crystal structures of BMPRII extracellular domain in binary and ternary receptor complexes with BMP10.
Authors: Guo, J. / Liu, B. / Thorikay, M. / Yu, M. / Li, X. / Tong, Z. / Salmon, R.M. / Read, R.J. / Ten Dijke, P. / Morrell, N.W. / Li, W.
History
DepositionSep 13, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0May 11, 2022Provider: repository / Type: Initial release
Revision 1.1Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.2Oct 23, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Bone morphogenetic protein 10
B: Bone morphogenetic protein 10
C: Bone morphogenetic protein 10
D: Bone morphogenetic protein 10
E: Serine/threonine-protein kinase receptor R3
F: Serine/threonine-protein kinase receptor R3
G: Serine/threonine-protein kinase receptor R3
H: Serine/threonine-protein kinase receptor R3
I: Bone morphogenetic protein receptor type-2
J: Bone morphogenetic protein receptor type-2
K: Bone morphogenetic protein receptor type-2
L: Bone morphogenetic protein receptor type-2


Theoretical massNumber of molelcules
Total (without water)147,26612
Polymers147,26612
Non-polymers00
Water905
1
A: Bone morphogenetic protein 10
B: Bone morphogenetic protein 10
E: Serine/threonine-protein kinase receptor R3
F: Serine/threonine-protein kinase receptor R3
I: Bone morphogenetic protein receptor type-2
J: Bone morphogenetic protein receptor type-2


Theoretical massNumber of molelcules
Total (without water)73,6336
Polymers73,6336
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: Bone morphogenetic protein 10
D: Bone morphogenetic protein 10
G: Serine/threonine-protein kinase receptor R3
H: Serine/threonine-protein kinase receptor R3
K: Bone morphogenetic protein receptor type-2
L: Bone morphogenetic protein receptor type-2


Theoretical massNumber of molelcules
Total (without water)73,6336
Polymers73,6336
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)63.761, 130.387, 88.825
Angle α, β, γ (deg.)90.000, 103.383, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 321 through 323 or (resid 324...
d_2ens_1(chain "B" and (resid 321 through 323 or (resid 324...
d_3ens_1(chain "C" and (resid 321 through 332 or (resid 333...
d_4ens_1(chain "D" and (resid 321 through 323 or (resid 324...
d_1ens_2(chain "E" and (resid 30 through 74 or (resid 75...
d_2ens_2(chain "F" and (resid 30 through 60 or (resid 61...
d_3ens_2(chain "G" and (resid 30 through 64 or (resid 65...
d_4ens_2(chain "H" and (resid 30 through 60 or (resid 61...
d_1ens_3(chain "I" and (resid 33 through 36 or (resid 37...
d_2ens_3(chain "J" and (resid 33 through 38 or resid 53...
d_3ens_3(chain "K" and (resid 33 through 38 or resid 53...
d_4ens_3(chain "L" and (resid 33 through 36 or (resid 37...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1ASNARGA1 - 104
d_21ens_1ASNARGB1 - 104
d_31ens_1ASNARGC1 - 104
d_41ens_1ASNARGD1 - 104
d_11ens_2PROASNE2 - 58
d_12ens_2TYRALAE60 - 77
d_21ens_2PROASNF1 - 57
d_22ens_2TYRALAF59 - 76
d_31ens_2PROASNG1 - 57
d_32ens_2TYRALAG59 - 76
d_41ens_2PROASNH1 - 57
d_42ens_2TYRALAH59 - 76
d_11ens_3LEUASPI4 - 9
d_12ens_3HISLYSI11 - 30
d_13ens_3ILEVALI32 - 80
d_21ens_3LEUASPJ1 - 6
d_22ens_3HISVALJ8 - 76
d_31ens_3LEUASPK3 - 8
d_32ens_3HISLYSK10 - 29
d_33ens_3ILEVALK31 - 79
d_41ens_3LEUASPL3 - 8
d_42ens_3HISLYSL10 - 29
d_43ens_3ILEVALL31 - 79

NCS ensembles :
ID
ens_1
ens_2
ens_3

NCS oper:
IDCodeMatrixVector
1given(-0.197051777367, 0.291199420604, -0.936148222493), (0.310069036136, -0.887345056615, -0.341285720961), (-0.930068701692, -0.357521534934, 0.0845609968737)38.255179729, 30.2788724478, 42.6797818577
2given(-0.809359007354, -0.0138820126491, -0.587150140033), (0.0214089048446, 0.998358859348, -0.0531154097752), (0.586923892859, -0.0555596768083, -0.807733536696)17.2974977075, -7.17936269421, 42.0272010593
3given(0.72485429914, -0.0066203070057, 0.688870391694), (0.32679549692, -0.876979787764, -0.352294131438), (0.606457705211, 0.480481657702, -0.633519082902)-37.7298965641, 20.7405403265, 29.9925427212
4given(-0.207326596948, 0.320251627604, -0.92436712253), (0.294129120158, -0.880772015647, -0.371118198326), (-0.933007900756, -0.348825961577, 0.0884121352346)37.9046353118, 31.6581584787, 42.3882387739
5given(-0.805972162219, 0.0293102074216, -0.591227355143), (0.00976921368398, 0.999295990968, 0.0362227124785), (0.591872820961, 0.0234186715295, -0.805691088216)18.4147502082, -11.3396830039, 42.4839650992
6given(0.704084979703, -0.044312205092, 0.708731803884), (0.36592707133, -0.832695484905, -0.415590673485), (0.60857351226, 0.551955304259, -0.570073348154)-39.1231573056, 24.0293353611, 27.6776532747
7given(-0.176349419599, 0.234648577094, -0.955950274583), (0.435759908717, -0.852209899907, -0.289571387493), (-0.882617801861, -0.467630550508, 0.0480362786902)40.6559560422, 29.8225116768, 47.062484609
8given(-0.793902935992, -0.0380314731994, -0.606853965357), (0.00558515467724, 0.997543787781, -0.0698226145749), (0.608018860128, -0.0588217519727, -0.791740530239)19.1280160951, -6.79227837723, 41.9741207846
9given(0.718297423604, 0.11610439151, 0.685980015391), (0.432218810347, -0.847099809196, -0.309206748377), (0.545193278779, 0.518595876895, -0.658652112455)-40.851104241, 20.2371811293, 29.5408232628

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Components

#1: Protein
Bone morphogenetic protein 10 / BMP-10


Mass: 12177.185 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: BMP10 / Cell line (production host): HEK EBNA / Production host: Homo sapiens (human) / References: UniProt: O95393
#2: Protein
Serine/threonine-protein kinase receptor R3 / SKR3 / Activin receptor-like kinase 1 / ALK-1 / TGF-B superfamily receptor type I / TSR-I


Mass: 10731.074 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ACVRL1, ACVRLK1, ALK1 / Production host: Escherichia coli (E. coli)
References: UniProt: P37023, receptor protein serine/threonine kinase
#3: Protein
Bone morphogenetic protein receptor type-2 / BMP type-2 receptor / BMPR-2 / Bone morphogenetic protein receptor type II / BMP type II receptor / BMPR-II


Mass: 13908.316 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: BMPR2, PPH1 / Production host: Escherichia coli (E. coli)
References: UniProt: Q13873, receptor protein serine/threonine kinase
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.43 Å3/Da / Density % sol: 49.36 % / Description: Cluster of thin plates/blades
Crystal growTemperature: 294.15 K / Method: vapor diffusion, hanging drop / pH: 7.5 / Details: 20% PEG 3350 0.2 M Calcium acetate hydrate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.91188 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Nov 26, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.91188 Å / Relative weight: 1
ReflectionResolution: 3.6→130.39 Å / Num. obs: 11220 / % possible obs: 99.9 % / Redundancy: 4.9 % / Biso Wilson estimate: 53 Å2 / CC1/2: 0.977 / Rmerge(I) obs: 0.331 / Rpim(I) all: 0.163 / Rrim(I) all: 0.371 / Net I/σ(I): 2.5
Reflection shellResolution: 3.6→3.94 Å / Redundancy: 4.9 % / Rmerge(I) obs: 1.223 / Mean I/σ(I) obs: 0.9 / Num. unique obs: 789 / CC1/2: 0.673 / Rpim(I) all: 0.604 / Rrim(I) all: 1.369 / % possible all: 99.5

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Processing

Software
NameVersionClassification
DIALS1.14.13data reduction
Aimless0.7.4data scaling
PHASER2.8.3phasing
PHENIX1.19.2_4158refinement
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6SF3, 7PPA

6sf3

7ppa


Resolution: 3.6→86.41 Å / SU ML: 0.4875 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 29.5195
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2825 541 4.83 %
Rwork0.2436 10658 -
obs0.2455 11199 68.2 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 73.04 Å2
Refinement stepCycle: LAST / Resolution: 3.6→86.41 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8126 0 0 5 8131
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00298342
X-RAY DIFFRACTIONf_angle_d0.621911332
X-RAY DIFFRACTIONf_chiral_restr0.04561243
X-RAY DIFFRACTIONf_plane_restr0.0051453
X-RAY DIFFRACTIONf_dihedral_angle_d3.86141126
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS0.822770441852
ens_1d_3AX-RAY DIFFRACTIONTorsion NCS0.565951381743
ens_1d_4AX-RAY DIFFRACTIONTorsion NCS1.04610925931
ens_2d_2EX-RAY DIFFRACTIONTorsion NCS1.52149833826
ens_2d_3EX-RAY DIFFRACTIONTorsion NCS1.62288379081
ens_2d_4EX-RAY DIFFRACTIONTorsion NCS1.63291716889
ens_3d_2IX-RAY DIFFRACTIONTorsion NCS1.25982449558
ens_3d_3IX-RAY DIFFRACTIONTorsion NCS1.24943427366
ens_3d_4IX-RAY DIFFRACTIONTorsion NCS1.4838210775
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.6-3.960.311090.26771918X-RAY DIFFRACTION49.49
3.96-4.540.31511080.23322276X-RAY DIFFRACTION58.13
4.54-5.710.26891380.23112707X-RAY DIFFRACTION69.36
5.72-86.410.26281860.24913757X-RAY DIFFRACTION94.69
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.660038787282-0.1486313904580.2684348107850.7940100812030.06566699498120.9416940367640.2287990493910.0250396666148-0.1013403310170.0786465568939-0.0139265920505-0.06789809167560.204540577705-0.128535476139-0.001686084844470.487892963697-0.120345300049-0.04673274848160.352193823061-0.0930468674561-0.00640475772842-9.5489872949510.012581051142.2181877104
20.952783051634-0.200253323919-0.05137288394790.491278173256-0.1409469804670.5220655781820.101456185426-0.498370154805-0.1730780509190.0818562701314-0.0235914158309-0.2077106887180.151745591010.362077754206-0.1481279630130.555284394290.0331299043336-0.4135214111290.434130643442-0.08252129466260.2783443421223.70640145713.8646059038251.5983634304
31.247456492761.83316990686-0.8539589980054.04507624462-0.7806377094040.720716676478-0.1699103202390.00662263566358-0.0410872680364-0.05531147027040.1574198634530.0821004592761-0.3030201772790.09357613568310.0189746586060.830983883968-0.00311757372543-0.1548058533110.3238373587420.147969026930.277485928560.2905905439570.6469161654321.72768346973
41.521515476441.76576749594-0.7723061100793.43918938416-1.408833235910.5796635703570.07463128486020.2054706571780.1313322009710.1305323888610.1832733634050.514696632604-0.0964916794066-0.347907359614-0.3045387610330.9475045404310.09681350331070.05070670262910.3590851322520.1363068342060.274399481208-15.7559762784-6.151976849182.30460937379
51.50322044153-0.3260395832250.7144499292270.34148487544-0.8497061133884.7823946103-0.195989731516-0.1563863854350.07857543978850.4351895855360.0353814731160.129515025487-0.4898003208620.447845139225-0.1700068240430.803286884563-0.121890750860.2557431033070.232978208627-0.02220895947630.263216581372-2.33653392315-17.989999522243.1402769225
63.67431884738-0.4689501981180.8769331908622.009270826930.8170067046982.2609806950.0283364966993-0.373246037288-0.2477659100730.1967113165740.170464871929-0.331401939679-0.1382261494760.284849503157-0.1924183279540.490498844365-0.1830781552930.1441799881250.246828189539-0.08691862467090.213181539808-7.6846779284230.586631510154.763302065
72.824352210120.5210083739090.01587389423730.61961057892-0.1446241242931.415194689520.08985160982910.555844948260.0741807461335-0.3509696415170.03544229373510.106284194036-0.322777031697-0.0110279583120.0534637067081.275715852260.2183124863670.01458007259980.4248318955280.0190685854460.220090460962-5.64344685278-27.92377282215.52473036015
81.889013152290.731469878726-1.213585410620.81775961112-1.508844466583.64725578298-0.1289198367740.2885355198440.041048417728-0.2432463881880.109759579610.0004330962459890.0206977825906-0.252899145526-0.1841712650310.7201285033190.04643610667110.1663433357230.2686642563470.1475601730610.231590806826-9.2331598212920.0782139083-8.56369955051
98.70310856526-1.47268604149-2.256480427933.702238942380.2065225442259.579902436050.4500720312590.281303347541-0.3575261715060.0501824847902-0.4254448877830.3912452147690.200315423645-1.20206974139-0.001790337397290.870308576422-0.098548690078-0.02746378629490.64007807535-0.08360143359370.220471581256-19.695226922423.772245558821.7906782201
101.56859129794-3.774206774753.264279435799.09569176763-7.726977736557.606044276110.0201128684064-0.1506276730030.3681351896050.5621706354390.160115450707-1.09160688792-0.1872931925430.710972488054-0.1828633440520.821470059191-0.1119297557560.05140408088671.37294933226-0.2507982019820.92804967731428.631630576-5.7421074190453.9590664729
112.79935783970.279679109783-0.5347222200163.214603253871.913826572231.514688025040.102248859889-0.591782878322-0.553086434556-0.3030232522940.325689070353-0.153517078049-0.375019655567-0.122667924566-0.434811705061.7414563667-0.17058726336-0.7574120114551.355078104040.09942537920831.0137708363220.881328426615.656307220510.9302813611
126.86423483336-3.03716765474-1.64617151538.37072650527.913974771867.781320503070.1240646595670.382498984127-0.267240905902-0.632356490514-0.4947566436631.64921227499-0.708981096205-1.385279788040.343717798450.7654624402450.1378226343310.04776348330330.9940495383240.360952860641.10133107153-36.7531049026-15.22196202217.0566494711
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11(chain 'A' and resid 321 through 424)AA321 - 4241 - 104
22(chain 'B' and resid 321 through 424)BB321 - 4241 - 104
33(chain 'C' and resid 321 through 424)CC321 - 4241 - 104
44(chain 'D' and resid 321 through 424)DD321 - 4241 - 104
55(chain 'E' and resid 29 through 105)EE29 - 1051 - 77
66(chain 'F' and resid 30 through 105)FF30 - 1051 - 76
77(chain 'G' and resid 30 through 105)GG30 - 1051 - 76
88(chain 'H' and resid 30 through 105)HH30 - 1051 - 76
99(chain 'I' and resid 30 through 130)II30 - 1301 - 85
1010(chain 'J' and resid 33 through 127)JJ33 - 1271 - 78
1111(chain 'K' and resid 31 through 132)KK31 - 1321 - 86
1212(chain 'L' and resid 31 through 125)LL31 - 1251 - 79

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