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- PDB-7pen: Crystal Structure of Two-Domain Laccase mutant Y230A from Strepto... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7pen | ||||||
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Title | Crystal Structure of Two-Domain Laccase mutant Y230A from Streptomyces griseoflavus | ||||||
![]() | Two-domain laccase | ||||||
![]() | OXIDOREDUCTASE / Two-Domain Laccase / laccase / Streptomyces griseoflavus | ||||||
Function / homology | ![]() hydroquinone:oxygen oxidoreductase activity / laccase / copper ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gabdulkhakov, A. / Tishchenko, S. / Kolyadenko, I. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Engineering the Catalytic Properties of Two-Domain Laccase from Streptomyces griseoflavus Ac-993. Authors: Kolyadenko, I. / Scherbakova, A. / Kovalev, K. / Gabdulkhakov, A. / Tishchenko, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 792.6 KB | Display | ![]() |
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PDB format | ![]() | 582.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 467.6 KB | Display | ![]() |
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Full document | ![]() | 477.4 KB | Display | |
Data in XML | ![]() | 71.3 KB | Display | |
Data in CIF | ![]() | 103.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7pesC ![]() 7pfrC ![]() 7ptmC ![]() 7pu0C ![]() 7puhC ![]() 5lhlS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34659.543 Da / Num. of mol.: 6 / Mutation: Y230A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-CU / #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 40.02 % |
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 15 % v/v PEG Smear High, 0.15 M Ammonium acetate, 0.1 M Sodium citrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 17, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97242 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→50 Å / Num. obs: 220573 / % possible obs: 99.9 % / Redundancy: 5.63 % / Biso Wilson estimate: 20.11 Å2 / CC1/2: 0.99 / Rrim(I) all: 0.1 / Net I/σ(I): 10.93 |
Reflection shell | Resolution: 1.6→1.64 Å / Num. unique obs: 16337 / CC1/2: 0.68 / Rrim(I) all: 1.13 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5LHL Resolution: 1.6→47.1 Å / SU ML: 0.1986 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 16.3746 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 24.5 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→47.1 Å
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Refine LS restraints |
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LS refinement shell |
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