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- PDB-6fc7: Crystal Structure of Two-Domain Laccase mutant H165F from Strepto... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6fc7 | ||||||
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Title | Crystal Structure of Two-Domain Laccase mutant H165F from Streptomyces griseoflavus with high copper ions occupancy | ||||||
![]() | Two-domain laccase | ||||||
![]() | OXIDOREDUCTASE / Two-Domain Laccase / laccase / Streptomyces griseoflavus | ||||||
Function / homology | ![]() hydroquinone:oxygen oxidoreductase activity / laccase / copper ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gabdulkhakov, A.G. / Tishchenko, T.V. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Investigations of Accessibility of T2/T3 Copper Center of Two-Domain Laccase fromStreptomyces griseoflavusAc-993. Authors: Gabdulkhakov, A. / Kolyadenko, I. / Kostareva, O. / Mikhaylina, A. / Oliveira, P. / Tamagnini, P. / Lisov, A. / Tishchenko, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 649.5 KB | Display | ![]() |
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PDB format | ![]() | 532.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5mkmSC ![]() 6fdjC ![]() 6rh9C ![]() 6rhqC ![]() 6s0oC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
-Protein , 1 types, 12 molecules ABCDEFGHIJKL
#1: Protein | Mass: 34760.664 Da / Num. of mol.: 12 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 5 types, 639 molecules ![](data/chem/img/CU.gif)
![](data/chem/img/PER.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/OXY.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/PER.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/OXY.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-CU / #3: Chemical | ChemComp-PER / #4: Chemical | ChemComp-GOL / | #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.22 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / Details: 20% PEG 6K, 0.1M Bicine, pH 9 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 27, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.27516 Å / Relative weight: 1 |
Reflection | Resolution: 1.92→50 Å / Num. obs: 235591 / % possible obs: 94.3 % / Redundancy: 3.59 % / Net I/σ(I): 7.88 |
Reflection shell | Resolution: 1.92→2.05 Å / Mean I/σ(I) obs: 1.57 / Num. unique obs: 41396 / % possible all: 92.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5MKM Resolution: 1.95→49.415 Å / Cross valid method: FREE R-VALUE / σ(F): 7.6 / Phase error: 33.13
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→49.415 Å
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Refine LS restraints |
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LS refinement shell |
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