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- PDB-5o4i: Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5o4i | ||||||
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Title | Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus dialyzed against solution containing 0.25 mM copper sulfate | ||||||
![]() | Two-domain laccase | ||||||
![]() | OXIDOREDUCTASE / Two-Domain Laccase / laccase / Streptomyces griseoflavus | ||||||
Function / homology | ![]() hydroquinone:oxygen oxidoreductase activity / laccase / copper ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gabdulkhakov, A.G. / Tishchenko, T.V. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Incorporation of Copper Ions into T2/T3 Centers of Two-Domain Laccases. Authors: Gabdulkhakov, A.G. / Kostareva, O.S. / Kolyadenko, I.A. / Mikhaylina, A.O. / Trubitsina, L.I. / Tishchenko, S.V. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 664 KB | Display | ![]() |
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PDB format | ![]() | 544.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 559.3 KB | Display | ![]() |
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Full document | ![]() | 590 KB | Display | |
Data in XML | ![]() | 124.1 KB | Display | |
Data in CIF | ![]() | 171.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5o3kSC ![]() 5o4qC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34697.523 Da / Num. of mol.: 12 Source method: isolated from a genetically manipulated source Details: mutations M54L, M64L, M96L / Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-CU / #3: Chemical | ChemComp-GOL / #4: Chemical | ChemComp-SO4 / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.84 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8 / Details: 23% PEG 4000, 0.05M Tris-HCl |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Oct 7, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9687 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→50 Å / Num. obs: 283032 / % possible obs: 92.6 % / Redundancy: 2.07 % / Net I/σ(I): 6.81 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5O3K Resolution: 1.8→50 Å / Cor.coef. Fo:Fc: 0.965 / Cor.coef. Fo:Fc free: 0.944 / SU B: 3.81 / SU ML: 0.111 / Cross valid method: THROUGHOUT / ESU R: 0.136 / ESU R Free: 0.132 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.15 Å2
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Refinement step | Cycle: 1 / Resolution: 1.8→50 Å
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Refine LS restraints |
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