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Yorodumi- PDB-7osa: Pre-translocation complex of 80 S.cerevisiae ribosome with eEF2 a... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7osa | ||||||
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Title | Pre-translocation complex of 80 S.cerevisiae ribosome with eEF2 and ligands | ||||||
Components |
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Keywords | RIBOSOME / translocation / eEF2 / tRNA / mRNA / eukaryotic ribosome | ||||||
Function / homology | Function and homology information ribosomal subunit / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression ...ribosomal subunit / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / protein-RNA complex assembly / translation elongation factor activity / maturation of LSU-rRNA / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / macroautophagy / modification-dependent protein catabolic process / protein tag activity / rRNA processing / large ribosomal subunit / ribosome biogenesis / ribosome binding / ribosomal small subunit assembly / large ribosomal subunit rRNA binding / 5S rRNA binding / small ribosomal subunit / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / translation / ribonucleoprotein complex / GTPase activity / mRNA binding / ubiquitin protein ligase binding / nucleolus / GTP binding / RNA binding / zinc ion binding / nucleus / metal ion binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å | ||||||
Authors | Djumagulov, M. / Jenner, L. / Rozov, A. / Demeshkina, N. / Yusupov, M. / Yusupova, G. | ||||||
Citation | Journal: Nature / Year: 2021 Title: Accuracy mechanism of eukaryotic ribosome translocation. Authors: Djumagulov, M. / Demeshkina, N. / Jenner, L. / Rozov, A. / Yusupov, M. / Yusupova, G. #1: Journal: Acta Crystallogr., Sect. D: Biol. Crystallogr. / Year: 2012 Title: Towards automated crystallographic structure refinement with phenix.refine. Authors: Adams, P.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7osa.cif.gz | 5.2 MB | Display | PDBx/mmCIF format |
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PDB format | pdb7osa.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 7osa.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7osa_validation.pdf.gz | 1.2 MB | Display | wwPDB validaton report |
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Full document | 7osa_full_validation.pdf.gz | 1.7 MB | Display | |
Data in XML | 7osa_validation.xml.gz | 519.6 KB | Display | |
Data in CIF | 7osa_validation.cif.gz | 763.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/os/7osa ftp://data.pdbj.org/pub/pdb/validation_reports/os/7osa | HTTPS FTP |
-Related structure data
Related structure data | 7osmC 4v88S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-RNA chain , 6 types, 6 molecules 25SAB58S18SPSITmRNA
#1: RNA chain | Mass: 1097492.875 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) |
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#2: RNA chain | Mass: 38951.105 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 1329886537 |
#3: RNA chain | Mass: 241543.234 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) |
#46: RNA chain | Mass: 579126.562 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 1329886537 |
#81: RNA chain | Mass: 24801.801 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#82: RNA chain | Mass: 935.620 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Saccharomyces cerevisiae (brewer's yeast) |
+Protein , 25 types, 25 molecules uL10uL4uL5uL22eL18eL21eL30eL31eL32eL33uL29eL37eL38eL40eL42uS3uS7uS4uS9eS17eS28uS14RACKeS31eEF2
+60S ribosomal protein ... , 27 types, 27 molecules uL2uL3uL18eL6uL30eL8uL6uL16eL13eL14eL15uL13eL19eL20eL22uL14eL24uL23uL24eL27uL15eL29eL34eL36eL39eL43uL11
-Protein/peptide , 1 types, 1 molecules eL41
#43: Protein/peptide | Mass: 3354.243 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) |
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+40S ribosomal protein ... , 24 types, 24 molecules uS2eS1uS5eS4eS6eS7eS8eS10uS17eS12uS15uS11uS19uS13eS19uS10eS21uS8uS12eS24eS25eS26eS27eS30
-Non-polymers , 3 types, 499 molecules
#84: Chemical | ChemComp-MG / #85: Chemical | ChemComp-ZN / #86: Chemical | ChemComp-GTP / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.03 Å3/Da / Density % sol: 59.43 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: PEG10K, KSCN, TRIS-HAC |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X6A / Wavelength: 1 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Aug 8, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3→154.68 Å / Num. obs: 734615 / % possible obs: 99.98 % / Redundancy: 206 % / Biso Wilson estimate: 112.7 Å2 / CC1/2: 0.99 / Net I/σ(I): 20.12 |
Reflection shell | Resolution: 3→3.1 Å / Mean I/σ(I) obs: 0.95 / Num. unique obs: 72990 / CC1/2: 0.23 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4V88 Resolution: 3→154.68 Å / SU ML: 0.5314 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.8096 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 119.97 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→154.68 Å
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Refine LS restraints |
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LS refinement shell |
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