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- PDB-7osa: Pre-translocation complex of 80 S.cerevisiae ribosome with eEF2 a... -

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Basic information

Entry
Database: PDB / ID: 7osa
TitlePre-translocation complex of 80 S.cerevisiae ribosome with eEF2 and ligands
Components
  • (40S ribosomal protein ...) x 24
  • (60S ribosomal protein ...) x 27
  • 18S rRNA
  • 25S rRNA
  • 5.8S rRNA
  • 5S
  • 60S acidic ribosomal protein P0
  • BJ4_G0001880.mRNA.1.CDS.1
  • BJ4_G0003770.mRNA.1.CDS.1
  • BJ4_G0005750.mRNA.1.CDS.1
  • BJ4_G0008010.mRNA.1.CDS.1
  • BJ4_G0008090.mRNA.1.CDS.1
  • BJ4_G0008850.mRNA.1.CDS.1
  • BJ4_G0020000.mRNA.1.CDS.1
  • BJ4_G0020710.mRNA.1.CDS.1
  • BJ4_G0022010.mRNA.1.CDS.1
  • BJ4_G0025510.mRNA.1.CDS.1
  • BJ4_G0026100.mRNA.1.CDS.1
  • BJ4_G0027750.mRNA.1.CDS.1
  • BJ4_G0032190.mRNA.1.CDS.1
  • BJ4_G0033900.mRNA.1.CDS.1
  • BJ4_G0044250.mRNA.1.CDS.1
  • BJ4_G0045400.mRNA.1.CDS.1
  • Elongation factor 2
  • HLJ1_G0030400.mRNA.1.CDS.1
  • HN1_G0013350.mRNA.1.CDS.1
  • MRNA
  • Psite tRNA-Phe
  • Ribosomal protein L37
  • Rps5p
  • Ubiquitin
  • Ubiquitin-40S ribosomal protein S31
  • eL41
  • eS28
KeywordsRIBOSOME / translocation / eEF2 / tRNA / mRNA / eukaryotic ribosome
Function / homology
Function and homology information


ribosomal subunit / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression ...ribosomal subunit / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / protein-RNA complex assembly / translation elongation factor activity / maturation of LSU-rRNA / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / macroautophagy / modification-dependent protein catabolic process / protein tag activity / rRNA processing / large ribosomal subunit / ribosome biogenesis / ribosome binding / ribosomal small subunit assembly / large ribosomal subunit rRNA binding / 5S rRNA binding / small ribosomal subunit / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / translation / ribonucleoprotein complex / GTPase activity / mRNA binding / ubiquitin protein ligase binding / nucleolus / GTP binding / RNA binding / zinc ion binding / nucleus / metal ion binding / cytosol / cytoplasm
Similarity search - Function
60S acidic ribosomal protein P0 / Elongation Factor G, domain II / Elongation Factor G, domain III / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / Elongation factor G C-terminus / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / 50S ribosomal protein L10, insertion domain superfamily ...60S acidic ribosomal protein P0 / Elongation Factor G, domain II / Elongation Factor G, domain III / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / Elongation factor G C-terminus / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / 50S ribosomal protein L10, insertion domain superfamily / : / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / EF-G domain III/V-like / Tr-type G domain, conserved site / Translational (tr)-type guanine nucleotide-binding (G) domain signature. / : / Ribosomal protein S26e signature. / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / S27a-like superfamily / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / 40S Ribosomal protein S10 / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L44e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L19, eukaryotic / Ribosomal protein L10e / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S2, eukaryotic/archaeal / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein S30 / Ribosomal protein S30 / Ribosomal protein S7e signature. / Ribosomal protein L44e / Ribosomal protein L24e, conserved site / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein L44 / Ribosomal protein L24e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L6e signature. / Translation elongation factor EFTu-like, domain 2 / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal L40e family / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein S27e signature. / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Eukaryotic Ribosomal Protein L27, KOW domain
Similarity search - Domain/homology
GUANOSINE-5'-TRIPHOSPHATE / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Rps5p / Small ribosomal subunit protein eS1 / RPS22A isoform 1 / 60S acidic ribosomal protein P0 ...GUANOSINE-5'-TRIPHOSPHATE / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Rps5p / Small ribosomal subunit protein eS1 / RPS22A isoform 1 / 60S acidic ribosomal protein P0 / RPS31 isoform 1 / 40S ribosomal protein S26 / RPL38 isoform 1 / RPL10 isoform 1 / RPS29A isoform 1 / RPS20 isoform 1 / RPS2 isoform 1 / 60S ribosomal protein L29 / RPL24A isoform 1 / 40S ribosomal protein S25 / RPL5 isoform 1 / 40S ribosomal protein S8 / RPL12A isoform 1 / RPL4A isoform 1 / Large ribosomal subunit protein uL3 / RPS15 isoform 1 / RPS28A isoform 1 / RPL11A isoform 1 / Elongation factor 2 / 40S ribosomal protein S12 / 60S ribosomal protein L2-B / 40S ribosomal protein S13 / 60S ribosomal protein L31-A / 40S ribosomal protein S11-B / EM14S01-3B_G0023620.mRNA.1.CDS.1 / 60S ribosomal protein L25 / BJ4_G0018510.mRNA.1.CDS.1 / 40S ribosomal protein S4 / 60S ribosomal protein L23-B / 60S ribosomal protein L16-A / 60S ribosomal protein L28 / 60S ribosomal protein L34-A / : / 40S ribosomal protein S10-A / 60S ribosomal protein L27 / 40S ribosomal protein S0 / : / 60S ribosomal protein L22-A / 40S ribosomal protein S30 / 60S ribosomal protein L18-B / 40S ribosomal protein S14-A / 40S ribosomal protein S7 / 60S ribosomal protein L13 / 40S ribosomal protein S18-B / 40S ribosomal protein S6 / 60S ribosomal protein L39 / 40S ribosomal protein S24 / 40S ribosomal protein S23 / 60S ribosomal protein L42-B / 60S ribosomal protein L17-A / 40S ribosomal protein S21 / Ribosomal protein L37 / EM14S01-3B_G0023350.mRNA.1.CDS.1 / EM14S01-3B_G0022060.mRNA.1.CDS.1 / 60S ribosomal protein L6 / 40S ribosomal protein S19-A / EM14S01-3B_G0017920.mRNA.1.CDS.1 / 60S ribosomal protein L20 / Ubiquitin / EM14S01-3B_G0041280.mRNA.1.CDS.1 / HN1_G0013350.mRNA.1.CDS.1 / EM14S01-3B_G0018500.mRNA.1.CDS.1 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein eL8A / Small ribosomal subunit protein eS27A / Large ribosomal subunit protein eL14A
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
Escherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å
AuthorsDjumagulov, M. / Jenner, L. / Rozov, A. / Demeshkina, N. / Yusupov, M. / Yusupova, G.
Citation
Journal: Nature / Year: 2021
Title: Accuracy mechanism of eukaryotic ribosome translocation.
Authors: Djumagulov, M. / Demeshkina, N. / Jenner, L. / Rozov, A. / Yusupov, M. / Yusupova, G.
#1: Journal: Acta Crystallogr., Sect. D: Biol. Crystallogr. / Year: 2012
Title: Towards automated crystallographic structure refinement with phenix.refine.
Authors: Adams, P.D.
History
DepositionJun 8, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0Dec 8, 2021Provider: repository / Type: Initial release
Revision 1.1Dec 22, 2021Group: Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed ..._citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID / _citation_author.name
Revision 1.2Dec 29, 2021Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.3Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
25S: 25S rRNA
AB: 5S
58S: 5.8S rRNA
uL10: 60S acidic ribosomal protein P0
uL2: 60S ribosomal protein L2-B
uL3: 60S ribosomal protein L3
uL4: BJ4_G0008850.mRNA.1.CDS.1
uL18: 60S ribosomal protein L5
eL6: 60S ribosomal protein L6
uL30: 60S ribosomal protein L7-A
eL8: 60S ribosomal protein L8-A
uL6: 60S ribosomal protein L9-A
uL16: 60S ribosomal protein L10
uL5: BJ4_G0027750.mRNA.1.CDS.1
eL13: 60S ribosomal protein L13
eL14: 60S ribosomal protein L14-A
eL15: 60S ribosomal protein L15-A
uL13: 60S ribosomal protein L16-A
uL22: BJ4_G0005750.mRNA.1.CDS.1
eL18: BJ4_G0033900.mRNA.1.CDS.1
eL19: 60S ribosomal protein L19-A
eL20: 60S ribosomal protein L20
eL21: BJ4_G0003770.mRNA.1.CDS.1
eL22: 60S ribosomal protein L22-A
uL14: 60S ribosomal protein L23-B
eL24: 60S ribosomal protein L24-A
uL23: 60S ribosomal protein L25
uL24: 60S ribosomal protein L26-A
eL27: 60S ribosomal protein L27
uL15: 60S ribosomal protein L28
eL29: 60S ribosomal protein L29
eL30: BJ4_G0020000.mRNA.1.CDS.1
eL31: BJ4_G0008090.mRNA.1.CDS.1
eL32: HN1_G0013350.mRNA.1.CDS.1
eL33: BJ4_G0025510.mRNA.1.CDS.1
eL34: 60S ribosomal protein L34-A
uL29: BJ4_G0044250.mRNA.1.CDS.1
eL36: 60S ribosomal protein L36-A
eL37: Ribosomal protein L37
eL38: BJ4_G0032190.mRNA.1.CDS.1
eL39: 60S ribosomal protein L39
eL40: Ubiquitin
eL41: eL41
eL42: BJ4_G0001880.mRNA.1.CDS.1
eL43: 60S ribosomal protein L43-A
18S: 18S rRNA
uS2: 40S ribosomal protein S0
eS1: 40S ribosomal protein S1
uS5: 40S ribosomal protein S2
uS3: BJ4_G0045400.mRNA.1.CDS.1
eS4: 40S ribosomal protein S4
uS7: Rps5p
eS6: 40S ribosomal protein S6
eS7: 40S ribosomal protein S7
eS8: 40S ribosomal protein S8
uS4: BJ4_G0026100.mRNA.1.CDS.1
eS10: 40S ribosomal protein S10-A
uS17: 40S ribosomal protein S11-B
eS12: 40S ribosomal protein S12
uS15: 40S ribosomal protein S13
uS11: 40S ribosomal protein S14-A
uS19: 40S ribosomal protein S15
uS9: BJ4_G0008010.mRNA.1.CDS.1
eS17: BJ4_G0020710.mRNA.1.CDS.1
uS13: 40S ribosomal protein S18-B
eS19: 40S ribosomal protein S19-A
uS10: 40S ribosomal protein S20
eS21: 40S ribosomal protein S21
uS8: 40S ribosomal protein S22-A
uS12: 40S ribosomal protein S23
eS24: 40S ribosomal protein S24
eS25: 40S ribosomal protein S25
eS26: 40S ribosomal protein S26
eS27: 40S ribosomal protein S27-A
eS28: eS28
uS14: HLJ1_G0030400.mRNA.1.CDS.1
eS30: 40S ribosomal protein S30
RACK: BJ4_G0022010.mRNA.1.CDS.1
eS31: Ubiquitin-40S ribosomal protein S31
eEF2: Elongation factor 2
PSIT: Psite tRNA-Phe
mRNA: MRNA
uL11: 60S ribosomal protein L12-B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)3,479,639582
Polymers3,466,68383
Non-polymers12,956499
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)227.110, 309.350, 527.960
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2

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Components

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RNA chain , 6 types, 6 molecules 25SAB58S18SPSITmRNA

#1: RNA chain 25S rRNA


Mass: 1097492.875 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast)
#2: RNA chain 5S


Mass: 38951.105 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 1329886537
#3: RNA chain 5.8S rRNA


Mass: 241543.234 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast)
#46: RNA chain 18S rRNA


Mass: 579126.562 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: GenBank: 1329886537
#81: RNA chain Psite tRNA-Phe


Mass: 24801.801 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli)
#82: RNA chain MRNA


Mass: 935.620 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Saccharomyces cerevisiae (brewer's yeast)

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Protein , 25 types, 25 molecules uL10uL4uL5uL22eL18eL21eL30eL31eL32eL33uL29eL37eL38eL40eL42uS3uS7uS4uS9eS17eS28uS14RACKeS31eEF2

#4: Protein 60S acidic ribosomal protein P0


Mass: 33749.121 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5PT43
#7: Protein BJ4_G0008850.mRNA.1.CDS.1 / BJ4_G0046040.mRNA.1.CDS.1 / EM14S01-3B_G0043150.mRNA.1.CDS.1 / HLJ1_G0008630.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0046040.mRNA.1.CDS.1 / EM14S01-3B_G0043150.mRNA.1.CDS.1 / HLJ1_G0008630.mRNA.1.CDS.1 / HLJ1_G0043850.mRNA.1.CDS.1 / HN1_G0043660.mRNA.1.CDS.1 / JXXY16_1_G0043210.mRNA.1.CDS.1 / SX2_G0043730.mRNA.1.CDS.1 / XXYS1_4_G0045660.mRNA.1.CDS.1 / Y55_G0043700.mRNA.1.CDS.1


Mass: 39159.125 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5Q3W0
#14: Protein BJ4_G0027750.mRNA.1.CDS.1 / BJ4_G0056660.mRNA.1.CDS.1 / EM14S01-3B_G0020140.mRNA.1.CDS.1 / HLJ1_G0025100.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0056660.mRNA.1.CDS.1 / EM14S01-3B_G0020140.mRNA.1.CDS.1 / HLJ1_G0025100.mRNA.1.CDS.1 / HLJ1_G0056670.mRNA.1.CDS.1 / HN1_G0025110.mRNA.1.CDS.1 / JXXY16_1_G0020160.mRNA.1.CDS.1 / SX2_G0020670.mRNA.1.CDS.1 / XXYS1_4_G0022650.mRNA.1.CDS.1 / Y55_G0020830.mRNA.1.CDS.1


Mass: 19755.691 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5Q7I9
#19: Protein BJ4_G0005750.mRNA.1.CDS.1 / BJ4_G0031710.mRNA.1.CDS.1 / EM14S01-3B_G0003030.mRNA.1.CDS.1 / HLJ1_G0003100.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0031710.mRNA.1.CDS.1 / EM14S01-3B_G0003030.mRNA.1.CDS.1 / HLJ1_G0003100.mRNA.1.CDS.1 / HLJ1_G0031620.mRNA.1.CDS.1 / HN1_G0003070.mRNA.1.CDS.1 / JXXY16_1_G0003160.mRNA.1.CDS.1 / SX2_G0003110.mRNA.1.CDS.1 / XXYS1_4_G0005910.mRNA.1.CDS.1 / Y55_G0003310.mRNA.1.CDS.1


Mass: 20589.518 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1BCL1
#20: Protein BJ4_G0033900.mRNA.1.CDS.1 / BJ4_G0048190.mRNA.1.CDS.1 / EM14S01-3B_G0025830.mRNA.1.CDS.1 / HN1_G0036520.mRNA.1.CDS.1 / JXXY16_1_ ...BJ4_G0048190.mRNA.1.CDS.1 / EM14S01-3B_G0025830.mRNA.1.CDS.1 / HN1_G0036520.mRNA.1.CDS.1 / JXXY16_1_G0025960.mRNA.1.CDS.1 / SX2_G0009590.mRNA.1.CDS.1 / SX2_G0031330.mRNA.1.CDS.1 / XXYS1_4_G0011980.mRNA.1.CDS.1 / Y55_G0009820.mRNA.1.CDS.1 / Y55_G0036540.mRNA.1.CDS.1


Mass: 20609.252 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1A7F6
#23: Protein BJ4_G0003770.mRNA.1.CDS.1 / BJ4_G0018510.mRNA.1.CDS.1


Mass: 18279.266 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0YTI6
#32: Protein BJ4_G0020000.mRNA.1.CDS.1


Mass: 11430.364 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0ZM08
#33: Protein BJ4_G0008090.mRNA.1.CDS.1 / BJ4_G0045320.mRNA.1.CDS.1 / EM14S01-3B_G0042370.mRNA.1.CDS.1 / HLJ1_G0007930.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0045320.mRNA.1.CDS.1 / EM14S01-3B_G0042370.mRNA.1.CDS.1 / HLJ1_G0007930.mRNA.1.CDS.1 / HLJ1_G0043110.mRNA.1.CDS.1 / HN1_G0042950.mRNA.1.CDS.1 / JXXY16_1_G0042500.mRNA.1.CDS.1 / SX2_G0043010.mRNA.1.CDS.1 / XXYS1_4_G0044940.mRNA.1.CDS.1 / Y55_G0042960.mRNA.1.CDS.1


Mass: 12980.158 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0YFR2
#34: Protein HN1_G0013350.mRNA.1.CDS.1


Mass: 14809.441 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A7I9CD28
#35: Protein BJ4_G0025510.mRNA.1.CDS.1 / BJ4_G0054440.mRNA.1.CDS.1 / EM14S01-3B_G0017920.mRNA.1.CDS.1 / HLJ1_G0022850.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0054440.mRNA.1.CDS.1 / EM14S01-3B_G0017920.mRNA.1.CDS.1 / HLJ1_G0022850.mRNA.1.CDS.1 / HLJ1_G0054430.mRNA.1.CDS.1 / HN1_G0022890.mRNA.1.CDS.1 / JXXY16_1_G0017940.mRNA.1.CDS.1 / SX2_G0018420.mRNA.1.CDS.1 / XXYS1_4_G0020460.mRNA.1.CDS.1 / Y55_G0018560.mRNA.1.CDS.1


Mass: 12177.130 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1C3H0
#37: Protein BJ4_G0044250.mRNA.1.CDS.1 / EM14S01-3B_G0041280.mRNA.1.CDS.1 / HN1_G0041880.mRNA.1.CDS.1 / JXXY16_1_G0041430.mRNA.1.CDS.1 / ...EM14S01-3B_G0041280.mRNA.1.CDS.1 / HN1_G0041880.mRNA.1.CDS.1 / JXXY16_1_G0041430.mRNA.1.CDS.1 / XXYS1_4_G0043840.mRNA.1.CDS.1 / Y55_G0041890.mRNA.1.CDS.1


Mass: 13942.640 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A7I9BRN0
#39: Protein Ribosomal protein L37


Mass: 9877.395 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1BIE7
#40: Protein BJ4_G0032190.mRNA.1.CDS.1 / BJ4_G0038170.mRNA.1.CDS.1 / EM14S01-3B_G0039190.mRNA.1.CDS.1 / HLJ1_G0029860.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0038170.mRNA.1.CDS.1 / EM14S01-3B_G0039190.mRNA.1.CDS.1 / HLJ1_G0029860.mRNA.1.CDS.1 / HLJ1_G0038220.mRNA.1.CDS.1 / HN1_G0029640.mRNA.1.CDS.1 / JXXY16_1_G0039350.mRNA.1.CDS.1 / SX2_G0029560.mRNA.1.CDS.1 / XXYS1_4_G0041730.mRNA.1.CDS.1 / Y55_G0029760.mRNA.1.CDS.1


Mass: 8845.561 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5PUL8
#42: Protein Ubiquitin


Mass: 14583.077 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6V8S9K0
#44: Protein BJ4_G0001880.mRNA.1.CDS.1 / BJ4_G0013440.mRNA.1.CDS.1 / HLJ1_G0010810.mRNA.1.CDS.1 / HLJ1_G0056520.mRNA.1.CDS.1 / HN1_G0010910. ...BJ4_G0013440.mRNA.1.CDS.1 / HLJ1_G0010810.mRNA.1.CDS.1 / HLJ1_G0056520.mRNA.1.CDS.1 / HN1_G0010910.mRNA.1.CDS.1 / HN1_G0056250.mRNA.1.CDS.1 / JXXY16_1_G0010600.mRNA.1.CDS.1 / SX2_G0010840.mRNA.1.CDS.1 / XXYS1_4_G0001900.mRNA.1.CDS.1 / Y55_G0011070.mRNA.1.CDS.1


Mass: 12246.658 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1B5X0
#50: Protein BJ4_G0045400.mRNA.1.CDS.1


Mass: 26542.789 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0ZVK6
#52: Protein Rps5p


Mass: 25072.600 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A1L4AA68
#56: Protein BJ4_G0026100.mRNA.1.CDS.1 / EM14S01-3B_G0018500.mRNA.1.CDS.1 / HLJ1_G0023450.mRNA.1.CDS.1 / HN1_G0023480.mRNA.1.CDS.1 / JXXY16_ ...EM14S01-3B_G0018500.mRNA.1.CDS.1 / HLJ1_G0023450.mRNA.1.CDS.1 / HN1_G0023480.mRNA.1.CDS.1 / JXXY16_1_G0018540.mRNA.1.CDS.1 / XXYS1_4_G0021040.mRNA.1.CDS.1 / Y55_G0019160.mRNA.1.CDS.1


Mass: 22487.893 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A7I9GPQ3
#63: Protein BJ4_G0008010.mRNA.1.CDS.1 / BJ4_G0045230.mRNA.1.CDS.1 / EM14S01-3B_G0042280.mRNA.1.CDS.1 / HLJ1_G0007850.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0045230.mRNA.1.CDS.1 / EM14S01-3B_G0042280.mRNA.1.CDS.1 / HLJ1_G0007850.mRNA.1.CDS.1 / HLJ1_G0042070.mRNA.1.CDS.1 / HLJ1_G0043020.mRNA.1.CDS.1 / JXXY16_1_G0023670.mRNA.1.CDS.1 / SX2_G0024190.mRNA.1.CDS.1 / XXYS1_4_G0026180.mRNA.1.CDS.1 / Y55_G0042870.mRNA.1.CDS.1


Mass: 15877.490 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0YQ58
#64: Protein BJ4_G0020710.mRNA.1.CDS.1 / BJ4_G0040570.mRNA.1.CDS.1 / EM14S01-3B_G0022060.mRNA.1.CDS.1 / HLJ1_G0018110.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0040570.mRNA.1.CDS.1 / EM14S01-3B_G0022060.mRNA.1.CDS.1 / HLJ1_G0018110.mRNA.1.CDS.1 / HLJ1_G0040530.mRNA.1.CDS.1 / HN1_G0018060.mRNA.1.CDS.1 / JXXY16_1_G0022100.mRNA.1.CDS.1 / SX2_G0022620.mRNA.1.CDS.1 / XXYS1_4_G0024670.mRNA.1.CDS.1 / Y55_G0022810.mRNA.1.CDS.1


Mass: 15820.413 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1BLL7
#75: Protein eS28


Mass: 7605.847 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5Q700
#76: Protein HLJ1_G0030400.mRNA.1.CDS.1 / HLJ1_G0038740.mRNA.1.CDS.1 / HN1_G0030180.mRNA.1.CDS.1 / JXXY16_1_G0039900.mRNA.1.CDS.1 / SX2_ ...HLJ1_G0038740.mRNA.1.CDS.1 / HN1_G0030180.mRNA.1.CDS.1 / JXXY16_1_G0039900.mRNA.1.CDS.1 / SX2_G0030120.mRNA.1.CDS.1 / XXYS1_4_G0042270.mRNA.1.CDS.1 / Y55_G0030330.mRNA.1.CDS.1


Mass: 6675.723 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5PV92
#78: Protein BJ4_G0022010.mRNA.1.CDS.1 / BJ4_G0041890.mRNA.1.CDS.1 / EM14S01-3B_G0023350.mRNA.1.CDS.1 / Guanine nucleotide-binding protein ...BJ4_G0041890.mRNA.1.CDS.1 / EM14S01-3B_G0023350.mRNA.1.CDS.1 / Guanine nucleotide-binding protein subunit beta-like protein / HLJ1_G0019440.mRNA.1.CDS.1 / HN1_G0019400.mRNA.1.CDS.1 / JXXY16_1_G0023400.mRNA.1.CDS.1 / SX2_G0023940.mRNA.1.CDS.1 / XXYS1_4_G0025910.mRNA.1.CDS.1


Mass: 34841.219 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1BKH8
#79: Protein Ubiquitin-40S ribosomal protein S31


Mass: 17254.227 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5PU37
#80: Protein Elongation factor 2


Mass: 93407.125 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5Q7K2

+
60S ribosomal protein ... , 27 types, 27 molecules uL2uL3uL18eL6uL30eL8uL6uL16eL13eL14eL15uL13eL19eL20eL22uL14eL24uL23uL24eL27uL15eL29eL34eL36eL39eL43uL11

#5: Protein 60S ribosomal protein L2-B / BJ4_G0017640.mRNA.1.CDS.1 / BJ4_G0052790.mRNA.1.CDS.1 / BJ4_G0056090.mRNA.1.CDS.1 / EM14S01-3B_ ...BJ4_G0017640.mRNA.1.CDS.1 / BJ4_G0052790.mRNA.1.CDS.1 / BJ4_G0056090.mRNA.1.CDS.1 / EM14S01-3B_G0049790.mRNA.1.CDS.1 / HLJ1_G0053940.mRNA.1.CDS.1 / HN1_G0053700.mRNA.1.CDS.1 / JXXY16_1_G0053260.mRNA.1.CDS.1 / SX2_G0053690.mRNA.1.CDS.1 / XXYS1_4_G0055700.mRNA.1.CDS.1


Mass: 27463.574 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0Y8L2
#6: Protein 60S ribosomal protein L3 / BJ4_G0035580.mRNA.1.CDS.1 / BJ4_G0049970.mRNA.1.CDS.1 / EM14S01-3B_G0027500.mRNA.1.CDS.1 / HN1_ ...BJ4_G0035580.mRNA.1.CDS.1 / BJ4_G0049970.mRNA.1.CDS.1 / EM14S01-3B_G0027500.mRNA.1.CDS.1 / HN1_G0038260.mRNA.1.CDS.1 / JXXY16_1_G0027670.mRNA.1.CDS.1 / XXYS1_4_G0030010.mRNA.1.CDS.1 / Y55_G0038280.mRNA.1.CDS.1


Mass: 43850.793 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5Q627
#8: Protein 60S ribosomal protein L5 / BJ4_G0025610.mRNA.1.CDS.1 / BJ4_G0054540.mRNA.1.CDS.1 / HLJ1_G0022950.mRNA.1.CDS.1 / Y55_G0018660.mRNA.1.CDS.1


Mass: 33764.828 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5Q0W8
#9: Protein 60S ribosomal protein L6


Mass: 20000.564 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1BLT3
#10: Protein 60S ribosomal protein L7-A / L6 / Large ribosomal subunit protein uL30-A / RP11 / YL8


Mass: 27686.281 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05737
#11: Protein 60S ribosomal protein L8-A / L4 / L4-2 / L7a-1 / Large ribosomal subunit protein eL8-A / Maintenance of killer protein 7 / RP6 / YL5


Mass: 28175.820 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P17076
#12: Protein 60S ribosomal protein L9-A / L8 / Large ribosomal subunit protein uL6-A / RP24 / YL11


Mass: 21605.061 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05738
#13: Protein 60S ribosomal protein L10 / BJ4_G0029950.mRNA.1.CDS.1 / BJ4_G0035810.mRNA.1.CDS.1 / EM14S01-3B_G0037000.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0029950.mRNA.1.CDS.1 / BJ4_G0035810.mRNA.1.CDS.1 / EM14S01-3B_G0037000.mRNA.1.CDS.1 / HLJ1_G0027670.mRNA.1.CDS.1 / HLJ1_G0035970.mRNA.1.CDS.1 / HN1_G0027430.mRNA.1.CDS.1 / JXXY16_1_G0037140.mRNA.1.CDS.1 / SX2_G0027370.mRNA.1.CDS.1 / XXYS1_4_G0039550.mRNA.1.CDS.1


Mass: 25410.465 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5PUZ5
#15: Protein 60S ribosomal protein L13


Mass: 22604.164 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1AF71
#16: Protein 60S ribosomal protein L14-A / Large ribosomal subunit protein eL14-A


Mass: 15195.066 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P36105
#17: Protein 60S ribosomal protein L15-A / L13 / Large ribosomal subunit protein eL15-A / RP15R / YL10 / YP18


Mass: 24482.357 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05748
#18: Protein 60S ribosomal protein L16-A / BJ4_G0026140.mRNA.1.CDS.1 / BJ4_G0055070.mRNA.1.CDS.1 / EM14S01-3B_G0052010.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0026140.mRNA.1.CDS.1 / BJ4_G0055070.mRNA.1.CDS.1 / EM14S01-3B_G0052010.mRNA.1.CDS.1 / HLJ1_G0052810.mRNA.1.CDS.1 / HN1_G0052690.mRNA.1.CDS.1 / SX2_G0052670.mRNA.1.CDS.1 / XXYS1_4_G0054680.mRNA.1.CDS.1


Mass: 22247.227 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0ZD57
#21: Protein 60S ribosomal protein L19-A / L23 / Large ribosomal subunit protein eL19-A / RP15L / RP33 / YL14


Mass: 21762.316 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX82
#22: Protein 60S ribosomal protein L20


Mass: 20478.852 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6V8RR03
#24: Protein 60S ribosomal protein L22-A / BJ4_G0029820.mRNA.1.CDS.1 / BJ4_G0035690.mRNA.1.CDS.1 / EM14S01-3B_G0036880.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0029820.mRNA.1.CDS.1 / BJ4_G0035690.mRNA.1.CDS.1 / EM14S01-3B_G0036880.mRNA.1.CDS.1 / HLJ1_G0027540.mRNA.1.CDS.1 / HLJ1_G0035840.mRNA.1.CDS.1 / HN1_G0027300.mRNA.1.CDS.1 / JXXY16_1_G0037020.mRNA.1.CDS.1 / SX2_G0027240.mRNA.1.CDS.1 / Y55_G0027440.mRNA.1.CDS.1


Mass: 13711.359 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0ZZW6
#25: Protein 60S ribosomal protein L23-B / BJ4_G0015890.mRNA.1.CDS.1 / EM14S01-3B_G0012990.mRNA.1.CDS.1 / HLJ1_G0001130.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0015890.mRNA.1.CDS.1 / EM14S01-3B_G0012990.mRNA.1.CDS.1 / HLJ1_G0001130.mRNA.1.CDS.1 / HLJ1_G0013350.mRNA.1.CDS.1 / HN1_G0013410.mRNA.1.CDS.1 / JXXY16_1_G0012990.mRNA.1.CDS.1 / SX2_G0001980.mRNA.1.CDS.1 / Y55_G0001980.mRNA.1.CDS.1 / Y55_G0013520.mRNA.1.CDS.1


Mass: 14493.950 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0Z7E4
#26: Protein 60S ribosomal protein L24-A / BJ4_G0019990.mRNA.1.CDS.1 / BJ4_G0040660.mRNA.1.CDS.1 / EM14S01-3B_G0032620.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0019990.mRNA.1.CDS.1 / BJ4_G0040660.mRNA.1.CDS.1 / EM14S01-3B_G0032620.mRNA.1.CDS.1 / HLJ1_G0019730.mRNA.1.CDS.1 / HLJ1_G0033130.mRNA.1.CDS.1 / HN1_G0032980.mRNA.1.CDS.1 / SX2_G0038260.mRNA.1.CDS.1 / Y55_G0033090.mRNA.1.CDS.1


Mass: 17661.717 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5PY83
#27: Protein 60S ribosomal protein L25 / BJ4_G0033830.mRNA.1.CDS.1 / HLJ1_G0036700.mRNA.1.CDS.1 / HLJ1_G0048120.mRNA.1.CDS.1 / HN1_G0036450. ...BJ4_G0033830.mRNA.1.CDS.1 / HLJ1_G0036700.mRNA.1.CDS.1 / HLJ1_G0048120.mRNA.1.CDS.1 / HN1_G0036450.mRNA.1.CDS.1 / SX2_G0031260.mRNA.1.CDS.1 / Y55_G0036470.mRNA.1.CDS.1


Mass: 15787.612 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0YSM0
#28: Protein 60S ribosomal protein L26-A / L33 / Large ribosomal subunit protein uL24-A / YL33


Mass: 14265.784 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05743
#29: Protein 60S ribosomal protein L27


Mass: 15437.166 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0ZQQ7
#30: Protein 60S ribosomal protein L28 / BJ4_G0019380.mRNA.1.CDS.1 / BJ4_G0040060.mRNA.1.CDS.1 / EM14S01-3B_G0031990.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0019380.mRNA.1.CDS.1 / BJ4_G0040060.mRNA.1.CDS.1 / EM14S01-3B_G0031990.mRNA.1.CDS.1 / HLJ1_G0019110.mRNA.1.CDS.1 / HLJ1_G0032520.mRNA.1.CDS.1 / HN1_G0032350.mRNA.1.CDS.1 / JXXY16_1_G0032170.mRNA.1.CDS.1 / SX2_G0037610.mRNA.1.CDS.1 / XXYS1_4_G0034590.mRNA.1.CDS.1


Mass: 16761.666 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0ZDK5
#31: Protein 60S ribosomal protein L29


Mass: 6691.884 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5PWR1
#36: Protein 60S ribosomal protein L34-A / BJ4_G0003940.mRNA.1.CDS.1 / BJ4_G0015190.mRNA.1.CDS.1 / EM14S01-3B_G0001280.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0003940.mRNA.1.CDS.1 / BJ4_G0015190.mRNA.1.CDS.1 / EM14S01-3B_G0001280.mRNA.1.CDS.1 / HLJ1_G0001330.mRNA.1.CDS.1 / HLJ1_G0014970.mRNA.1.CDS.1 / HN1_G0001300.mRNA.1.CDS.1 / SX2_G0001340.mRNA.1.CDS.1 / XXYS1_4_G0004140.mRNA.1.CDS.1 / Y55_G0001350.mRNA.1.CDS.1


Mass: 13673.196 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0ZFW3
#38: Protein 60S ribosomal protein L36-A / L39 / Large ribosomal subunit protein eL36-A / YL39


Mass: 11151.259 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P05745
#41: Protein 60S ribosomal protein L39


Mass: 6358.640 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1ASX0
#45: Protein 60S ribosomal protein L43-A / L37a / Large ribosomal subunit protein eL43-A / YL35


Mass: 10112.952 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P0CX25
#83: Protein 60S ribosomal protein L12-B / BJ4_G0002860.mRNA.1.CDS.1 / BJ4_G0014050.mRNA.1.CDS.1 / EM14S01-3B_G0000190.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0002860.mRNA.1.CDS.1 / BJ4_G0014050.mRNA.1.CDS.1 / EM14S01-3B_G0000190.mRNA.1.CDS.1 / HLJ1_G0000220.mRNA.1.CDS.1 / HLJ1_G0013810.mRNA.1.CDS.1 / HN1_G0000200.mRNA.1.CDS.1 / JXXY16_1_G0000240.mRNA.1.CDS.1 / XXYS1_4_G0003040.mRNA.1.CDS.1 / Y55_G0000220.mRNA.1.CDS.1


Mass: 17850.621 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5Q275

-
Protein/peptide , 1 types, 1 molecules eL41

#43: Protein/peptide eL41


Mass: 3354.243 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast)

+
40S ribosomal protein ... , 24 types, 24 molecules uS2eS1uS5eS4eS6eS7eS8eS10uS17eS12uS15uS11uS19uS13eS19uS10eS21uS8uS12eS24eS25eS26eS27eS30

#47: Protein 40S ribosomal protein S0


Mass: 28051.330 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0ZRP4
#48: Protein 40S ribosomal protein S1


Mass: 28798.467 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5PRY4
#49: Protein 40S ribosomal protein S2 / BJ4_G0039880.mRNA.1.CDS.1 / HLJ1_G0018940.mRNA.1.CDS.1 / HLJ1_G0032340.mRNA.1.CDS.1 / HN1_G0032170. ...BJ4_G0039880.mRNA.1.CDS.1 / HLJ1_G0018940.mRNA.1.CDS.1 / HLJ1_G0032340.mRNA.1.CDS.1 / HN1_G0032170.mRNA.1.CDS.1 / SX2_G0037420.mRNA.1.CDS.1 / Y55_G0032300.mRNA.1.CDS.1


Mass: 27490.826 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5PWF7
#51: Protein 40S ribosomal protein S4


Mass: 29469.330 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0Z3Q0
#53: Protein 40S ribosomal protein S6


Mass: 27054.486 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1AMY1
#54: Protein 40S ribosomal protein S7


Mass: 21658.209 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1ACT7
#55: Protein 40S ribosomal protein S8


Mass: 22537.803 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5Q1H1
#57: Protein 40S ribosomal protein S10-A / BJ4_G0052110.mRNA.1.CDS.1 / EM14S01-3B_G0029550.mRNA.1.CDS.1 / HLJ1_G0040520.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0052110.mRNA.1.CDS.1 / EM14S01-3B_G0029550.mRNA.1.CDS.1 / HLJ1_G0040520.mRNA.1.CDS.1 / HLJ1_G0052080.mRNA.1.CDS.1 / HN1_G0040350.mRNA.1.CDS.1 / JXXY16_1_G0029740.mRNA.1.CDS.1 / SX2_G0035120.mRNA.1.CDS.1 / XXYS1_4_G0032110.mRNA.1.CDS.1 / Y55_G0040350.mRNA.1.CDS.1


Mass: 12757.445 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0ZM59
#58: Protein 40S ribosomal protein S11-B / BJ4_G0008990.mRNA.1.CDS.1 / BJ4_G0017130.mRNA.1.CDS.1 / EM14S01-3B_G0043020.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0008990.mRNA.1.CDS.1 / BJ4_G0017130.mRNA.1.CDS.1 / EM14S01-3B_G0043020.mRNA.1.CDS.1 / HLJ1_G0008760.mRNA.1.CDS.1 / HLJ1_G0014680.mRNA.1.CDS.1 / HLJ1_G0043980.mRNA.1.CDS.1 / JXXY16_1_G0014240.mRNA.1.CDS.1 / SX2_G0014590.mRNA.1.CDS.1 / Y55_G0014780.mRNA.1.CDS.1


Mass: 17785.934 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0YMX6
#59: Protein 40S ribosomal protein S12


Mass: 15488.631 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5Q8I3
#60: Protein 40S ribosomal protein S13 / BJ4_G0046510.mRNA.1.CDS.1 / HLJ1_G0009120.mRNA.1.CDS.1 / HLJ1_G0044330.mRNA.1.CDS.1 / HN1_G0044100. ...BJ4_G0046510.mRNA.1.CDS.1 / HLJ1_G0009120.mRNA.1.CDS.1 / HLJ1_G0044330.mRNA.1.CDS.1 / HN1_G0044100.mRNA.1.CDS.1 / SX2_G0044200.mRNA.1.CDS.1 / Y55_G0044190.mRNA.1.CDS.1


Mass: 17059.945 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L0YFM5
#61: Protein 40S ribosomal protein S14-A / BJ4_G0005840.mRNA.1.CDS.1 / BJ4_G0054020.mRNA.1.CDS.1 / EM14S01-3B_G0050970.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0005840.mRNA.1.CDS.1 / BJ4_G0054020.mRNA.1.CDS.1 / EM14S01-3B_G0050970.mRNA.1.CDS.1 / HLJ1_G0051820.mRNA.1.CDS.1 / HN1_G0051650.mRNA.1.CDS.1 / JXXY16_1_G0051180.mRNA.1.CDS.1 / SX2_G0051630.mRNA.1.CDS.1 / XXYS1_4_G0053620.mRNA.1.CDS.1 / Y55_G0051740.mRNA.1.CDS.1


Mass: 14562.655 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1AAG7
#62: Protein 40S ribosomal protein S15 / BJ4_G0034640.mRNA.1.CDS.1 / EM14S01-3B_G0026550.mRNA.1.CDS.1 / HLJ1_G0037510.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0034640.mRNA.1.CDS.1 / EM14S01-3B_G0026550.mRNA.1.CDS.1 / HLJ1_G0037510.mRNA.1.CDS.1 / HLJ1_G0048960.mRNA.1.CDS.1 / HN1_G0037290.mRNA.1.CDS.1 / JXXY16_1_G0026690.mRNA.1.CDS.1 / SX2_G0032080.mRNA.1.CDS.1 / XXYS1_4_G0029070.mRNA.1.CDS.1 / Y55_G0037310.mRNA.1.CDS.1


Mass: 16031.907 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5Q6F2
#65: Protein 40S ribosomal protein S18-B / BJ4_G0020690.mRNA.1.CDS.1 / BJ4_G0040550.mRNA.1.CDS.1 / EM14S01-3B_G0047090.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0020690.mRNA.1.CDS.1 / BJ4_G0040550.mRNA.1.CDS.1 / EM14S01-3B_G0047090.mRNA.1.CDS.1 / HLJ1_G0012740.mRNA.1.CDS.1 / HLJ1_G0047970.mRNA.1.CDS.1 / JXXY16_1_G0047210.mRNA.1.CDS.1 / SX2_G0047790.mRNA.1.CDS.1 / XXYS1_4_G0049690.mRNA.1.CDS.1 / Y55_G0022790.mRNA.1.CDS.1


Mass: 17071.641 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1AIT6
#66: Protein 40S ribosomal protein S19-A / BJ4_G0033890.mRNA.1.CDS.1 / EM14S01-3B_G0025820.mRNA.1.CDS.1 / HLJ1_G0036760.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0033890.mRNA.1.CDS.1 / EM14S01-3B_G0025820.mRNA.1.CDS.1 / HLJ1_G0036760.mRNA.1.CDS.1 / HLJ1_G0048180.mRNA.1.CDS.1 / HN1_G0036510.mRNA.1.CDS.1 / JXXY16_1_G0025950.mRNA.1.CDS.1 / SX2_G0031320.mRNA.1.CDS.1 / XXYS1_4_G0028360.mRNA.1.CDS.1 / Y55_G0036530.mRNA.1.CDS.1


Mass: 15942.125 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1BWT3
#67: Protein 40S ribosomal protein S20 / BJ4_G0000330.mRNA.1.CDS.1 / BJ4_G0023380.mRNA.1.CDS.1 / EM14S01-3B_G0053970.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0000330.mRNA.1.CDS.1 / BJ4_G0023380.mRNA.1.CDS.1 / EM14S01-3B_G0053970.mRNA.1.CDS.1 / HLJ1_G0023150.mRNA.1.CDS.1 / HLJ1_G0054920.mRNA.1.CDS.1 / HN1_G0054660.mRNA.1.CDS.1 / SX2_G0054700.mRNA.1.CDS.1 / Y55_G0054930.mRNA.1.CDS.1


Mass: 13929.044 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5PVE9
#68: Protein 40S ribosomal protein S21


Mass: 9758.829 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1BDN3
#69: Protein 40S ribosomal protein S22-A / BJ4_G0008720.mRNA.1.CDS.1 / EM14S01-3B_G0005800.mRNA.1.CDS.1 / HLJ1_G0006100.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0008720.mRNA.1.CDS.1 / EM14S01-3B_G0005800.mRNA.1.CDS.1 / HLJ1_G0006100.mRNA.1.CDS.1 / HLJ1_G0028000.mRNA.1.CDS.1 / HN1_G0006170.mRNA.1.CDS.1 / JXXY16_1_G0005950.mRNA.1.CDS.1 / SX2_G0006150.mRNA.1.CDS.1 / XXYS1_4_G0008600.mRNA.1.CDS.1 / Y55_G0006330.mRNA.1.CDS.1


Mass: 14650.062 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5PSE9
#70: Protein 40S ribosomal protein S23 / BJ4_G0028020.mRNA.1.CDS.1 / EM14S01-3B_G0020410.mRNA.1.CDS.1 / HLJ1_G0021090.mRNA.1.CDS.1 / HLJ1_ ...BJ4_G0028020.mRNA.1.CDS.1 / EM14S01-3B_G0020410.mRNA.1.CDS.1 / HLJ1_G0021090.mRNA.1.CDS.1 / HLJ1_G0025380.mRNA.1.CDS.1 / HLJ1_G0034480.mRNA.1.CDS.1 / HN1_G0025390.mRNA.1.CDS.1 / HN1_G0034320.mRNA.1.CDS.1 / JXXY16_1_G0034150.mRNA.1.CDS.1 / SX2_G0039600.mRNA.1.CDS.1


Mass: 16073.896 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1B1T2
#71: Protein 40S ribosomal protein S24


Mass: 15362.848 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1AUB6
#72: Protein 40S ribosomal protein S25


Mass: 12067.272 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5PZ18
#73: Protein 40S ribosomal protein S26


Mass: 13538.922 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6A5PUG0
#74: Protein 40S ribosomal protein S27-A / RP61 / Small ribosomal subunit protein eS27-A / YS20


Mass: 8893.391 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P35997
#77: Protein 40S ribosomal protein S30


Mass: 7137.541 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: A0A6L1A1E1

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Non-polymers , 3 types, 499 molecules

#84: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 490 / Source method: obtained synthetically / Formula: Mg
#85: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Zn
#86: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.03 Å3/Da / Density % sol: 59.43 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: PEG10K, KSCN, TRIS-HAC

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: NSLS / Beamline: X6A / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Aug 8, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 3→154.68 Å / Num. obs: 734615 / % possible obs: 99.98 % / Redundancy: 206 % / Biso Wilson estimate: 112.7 Å2 / CC1/2: 0.99 / Net I/σ(I): 20.12
Reflection shellResolution: 3→3.1 Å / Mean I/σ(I) obs: 0.95 / Num. unique obs: 72990 / CC1/2: 0.23

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Processing

Software
NameVersionClassification
PHENIX1.16_3549refinement
XDSdata reduction
XSCALEdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4V88
Resolution: 3→154.68 Å / SU ML: 0.5314 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.8096
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2504 1796 0.24 %
Rwork0.1958 732713 -
obs0.196 734509 99.98 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 119.97 Å2
Refinement stepCycle: LAST / Resolution: 3→154.68 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms95269 112255 530 0 208054
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0084222565
X-RAY DIFFRACTIONf_angle_d1.307326167
X-RAY DIFFRACTIONf_chiral_restr0.05740823
X-RAY DIFFRACTIONf_plane_restr0.00821900
X-RAY DIFFRACTIONf_dihedral_angle_d17.9266100112
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3-3.080.41261370.351255929X-RAY DIFFRACTION100
3.08-3.170.34861380.287956031X-RAY DIFFRACTION100
3.17-3.270.3151360.264156039X-RAY DIFFRACTION100
3.27-3.390.29691390.239756130X-RAY DIFFRACTION100
3.39-3.530.31411360.226556108X-RAY DIFFRACTION100
3.53-3.690.25951390.220256165X-RAY DIFFRACTION100
3.69-3.880.27271370.206956133X-RAY DIFFRACTION100
3.88-4.130.26431380.189456285X-RAY DIFFRACTION100
4.13-4.440.24031380.182656292X-RAY DIFFRACTION100
4.44-4.890.20411380.171356375X-RAY DIFFRACTION100
4.89-5.60.24141390.170456553X-RAY DIFFRACTION100
5.6-7.050.23261390.196556855X-RAY DIFFRACTION100
7.05-154.680.23851420.183757818X-RAY DIFFRACTION99.79

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