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Yorodumi- PDB-7osm: Intermediate translocation complex of 80 S.cerevisiae ribosome wi... -
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Open data
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Basic information
| Entry | Database: PDB / ID: 7osm | ||||||
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| Title | Intermediate translocation complex of 80 S.cerevisiae ribosome with eEF2 and ligands | ||||||
Components |
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Keywords | RIBOSOME / translocation / eEF2 / tRNA / mRNA / eukaryotic ribosome | ||||||
| Function / homology | Function and homology informationribosomal subunit / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression ...ribosomal subunit / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / translation elongation factor activity / protein-RNA complex assembly / maturation of LSU-rRNA / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / macroautophagy / modification-dependent protein catabolic process / protein tag activity / rRNA processing / large ribosomal subunit / ribosome biogenesis / ribosome binding / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / protein ubiquitination / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / GTPase activity / mRNA binding / ubiquitin protein ligase binding / GTP binding / nucleolus / RNA binding / zinc ion binding / metal ion binding / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
| Biological species | ![]() ![]() | ||||||
| Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å | ||||||
Authors | Djumagulov, M. / Jenner, L. / Rozov, A. / Demeshkina, N. / Yusupov, M. / Yusupova, G. | ||||||
Citation | Journal: Nature / Year: 2021Title: Accuracy mechanism of eukaryotic ribosome translocation. Authors: Djumagulov, M. / Demeshkina, N. / Jenner, L. / Rozov, A. / Yusupov, M. / Yusupova, G. #1: Journal: Acta Crystallogr., Sect. D: Biol. Crystallogr. / Year: 2012Title: Towards automated crystallographic structure refinement with phenix.refine. Authors: Adams, P.D. | ||||||
| History |
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Structure visualization
| Structure viewer | Molecule: Molmil Jmol/JSmol |
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Downloads & links
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Download
| PDBx/mmCIF format | 7osm.cif.gz | 5.2 MB | Display | PDBx/mmCIF format |
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| PDB format | pdb7osm.ent.gz | Display | PDB format | |
| PDBx/mmJSON format | 7osm.json.gz | Tree view | PDBx/mmJSON format | |
| Others | Other downloads |
-Validation report
| Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/os/7osm ftp://data.pdbj.org/pub/pdb/validation_reports/os/7osm | HTTPS FTP |
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-Related structure data
| Related structure data | ![]() 7osaC ![]() 4v88S C: citing same article ( S: Starting model for refinement |
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| Similar structure data |
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Links
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Assembly
| Deposited unit | ![]()
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| Unit cell |
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Components
-RNA chain , 7 types, 7 molecules 25SAB58S18SASITPSITmRNA
| #1: RNA chain | Mass: 1097492.875 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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| #2: RNA chain | Mass: 38951.105 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #3: RNA chain | Mass: 50682.922 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #46: RNA chain | Mass: 579126.562 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #80: RNA chain | Mass: 24726.779 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #81: RNA chain | Mass: 24801.801 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
| #82: RNA chain | Mass: 2511.545 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() |
+Protein , 25 types, 25 molecules uL10uL4uL5uL22eL18eL21eL30eL31eL32eL33uL29eL37eL38eL40eL42uS3uS7uS4uS9eS17eS28uS14RACKeS31eEF2
+60S ribosomal protein ... , 27 types, 27 molecules uL2uL3uL18eL6uL30eL8uL6uL16eL13eL14eL15uL13eL19eL20eL22uL14eL24uL23uL24eL27uL15eL29eL34eL36eL39eL43uL11
-Protein/peptide , 1 types, 1 molecules eL41
| #43: Protein/peptide | Mass: 3354.243 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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+40S ribosomal protein ... , 23 types, 23 molecules uS2eS1uS5eS4eS6eS7eS8eS10uS17uS15uS11uS19uS13eS19uS10eS21uS8uS12eS24eS25eS26eS27eS30
-Non-polymers , 3 types, 508 molecules 




| #84: Chemical | ChemComp-MG / #85: Chemical | ChemComp-ZN / #86: Chemical | ChemComp-GTP / | |
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-Details
| Has ligand of interest | Y |
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-Experimental details
-Experiment
| Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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Sample preparation
| Crystal | Density Matthews: 2.74 Å3/Da / Density % sol: 55.05 % |
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| Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: PEG 6000, KSCN, Tris-HAc |
-Data collection
| Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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| Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X6A / Wavelength: 1 Å |
| Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Aug 8, 2019 |
| Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
| Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
| Reflection | Resolution: 3→149.93 Å / Num. obs: 712272 / % possible obs: 99.99 % / Redundancy: 172 % / Biso Wilson estimate: 105.49 Å2 / CC1/2: 0.99 / Net I/σ(I): 13.35 |
| Reflection shell | Resolution: 3.2→3.314 Å / Mean I/σ(I) obs: 0.94 / Num. unique obs: 58317 / CC1/2: 0.14 |
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Processing
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| Refinement | Method to determine structure: MOLECULAR REPLACEMENTStarting model: 4V88 Resolution: 3→149.93 Å / SU ML: 0.8588 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 37.5857 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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| Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Displacement parameters | Biso mean: 120.73 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Refinement step | Cycle: LAST / Resolution: 3→149.93 Å
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| Refine LS restraints |
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| LS refinement shell |
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