PDB-7n7s: Crystal Structure of Hydroxymethylglutaryl-CoA reductase from Eli...

ID or keywords:

Entry

Database: PDB / ID: 7n7s

Title

Crystal Structure of Hydroxymethylglutaryl-CoA reductase from Elizabethkingia anophelis NUHP1

Components

Hydroxymethylglutaryl-CoA reductase

Keywords

OXIDOREDUCTASE / SSGCID / hydroxymethylglutaryl-CoA reductase / HGM-CoA reductase / Elizabethkingia anophelis / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease

Function / homology

Function and homology information

hydroxymethylglutaryl-CoA reductase (NADPH) activity / coenzyme A metabolic process
Similarity search - Function

Biological species

Elizabethkingia anophelis NUHP1 (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.4 Å

Authors

Seattle Structural Genomics Center for Infectious Disease / Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Citation

Journal: To be published
Title: Crystal Structure of Hydroxymethylglutaryl-CoA reductase from Elizabethkingia anophelis NUHP1
Authors: Abendroth, J. / Lorimer, D.D. / Horanyi, P.S. / Edwards, T.E.

History

Deposition	Jun 11, 2021	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Jun 23, 2021	Provider: repository / Type: Initial release
Revision 1.1	Apr 3, 2024	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	7n7s.cif.gz	1.1 MB	Display	PDBx/mmCIF format
PDB format	pdb7n7s.ent.gz	800.3 KB	Display	PDB format
PDBx/mmJSON format	7n7s.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	7n7s_validation.pdf.gz	533.2 KB	Display	wwPDB validaton report
Full document	7n7s_full_validation.pdf.gz	546 KB	Display
Data in XML	7n7s_validation.xml.gz	93.1 KB	Display
Data in CIF	7n7s_validation.cif.gz	133.8 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/n7/7n7s ftp://data.pdbj.org/pub/pdb/validation_reports/n7/7n7s	HTTPS FTP

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Related structure data

Similar structure data	6tp3-d 1w7c-d 6tos-d 3pgb-d 1rky-d 7k0k-d 6tq7-d 2w54-2 6uek-2 6tp6-d Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

Similar structure data

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#96 - Dec 2007 Oxidosqualene Cyclase similarity (8)

Deposited unit

A: Hydroxymethylglutaryl-CoA reductase

B: Hydroxymethylglutaryl-CoA reductase

C: Hydroxymethylglutaryl-CoA reductase

D: Hydroxymethylglutaryl-CoA reductase

E: Hydroxymethylglutaryl-CoA reductase

F: Hydroxymethylglutaryl-CoA reductase

hetero molecules

311 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Hydroxymethylglutaryl-CoA reductase

B: Hydroxymethylglutaryl-CoA reductase

hetero molecules

defined by author&software
Evidence: gel filtration
104 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

C: Hydroxymethylglutaryl-CoA reductase

E: Hydroxymethylglutaryl-CoA reductase

hetero molecules

defined by author&software
104 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

D: Hydroxymethylglutaryl-CoA reductase

F: Hydroxymethylglutaryl-CoA reductase

hetero molecules

defined by author&software
103 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	183.850, 206.670, 90.400
Angle α, β, γ (deg.)	90.000, 90.000, 90.000
Int Tables number	18
Space group name H-M	P2₁2₁2
Space group name Hall	P22ab
Symmetry operation	#1: x,y,z #2: x+1/2,-y+1/2,-z #3: -x+1/2,y+1/2,-z #4: -x,-y,z

#1: Protein	Hydroxymethylglutaryl-CoA reductase Mass: 50875.684 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Elizabethkingia anophelis NUHP1 (bacteria) Gene: BD94_0681 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: A0A077EA44
#2: Chemical	ChemComp-CIT / CITRIC ACID Mass: 192.124 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C₆H₈O₇
#3: Chemical	... ChemComp-SO4 / SULFATE ION Mass: 96.063 Da / Num. of mol.: 44 / Source method: obtained synthetically / Formula: SO₄
#4: Chemical	ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL Mass: 62.068 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₂H₆O₂
#5: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 1222 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.82 Å³/Da / Density % sol: 56.4 %
Crystal grow	Temperature: 287 K / Method: vapor diffusion, sitting drop / pH: 5.6 Details: Anatrace / Calibre Top96 screen, condition F1: 200 mM potassium sodium tartrate, 100 mM sodium citrate / citric acid pH 5.6, 2 M ammonium sulphate: ElanA.19063.a.B1.PS38245 at 23.3 mg/ml: ...Details: Anatrace / Calibre Top96 screen, condition F1: 200 mM potassium sodium tartrate, 100 mM sodium citrate / citric acid pH 5.6, 2 M ammonium sulphate: ElanA.19063.a.B1.PS38245 at 23.3 mg/ml: tray 290979 F1: cryo: 25% EG: puck ywm0-9.

Crystal

Density Matthews: 2.82 Å³/Da / Density % sol: 56.4 %

Crystal grow

Temperature: 287 K / Method: vapor diffusion, sitting drop / pH: 5.6
Details: Anatrace / Calibre Top96 screen, condition F1: 200 mM potassium sodium tartrate, 100 mM sodium citrate / citric acid pH 5.6, 2 M ammonium sulphate: ElanA.19063.a.B1.PS38245 at 23.3 mg/ml: ...

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Data collection

Diffraction

Mean temperature: 100 K / Serial crystal experiment: N

Diffraction source

Source:

SYNCHROTRON / Site:

APS

/ Beamline: 21-ID-F / Wavelength: 0.97872 Å

Detector

Type: RAYONIX MX-300 / Detector: CCD / Date: Feb 7, 2019 / Details: Beryllium Lenses

Radiation

Monochromator: Diamond [111] / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.97872 Å / Relative weight: 1

Reflection

Resolution: 2.4→50 Å / Num. obs: 135029 / % possible obs: 100 % / Redundancy: 8.357 % / B_iso Wilson estimate: 45.851 Å² / CC_1/2: 0.999 / R_merge(I) obs: 0.079 / R_rim(I) all: 0.085 / Χ²: 1.003 / Net I/σ(I): 19.24

Reflection shell

Diffraction-ID: 1

Resolution (Å)	Redundancy (%)	R_merge(I) obs	Mean I/σ(I) obs	Num. unique obs	CC_1/2	R_rim(I) all	% possible all
2.4-2.46	8.453	0.56	3.92	9870	0.899	0.596	100
2.46-2.53	8.449	0.446	4.88	9639	0.933	0.475	100
2.53-2.6	8.437	0.386	5.62	9384	0.949	0.411	100
2.6-2.68	8.435	0.342	6.37	9130	0.959	0.365	100
2.68-2.77	8.424	0.26	8.22	8829	0.976	0.277	100
2.77-2.87	8.422	0.214	9.94	8539	0.984	0.228	100
2.87-2.98	8.399	0.177	11.79	8300	0.988	0.189	100
2.98-3.1	8.401	0.144	14.15	7942	0.992	0.153	100
3.1-3.24	8.368	0.109	18.05	7661	0.995	0.116	100
3.24-3.39	8.381	0.086	22.16	7294	0.996	0.092	100
3.39-3.58	8.37	0.072	25.86	6965	0.998	0.077	100
3.58-3.79	8.367	0.061	29.44	6639	0.998	0.065	99.9
3.79-4.06	8.355	0.053	33.38	6194	0.998	0.056	100
4.06-4.38	8.346	0.045	37.9	5812	0.999	0.048	100
4.38-4.8	8.312	0.042	39.91	5377	0.999	0.045	100
4.8-5.37	8.281	0.042	39.71	4872	0.999	0.045	100
5.37-6.2	8.223	0.045	37.98	4314	0.999	0.048	100
6.2-7.59	8.117	0.042	39.89	3707	0.999	0.045	100
7.59-10.73	7.91	0.036	46.78	2901	0.999	0.039	100
10.73-50	7.081	0.036	45.53	1660	0.999	0.039	97.8

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Processing

Software

Name	Version	Classification
XDS		data reduction
XSCALE		data scaling
PHENIX	1.19 4224	refinement
PDB_EXTRACT	3.27	data extraction
MoRDa		phasing
PHENIX		model building
Coot		model building

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: pdb entry 5wpjA as per Morda

Resolution: 2.4→49.26 Å / SU ML: 0.2048 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 18.2656
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.1876	1956	1.45 %	0
R_work	0.1532	133056	-	-
obs	0.1537	135012	99.97 %	-

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 45.48 Å²

Refinement step

Cycle: LAST / Resolution: 2.4→49.26 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	18621	0	302	1222	20145

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0052	19485
X-RAY DIFFRACTION	f_angle_d	0.7012	26461
X-RAY DIFFRACTION	f_chiral_restr	0.0466	2945
X-RAY DIFFRACTION	f_plane_restr	0.0045	3398
X-RAY DIFFRACTION	f_dihedral_angle_d	14.2806	7021

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.4-2.46	0.2126	122	0.1879	9418	X-RAY DIFFRACTION	99.98
2.46-2.53	0.2245	156	0.1788	9399	X-RAY DIFFRACTION	100
2.53-2.6	0.2036	133	0.1824	9420	X-RAY DIFFRACTION	99.99
2.6-2.68	0.2503	131	0.1867	9410	X-RAY DIFFRACTION	100
2.68-2.78	0.2179	141	0.1737	9388	X-RAY DIFFRACTION	100
2.78-2.89	0.2094	123	0.1743	9475	X-RAY DIFFRACTION	99.98
2.89-3.02	0.2336	139	0.1834	9429	X-RAY DIFFRACTION	100
3.02-3.18	0.2259	137	0.1853	9466	X-RAY DIFFRACTION	99.98
3.18-3.38	0.1934	155	0.1652	9462	X-RAY DIFFRACTION	99.99
3.38-3.64	0.2025	154	0.1573	9478	X-RAY DIFFRACTION	99.98
3.64-4.01	0.1791	150	0.1405	9504	X-RAY DIFFRACTION	99.96
4.01-4.59	0.1289	156	0.116	9561	X-RAY DIFFRACTION	99.98
4.59-5.78	0.1659	115	0.1245	9683	X-RAY DIFFRACTION	100
5.78-49.26	0.1784	144	0.1512	9963	X-RAY DIFFRACTION	99.74

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.47930112627	0.287466872917	-0.0953970501367	0.615108214132	-0.116831000148	1.14522355161	-0.00630825628739	0.0349639894655	0.245732771898	0.11746798842	0.0167415942798	-0.151239048209	-0.195152769305	0.219517786316	0.000879630657971	0.377019771031	-0.024069588812	-0.134609679306	0.401605761847	-0.0189401184813	0.443461324196	105.468142909	72.2463512864	48.2525842726
2	2.64197160414	0.728192649246	0.48273211692	3.80521984766	1.34202128442	2.16452891123	-0.10008422736	0.318291393299	-0.0719575658817	-0.0549984842455	0.197024078077	-0.281440122938	-0.0574861176686	0.434918266801	-0.0941821168028	0.210411759337	-0.0177942554264	-0.0449805858062	0.371169671048	-0.0142770750554	0.26434467526	95.2751688848	42.8583270232	27.3867148992
3	3.51989180303	-0.0404968980258	-0.102739576206	1.11116737706	0.108417524403	1.51176105524	-0.0182114615143	-0.230765441366	0.186804273893	0.071502168328	0.0253812826733	0.122338096347	-0.033205723213	-0.225373575388	-0.0236600237706	0.282461261706	-0.00803431382852	-0.10405668493	0.320059832637	-0.0251798830997	0.301105139809	84.5260752946	64.2687851178	52.4972295816
4	0.700111566855	-0.209708345376	0.209618588479	0.765631545608	-0.420161694281	1.18792911812	-0.0554052671059	-0.035877845402	0.10868288465	-0.0380728341641	0.102713954692	0.0267156431681	-0.0596115627694	-0.239736646141	-0.047828200397	0.35608385512	0.0572900510738	-0.036783403094	0.35190812945	0.090228218716	0.398598682931	35.1379357552	91.2225706732	10.4842161846
5	3.54100386208	-0.967553311593	-0.0484489578685	1.41659008601	0.687058325324	1.49638060487	0.0511174097098	0.202079638565	0.525868763048	-0.0619836901978	-0.10277376002	-0.213113098804	-0.267901239178	0.122332547194	0.0435194655146	0.433158127484	-0.054145391128	-0.110994396731	0.360766580735	0.132947177301	0.499695068425	71.6876530968	91.3281660524	17.4536390766
6	2.61604454307	-0.498134024491	-1.31116192025	1.00287717662	-0.543035366811	2.55120468445	-0.123857293562	0.068449089346	0.320246638859	0.0356466217846	0.150705492865	-0.0303213133545	0.164560400687	-0.0626552504305	-0.0319131182466	0.370338172329	0.0104283739784	-0.117098820579	0.297104540976	0.0213205026128	0.34149059202	58.345702568	83.0191681313	24.8489235172
7	1.78810328718	-0.935994440512	0.299965437143	2.77948264166	-0.430077865331	1.61751960643	-0.139590792318	-0.306966456715	-0.0639256772765	0.347381667737	0.201040052702	0.0854302615013	-0.0054901673836	-0.156989101157	-0.0467582325283	0.297900054086	0.0417799331349	-0.0690826989176	0.341838920796	0.0727358505514	0.297229905328	42.6045467029	81.1268223443	28.1899662139
8	0.999188400377	-0.185461205078	0.589380593516	0.233964361619	-0.0296088094264	0.597959626889	-0.0953723465566	-0.160928687237	0.267816614762	0.119548909267	0.040950921293	-0.105762726284	-0.166725328086	-0.00901965403673	0.0493511964848	0.408920211283	-0.0203891797761	-0.111757475758	0.351449679495	-0.0174182403798	0.436686091721	86.881817837	80.737605654	46.0882798061
9	2.41840209614	0.379793650253	0.773320834821	1.79388063282	0.442882077385	2.00470598012	-0.0584123855737	0.36643136931	0.0843210759466	-0.151775106389	0.0592228701501	-0.0479487615109	-0.0189063815267	0.0993891437068	-0.00926936816693	0.301079778728	-0.0305413321417	-0.115291477369	0.399330247637	0.054092671607	0.360680086204	95.804308977	71.0711290661	27.4186024
10	1.94289190412	0.819543155557	-1.01256493596	1.54833090013	-0.344816610376	1.80990698118	-0.120152501521	0.091620165906	-0.115690560688	0.068533071947	0.0961403305129	0.157794743692	0.262069493257	-0.187713431143	0.0324684801092	0.250257094806	0.02323565822	-0.0297620433467	0.190839492503	-0.0383059029854	0.26959434715	56.5105088542	12.7772790137	21.8329142322
11	1.91964020859	0.150540449265	0.360129345111	3.37594050406	1.87549906777	3.00072630514	0.0137597348772	-0.174320556094	-0.0986710308006	0.640274223917	-0.00342440262202	-0.118632039948	0.352221916094	0.100581412445	-0.0272724928926	0.356121641498	-0.0119194141835	-0.0439548996398	0.297499122714	0.0313844200609	0.213342766402	76.4054385434	33.7426979086	46.4223139537
12	1.10917160706	0.439147178765	-0.163037304657	3.10923808351	-0.113436321873	1.29899675669	-0.0431396600486	0.17147189286	0.0623856863378	-0.24508000501	0.0989295302123	0.00457313390291	-0.0378475090759	0.0773210442877	-0.0651835150915	0.216164289866	-0.0143816516871	-0.0235251810646	0.246392125084	-0.039012902058	0.231364345799	69.7371982318	29.7730102208	12.3868164412
13	0.504447724535	0.0172716450612	-0.0978005448175	1.03800296843	-0.100715392416	0.68995266786	-0.0422290836962	0.11137129906	-0.0785820802995	-0.115352767632	0.0679818402093	0.112140487319	0.0676432354543	-0.0259890789365	-0.0409348810436	0.227453360523	-0.0405751114471	-0.0348219578372	0.257629809083	-0.0311215673798	0.276337719241	52.8399092729	22.1269246243	15.1922328136
14	1.8925570687	0.732894213899	-0.337087338533	3.33046176802	-0.482174432397	1.73392346918	-0.103086308922	0.26262982958	0.0187352421102	-0.734330123248	0.135424630429	0.058318332717	0.00216166982319	-0.0374795343629	-0.0561772574857	0.371757751867	-0.00898491695832	-0.0390990667689	0.270547517777	0.00227707127273	0.234260196867	50.0434530955	43.5334306477	2.7654681823
15	2.18427487547	0.567776546495	0.046643489511	3.09748445163	0.00562398654083	4.36837361669	0.153613484407	-0.637055374568	0.0396013253977	1.03706962497	-0.19328324489	0.160829042143	0.511141202033	0.254162534091	0.00919891287209	0.6086199125	0.0319841890032	0.0906015176088	0.403662582718	-0.0129219460705	0.351640332742	45.9156264037	32.3779419959	45.5275869954
16	1.67505832062	0.471482119672	-0.129946903351	2.62660738839	0.254271753753	1.43374289474	0.05619314855	-0.182357423516	0.1642235732	0.247732744268	0.0334150365684	0.134437707591	-0.0816647542633	-0.0668289677366	-0.0853232173181	0.227366301181	0.0269852291194	0.0262814561159	0.211636527961	-0.00717258749182	0.238754481318	52.3997804928	31.227013936	31.2398781312
17	1.20447852945	1.0163379769	0.237281311287	2.13749465608	1.74137805247	2.06169761129	0.190460934283	-0.599668944998	-0.224938325683	0.824826776401	0.121800372178	-0.00312139076377	0.157813399698	-0.132731921662	-0.24032653105	0.51580163636	0.0250570301785	0.00648421717495	0.370360913356	-0.0167569438478	0.270206908486	57.0297252811	28.7464515351	47.3045242536
18	5.26599774117	-0.701893286239	0.497818146108	2.40182075269	2.24844563354	8.35862349902	-0.0916134614269	0.543503822635	-0.66663761659	-1.29005221842	-0.245779544416	0.107955855965	0.813038373139	-0.306072555573	0.328906439754	1.19044222606	-0.0835635652166	0.0114871051073	0.652691638385	-0.0928866728491	0.47479492664	75.4798563316	25.6886219568	72.070824643
19	0.121268109202	-0.150975587864	-0.0796632584099	1.3375561094	-0.424037911519	0.358683065941	-0.0736856875815	0.020573361712	0.0386497586574	0.0116839854403	0.0682245488692	0.167212789293	-0.0578339258302	-0.15459088953	0.00585859548302	0.274032232744	0.0299037156995	-0.0536593357524	0.35406636099	0.0595716529562	0.350981389891	33.5217579866	71.7437773393	17.2943067653
20	3.29184384169	-1.05421678839	0.472065734441	2.1581510499	-0.564488682482	1.66405542694	0.0637084509035	0.177389349883	0.156557583946	-0.207476632687	-0.114218382276	-0.293827405751	0.0779361509271	0.197436005166	0.0242783836411	0.332604585775	0.0163522540122	-0.0263950844224	0.245712561467	0.0951848726367	0.361848572067	51.2757794689	76.4588819768	3.69688400917

Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

ID	Refine TLS-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	chain 'D' and (resid 2 through 114 )	D	D	2 - 114	1 - 113
2	2	chain 'D' and (resid 115 through 235 )	D	D	115 - 235	114 - 234
3	3	chain 'D' and (resid 236 through 390 )	D	D	236 - 390	235 - 389
4	4	chain 'E' and (resid 2 through 114 )	E	E	2 - 114	1 - 113
5	5	chain 'E' and (resid 115 through 210 )	E	E	115 - 210	114 - 209
6	6	chain 'E' and (resid 211 through 256 )	E	E	211 - 256	210 - 255
7	7	chain 'E' and (resid 257 through 390 )	E	E	257 - 390	256 - 389
8	8	chain 'F' and (resid 2 through 235 )	F	F	2 - 235	1 - 234
9	9	chain 'F' and (resid 236 through 390 )	F	F	236 - 390	235 - 389
10	10	chain 'A' and (resid -3 through 114 )	A	A	-3 - 114	1 - 118
11	11	chain 'A' and (resid 115 through 235 )	A	A	115 - 235	119 - 239
12	12	chain 'A' and (resid 236 through 390 )	A	A	236 - 390	240 - 394
13	13	chain 'B' and (resid 2 through 156 )	B	L	2 - 156	1 - 155
14	14	chain 'B' and (resid 157 through 235 )	B	L	157 - 235	156 - 234
15	15	chain 'B' and (resid 236 through 256 )	B	L	236 - 256	235 - 255
16	16	chain 'B' and (resid 257 through 364 )	B	L	257 - 364	256 - 363
17	17	chain 'B' and (resid 365 through 407 )	B	L	365 - 407	364 - 406
18	18	chain 'B' and (resid 408 through 442 )	B	L	408 - 442	407 - 441
19	19	chain 'C' and (resid 2 through 235 )	C	U	2 - 235	1 - 234
20	20	chain 'C' and (resid 236 through 390 )	C	U	236 - 390	235 - 389

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