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- PDB-7n5v: ZBTB7A Zinc Finger Domain Bound to DNA Duplex Containing GGACCC (... -

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Basic information

Entry
Database: PDB / ID: 7n5v
TitleZBTB7A Zinc Finger Domain Bound to DNA Duplex Containing GGACCC (Oligo 20)
Components
  • DNA Strand I
  • DNA Strand II
  • Zinc finger and BTB domain-containing protein 7A
KeywordsDNA BINDING PROTEIN/DNA / zinc-finger domain / gene expression / DNA BINDING PROTEIN-DNA complex
Function / homology
Function and homology information


regulation of transcription regulatory region DNA binding / negative regulation of androgen receptor signaling pathway / DNA-dependent protein kinase complex / double-strand break repair via classical nonhomologous end joining / regulation of DNA-binding transcription factor activity / regulation of glycolytic process / nuclear androgen receptor binding / histone acetyltransferase binding / negative regulation of Notch signaling pathway / fat cell differentiation ...regulation of transcription regulatory region DNA binding / negative regulation of androgen receptor signaling pathway / DNA-dependent protein kinase complex / double-strand break repair via classical nonhomologous end joining / regulation of DNA-binding transcription factor activity / regulation of glycolytic process / nuclear androgen receptor binding / histone acetyltransferase binding / negative regulation of Notch signaling pathway / fat cell differentiation / erythrocyte maturation / SMAD binding / regulation of alternative mRNA splicing, via spliceosome / protein localization to nucleus / B cell differentiation / transcription corepressor binding / negative regulation of transforming growth factor beta receptor signaling pathway / DNA-binding transcription repressor activity, RNA polymerase II-specific / sequence-specific double-stranded DNA binding / chromatin organization / site of double-strand break / positive regulation of NF-kappaB transcription factor activity / regulation of apoptotic process / DNA-binding transcription factor binding / DNA-binding transcription factor activity, RNA polymerase II-specific / chromatin remodeling / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / DNA-templated transcription / regulation of transcription by RNA polymerase II / negative regulation of transcription by RNA polymerase II / DNA binding / nucleus / metal ion binding / cytoplasm
Similarity search - Function
BTB/POZ domain / BTB domain profile. / Zinc finger, C2H2 type / Broad-Complex, Tramtrack and Bric a brac / BTB/POZ domain / zinc finger / Zinc finger C2H2 type domain profile. / Zinc finger C2H2 superfamily / SKP1/BTB/POZ domain superfamily / Zinc finger C2H2 type domain signature. / Zinc finger C2H2-type
Similarity search - Domain/homology
DNA / DNA (> 10) / Zinc finger and BTB domain-containing protein 7A
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.08 Å
AuthorsHorton, J.R. / Ren, R. / Cheng, X.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM049245-23 United States
CitationJournal: J.Biol.Chem. / Year: 2023
Title: Structural basis for transcription factor ZBTB7A recognition of DNA and effects of ZBTB7A somatic mutations that occur in human acute myeloid leukemia.
Authors: Ren, R. / Horton, J.R. / Chen, Q. / Yang, J. / Liu, B. / Huang, Y. / Blumenthal, R.M. / Zhang, X. / Cheng, X.
History
DepositionJun 6, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 8, 2022Provider: repository / Type: Initial release
Revision 1.1Sep 7, 2022Group: Database references / Category: citation / citation_author
Item: _citation.journal_abbrev / _citation.title / _citation_author.name
Revision 1.2Jan 25, 2023Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.name
Revision 1.3Feb 15, 2023Group: Database references / Category: citation / Item: _citation.journal_volume
Revision 1.4Oct 25, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Zinc finger and BTB domain-containing protein 7A
B: Zinc finger and BTB domain-containing protein 7A
F: Zinc finger and BTB domain-containing protein 7A
X: DNA Strand I
Y: DNA Strand II
H: DNA Strand I
I: DNA Strand II
D: DNA Strand I
E: DNA Strand II
hetero molecules


Theoretical massNumber of molelcules
Total (without water)79,82817
Polymers79,3059
Non-polymers5238
Water181
1
A: Zinc finger and BTB domain-containing protein 7A
X: DNA Strand I
Y: DNA Strand II
hetero molecules


Theoretical massNumber of molelcules
Total (without water)26,5665
Polymers26,4353
Non-polymers1312
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Zinc finger and BTB domain-containing protein 7A
D: DNA Strand I
E: DNA Strand II
hetero molecules


Theoretical massNumber of molelcules
Total (without water)26,6977
Polymers26,4353
Non-polymers2624
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
F: Zinc finger and BTB domain-containing protein 7A
H: DNA Strand I
I: DNA Strand II
hetero molecules


Theoretical massNumber of molelcules
Total (without water)26,5665
Polymers26,4353
Non-polymers1312
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)51.292, 60.067, 133.729
Angle α, β, γ (deg.)90.000, 98.502, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 379 through 401 or (resid 402...
d_2ens_1(chain "F" and ((resid 379 through 381 and (name N...
d_1ens_2chain "D"
d_2ens_2(chain "H" and (resid 1 through 14 or (resid 15...
d_3ens_2(chain "X" and (resid 1 through 14 or (resid 15...
d_1ens_3(chain "E" and (resid 1 through 15 or (resid 16...
d_2ens_3(chain "I" and (resid 1 through 15 or (resid 16...
d_3ens_3(chain "Y" and (resid 1 through 15 or (resid 16...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1LYSGLYA1 - 56
d_21ens_1LYSGLYI1 - 56
d_11ens_2DADCP
d_21ens_2DADCN
d_31ens_2DADCL
d_11ens_3DGDTQ
d_21ens_3DGDTO
d_31ens_3DGDTM

NCS ensembles :
ID
ens_1
ens_2
ens_3

NCS oper:
IDCodeMatrixVector
1given(0.130800678349, -0.677114498261, -0.724159608641), (-0.693509207932, -0.584470289353, 0.42123563403), (-0.708474530986, 0.447113449929, -0.546034249691)-15.3940848472, 59.6245027417, -81.5115377629
2given(-0.106827484986, 0.750003058391, -0.652750565573), (0.762067834659, -0.359911884166, -0.538252776132), (-0.638623914232, -0.554940400439, -0.533104725294)-2.32299743829, 32.8985942194, -24.6610116095
3given(-0.146435222703, 0.736617886803, 0.660265714992), (-0.652457845724, -0.573614348955, 0.495242706383), (0.743542524073, -0.358274570008, 0.56460946448)-18.7879822946, 32.333032574, -51.9732457752
4given(-0.0990823439393, 0.771834944826, -0.628055337582), (0.779740026541, -0.331899993572, -0.530893478277), (-0.61821370102, -0.542322055805, -0.568945171047)-2.65869982475, 32.1531015776, -25.225229292
5given(-0.156361589238, 0.723291652482, 0.672607046396), (-0.64901964737, -0.588566690593, 0.482040193398), (0.744529751412, -0.361162617355, 0.561459716353)-18.1236374466, 32.553400974, -51.7314226705

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Components

#1: Protein Zinc finger and BTB domain-containing protein 7A / Factor binding IST protein 1 / FBI-1 / Factor that binds to inducer of short transcripts protein 1 ...Factor binding IST protein 1 / FBI-1 / Factor that binds to inducer of short transcripts protein 1 / HIV-1 1st-binding protein 1 / Leukemia/lymphoma-related factor / POZ and Krueppel erythroid myeloid ontogenic factor / POK erythroid myeloid ontogenic factor / Pokemon / Pokemon 1 / TTF-I-interacting peptide 21 / TIP21 / Zinc finger protein 857A


Mass: 16638.525 Da / Num. of mol.: 3 / Fragment: zinc finger domain (UNP residues 369-500)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ZBTB7A, FBI1, LRF, ZBTB7, ZNF857A / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Gold / References: UniProt: O95365
#2: DNA chain DNA Strand I


Mass: 4876.194 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
#3: DNA chain DNA Strand II


Mass: 4920.188 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
#4: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Zn
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.57 Å3/Da / Density % sol: 52.12 %
Crystal growTemperature: 289 K / Method: vapor diffusion, sitting drop / pH: 6.5 / Details: 0.1 M Bis-Tris, pH 6.5, 20% PEG5000 MME

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 6, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 3.08→43.47 Å / Num. obs: 27875 / % possible obs: 96 % / Redundancy: 5.4 % / Biso Wilson estimate: 88.56 Å2 / Rmerge(I) obs: 0.142 / Rpim(I) all: 0.067 / Net I/σ(I): 7.8
Reflection shellResolution: 3.08→3.2 Å / Redundancy: 4.3 % / Rmerge(I) obs: 0.531 / Mean I/σ(I) obs: 1.8 / Num. unique obs: 2492 / CC1/2: 0.992 / Rpim(I) all: 0.274 / % possible all: 86.7

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Processing

Software
NameVersionClassification
SERGUIdata collection
PHENIX1.19.1_4122refinement
SERGUIdata collection
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 7N5U

7n5u


Resolution: 3.08→43.47 Å / SU ML: 0.5473 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 44.7648
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.3136 1391 5.03 %
Rwork0.2598 26251 -
obs0.2627 27642 94.29 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 141.4 Å2
Refinement stepCycle: LAST / Resolution: 3.08→43.47 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms1705 1947 8 1 3661
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00353921
X-RAY DIFFRACTIONf_angle_d0.57565682
X-RAY DIFFRACTIONf_chiral_restr0.0347620
X-RAY DIFFRACTIONf_plane_restr0.004399
X-RAY DIFFRACTIONf_dihedral_angle_d26.27321583
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS0.669167902998
ens_2d_2XX-RAY DIFFRACTIONTorsion NCS1.65934053102
ens_2d_3XX-RAY DIFFRACTIONTorsion NCS1.01144792603
ens_3d_2YX-RAY DIFFRACTIONTorsion NCS1.45274751178
ens_3d_3YX-RAY DIFFRACTIONTorsion NCS0.957290176408
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.08-3.190.47081400.41272268X-RAY DIFFRACTION80
3.19-3.310.38671210.3832605X-RAY DIFFRACTION94.72
3.31-3.470.40721470.37292660X-RAY DIFFRACTION95.57
3.47-3.650.36091410.3692648X-RAY DIFFRACTION96.14
3.65-3.880.37951340.36192745X-RAY DIFFRACTION98.16
3.88-4.180.34841460.28052735X-RAY DIFFRACTION97.66
4.18-4.60.31841420.26172555X-RAY DIFFRACTION91.7
4.6-5.260.35551400.23512654X-RAY DIFFRACTION96.28
5.26-6.620.28371420.21772754X-RAY DIFFRACTION98.27
6.63-43.470.22231380.16962627X-RAY DIFFRACTION94.89
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.91135644570.8877879186711.092025470164.335264040342.853039080812.285762390810.389304370388-0.464366890383-0.1046214488771.01968159378-0.322056462494-0.5403929659510.1089343670630.344628642014-0.03910311485921.39217441363-0.457571964575-0.7875742107581.18546776493-0.3603620643650.839987233486-15.026144747220.5958055781-31.1806665494
27.217578927583.005573787065.902700061453.041073905133.685813436325.671456082060.177225377889-0.453436844221-0.09498707460450.193785456926-0.2439429167570.3423532448780.184240546564-0.908625933856-0.2266399007430.658581465071-0.119063375498-0.8575335404791.67852892778-0.02076838695980.475147793938-19.519589926429.5884596105-37.2311018854
33.40442175016-2.719260516933.936042607952.86622915189-2.086463885236.274799188620.1015318432771.03635046123-0.7538087272810.0440775479771-0.01720836479790.0904171191821.15967801647-0.761352255533-0.2062528506951.86359402224-0.424488760276-0.5353540333621.84207434322-0.006589261280350.958976275535-13.6695468119.6259287278-35.7781047713
42.4793918865-0.191803612638-0.7507401364842.948128972351.279646560531.984637249650.03938110000130.4852230222030.0547809960653-0.3920233321670.02630039522420.0841994161275-0.00205925222057-0.09559986155370.1065962059021.29804713397-0.147908191675-0.7852097108741.46378726417-0.5944704574840.342459037848-12.16261536926.5532563242-42.5351546066
55.77743913291.72379233581-1.642255478252.405343705132.455444558765.051197236090.5813934134420.637227844663-0.638537921779-0.570359119183-0.21777646680.258241074838-0.0330612956312-0.0825020370102-0.2166842795111.93209859609-0.00041047811674-0.6510166585232.217840710410.09134688415060.711745130285-7.0193401337729.81805895-48.893800317
68.549128454380.677696806705-6.654554239172.665394423810.5874100245245.69660679251-0.58699887186-0.2921773601540.180078500642-0.461135745259-3.144749385530.709767707563-1.65648416879-4.845269019743.704628612.45576626379-0.086044619769-0.7807772913052.46517076279-0.3174318498741.680897707052.0004435436322.771980035-54.4890162264
76.610167000210.639759039128-6.182200497490.647651708366-0.03370929644786.336448190190.3835936357380.0514517923765-0.160441200607-1.619176354-0.40664860307-0.412145866389-0.6128192861811.083759099260.006011985446172.746242036490.222268060475-0.1660169167882.070857416-0.07063824345280.593319471093-3.3202181822723.5648263056-53.9317033286
87.25251613216-0.2041744412866.828351289638.4014015623-4.612952244418.75318204088-0.3627904963554.11679714518-1.82018996361-2.99248217873-0.594015420258-0.418189821545-0.9475968153250.664194441930.9815371774062.00217016751-0.0247103986706-0.08276099340462.59322329064-0.4125729172410.99580913567-2.3519319064614.4160540058-54.7701046131
98.844953347854.97378233622-2.983374203093.12858496059-1.213933164089.349101756910.6688579608940.573558911856-0.8135357782030.572880512336-0.375954634266-1.0938102982-0.03161300177171.06730241706-0.0663968870661.031031027810.293517255398-0.1446095839020.72278922530.2826396385340.41519861384515.07457024822.113619772046.47550148842
106.61645818787-1.36915918683-4.936273828233.613366725171.531358262523.76991979260.5031485655431.429933405031.59933677042-0.603322841970.9215535442721.03886768631-0.631461223136-0.413178838596-1.057396946891.10500599696-0.0760830255553-0.5633703028631.358767141480.4163709369111.378406707441.1488745832916.89767989763.02438103939
115.86442544119-6.317711573147.570542806976.82773003688-8.135455378469.79278291491-0.4516584399410.5396038839310.915539348069-1.53833715419-0.423339021577-0.0543962769022-1.27161592484-0.4097321929140.8093662456351.48615072139-0.225443972178-0.6524275878461.429080814940.1562258888311.098353568127.5620490712123.5246830454-1.69508187059
123.981035625610.5397495127652.392301094353.988616647111.149963276234.92181060125-0.3284983893760.156223379840.4017283635130.02655952590320.0998346954966-0.273794624089-0.4694552209060.3161069500440.01603771796730.99014333703-0.29052767476-0.535819866731.51397693889-0.09107609973340.660261679632-1.8091143280231.9298805997-14.723903046
135.57484830265-2.743423328682.651181806233.94587238918-1.457641929653.313092734670.0344848676176-0.04820253680620.451393544405-0.1915135007390.271203069754-0.0274935145038-0.113492579196-0.429976531198-0.1475534133210.558433033860.334491412792-0.7901187796851.80073502812-0.2860068050640.361340946561-11.680759295336.7547681793-24.9843200506
144.579040198752.691631685491.604341886392.495532073432.022820117191.83735389944-1.44170389721.120108551672.20723497037-2.101602892350.7628836170341.35857413774-1.505007966240.3700128244340.9260637384691.56426256767-0.434116035486-0.7347901969761.430476431090.08282036212081.26751439217-8.8999318992545.9084969217-44.2705951055
151.17435580692-0.739409907772-2.061716322550.8860862520751.408121787163.64017783538-0.7710243234360.8225513904640.9355938192-0.349084159981-0.1700572279060.215872328743-0.8736375892540.5339633685940.8526272866721.185779660690.174043619058-0.5594274224521.724585758030.0418671340530.897612769756-7.8079229412941.2849766448-38.5145544618
162.919726147680.983377875388-1.544690899792.22665496526-0.1210400552180.902776410213-0.0755253856730.294246713643-0.197786806278-0.111620651579-0.05595985310340.0993853578323-0.06371421214750.0264569417101-0.2076123683440.850098092206-0.109760944866-1.14224520171.680119770660.3407423621890.741951899479-4.3868882672335.0337479808-37.6866239013
172.13064342780.1445963314050.6615625761090.4862018441790.1332519227470.223605566024-0.363660284312-0.638716195811-1.42192543032-0.3440002582660.6405601458530.465542199373-0.9746767458370.947193882553-0.283831084411.418373507340.396243241968-0.5302450942151.58576475930.02123494199940.9013731175524.559902004623.8811830596-40.1927984712
182.00424850134-1.659089369351.674952844374.435059739-2.424625129488.71164112679-0.090820083870.339453170719-0.248532997359-0.163956905282-0.554054827366-0.9815280150961.42278346122.22219741231-0.1731657987841.72841957090.539597352771-0.6909670511282.25761969450.1605542857820.8419633350037.2838689641225.4613238743-39.1146630323
199.11068652727-2.1954411236.409613388941.48995169384-1.811524791164.597068255240.1194602501531.459907442860.17117812893-0.60743526306-0.349920132206-1.062380274960.3852028356633.821947579610.08593948969131.49313698950.385770212557-0.1829853264413.15738897368-0.01549656135320.8886560372914.319605588129.7130527114-43.5924422347
204.705653676640.9175092887741.026256461394.423951578275.013801812935.68286897590.1351297869281.01822543325-0.548108691279-0.5264030957230.585079738197-0.3801394307360.7715960088530.209064757571-0.8310348122232.39931256027-0.306859090317-0.6409894672252.1643841159-0.3986338890171.17517690525-18.196383001211.957315284-52.5050388531
219.70770421497-0.178322285957-0.3426919698147.418425501227.494591664118.135093381850.1264298929531.07599897546-0.8504753492130.20791323807-0.8555861477830.1658468736530.3603441279781.228649066070.5889561357512.47940189550.360715368855-0.4125791469222.133408143570.02353425657830.770872129716-3.7138102585113.6775503224-40.5162398847
227.231279778952.20023224497.628137585140.7565399986082.057489407978.75929137463-0.371725267249-0.7210353132050.1041758315231.582859493740.669908849043-1.03035362312-1.01765131220.51521104125-0.1440536172182.54185501228-0.072661819606-0.6853113638892.26282812445-0.2647151564681.32742833932-21.605544662112.5623225006-60.1098211386
238.470406728042.30056435154.871937746037.171518868033.819225587928.796980448610.4454427779311.0751479137-0.397432829777-0.2217431805980.608493448536-0.429809373884-0.6493306997270.724945190464-1.165075805081.02777215045-0.0694992114681-0.3702804506251.89317197266-0.04468081065811.1357723925412.665086754843.7447429067-33.1662748759
242.22626894087-3.698779275540.2150656887426.497533004891.646136895582.00005447023-0.4743990509131.10739376020.204913252454-0.396832506252-0.802605196645-1.579296097980.2555831072515.443324530981.46767080291.33090729670.75028445507-0.203870703862.944475909010.5662076248291.006036535125.6151178491441.3792259703-58.0371448374
255.036118934591.718364873254.879698271245.427029309141.402451397484.761526959390.4996041954392.021760958540.426456553814-0.8935976768320.0818957165896-0.06870345660050.769547285770.805508579442-0.389497058531.848922855090.129187476744-0.401839635152.480094928430.2947397416130.9613314014514.3520488898837.1469836552-50.3939618336
267.714974607671.253384092716.005379067567.099806628680.2618393628.493040141310.5095038346270.144309210271-1.12118167996-0.2483751959220.4720362942430.674463806602-0.390027203029-0.553188364938-1.163324415660.766041356862-0.17524483201-0.07824802692121.91693459017-0.2020688003770.92739457422316.722436341742.5090347755-26.6588888279
274.62491596185.311508569534.636322976386.082871452085.314199757474.64339792602-1.367941662882.12777066449-0.48470346173-2.832845494881.244699521781.219268823181.292580836030.4693664324440.05500069380012.16327474644-0.147939929803-0.3093758264071.5545817305-0.0856160376851.356586337095.7561089615811.250722958-26.8694947162
289.00965744859-0.9718823149836.161770324524.83465175458-2.943108288635.39133551048-0.6155593973291.268465816260.668743449664-1.301058713390.171940198073-0.119365061302-1.258623238030.418614931230.421182411571.559172187860.105343359644-0.1611111174251.576650520240.09509475936360.77531725544216.105575484913.1921077156-2.52732127211
291.421395576682.890935927641.622034990238.730761005767.531491186078.12269423947-1.00326473460.7778395822760.110997771428-2.45870086353-0.1468126278920.235979891946-2.062945892530.7737300221091.019408775161.6220637291-0.0149785345745-0.3660889328581.5055806230.1960833193010.90492174341416.821428821113.8052432088-2.13417742167
307.60957461689-6.478199458055.977680943495.52112735232-5.09042200094.69164690589-1.518622063170.288466856652-0.1557110513652.788362153591.35252806570.637429522043-3.34160081915-1.318610690180.02043383740742.251924581550.35484724001-0.6235723448371.807466659910.1227568625561.4156352910.71593890615114.1032574436-21.2389407882
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 379 through 383 )AA379 - 3831 - 5
22chain 'A' and (resid 384 through 389 )AA384 - 3896 - 11
33chain 'A' and (resid 390 through 396 )AA390 - 39612 - 18
44chain 'A' and (resid 397 through 404 )AA397 - 40419 - 26
55chain 'A' and (resid 405 through 409 )AA405 - 40927 - 31
66chain 'A' and (resid 410 through 414 )AA410 - 41432 - 36
77chain 'A' and (resid 415 through 421 )AA415 - 42137 - 43
88chain 'A' and (resid 422 through 434 )AA422 - 43444 - 56
99chain 'B' and (resid 379 through 404 )BD379 - 4041 - 26
1010chain 'B' and (resid 405 through 421 )BD405 - 42127 - 43
1111chain 'B' and (resid 422 through 433 )BD422 - 43344 - 55
1212chain 'B' and (resid 434 through 450 )BD434 - 45056 - 72
1313chain 'B' and (resid 451 through 491 )BD451 - 49173 - 113
1414chain 'F' and (resid 379 through 383 )FI379 - 3831 - 5
1515chain 'F' and (resid 384 through 396 )FI384 - 3966 - 18
1616chain 'F' and (resid 397 through 404 )FI397 - 40419 - 26
1717chain 'F' and (resid 405 through 414 )FI405 - 41427 - 36
1818chain 'F' and (resid 415 through 421 )FI415 - 42137 - 43
1919chain 'F' and (resid 422 through 434 )FI422 - 43444 - 56
2020chain 'X' and (resid 2 through 15 )XL2 - 15
2121chain 'Y' and (resid 1 through 5 )YM1 - 5
2222chain 'Y' and (resid 6 through 16 )YM6 - 16
2323chain 'H' and (resid 1 through 15 )HN1 - 15
2424chain 'H' and (resid 16 through 16 )HN16
2525chain 'I' and (resid 1 through 5 )IO1 - 5
2626chain 'I' and (resid 6 through 16 )IO6 - 16
2727chain 'D' and (resid 1 through 5 )DP1 - 5
2828chain 'D' and (resid 6 through 16 )DP6 - 16
2929chain 'E' and (resid 1 through 10 )EQ1 - 10
3030chain 'E' and (resid 11 through 16 )EQ11 - 16

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