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- PDB-8e3e: ZBTB7A Zinc Finger Domain Bound to DNA Duplex Containing CAST seq... -

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Basic information

Entry
Database: PDB / ID: 8e3e
TitleZBTB7A Zinc Finger Domain Bound to DNA Duplex Containing CAST sequence (#10)
Components
  • DNA (5'-D(*CP*TP*TP*TP*GP*GP*GP*GP*AP*GP*GP*GP*GP*TP*CP*TP*TP*TP*TP*AP*C)-3')
  • DNA (5'-D(*GP*GP*TP*AP*AP*AP*AP*GP*AP*CP*CP*CP*CP*TP*CP*CP*CP*CP*AP*AP*A)-3')
  • Zinc finger and BTB domain-containing protein 7A
KeywordsDNA BINDING PROTEIN/DNA / gene expression / DNA BINDING PROTEIN-DNA complex / DNA BINDING PROTEIN
Function / homology
Function and homology information


regulation of transcription regulatory region DNA binding / DNA-dependent protein kinase complex / negative regulation of androgen receptor signaling pathway / double-strand break repair via classical nonhomologous end joining / regulation of DNA-binding transcription factor activity / regulation of glycolytic process / nuclear androgen receptor binding / erythrocyte maturation / regulation of alternative mRNA splicing, via spliceosome / histone acetyltransferase binding ...regulation of transcription regulatory region DNA binding / DNA-dependent protein kinase complex / negative regulation of androgen receptor signaling pathway / double-strand break repair via classical nonhomologous end joining / regulation of DNA-binding transcription factor activity / regulation of glycolytic process / nuclear androgen receptor binding / erythrocyte maturation / regulation of alternative mRNA splicing, via spliceosome / histone acetyltransferase binding / SMAD binding / fat cell differentiation / negative regulation of Notch signaling pathway / protein localization to nucleus / B cell differentiation / transcription corepressor binding / negative regulation of transforming growth factor beta receptor signaling pathway / positive regulation of NF-kappaB transcription factor activity / DNA-binding transcription repressor activity, RNA polymerase II-specific / sequence-specific double-stranded DNA binding / site of double-strand break / chromatin organization / regulation of apoptotic process / DNA-binding transcription factor binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / chromatin remodeling / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / DNA-templated transcription / regulation of transcription by RNA polymerase II / negative regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleus / cytoplasm
Similarity search - Function
: / BTB/POZ domain / BTB domain profile. / Zinc finger, C2H2 type / Broad-Complex, Tramtrack and Bric a brac / BTB/POZ domain / zinc finger / Zinc finger C2H2 type domain profile. / Zinc finger C2H2 superfamily / Zinc finger C2H2 type domain signature. ...: / BTB/POZ domain / BTB domain profile. / Zinc finger, C2H2 type / Broad-Complex, Tramtrack and Bric a brac / BTB/POZ domain / zinc finger / Zinc finger C2H2 type domain profile. / Zinc finger C2H2 superfamily / Zinc finger C2H2 type domain signature. / SKP1/BTB/POZ domain superfamily / Zinc finger C2H2-type
Similarity search - Domain/homology
DNA / DNA (> 10) / Zinc finger and BTB domain-containing protein 7A
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.99 Å
AuthorsHorton, J.R. / Ren, R. / Cheng, X.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM134744 United States
Cancer Prevention and Research Institute of Texas (CPRIT)RR160029 United States
CitationJournal: J.Biol.Chem. / Year: 2023
Title: Structural basis for transcription factor ZBTB7A recognition of DNA and effects of ZBTB7A somatic mutations that occur in human acute myeloid leukemia.
Authors: Ren, R. / Horton, J.R. / Chen, Q. / Yang, J. / Liu, B. / Huang, Y. / Blumenthal, R.M. / Zhang, X. / Cheng, X.
History
DepositionAug 17, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 8, 2023Provider: repository / Type: Initial release
Revision 1.1Feb 15, 2023Group: Database references / Category: citation / Item: _citation.journal_volume
Revision 1.2Oct 25, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.3Oct 9, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Zinc finger and BTB domain-containing protein 7A
X: DNA (5'-D(*CP*TP*TP*TP*GP*GP*GP*GP*AP*GP*GP*GP*GP*TP*CP*TP*TP*TP*TP*AP*C)-3')
Y: DNA (5'-D(*GP*GP*TP*AP*AP*AP*AP*GP*AP*CP*CP*CP*CP*TP*CP*CP*CP*CP*AP*AP*A)-3')
B: Zinc finger and BTB domain-containing protein 7A
D: DNA (5'-D(*CP*TP*TP*TP*GP*GP*GP*GP*AP*GP*GP*GP*GP*TP*CP*TP*TP*TP*TP*AP*C)-3')
E: DNA (5'-D(*GP*GP*TP*AP*AP*AP*AP*GP*AP*CP*CP*CP*CP*TP*CP*CP*CP*CP*AP*AP*A)-3')
F: Zinc finger and BTB domain-containing protein 7A
H: DNA (5'-D(*CP*TP*TP*TP*GP*GP*GP*GP*AP*GP*GP*GP*GP*TP*CP*TP*TP*TP*TP*AP*C)-3')
I: DNA (5'-D(*GP*GP*TP*AP*AP*AP*AP*GP*AP*CP*CP*CP*CP*TP*CP*CP*CP*CP*AP*AP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)97,00921
Polymers96,2249
Non-polymers78512
Water1629
1
A: Zinc finger and BTB domain-containing protein 7A
X: DNA (5'-D(*CP*TP*TP*TP*GP*GP*GP*GP*AP*GP*GP*GP*GP*TP*CP*TP*TP*TP*TP*AP*C)-3')
Y: DNA (5'-D(*GP*GP*TP*AP*AP*AP*AP*GP*AP*CP*CP*CP*CP*TP*CP*CP*CP*CP*AP*AP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)32,3367
Polymers32,0753
Non-polymers2624
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4990 Å2
ΔGint-43 kcal/mol
Surface area13020 Å2
MethodPISA
2
B: Zinc finger and BTB domain-containing protein 7A
D: DNA (5'-D(*CP*TP*TP*TP*GP*GP*GP*GP*AP*GP*GP*GP*GP*TP*CP*TP*TP*TP*TP*AP*C)-3')
E: DNA (5'-D(*GP*GP*TP*AP*AP*AP*AP*GP*AP*CP*CP*CP*CP*TP*CP*CP*CP*CP*AP*AP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)32,3367
Polymers32,0753
Non-polymers2624
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4940 Å2
ΔGint-42 kcal/mol
Surface area13070 Å2
MethodPISA
3
F: Zinc finger and BTB domain-containing protein 7A
H: DNA (5'-D(*CP*TP*TP*TP*GP*GP*GP*GP*AP*GP*GP*GP*GP*TP*CP*TP*TP*TP*TP*AP*C)-3')
I: DNA (5'-D(*GP*GP*TP*AP*AP*AP*AP*GP*AP*CP*CP*CP*CP*TP*CP*CP*CP*CP*AP*AP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)32,3367
Polymers32,0753
Non-polymers2624
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5110 Å2
ΔGint-38 kcal/mol
Surface area13830 Å2
MethodPISA
Unit cell
Length a, b, c (Å)67.519, 147.175, 68.064
Angle α, β, γ (deg.)90.000, 96.460, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

#1: Protein Zinc finger and BTB domain-containing protein 7A / Factor binding IST protein 1 / FBI-1 / Factor that binds to inducer of short transcripts protein 1 ...Factor binding IST protein 1 / FBI-1 / Factor that binds to inducer of short transcripts protein 1 / HIV-1 1st-binding protein 1 / Leukemia/lymphoma-related factor / POZ and Krueppel erythroid myeloid ontogenic factor / POK erythroid myeloid ontogenic factor / Pokemon / Pokemon 1 / TTF-I-interacting peptide 21 / TIP21 / Zinc finger protein 857A


Mass: 19188.326 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ZBTB7A, FBI1, LRF, ZBTB7, ZNF857A / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): Gold / References: UniProt: O95365
#2: DNA chain DNA (5'-D(*CP*TP*TP*TP*GP*GP*GP*GP*AP*GP*GP*GP*GP*TP*CP*TP*TP*TP*TP*AP*C)-3')


Mass: 6516.189 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
#3: DNA chain DNA (5'-D(*GP*GP*TP*AP*AP*AP*AP*GP*AP*CP*CP*CP*CP*TP*CP*CP*CP*CP*AP*AP*A)-3')


Mass: 6370.154 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
#4: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: Zn
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 9 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.49 Å3/Da / Density % sol: 64.78 %
Crystal growTemperature: 292 K / Method: vapor diffusion, sitting drop / pH: 7 / Details: 12% PEG3350, 0.1M Sodium malonate pH7.0

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jun 9, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.99→49.8 Å / Num. obs: 26193 / % possible obs: 98.7 % / Redundancy: 7.1 % / Biso Wilson estimate: 58.8 Å2 / Rmerge(I) obs: 0.08 / Rpim(I) all: 0.044 / Net I/σ(I): 15.7
Reflection shellResolution: 2.99→3.1 Å / Redundancy: 6.4 % / Rmerge(I) obs: 0.42 / Mean I/σ(I) obs: 2.1 / Num. unique obs: 2577 / CC1/2: 0.945 / Rpim(I) all: 0.331 / % possible all: 97.6

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Processing

Software
NameVersionClassification
SERGUIdata collection
PHENIX1.20.1_4487refinement
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7N5U

7n5u


Resolution: 2.99→49.8 Å / SU ML: 0.3106 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 31.0861
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2354 1284 4.99 %
Rwork0.2031 24441 -
obs0.2047 25725 96.69 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 119.28 Å2
Refinement stepCycle: LAST / Resolution: 2.99→49.8 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2703 2565 12 9 5289
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00315642
X-RAY DIFFRACTIONf_angle_d0.51438138
X-RAY DIFFRACTIONf_chiral_restr0.0317883
X-RAY DIFFRACTIONf_plane_restr0.0036609
X-RAY DIFFRACTIONf_dihedral_angle_d24.91872285
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.99-3.110.36571360.32512597X-RAY DIFFRACTION92.77
3.11-3.260.32251390.31172591X-RAY DIFFRACTION92.95
3.26-3.430.30151420.2612692X-RAY DIFFRACTION96.26
3.43-3.640.29921360.24162674X-RAY DIFFRACTION95.71
3.64-3.920.28331430.23712729X-RAY DIFFRACTION96.86
3.92-4.320.2431460.212772X-RAY DIFFRACTION99.29
4.32-4.940.23431470.19482799X-RAY DIFFRACTION99.39
4.94-6.220.21871490.19442814X-RAY DIFFRACTION99.63
6.23-49.80.18181460.1562773X-RAY DIFFRACTION97.33
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.81890834306-0.1875940860973.569380265159.181941446652.396462830075.34480261207-0.577214660757-0.36295087397-0.0798560899675-0.704831874629-0.122150229050.50958877478-0.2426532017720.3857935965280.5603144644650.7021504124060.2130764507550.05533587407710.6668437717260.08674011169670.418973199693-23.274722799520.883679182-34.2003216689
21.584000944330.970789792332.137765106182.57333682216-0.639828027864.626824377481.108388812160.1855318698830.09152492346560.260654588519-0.624330023215-0.1205493626710.202076355633-0.0118959385431-0.4210895165390.457257299123-0.0370592038850.1091319715011.01659307581-0.0618484858190.368643359928-21.2386966913-1.47976077423-23.1884874132
36.06251409399-3.710268013810.1332143245375.5340412607-2.829680252262.43366306965-0.199920033079-1.297357244550.8871521890071.628944477750.318685048352-0.477436724321-1.24956710742-0.120076059931-0.1282424209361.138249511260.08145953739530.1244301262441.46350127960.0363328766228-0.352540820598-9.2235406934812.9813839023-7.48494041196
42.03396159885-0.934604308544-3.92511510510.4069875021731.791814727047.54927666048-0.287883254488-0.8237622450970.601648082589-0.4315566562451.09612196308-0.0165416395212-2.32248593958-0.229287063562-0.201409868621.667313286050.245657794240.3763615658381.681862585060.1473487717070.419277613226-19.770029154512.12644150390.33221347961
52.45430021346-1.549252607710.8615392626893.26381750208-1.675223343242.72293152050.3337228074470.4343533068580.217683371377-0.3992611446070.3241825788460.1083606557110.513723906185-1.50282138714-0.6336177187070.978870020807-0.08541120536890.06353150855871.401300720830.1987966814690.563169555173-18.62550533959.90809283695-32.6835807995
66.16015805779-1.81558950167-3.546745888591.379496513932.250995423617.342081399711.09230686139-0.05878598588240.39531520463-0.8757914603770.493043717209-0.387528739363-0.9176425128741.20569499743-0.9870931295821.872861654060.4165787471280.2916876079372.059569193040.0507638009980.560109233398-11.685517510113.0229578406-54.0823877626
71.03576416143-1.4387304506-0.5297760460552.213384755691.824490693095.150131882110.524356542621-0.06527324495690.5306809998430.2420571698680.5370433055510.0130177921549-0.586163511813-0.496239675065-0.4566600936191.122288195960.1670739568280.3299834670441.354110054520.2492248089420.543572676636-17.06496739399.86642725548-29.8474304792
81.787776726290.0493617575126-0.6957137348891.85692677279-3.246746970437.534511178680.0993389836959-0.573314432386-0.2221403334670.549596524757-0.07994512346460.615207567516-0.325701637694-1.07729836583-0.1589148488461.53022548480.2490996803630.529378502052.160992112310.4700026964750.421577722421-27.403780635610.4459856217-6.19648202733
92.23590101831-3.9030686404-1.428682283758.008105863312.46323635790.921863856899-0.1804219341220.209917393417-0.2531181432930.6649158066-0.597517435088-0.316339672851.104146050850.5462420858330.5789614347681.89732250659-0.103128121434-0.01041398946852.599216194340.2533041337350.80799575992-21.47647947284.422222277852.79685511949
105.919145745125.906477956872.160675070556.021681084662.234824360381.970406763071.57903689242-0.151858254175-2.746718636151.23661782481-0.617145585512-1.249423599322.73000250267-0.220129017448-1.012438696311.0605559076-0.019577016762-0.1339256892520.888445401428-0.07722339585651.34321874602-30.4797415224-51.7718505138-21.2097747152
118.315674419592.816558309953.41498658037.38941484734-0.4337083134929.090889562450.8833342175070.499030346496-1.056666736860.796060699380.298922002099-0.2302804300640.649593095115-0.0231006709254-0.9471917809970.724018632336-0.3273867234060.01192860465960.874354255591-0.2700980525590.887768719579-31.0675463649-43.2318112298-23.9837443766
125.335533864843.73428935934-4.231189984483.96342810105-4.735532245785.684501324350.2266571273321.11927406914-0.361974132611-1.04181841829-0.0633751209753-0.475342552798-0.0881318735901-0.8786406803460.7037756337841.00639963743-0.4450535596560.6202574321721.64905908112-0.06485065706460.643648171803-24.2470931429-31.12733772-37.0428705849
133.639279460920.7877734327714.296377365636.49095394792-0.1568309449055.25978339136-1.429666879940.0953899317170.0710670407158-1.007513406830.421813239249-0.895204254355-0.4469444404050.05236154415060.6455231938810.93828188503-0.3525419630190.1791644826821.25987125618-0.1389856086130.533508671877-23.5542121732-26.8589477084-31.0182361472
147.872452665594.395404182995.888415432438.17688275074.841030160167.41332057549-0.173093579711-0.1935557805610.105162918428-0.493009744482-0.3303722191130.914035260777-0.130857687123-0.06644927695470.522807506780.606558319221-0.241624994032-0.02161694535020.678894754056-0.005869251926480.453629767815-18.4890324496-15.3994955751-13.9293324082
157.772478332-1.73858717539-3.125299210667.222169295853.229583001333.219668041670.808829299657-2.10953331722-0.2761463867862.528502707740.888793984158-1.807536829871.391286414331.94485868923-1.391329725941.44306373898-0.21374097507-0.3818129173031.50530258084-0.1093508665471.0674887725-9.03806757008-25.3216738489-3.92628675874
162.38227226703-3.068089288923.90127876574.11269975749-4.77997968086.761871776630.253712825152-0.382042716223-0.1197589761231.53806904564-0.632843965113-1.07806464825-0.9413830274940.7187758076260.4368818807230.79755496327-0.765488630875-0.2385985598721.795843663090.4365610571741.19335310185-7.40738073685-26.690259179-10.4075214623
173.808650543721.15672248067-0.07637425693369.532479945836.742552087974.989086907910.418592535458-1.15302562719-1.655011541141.465611237160.453078792975-3.031527403721.039808924822.40363319511-0.715988041470.6636482153140.0495446344782-0.1446829999671.927631302930.2419331319581.83749988858-7.80211016997-35.3837112024-9.59706116013
189.39217853633-5.951929483642.877045706467.84773401193-3.708235948067.2798617513-0.39408111332-0.163100063478-1.428312957470.4511776095230.263315248037-1.18471165697-0.009550131556271.20146877780.278750304590.6814836812790.06247010834130.06768465386181.81513432722-0.3265231799411.998305582760.183763347083-33.6361120189-17.7857368392
198.335950560880.2688552959560.9093698145529.10917314804-0.6069934741045.63840385374-1.23150522355-0.6753324435911.25264268881-0.6400725151060.721406573324-0.457808299826-0.0242475939199-0.1067541719540.5191174830940.69101257959-0.1208422273340.1106152222281.309834811-0.1801615560690.971186291796-16.2518440305-33.1513170595-25.1049897353
203.31383063037-4.59620486683-3.885111013328.499030896122.108699286559.563904862990.0562505235128-0.1115137160670.7738862864251.385636057810.6619573868770.790025641570.29000264556-0.128788119705-0.6523308925930.89072543639-0.2320319989650.207422264971.37166503398-0.2551062782180.980813729269-31.3419650103-34.7188056202-15.0952742978
217.379295918054.253177885950.1541478000816.48614967948-1.590936753596.87163106059-1.297378346091.13819918574-0.0169966339842-0.2940011248280.6166611107722.393122659510.495856476331-1.628376131740.3942408715390.8164134615650.04622456958830.2846018229812.41343888851-0.3894074083241.91826669456-48.3438632184-33.3676077792-23.4813957134
223.753590133981.489015809291.50041345615.022977676580.9889975020550.617704060091-2.00897721711-0.178036855548-1.56648044568-0.3191235904210.8669719466231.752234029720.502791382108-1.372393418760.4394754992930.734168134994-0.1924594269940.340246368812.21873865044-0.3466149755921.71363537521-45.697301135-34.1241684939-19.9665764026
238.96229366314-0.9856560901380.1483427339868.567908120840.7690944077644.795867900490.1403280178681.44729766831-0.937832863303-0.780771496836-0.431693527241-1.18378554204-0.03212411182580.9795567052040.28826351990.664004134163-0.1508084007820.08457472814731.39889764576-0.1540268489241.15243610248-11.5264469725-33.4554146737-21.2055612925
243.70768755617-0.4769567546620.3246810298344.857987244090.500965931425.73557742339-0.0478223917113-0.6282466884860.385814554993-0.0446883645523-0.0595654386449-0.749899145142-0.4164938738360.7751807515870.07208528959330.751639823548-0.15999555597-0.02093890708841.32320784644-0.02734270183750.355439665713.591417747351.24262511915-1.13150677958
254.20249607968-3.037148214715.665296591682.65367494674-3.785411901998.614363144671.37066034118-0.947619300863-2.474822746070.727188437829-1.23502653604-0.5662496760221.65390215914-0.00390680257803-0.2301984096832.162541202550.0418045666405-0.5038814702631.974627992570.3388630166971.401836809663.44023916438-23.33127678366.81570288001
269.127161164833.442426157786.586494576692.066478399843.29653898977.901287181861.12093809098-1.30908126662-1.37248174161-0.334976202993-0.004360591701490.1657143828551.690014302390.434063359742-1.174639213831.45251940140.0606604516414-0.380986807111.756634357220.276198275441.0841478868614.8574940274-17.622715449216.9813631143
273.30964982044-5.304142515212.32316016448.52153428439-4.015259144655.31978339715-0.2420252164070.1640497674630.9333529394421.53625275898-0.243063168294-1.004420364270.4421001702270.9708921720860.4000363525220.99861366495-0.251547938146-0.06176330748812.419192413620.02350934063020.43938934889218.93854139-7.4224291040225.6166267321
280.889303077282-0.49416290527-0.6671639997743.81081306917-2.475937294458.026426445030.746125729788-0.05282030060660.1569980687420.1262130222210.4754730898460.418563618538-0.0437993840834-0.385388045762-0.9583512799081.15750441346-0.2787397751080.06382392637332.648706528490.1803418229730.7358845740057.03456809132-6.4906200450122.1420847016
294.53902642254-2.243802136120.1136300911452.951267085523.339100211446.485248007460.1369871991250.7854328796150.662719594944-1.13054507290.803790925984-0.4143851707280.7608680804040.650199653963-0.5219499278541.39469057085-0.3127628192770.1899641849252.451417291640.05227872585460.60867945368412.7793852371-5.15713537453-9.46111629258
301.71660496398-1.680472487490.1626700054993.132613653992.443715759334.602891292970.6371458777140.1076095261310.260503483764-0.7928904989080.866468786351-0.6768104148920.2408605607081.376305465450.4194705252791.64771177766-0.3298706226920.2242148709663.18492402676-0.2267076645050.63822062711918.2777106261-5.33488786676-22.7933143657
313.64490699579-0.1582799596230.4864158631973.197430203112.355295275447.21844130650.530146424907-0.04044638675260.30371069861-0.3124930225910.554723863913-0.2453715506670.4672306354091.25700463571-1.014025163481.14074340192-0.05049648472880.07928031918752.20504713490.0405047288110.61845002302510.9007677988-6.237943235130.401628032624
327.04217059788-1.104680501451.340304243462.299913245431.408868472423.910937579451.60258262108-1.047341423930.3341168925271.312882161760.6744231901031.01135222114-0.267193816501-0.584577721246-1.634344454141.75376393921-0.1807139527910.2444030076272.876358246560.2678348322350.8398219960852.5405837974-4.501606305425.268889704
336.59628616733-4.485458117040.3697413971878.40939676881-5.367287536864.90390332939-0.309505307918-1.01188746584-0.7122398293890.4150751760231.736003981190.597069079971-0.661187497355-2.54133196626-1.168686471822.32849566187-0.754189193446-0.1498735079482.436048621030.1527638014310.9198246518835.78798992447-12.574224776734.0161124757
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 381 through 411 )AA381 - 4111 - 31
22chain 'A' and (resid 412 through 461 )AA412 - 46132 - 81
33chain 'A' and (resid 462 through 491 )AA462 - 49182 - 111
44chain 'X' and (resid 1 through 5 )XF1 - 5
55chain 'X' and (resid 6 through 21 )XF6 - 21
66chain 'Y' and (resid 1 through 5 )YG1 - 5
77chain 'Y' and (resid 6 through 15 )YG6 - 15
88chain 'Y' and (resid 16 through 20 )YG16 - 20
99chain 'Y' and (resid 21 through 21 )YG21
1010chain 'B' and (resid 381 through 390 )BH381 - 3901 - 10
1111chain 'B' and (resid 391 through 405 )BH391 - 40511 - 25
1212chain 'B' and (resid 406 through 415 )BH406 - 41526 - 35
1313chain 'B' and (resid 416 through 433 )BH416 - 43336 - 53
1414chain 'B' and (resid 434 through 461 )BH434 - 46154 - 81
1515chain 'B' and (resid 462 through 471 )BH462 - 47182 - 91
1616chain 'B' and (resid 472 through 477 )BH472 - 47792 - 97
1717chain 'B' and (resid 478 through 491 )BH478 - 49198 - 111
1818chain 'D' and (resid 1 through 5 )DM1 - 5
1919chain 'D' and (resid 6 through 10 )DM6 - 10
2020chain 'D' and (resid 11 through 15 )DM11 - 15
2121chain 'D' and (resid 16 through 21 )DM16 - 21
2222chain 'E' and (resid 1 through 10 )EN1 - 10
2323chain 'E' and (resid 11 through 21 )EN11 - 21
2424chain 'F' and (resid 381 through 433 )FO381 - 4331 - 53
2525chain 'F' and (resid 434 through 449 )FO434 - 44954 - 69
2626chain 'F' and (resid 450 through 471 )FO450 - 47170 - 91
2727chain 'F' and (resid 472 through 501 )FO472 - 50192 - 121
2828chain 'H' and (resid 1 through 10 )HT1 - 10
2929chain 'H' and (resid 11 through 21 )HT11 - 21
3030chain 'I' and (resid 1 through 5 )IU1 - 5
3131chain 'I' and (resid 6 through 15 )IU6 - 15
3232chain 'I' and (resid 16 through 20 )IU16 - 20
3333chain 'I' and (resid 21 through 21 )IU21

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