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Yorodumi- PDB-7mwx: Structure of the core ectodomain of the hepatitis C virus envelop... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7mwx | ||||||
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Title | Structure of the core ectodomain of the hepatitis C virus envelope glycoprotein 2 with tamarin CD81 | ||||||
Components |
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Keywords | VIRAL PROTEIN/IMMUNE SYSTEM / Glycoprotein / Receptor / Viral protein / VIRAL PROTEIN-IMMUNE SYSTEM complex | ||||||
Function / homology | Function and homology information : / CD4-positive, alpha-beta T cell costimulation / osteoclast fusion / positive regulation of B cell receptor signaling pathway / myoblast fusion involved in skeletal muscle regeneration / positive regulation of inflammatory response to antigenic stimulus / positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell / regulation of macrophage migration / macrophage fusion / immunological synapse formation ...: / CD4-positive, alpha-beta T cell costimulation / osteoclast fusion / positive regulation of B cell receptor signaling pathway / myoblast fusion involved in skeletal muscle regeneration / positive regulation of inflammatory response to antigenic stimulus / positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell / regulation of macrophage migration / macrophage fusion / immunological synapse formation / positive regulation of T-helper 2 cell cytokine production / tetraspanin-enriched microdomain / positive regulation of B cell activation / positive regulation of protein exit from endoplasmic reticulum / humoral immune response mediated by circulating immunoglobulin / positive regulation of CD4-positive, alpha-beta T cell proliferation / host cell lipid droplet / symbiont-mediated suppression of host TRAF-mediated signal transduction / cholesterol binding / positive regulation of T cell receptor signaling pathway / transformation of host cell by virus / symbiont-mediated perturbation of host cell cycle G1/S transition checkpoint / host cell mitochondrion / cellular response to low-density lipoprotein particle stimulus / immunological synapse / positive regulation of receptor clustering / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / ribonucleoside triphosphate phosphatase activity / lipid droplet / protein localization to plasma membrane / protein localization / receptor internalization / positive regulation of peptidyl-tyrosine phosphorylation / integrin binding / protein complex oligomerization / monoatomic ion channel activity / basolateral plasma membrane / viral nucleocapsid / positive regulation of MAPK cascade / RNA helicase activity / host cell perinuclear region of cytoplasm / host cell endoplasmic reticulum membrane / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / ribonucleoprotein complex / symbiont entry into host cell / induction by virus of host autophagy / viral RNA genome replication / cysteine-type endopeptidase activity / serine-type endopeptidase activity / RNA-dependent RNA polymerase activity / virus-mediated perturbation of host defense response / fusion of virus membrane with host endosome membrane / viral envelope / positive regulation of cell population proliferation / virion attachment to host cell / host cell plasma membrane / structural molecule activity / virion membrane / proteolysis / RNA binding / zinc ion binding / ATP binding / membrane / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Hepacivirus C Mus musculus (house mouse) Saguinus oedipus (cotton-top tamarin) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.32 Å | ||||||
Authors | Kumar, A. / Hossain, R.A. / Yost, S.A. / Bu, W. / Wang, Y. / Dearborn, A.D. / Grakoui, A. / Cohen, J.I. / Marcotrigiano, J. | ||||||
Funding support | United States, 1items
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Citation | Journal: Nature / Year: 2021 Title: Structural insights into hepatitis C virus receptor binding and entry. Authors: Kumar, A. / Hossain, R.A. / Yost, S.A. / Bu, W. / Wang, Y. / Dearborn, A.D. / Grakoui, A. / Cohen, J.I. / Marcotrigiano, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7mwx.cif.gz | 551.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7mwx.ent.gz | 454.1 KB | Display | PDB format |
PDBx/mmJSON format | 7mwx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7mwx_validation.pdf.gz | 993.9 KB | Display | wwPDB validaton report |
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Full document | 7mwx_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | 7mwx_validation.xml.gz | 50.1 KB | Display | |
Data in CIF | 7mwx_validation.cif.gz | 67.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mw/7mwx ftp://data.pdbj.org/pub/pdb/validation_reports/mw/7mwx | HTTPS FTP |
-Related structure data
Related structure data | 7mwsC 7mwwC 4webS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
-Protein , 2 types, 4 molecules CGDH
#1: Protein | Mass: 29314.105 Da / Num. of mol.: 2 / Fragment: DeltaHVR1 Source method: isolated from a genetically manipulated source Details: J6 genotype / Source: (gene. exp.) Hepacivirus C / Cell line (production host): HEK293 gnti- cells / Production host: Homo sapiens (human) References: UniProt: A0A2I6PIY1, RNA-directed RNA polymerase, hepacivirin, nucleoside-triphosphate phosphatase, RNA helicase #4: Protein | Mass: 10141.375 Da / Num. of mol.: 2 / Fragment: second extracellular domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saguinus oedipus (cotton-top tamarin) / Gene: CD81 / Cell line (production host): HEK293 GNTI- cells / Production host: Homo sapiens (human) / References: UniProt: Q9N0J9 |
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-Antibody , 2 types, 4 molecules AEBF
#2: Antibody | Mass: 23207.604 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Production host: Homo sapiens (human) #3: Antibody | Mass: 24111.613 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Production host: Homo sapiens (human) |
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-Sugars , 3 types, 5 molecules
#5: Polysaccharide | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
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#6: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
#7: Sugar |
-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 56.1 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 4.5 Details: 0.2M sodium acetate pH 4.5, 4% v/v tacsimate pH 4.0, 12% v/v PEG Smear Medium |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 0.979 Å |
Detector | Type: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: Dec 7, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 3.32→54.74 Å / Num. obs: 31609 / % possible obs: 99.5 % / Redundancy: 13.2 % / CC1/2: 0.99 / Net I/σ(I): 6.8 |
Reflection shell | Resolution: 3.32→3.5 Å / Num. unique obs: 4559 / CC1/2: 0.39 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4WEB Resolution: 3.32→53.093 Å / SU ML: 0.5 / Cross valid method: FREE R-VALUE / σ(F): 1.89 / Phase error: 33.83 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.32→53.093 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -13.9078 Å / Origin y: 16.0699 Å / Origin z: -28.4282 Å
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Refinement TLS group | Selection details: all |