- PDB-7lin: X-ray structure of SPOP MATH domain (D140G) in complex with a 53B... -
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基本情報
登録情報
データベース: PDB / ID: 7lin
タイトル
X-ray structure of SPOP MATH domain (D140G) in complex with a 53BP1 peptide
要素
Speckle-type POZ protein
TP53-binding protein 1 peptide
キーワード
PROTEIN BINDING / SPOP / 53BP1 / DNA damage response / Homologous recombination / Ubiquitin ligase
機能・相同性
機能・相同性情報
ubiquitin-modified histone reader activity / positive regulation of isotype switching / cellular response to X-ray / regulation of proteolysis / double-strand break repair via classical nonhomologous end joining / protein localization to site of double-strand break / DNA repair complex / Cul3-RING ubiquitin ligase complex / molecular function inhibitor activity / telomeric DNA binding ...ubiquitin-modified histone reader activity / positive regulation of isotype switching / cellular response to X-ray / regulation of proteolysis / double-strand break repair via classical nonhomologous end joining / protein localization to site of double-strand break / DNA repair complex / Cul3-RING ubiquitin ligase complex / molecular function inhibitor activity / telomeric DNA binding / SUMOylation of transcription factors / negative regulation of double-strand break repair via homologous recombination / histone reader activity / methylated histone binding / DNA damage checkpoint signaling / replication fork / Nonhomologous End-Joining (NHEJ) / transcription coregulator activity / Hedgehog 'on' state / protein homooligomerization / G2/M DNA damage checkpoint / kinetochore / positive regulation of DNA-binding transcription factor activity / double-strand break repair via nonhomologous end joining / protein polyubiquitination / p53 binding / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / site of double-strand break / Processing of DNA double-strand break ends / histone binding / proteasome-mediated ubiquitin-dependent protein catabolic process / RNA polymerase II-specific DNA-binding transcription factor binding / damaged DNA binding / chromosome, telomeric region / nuclear body / nuclear speck / ubiquitin protein ligase binding / DNA damage response / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / nucleoplasm / identical protein binding / nucleus / cytoplasm 類似検索 - 分子機能
温度: 295 K / 手法: 蒸気拡散法, ハンギングドロップ法 詳細: SPOP MATH was at 24 mg/ml and 1:5 protein:53BP1 peptide molar ratio. Crystals were grown by the hanging drop method, mixing 2 ul of the protein sample in 20 mM Tris-HCl, pH 7.6, 150 mM NaCl, ...詳細: SPOP MATH was at 24 mg/ml and 1:5 protein:53BP1 peptide molar ratio. Crystals were grown by the hanging drop method, mixing 2 ul of the protein sample in 20 mM Tris-HCl, pH 7.6, 150 mM NaCl, 5 mM DTT and 2 ul of the reservoir solution for the drop. The reservoir solution was 0.5 ml. Reservoir solution: 0.1 M sodium citrate tribasic dihydrate, pH 5.6, 0.2 M (NH4)2SO4, 1 M Li2SO4