Mass: 230.266 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Formula: C12H14N4O / Feature type: SUBJECT OF INVESTIGATION
Has ligand of interest
Y
-
Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
2D 1H-15N HSQC NH2 only
1
2
1
isotropic
1
2D 1H-13C HSQC aromatic
1
3
1
isotropic
2
3DCBCA(CO)NH
1
4
1
isotropic
2
3D HN(CA)CB
1
5
1
isotropic
2
3DC(CO)NH
1
6
1
isotropic
2
3DH(CCO)NH
1
7
1
isotropic
1
3D 1H-13C NOESY aliphatic
1
8
1
isotropic
1
3D 1H-15N NOESY aliphatic
1
9
1
isotropic
2
3D 1H-13C NOESY CNfiltered
1
10
1
isotropic
2
2D 1H-15N HSQC CNfiltered
-
Sample preparation
Details
Type: solution Contents: 0.5 mM [U-13C; U-15N] TroponinC-Troponin I chimera, 10 mM Imidazole, 100 mM KCl, 90% H2O/10% D2O Details: 100 mM KCl, 10 mM Imidazole, plus 50 uL 4.96 mM d6-DSS in D2O, 5 uL 1M CaCl2, and 10 uL 1M DTT, pH 6.7 Label: TnC-TnI-tirasemtiv / Solvent system: 90% H2O/10% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
0.5mM
TroponinC-Troponin I chimera
[U-13C; U-15N]
1
10mM
Imidazole
naturalabundance
1
100mM
KCl
naturalabundance
1
Sample conditions
Details: dissolved in NMR buffer (100 mM KCl, 10 mM Imidazole), plus 50 uL 4.96 mM d6-DSS in D2O, 5 uL 1M CaCl2, and 10 uL 1M DTT, pH 6.7 Ionic strength: 200 mM / Label: cond1 / pH: 6.7 / Pressure: 1 atm / Temperature: 298 K
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