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- PDB-7fj2: Structure of FOXM1 homodimer bound to a palindromic DNA site -

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Basic information

Entry
Database: PDB / ID: 7fj2
TitleStructure of FOXM1 homodimer bound to a palindromic DNA site
Components
  • DNA (5'-D(*AP*CP*CP*GP*TP*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*AP*CP*GP*GP*T)-3')
  • Forkhead box protein M1
KeywordsTRANSCRIPTION / Forkhead transcription factors / FOXM1 / Dimer / DNA binding
Function / homology
Function and homology information


regulation of Ras protein signal transduction / Polo-like kinase mediated events / negative regulation of stress-activated MAPK cascade / regulation of reactive oxygen species metabolic process / positive regulation of double-strand break repair / Cyclin A/B1/B2 associated events during G2/M transition / Nuclear events stimulated by ALK signaling in cancer / DNA damage response, signal transduction by p53 class mediator / RNA polymerase II transcription regulatory region sequence-specific DNA binding / G2/M transition of mitotic cell cycle ...regulation of Ras protein signal transduction / Polo-like kinase mediated events / negative regulation of stress-activated MAPK cascade / regulation of reactive oxygen species metabolic process / positive regulation of double-strand break repair / Cyclin A/B1/B2 associated events during G2/M transition / Nuclear events stimulated by ALK signaling in cancer / DNA damage response, signal transduction by p53 class mediator / RNA polymerase II transcription regulatory region sequence-specific DNA binding / G2/M transition of mitotic cell cycle / regulation of cell population proliferation / DNA-binding transcription factor activity, RNA polymerase II-specific / regulation of cell cycle / DNA-binding transcription factor activity / DNA repair / negative regulation of DNA-templated transcription / positive regulation of cell population proliferation / regulation of transcription by RNA polymerase II / protein kinase binding / chromatin / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus
Similarity search - Function
Forkhead box protein M1 / : / Fork head domain conserved site1 / Fork head domain signature 1. / Fork head domain / Forkhead domain / Fork head domain profile. / FORKHEAD / Fork head domain conserved site 2 / Fork head domain signature 2. ...Forkhead box protein M1 / : / Fork head domain conserved site1 / Fork head domain signature 1. / Fork head domain / Forkhead domain / Fork head domain profile. / FORKHEAD / Fork head domain conserved site 2 / Fork head domain signature 2. / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily
Similarity search - Domain/homology
DNA / DNA (> 10) / Forkhead box protein M1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 3.098 Å
AuthorsDai, S.Y. / Li, J. / Zhang, H.J.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)81570537 China
CitationJournal: J.Mol.Biol. / Year: 2021
Title: Mechanistic Insights into the Preference for Tandem Binding Sites in DNA Recognition by FOXM1.
Authors: Zhang, H. / Dai, S. / Liang, X. / Li, J. / Chen, Y.
History
DepositionAug 2, 2021Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jan 26, 2022Provider: repository / Type: Initial release
Revision 1.1Nov 29, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Forkhead box protein M1
B: Forkhead box protein M1
C: DNA (5'-D(*AP*CP*CP*GP*TP*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*AP*CP*GP*GP*T)-3')
D: DNA (5'-D(*AP*CP*CP*GP*TP*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*AP*CP*GP*GP*T)-3')
E: Forkhead box protein M1
F: Forkhead box protein M1
G: DNA (5'-D(*AP*CP*CP*GP*TP*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*AP*CP*GP*GP*T)-3')
H: DNA (5'-D(*AP*CP*CP*GP*TP*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*AP*CP*GP*GP*T)-3')


Theoretical massNumber of molelcules
Total (without water)79,2548
Polymers79,2548
Non-polymers00
Water00
1
A: Forkhead box protein M1
B: Forkhead box protein M1
C: DNA (5'-D(*AP*CP*CP*GP*TP*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*AP*CP*GP*GP*T)-3')
D: DNA (5'-D(*AP*CP*CP*GP*TP*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*AP*CP*GP*GP*T)-3')


Theoretical massNumber of molelcules
Total (without water)39,6274
Polymers39,6274
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5490 Å2
ΔGint-37 kcal/mol
Surface area13800 Å2
MethodPISA
2
E: Forkhead box protein M1
F: Forkhead box protein M1
G: DNA (5'-D(*AP*CP*CP*GP*TP*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*AP*CP*GP*GP*T)-3')
H: DNA (5'-D(*AP*CP*CP*GP*TP*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*AP*CP*GP*GP*T)-3')


Theoretical massNumber of molelcules
Total (without water)39,6274
Polymers39,6274
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5460 Å2
ΔGint-37 kcal/mol
Surface area14180 Å2
MethodPISA
Unit cell
Length a, b, c (Å)69.387, 45.456, 133.591
Angle α, β, γ (deg.)90.000, 92.850, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11chain C
21chain D
31chain G
41chain H
12(chain A and (resid 236 through 273 or (resid 274...
22(chain B and (resid 236 through 252 or (resid 253...
32(chain E and (resid 236 through 273 or (resid 274...
42(chain F and (resid 236 through 252 or (resid 253...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111DADADTDTchain CCC7 - 261 - 20
211DADADTDTchain DDD7 - 261 - 20
311DADADTDTchain GGG7 - 261 - 20
411DADADTDTchain HHH7 - 261 - 20
112ARGARGPHEPHE(chain A and (resid 236 through 273 or (resid 274...AA236 - 27316 - 53
122LYSLYSLYSLYS(chain A and (resid 236 through 273 or (resid 274...AA27454
132VALVALHISHIS(chain A and (resid 236 through 273 or (resid 274...AA233 - 31113 - 91
142VALVALHISHIS(chain A and (resid 236 through 273 or (resid 274...AA233 - 31113 - 91
152VALVALHISHIS(chain A and (resid 236 through 273 or (resid 274...AA233 - 31113 - 91
162VALVALHISHIS(chain A and (resid 236 through 273 or (resid 274...AA233 - 31113 - 91
212ARGARGTHRTHR(chain B and (resid 236 through 252 or (resid 253...BB236 - 25216 - 32
222GLUGLUARGARG(chain B and (resid 236 through 252 or (resid 253...BB253 - 25433 - 34
232ARGARGHISHIS(chain B and (resid 236 through 252 or (resid 253...BB236 - 31116 - 91
242ARGARGHISHIS(chain B and (resid 236 through 252 or (resid 253...BB236 - 31116 - 91
252ARGARGHISHIS(chain B and (resid 236 through 252 or (resid 253...BB236 - 31116 - 91
262ARGARGHISHIS(chain B and (resid 236 through 252 or (resid 253...BB236 - 31116 - 91
312ARGARGPHEPHE(chain E and (resid 236 through 273 or (resid 274...EE236 - 27316 - 53
322LYSLYSLYSLYS(chain E and (resid 236 through 273 or (resid 274...EE27454
332VALVALHISHIS(chain E and (resid 236 through 273 or (resid 274...EE233 - 31113 - 91
342VALVALHISHIS(chain E and (resid 236 through 273 or (resid 274...EE233 - 31113 - 91
352VALVALHISHIS(chain E and (resid 236 through 273 or (resid 274...EE233 - 31113 - 91
362VALVALHISHIS(chain E and (resid 236 through 273 or (resid 274...EE233 - 31113 - 91
412ARGARGTHRTHR(chain F and (resid 236 through 252 or (resid 253...FF236 - 25216 - 32
422GLUGLUARGARG(chain F and (resid 236 through 252 or (resid 253...FF253 - 25433 - 34
432SERSERHISHIS(chain F and (resid 236 through 252 or (resid 253...FF232 - 31112 - 91
442SERSERHISHIS(chain F and (resid 236 through 252 or (resid 253...FF232 - 31112 - 91
452SERSERHISHIS(chain F and (resid 236 through 252 or (resid 253...FF232 - 31112 - 91
462SERSERHISHIS(chain F and (resid 236 through 252 or (resid 253...FF232 - 31112 - 91

NCS ensembles :
ID
1
2

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Components

#1: Protein
Forkhead box protein M1


Mass: 13680.487 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FOXM1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q08050
#2: DNA chain
DNA (5'-D(*AP*CP*CP*GP*TP*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*AP*CP*GP*GP*T)-3')


Mass: 6132.991 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli)

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.65 Å3/Da / Density % sol: 53.67 %
Description: THE ENTRY CONTAINS FRIEDEL PAIRS IN I_PLUS/MINUS COLUMNS.
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop / pH: 4.7
Details: 50 mM Sodium acetate pH 4.7 250 mM Sodium chloride 15% PEG 3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: ROTATING ANODE / Type: RIGAKU MICROMAX-007 HF / Wavelength: 1.58 Å
DetectorType: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Dec 20, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.58 Å / Relative weight: 1
ReflectionResolution: 3.098→49.303 Å / Num. obs: 12865 / % possible obs: 87.7 % / Redundancy: 5.2 % / Biso Wilson estimate: 73.27 Å2 / CC1/2: 0.978 / CC star: 0.994 / Net I/σ(I): 10.1
Reflection shellResolution: 3.1→3.2 Å / Num. unique obs: 810 / CC1/2: 0.946

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Processing

Software
NameVersionClassification
HKL-2000data reduction
HKL-2000data scaling
PHENIX1.11.1_2575refinement
PDB_EXTRACT3.27data extraction
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3G73

3g73


Resolution: 3.098→49.303 Å / SU ML: 0.33 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 27.57 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2654 1274 9.91 %
Rwork0.216 11578 -
obs0.2208 12852 82.43 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 96.65 Å2 / Biso mean: 65.6844 Å2 / Biso min: 44.72 Å2
Refinement stepCycle: final / Resolution: 3.098→49.303 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2556 1624 0 0 4180
Num. residues----394
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0084464
X-RAY DIFFRACTIONf_angle_d0.9046400
X-RAY DIFFRACTIONf_chiral_restr0.05691
X-RAY DIFFRACTIONf_plane_restr0.007529
X-RAY DIFFRACTIONf_dihedral_angle_d20.4652308
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11C780X-RAY DIFFRACTION8.624TORSIONAL
12D780X-RAY DIFFRACTION8.624TORSIONAL
13G780X-RAY DIFFRACTION8.624TORSIONAL
14H780X-RAY DIFFRACTION8.624TORSIONAL
21A1435X-RAY DIFFRACTION8.624TORSIONAL
22B1435X-RAY DIFFRACTION8.624TORSIONAL
23E1435X-RAY DIFFRACTION8.624TORSIONAL
24F1435X-RAY DIFFRACTION8.624TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
3.098-3.22160.401910.314680153
3.2216-3.36820.30551070.281396162
3.3682-3.54580.30941240.2608108971
3.5458-3.76780.29381360.2552127381
3.7678-4.05860.27141460.2324136188
4.0586-4.46680.24221600.2013145494
4.4668-5.11260.2661610.2002152897
5.1126-6.43910.24571800.1915155698
6.4391-49.3030.24331690.197155595
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.550.1299-0.14190.1247-0.15080.09970.4781-0.77780.75960.5404-0.56260.13150.25361.39760.01750.7430.0540.10540.824-0.32960.5871-54.170310.7-21.5468
20.0527-0.05680.08630.17770.40650.86410.0468-0.5506-0.43120.98020.1335-0.171-0.81310.5637-0.00010.6079-0.0188-0.04440.6751-0.05520.6196-42.935715.0133-30.8085
30.6813-0.77480.11190.8325-0.12660.01250.5088-0.43120.0567-0.20850.1803-0.301-0.35320.54330.00230.58390.1345-0.02450.72020.00250.6233-42.16676.2378-31.0272
40.8003-0.3390.24590.38780.15250.50950.1545-0.614-0.5482-0.4485-0.01480.2012-0.1231-0.8668-0.00010.5488-0.04040.01780.6125-0.02240.6037-52.56655.8689-32.4727
50.2246-0.1074-0.19470.1855-0.27750.7862-0.90050.6411-0.77830.3080.51851.55630.2954-0.2237-0.00420.7301-0.04870.07860.52420.10290.6705-43.301116.2975-42.0655
65.2134-1.6834-0.39069.6677-0.96190.16961.18530.3652-0.55040.69280.73870.8465-0.50920.86020.61470.69230.0057-0.68610.6681-0.022-0.6898-36.222510.5643-42.1304
72.0025-3.20843.86055.4898-5.5189.2758-0.53860.46780.2727-0.3627-0.7182-1.5781-1.0643-0.3259-2.06370.75360.14160.08560.1814-0.39750.9893-37.6575-6.0879-72.2042
82.0028-1.6459-0.53231.7772-0.41060.18320.34612.03631.1793-0.40910.3486-0.62880.42630.0870.08420.82230.01910.02180.5752-0.13750.252-34.61031.8431-65.8925
90.76790.5648-0.34490.327-0.32880.74790.4210.15480.5510.30080.11711.218-0.2376-0.28380.01290.55080.0264-0.09880.56560.07720.4637-42.45285.3258-67.1367
100.89920.1141-1.25331.1595-0.11251.5336-0.077-0.1758-0.27841.0477-0.2407-0.19910.4194-0.2632-0.04550.7862-0.05680.10360.57620.03480.4111-38.7741-0.6259-56.8971
110.0972-0.025-0.13680.10390.15940.17840.39630.16450.34080.088-0.1337-0.2826-0.0079-0.2076-0.00040.68180.02040.11330.4241-0.06890.3518-36.716711.4762-53.8282
120.87881.1545-0.1231.25140.2334-0.04790.08820.49720.13650.346-0.49860.1882-0.0444-1.1926-0.00120.82610.08710.04290.746-0.00010.8815-59.750510.1486-41.4708
130.21731.30510.4012.18711.11340.16970.01310.2978-0.07450.03020.0941-0.55260.2111-0.2065-0.00090.80960.04050.02970.49210.02060.5125-41.6744-11.5076-54.4893
140.1774-0.1190.09010.12110.0530.1239-0.9326-1.8343-0.2020.45380.91370.63120.0092-0.4576-0.00120.98990.06790.0540.71320.01860.592-36.4772-13.7046-61.1058
15-0.0309-0.20990.18250.6175-0.42650.215-0.16940.20450.32210.4263-0.20710.46870.3527-0.2086-0.00020.701-0.03380.03750.5616-0.07380.6428-50.38850.1497-48.2337
160.2209-0.0516-0.22290.01080.14560.24961.54370.81761.5570.54690.5836-0.6787-0.7793-0.00360.00480.77360.1506-0.01141.2793-0.22491.0112-68.188714.4387-37.8804
171.79771.32650.33591.5496-0.24810.38870.05471.3432-1.5767-0.1466-0.3282-1.3056-0.6532-0.42940.03250.8880.1310.25130.80510.05230.667112.7089-9.6071-18.5874
181.0676-1.5278-2.90863.1843.07912.00770.4622-0.447-0.9831-0.56360.0206-0.0744-0.59211.19651.2215-0.15820.2542-0.3390.72960.20761.10762.9258-14.1804-8.1919
190.78820.87310.00751.1522-0.31440.2431-0.2037-0.2610.35330.7110.0838-0.5761-0.91920.7317-0.00110.4862-0.0537-0.12030.89480.07210.49264.8904-3.9697-10.8943
200.0592-0.07670.08030.41710.04980.175-0.53670.50820.93870.98040.8437-0.60380.317-0.4265-0.00290.43170.0565-0.0620.54540.06330.9886-3.3829-7.2714-18.1192
210.72350.21090.22170.09080.36381.26050.01410.0211-0.1271-0.01470.19130.25931.1145-0.42570.0390.6099-0.06780.06370.67690.27531.1838-8.2403-15.7427-9.2747
220.0981-0.39310.05991.1782-0.26610.0276-0.70590.11710.7559-1.16960.7206-0.91820.6288-0.635-0.00470.397-0.03530.04930.6826-0.14760.8408-8.9356-10.0052-2.502
232.0191.94631.96510.35850.6623.0788-0.95471.18091.6580.6294-0.3695-1.26790.2907-0.3003-0.6120.50210.2035-0.04570.5124-0.12280.5996-42.2027.7103-10.5381
243.22020.0517-1.01870.6743-1.19981.8705-0.1655-0.0236-0.2153-0.41330.0639-0.26410.3708-0.37720.00050.34410.0044-0.0620.47010.03370.4105-31.5666-2.0744-9.2591
250.4177-0.78220.91221.4916-1.73932.01380.0899-0.4032-0.19311.2511-0.8487-0.8222-0.64811.1064-0.18740.5574-0.075-0.07220.64570.30810.8993-21.9072-3.1928-0.0135
260.08170.0772-0.06250.0649-0.02140.0076-0.3498-0.0776-0.5449-0.0973-0.0640.33620.54490.38260.00090.37430.00410.06880.4560.07890.4657-21.154-10.9526-3.9353
270.5991-0.1828-0.63910.6338-0.791.01180.28220.54060.46640.2672-0.2067-0.42540.258-0.3151-0.00050.92220.08820.17030.71360.02360.6151-6.6602-9.5192-25.8549
280.1898-0.50850.25882.1561-1.62990.7892-0.35680.61890.32060.32560.1052-0.4970.0947-0.67990.00010.5773-0.0250.03460.6286-0.02910.8808-21.095111.9013-8.5268
290.1484-0.0862-0.45440.1981-0.24780.7777-0.0516-0.25850.84640.1255-0.2242-0.19760.26510.10370.00060.4732-0.04920.05970.584-0.01090.7171-21.775111.5165-9.9227
301.64750.8629-2.46430.4312-1.30882.3954-0.3140.63160.35450.21630.1615-0.65930.4583-0.1826-0.00070.68970.15320.06780.7404-0.00910.7535-7.3832-11.0169-26.2744
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 233 through 240 )A233 - 240
2X-RAY DIFFRACTION2chain 'A' and (resid 241 through 255 )A241 - 255
3X-RAY DIFFRACTION3chain 'A' and (resid 256 through 269 )A256 - 269
4X-RAY DIFFRACTION4chain 'A' and (resid 270 through 291 )A270 - 291
5X-RAY DIFFRACTION5chain 'A' and (resid 292 through 299 )A292 - 299
6X-RAY DIFFRACTION6chain 'A' and (resid 300 through 311 )A300 - 311
7X-RAY DIFFRACTION7chain 'B' and (resid 236 through 240 )B236 - 240
8X-RAY DIFFRACTION8chain 'B' and (resid 241 through 251 )B241 - 251
9X-RAY DIFFRACTION9chain 'B' and (resid 252 through 278 )B252 - 278
10X-RAY DIFFRACTION10chain 'B' and (resid 279 through 299 )B279 - 299
11X-RAY DIFFRACTION11chain 'B' and (resid 300 through 311 )B300 - 311
12X-RAY DIFFRACTION12chain 'C' and (resid 7 through 16 )C7 - 16
13X-RAY DIFFRACTION13chain 'C' and (resid 17 through 26 )C17 - 26
14X-RAY DIFFRACTION14chain 'D' and (resid 7 through 11 )D7 - 11
15X-RAY DIFFRACTION15chain 'D' and (resid 12 through 21 )D12 - 21
16X-RAY DIFFRACTION16chain 'D' and (resid 22 through 26 )D22 - 26
17X-RAY DIFFRACTION17chain 'E' and (resid 233 through 240 )E233 - 240
18X-RAY DIFFRACTION18chain 'E' and (resid 241 through 255 )E241 - 255
19X-RAY DIFFRACTION19chain 'E' and (resid 256 through 278 )E256 - 278
20X-RAY DIFFRACTION20chain 'E' and (resid 279 through 291 )E279 - 291
21X-RAY DIFFRACTION21chain 'E' and (resid 292 through 299 )E292 - 299
22X-RAY DIFFRACTION22chain 'E' and (resid 300 through 311 )E300 - 311
23X-RAY DIFFRACTION23chain 'F' and (resid 232 through 240 )F232 - 240
24X-RAY DIFFRACTION24chain 'F' and (resid 241 through 291 )F241 - 291
25X-RAY DIFFRACTION25chain 'F' and (resid 292 through 299 )F292 - 299
26X-RAY DIFFRACTION26chain 'F' and (resid 300 through 311 )F300 - 311
27X-RAY DIFFRACTION27chain 'G' and (resid 7 through 16 )G7 - 16
28X-RAY DIFFRACTION28chain 'G' and (resid 17 through 26 )G17 - 26
29X-RAY DIFFRACTION29chain 'H' and (resid 7 through 16 )H7 - 16
30X-RAY DIFFRACTION30chain 'H' and (resid 17 through 26 )H17 - 26

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