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Open data
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Basic information
Entry | Database: PDB / ID: 7dik | ||||||
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Title | Structure of PfGrx1 with barium | ||||||
![]() | Glutaredoxin | ||||||
![]() | OXIDOREDUCTASE / REDOX ENZYME / TRX FOLD / GLUTATHIONE / Ba-SAD | ||||||
Function / homology | ![]() protein-disulfide reductase (glutathione) activity / Interconversion of nucleotide di- and triphosphates / glutathione disulfide oxidoreductase activity / antioxidant activity / mitochondrion / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Manickam, Y. / Sharma, A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Interaction of metals with PfGrx1 Authors: Manickam, Y. / Sharma, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 85.4 KB | Display | ![]() |
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PDB format | ![]() | 67.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 985 KB | Display | ![]() |
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Full document | ![]() | 985.1 KB | Display | |
Data in XML | ![]() | 8.2 KB | Display | |
Data in CIF | ![]() | 11.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7dilC ![]() 7dimC ![]() 7dinC ![]() 7dioC ![]() 7dipC ![]() 7diqC ![]() 7dirC ![]() 7disC ![]() 7ditC ![]() 7diuC ![]() 7divC ![]() 7diwC ![]() 7dizC ![]() 7dj0C C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 12436.457 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: isolate 3D7 / Gene: PF3D7_0306300 / Plasmid: PQE30 / Production host: ![]() ![]() References: UniProt: Q9NLB2, protein-disulfide reductase (glutathione) | ||||
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#2: Chemical | ChemComp-MPO / | ||||
#3: Chemical | ChemComp-MPD / ( | ||||
#4: Chemical | ChemComp-BA / #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45.09 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 12.5%(W/V) PEG1000, 12.5%(W/V) PEG3350, 12.5%(V/V) MPD, 0.02M AMINO ACIDS, 0.1M MOPS/HEPES SODIUM |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Jul 2, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.55→50 Å / Num. obs: 16793 / % possible obs: 99.9 % / Redundancy: 7.7 % / Rmerge(I) obs: 0.043 / Net I/σ(I): 896.1 |
Reflection shell | Resolution: 1.55→1.58 Å / Redundancy: 6.8 % / Rmerge(I) obs: 0.337 / Mean I/σ(I) obs: 42.1 / Num. unique obs: 800 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 81.98 Å2 / Biso mean: 21.0734 Å2 / Biso min: 10.42 Å2 | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.55→24.112 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / % reflection obs: 100 %
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