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- PDB-7dfo: Crystal structure of glycoside hydrolase family 11 beta-xylanase ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7dfo | ||||||
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Title | Crystal structure of glycoside hydrolase family 11 beta-xylanase from Streptomyces olivaceoviridis E-86 in complex with 4-O-methyl-alpha-D-glucuronopyranosyl xylotetraose | ||||||
![]() | Endo-1,4-beta-xylanase | ||||||
![]() | HYDROLASE / glycoside hydrolase family 11 | ||||||
Function / homology | ![]() endo-1,4-beta-xylanase activity / endo-1,4-beta-xylanase / xylan catabolic process Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fujimoto, Z. / Kishine, N. / Kaneko, S. | ||||||
![]() | ![]() Title: Structure-based substrate specificity analysis of GH11 xylanase from Streptomyces olivaceoviridis E-86. Authors: Fujimoto, Z. / Kishine, N. / Teramoto, K. / Tsutsui, S. / Kaneko, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 373.9 KB | Display | ![]() |
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PDB format | ![]() | 295.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.2 MB | Display | ![]() |
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Full document | ![]() | 2.2 MB | Display | |
Data in XML | ![]() | 68.5 KB | Display | |
Data in CIF | ![]() | 99.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7dfmSC ![]() 7dfnC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
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Components
-Protein , 1 types, 9 molecules ABCDEFGHI
#1: Protein | Mass: 22537.367 Da / Num. of mol.: 9 Source method: isolated from a genetically manipulated source Details: GB LC578783 Source: (gene. exp.) ![]() Strain: E-86 / Gene: soxB / Production host: ![]() ![]() References: UniProt: A0A7G1MBT0*PLUS, endo-1,4-beta-xylanase |
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-Sugars , 4 types, 8 molecules
#2: Polysaccharide | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose #3: Polysaccharide | #4: Polysaccharide | 4-O-methyl-alpha-D-glucopyranuronic acid-(1-2)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)- ...4-O-methyl-alpha-D-glucopyranuronic acid-(1-2)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose | #5: Polysaccharide | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose | |
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-Non-polymers , 3 types, 904 molecules ![](data/chem/img/CL.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
#6: Chemical | ChemComp-CL / #7: Chemical | ChemComp-NA / #8: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 41.43 % / Description: rod |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.4 Details: 2.9 M sodium chloride, 0.1 M sodium citrate buffer pH 5.4 |
-Data collection
Diffraction | Mean temperature: 95 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 25, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2→100 Å / Num. obs: 110677 / % possible obs: 99.3 % / Redundancy: 8.7 % / Rmerge(I) obs: 0.165 / Net I/σ(I): 17.9 |
Reflection shell | Resolution: 2→2.07 Å / Rmerge(I) obs: 0.839 / Mean I/σ(I) obs: 2.3 / Num. unique obs: 11151 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 7DFM Resolution: 2→47.061 Å / Cor.coef. Fo:Fc: 0.966 / Cor.coef. Fo:Fc free: 0.942 / SU B: 4.858 / SU ML: 0.131 / Cross valid method: FREE R-VALUE / ESU R: 0.19 / ESU R Free: 0.166 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 30.941 Å2
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Refinement step | Cycle: LAST / Resolution: 2→47.061 Å
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Refine LS restraints |
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LS refinement shell |
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