PDB-7d9x: Highly active mutant W525D of Gamma-glutamyltranspeptidase from P...

ID or keywords:

Entry

Database: PDB / ID: 7d9x

Title

Highly active mutant W525D of Gamma-glutamyltranspeptidase from Pseudomonas nitroreducens

Components

(Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family ...) x 2

Keywords

TRANSFERASE / theanine synthesis / transpeptidation / enzyme / protein engineering / substrate specificity / reaction specificity

Function / homology

Function and homology information

hypoglycin A gamma-glutamyl transpeptidase activity / glutathione hydrolase activity / leukotriene C4 gamma-glutamyl transferase activity / glutathione catabolic process
Similarity search - Function

Biological species

Pseudomonas nitroreducens (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.74 Å

Authors

Hibi, T. / Sano, C. / Itoh, T. / Wakayama, M.

Citation

Journal: Biochem.Biophys.Res.Commun. / Year: 2021
Title: Mutagenesis and structure-based analysis of the role of Tryptophan525 of gamma-glutamyltranspeptidase from Pseudomonas nitroreducens.
Authors: Sano, C. / Itoh, T. / Phumsombat, P. / Hayashi, J. / Wakayama, M. / Hibi, T.

History

Deposition	Oct 14, 2020	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Jun 23, 2021	Provider: repository / Type: Initial release
Revision 1.1	Nov 29, 2023	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	7d9x.cif.gz	653.5 KB	Display	PDBx/mmCIF format
PDB format	pdb7d9x.ent.gz	492.1 KB	Display	PDB format
PDBx/mmJSON format	7d9x.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	7d9x_validation.pdf.gz	2.3 MB	Display	wwPDB validaton report
Full document	7d9x_full_validation.pdf.gz	2.3 MB	Display
Data in XML	7d9x_validation.xml.gz	47.1 KB	Display
Data in CIF	7d9x_validation.cif.gz	70.2 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/d9/7d9x ftp://data.pdbj.org/pub/pdb/validation_reports/d9/7d9x	HTTPS FTP

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Related structure data

Related structure data	7d9eC 7d9wC 5zjgS S: Starting model for refinement C: citing same article (ref.)
Similar structure data	4kt6-2 4kt6-1 2zpe-d 5zjg-2 5bpk-1 2nqo-3 3rix-d 1cby-1 4puj-d 6b0f-d Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#177 - Sep 2014 Apoptosomes similarity (1) #166 - Oct 2013 Proteasome similarity (1) #80 - Aug 2006 AAA+ Proteases similarity (1) #235 - Jul 2019 AMPA Receptor similarity (1) #176 - Aug 2014 Dynein similarity (1) #60 - Dec 2004 Ubiquitin similarity (1) #142 - Oct 2011 PDB Pioneers similarity (1)

Deposited unit

A: Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family T03

B: Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family T03

C: Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family T03

D: Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family T03

hetero molecules

123 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family T03

B: Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family T03

hetero molecules

defined by author&software
Evidence: native gel electrophoresis
61.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

C: Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family T03

D: Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family T03

hetero molecules

defined by author&software
61.4 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	49.624, 117.189, 187.589
Angle α, β, γ (deg.)	90.000, 90.000, 90.000
Int Tables number	19
Space group name H-M	P2₁2₁2₁
Space group name Hall	P2ac2ab
Symmetry operation	#1: x,y,z #2: x+1/2,-y+1/2,-z #3: -x,y+1/2,-z+1/2 #4: -x+1/2,-y,z+1/2

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Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family ... , 2 types, 4 molecules ACBD

#1: Protein	Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family T03 Mass: 40283.793 Da / Num. of mol.: 2 / Fragment: L-subunit Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas nitroreducens (bacteria) / Gene: SAMN05216209_3923 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A239KXH0
#2: Protein	Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family T03 Mass: 20754.340 Da / Num. of mol.: 2 / Fragment: S-subunit / Mutation: W525D Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas nitroreducens (bacteria) / Gene: SAMN05216209_3923 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A239KXH0

#1: Protein

Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family T03

Mass: 40283.793 Da / Num. of mol.: 2 / Fragment: L-subunit
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Pseudomonas nitroreducens (bacteria) / Gene: SAMN05216209_3923 / Production host:

Escherichia coli (E. coli) / References: UniProt: A0A239KXH0

#2: Protein

Gamma-glutamyltransferase 1 Threonine peptidase. MEROPS family T03

Mass: 20754.340 Da / Num. of mol.: 2 / Fragment: S-subunit / Mutation: W525D
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Pseudomonas nitroreducens (bacteria) / Gene: SAMN05216209_3923 / Production host:

Escherichia coli (E. coli) / References: UniProt: A0A239KXH0

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Non-polymers , 4 types, 925 molecules

#3: Chemical	ChemComp-GBL / GAMMA-BUTYROLACTONE / DIHYDROFURAN-2(3H)-ONE Mass: 86.089 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C₄H₆O₂
#4: Chemical	ChemComp-GLY / GLYCINE Type: peptide linking / Mass: 75.067 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₂H₅NO₂ / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL Mass: 92.094 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: C₃H₈O₃
#6: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 916 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.27 Å³/Da / Density % sol: 45.92 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 16% PEG 8000, 15% PEG 400, 0.1M HEPES pH 7, 50mM Gly-Gly

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL38B1 / Wavelength: 1 Å
Detector	Type: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 10, 2016
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 1.74→46.9 Å / Num. obs: 111797 / % possible obs: 99.5 % / Redundancy: 13.8 % / B_iso Wilson estimate: 24.67 Å² / R_merge(I) obs: 0.09 / Net I/σ(I): 47.9
Reflection shell	Resolution: 1.74→1.79 Å / R_merge(I) obs: 1.05 / Mean I/σ(I) obs: 2.7 / Num. unique obs: 5485 / % possible all: 99.4

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Processing

Software

Name	Version	Classification
PHENIX	1.18.2_3874	refinement
PHENIX	1.18.2_3874	refinement
HKL-2000		data reduction
HKL-3000		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 5ZJG

5zjg

Resolution: 1.74→46.9 Å / SU ML: 0.1473 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 18.8681
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.187	1997	1.79 %
R_work	0.1669	109629	-
obs	0.1673	111626	99.37 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 32.12 Å²

Refinement step

Cycle: LAST / Resolution: 1.74→46.9 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	7851	0	50	916	8817

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0077	8136
X-RAY DIFFRACTION	f_angle_d	0.8469	11060
X-RAY DIFFRACTION	f_chiral_restr	0.0539	1225
X-RAY DIFFRACTION	f_plane_restr	0.0054	1460
X-RAY DIFFRACTION	f_dihedral_angle_d	14.763	2875

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.74-1.79	0.2548	134	0.2309	7356	X-RAY DIFFRACTION	94.34
1.79-1.84	0.2791	142	0.2247	7774	X-RAY DIFFRACTION	99.75
1.84-1.89	0.2538	141	0.2168	7746	X-RAY DIFFRACTION	99.82
1.89-1.95	0.2369	141	0.1977	7768	X-RAY DIFFRACTION	99.9
1.95-2.02	0.1921	143	0.1869	7835	X-RAY DIFFRACTION	99.91
2.02-2.1	0.2161	142	0.1851	7778	X-RAY DIFFRACTION	99.97
2.1-2.2	0.2003	142	0.1757	7802	X-RAY DIFFRACTION	99.96
2.2-2.31	0.208	143	0.1651	7825	X-RAY DIFFRACTION	99.82
2.31-2.46	0.1773	143	0.165	7857	X-RAY DIFFRACTION	99.83
2.46-2.65	0.1858	144	0.1653	7837	X-RAY DIFFRACTION	99.66
2.65-2.91	0.1955	142	0.1654	7892	X-RAY DIFFRACTION	99.52
2.91-3.34	0.1937	143	0.1659	7908	X-RAY DIFFRACTION	99.59
3.34-4.2	0.1821	147	0.1475	7979	X-RAY DIFFRACTION	99.73
4.2-46.9	0.1467	150	0.1563	8272	X-RAY DIFFRACTION	99.29

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.868577694259	0.157226647468	0.271056036448	0.986910231225	-0.53136746706	1.63794087453	0.00154155345132	0.0523784134197	-0.00950530228025	-0.157031036681	0.0584001469	0.0283080384809	-0.0405816513508	-0.0431566688361	-0.0666071867043	0.246881591243	0.0222691753366	0.001141056028	0.189491524268	0.0121545869405	0.20486871487	1.38441480348	-14.0595808158	-32.0773486807
2	1.16247699205	0.101848579755	0.486422128931	1.52277967333	-0.622045227502	2.76307544968	0.0442640660139	0.119585821414	-0.0977941234949	-0.222659872918	0.0616396704304	-0.0504068501666	-0.0249921856914	0.101444452755	-0.12109862457	0.230451788641	0.0274086808857	0.0142941314791	0.188751714708	-0.0196134693081	0.198909599232	3.7200873599	-19.5899082273	-35.7921762799
3	1.00443885451	0.153760521837	0.141541691395	1.48086664147	-1.06350823391	2.88959116965	-0.0289041112289	0.0163427318452	0.10817182061	-0.19989963233	0.129913013435	0.314114727041	-0.478218992918	-0.611071270926	-0.082553573822	0.402663140136	0.0705217171946	-0.0906455415992	0.348493257549	0.0326963192875	0.388223989397	-15.313179882	-15.6699679389	-37.4299809567
4	0.817431353152	-0.352724278472	0.953557865151	2.43095815925	-0.912040286138	2.12173905311	0.0159982365954	-0.0777458668088	-0.165846976712	-0.447375456686	0.259868369557	0.472404600899	0.118480262086	-0.426142380513	-0.217109213108	0.350177089111	-0.00764970554285	-0.126986960087	0.378290782161	0.0412232628186	0.344335139971	-16.623669611	-19.2093357939	-43.3139995148
5	1.90887333544	0.338476924164	0.208147186914	1.15575181351	-0.429314720114	2.99509479279	0.0853176729264	0.242916207262	-0.317307425366	-0.587135989454	0.0853646569011	-0.00355599999873	0.285376250211	0.106574076346	-0.169370923825	0.389338986163	0.0334512461838	-0.0599735727963	0.22459186765	-0.0600685402278	0.302030641429	-0.169505303939	-28.8320477748	-41.9324142809
6	0.931000034479	0.291981784831	0.798762323322	1.01521937661	-0.483248184562	1.43220546693	-0.115346362312	0.0778829468972	0.152479346815	-0.134543583521	0.060312840304	-0.245549496524	-0.652946730765	0.316827647771	0.0531472147126	0.395440313843	-0.0736791440608	0.0199804527988	0.210356317853	0.0245190392918	0.186297648568	9.73865497951	-0.145951936167	-30.376758396
7	0.809346770306	-0.00493153441587	0.046181157224	1.27424857421	-0.165124432948	1.06225102843	-0.160927630219	0.147320910447	0.368799584716	-0.215640665049	0.0815308180028	-0.223637473242	-0.794880466563	0.0609561154089	0.0305013674515	0.850003281932	-0.106334661786	-0.0503560512009	0.248014392345	0.0717722403392	0.29283410705	6.39226896194	12.8918996786	-35.9922647976
8	0.272459334349	-0.363272404762	0.239983134433	1.54531285786	-0.146386102193	0.311761416887	-0.0224150515487	0.204347881098	0.322962194836	-0.422349999278	-0.102971193588	-0.378925148719	-1.010161778	0.512459840393	0.179079146877	0.848015372171	-0.296854210784	0.0232022396288	0.35016610038	0.101564335205	0.328135579333	15.6750442716	10.701464365	-37.7088414555
9	0.986356508767	0.00238242360513	0.390709489005	1.32430696253	0.0329239408769	1.92564335289	-0.0180082452025	0.196344607431	0.0685434650894	-0.340439360337	0.0524543033322	-0.10907168048	-0.361208329194	0.242381539588	-0.0454092161821	0.340794168941	-0.000594481565992	0.0285603091977	0.21968602194	0.0220393268971	0.202657178813	3.13288904468	-8.57528026386	-37.6774445539
10	1.32539973856	0.544481055571	-0.134815700353	1.95934592062	-0.392966371227	0.911569117629	-0.224538423678	0.0629541045821	0.289634747102	-0.309805932706	0.125693536239	0.232057479528	-0.839496511209	-0.275205127269	-0.00846523124204	0.640936150418	0.11057571226	-0.0811977015799	0.2329576553	0.0255501400203	0.253200830635	-4.11861132303	8.38295428198	-30.8435355132
11	2.84527587887	0.249694283836	1.76363835523	1.14152955111	0.0984325579228	3.97543402764	-0.0562094422677	-0.39579980644	-0.0352223797671	0.216234721403	0.101845491078	0.110456346406	-0.368133991576	-0.470661311828	-0.0451697101714	0.294263181252	0.109298542605	0.0461329242631	0.305841298309	0.0168138643327	0.22260721119	-6.40261436649	-6.58384823166	-17.250287883
12	2.09328744949	0.248558390235	-0.151370197019	2.68386773421	-0.0100889407355	2.46134788961	0.0568067996373	0.125862893326	0.270307884709	-0.175925727961	-0.0209643125898	-0.127574528774	-0.270828946992	0.0393346562578	-0.0300336509852	0.175890763902	-0.00423132734021	-0.0122274446251	0.191989301099	0.00648436339421	0.213177220674	-9.83964822911	21.1164327946	-88.5014902686
13	0.911335984021	-0.275816436217	0.253153939042	2.05637944028	0.531613489862	1.3347480581	0.0350415634503	-0.125259604806	-0.0813436800008	0.344860321187	0.0408928322957	-0.0590747595665	0.142673118799	-0.0487049646459	-0.0525016297631	0.123263453531	-0.0144239564883	-0.03957830066	0.200349676941	-0.0100631239889	0.169757040386	-13.2693707813	6.49634004071	-73.2369479968
14	0.562278032989	-0.857369922082	-0.0992186521479	1.51080494574	-0.201272173768	1.45385395661	0.0322231342184	-0.17342022057	-0.0797898649694	0.479857494981	-0.00139541267838	-0.15809483444	0.0197286591749	-0.0557561058101	-0.0152379062292	0.149470557638	-0.00607464323218	-0.0240533457134	0.231620899036	-0.0196510625346	0.159393475345	-13.9546869813	10.8957082379	-69.8461231424
15	1.88397027053	0.066472700606	0.28916633121	1.6699042794	0.268486858321	1.13439668889	-0.00181170499695	-0.0537144756577	0.00736411854949	0.129029002967	-0.091493373896	0.305582657538	-0.107584396505	-0.31410207936	0.0553254384698	0.111109567015	0.00977522001484	0.0220894526623	0.270511229901	-0.0626982904585	0.238677949302	-27.1303704399	15.5315533481	-76.245187794
16	1.00637927378	-0.299447228524	-0.341947185406	2.31038437007	0.6430682161	1.69209529835	0.0352169168962	-0.251362230144	0.01287921375	0.496097698044	0.0499141929912	-0.192881331014	0.131179747031	0.0937920089289	-0.0704613846381	0.207826413988	-0.0205751562575	-0.0769844960045	0.233801537157	0.00145689851205	0.190966259457	-9.97535832669	5.58444738831	-69.6638043175
17	1.86841637516	-0.4404558959	0.871397864821	1.50925448077	-0.0726973892025	2.36147164821	0.148768528992	0.241584204281	-0.193417439413	-0.251964814898	-0.0272557142004	-0.425950637327	0.260886516063	0.265889991003	-0.133218365522	0.240119526052	0.0440498462356	0.0197629293383	0.243228241164	-0.0543453278334	0.3660645491	-1.19820974752	-11.4962721785	-93.6111545074
18	1.37484015796	-0.135318434384	0.764732011113	1.51415999524	-0.380685832196	3.18979160708	0.0841915648774	-0.079501168453	-0.311599813327	0.0254742390047	0.0584230351111	-0.210658110468	0.497024671893	0.0227557892214	-0.158757261899	0.263174136382	0.0160108544667	-0.0812231500038	0.201711460833	0.00153866497461	0.393897504167	-7.27232100925	-15.4067817559	-86.4448165803
19	1.26619971121	0.0302314526935	0.597829229028	1.50097922601	0.548021125785	1.87980878839	0.179298432995	-0.0948813182242	-0.479387188691	0.261232659959	0.0561248601257	-0.393027496464	0.442668154405	0.161793924761	-0.222839954386	0.325507465308	0.0461664164391	-0.125905648975	0.238195967195	0.0162845236345	0.471583023099	-1.15744258806	-16.8459194617	-78.9499794486
20	1.16679234877	-0.0930189445799	-0.125436668747	1.54220468234	0.494388442377	1.89787106987	0.0609813770924	-0.145773144898	-0.143696735178	0.284847899833	-0.0544912281853	0.0241791958371	0.312232219985	-0.0926541627599	-0.0204065797019	0.17655004319	-0.0189701127273	-0.0362241205826	0.21661394674	-0.000168834243776	0.193743624727	-16.2694608207	2.22792516891	-74.3914610672
21	0.952908061945	0.0986370544803	0.396853457909	1.99265026025	0.37886422228	1.27482402346	0.0377170515715	0.0897886810756	-0.157219546789	-0.205523609278	0.0349829359691	-0.0907215245401	0.138501831696	0.00964164824836	-0.0718670513598	0.141621986983	0.00334787014218	-0.0178086284257	0.199203868003	-0.0136868920037	0.208833766199	-11.4085774605	-1.87759145063	-92.1768917742

Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

ID	Refine TLS-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	chain 'A' and (resid 26 through 113 )	A	A	26 - 113	1 - 89
2	2	chain 'A' and (resid 114 through 150 )	A	A	114 - 150	90 - 126
3	3	chain 'A' and (resid 151 through 178 )	A	A	151 - 178	127 - 154
4	4	chain 'A' and (resid 179 through 199 )	A	A	179 - 199	155 - 175
5	5	chain 'A' and (resid 200 through 233 )	A	A	200 - 233	176 - 209
6	6	chain 'A' and (resid 234 through 296 )	A	A	234 - 296	210 - 272
7	7	chain 'A' and (resid 297 through 317 )	A	A	297 - 317	273 - 293
8	8	chain 'A' and (resid 318 through 361 )	A	A	318 - 361	294 - 337
9	9	chain 'B' and (resid 364 through 446 )	B	D	364 - 446	1 - 84
10	10	chain 'B' and (resid 447 through 530 )	B	D	447 - 530	85 - 168
11	11	chain 'B' and (resid 531 through 557 )	B	D	531 - 557	169 - 195
12	12	chain 'C' and (resid 26 through 52 )	C	I	26 - 52	1 - 27
13	13	chain 'C' and (resid 53 through 113 )	C	I	53 - 113	28 - 89
14	14	chain 'C' and (resid 114 through 146 )	C	I	114 - 146	90 - 122
15	15	chain 'C' and (resid 147 through 219 )	C	I	147 - 219	123 - 195
16	16	chain 'C' and (resid 220 through 270 )	C	I	220 - 270	196 - 246
17	17	chain 'C' and (resid 271 through 296 )	C	I	271 - 296	247 - 272
18	18	chain 'C' and (resid 297 through 317 )	C	I	297 - 317	273 - 293
19	19	chain 'C' and (resid 318 through 361 )	C	I	318 - 361	294 - 337
20	20	chain 'D' and (resid 364 through 431 )	D	L	364 - 431	1 - 69
21	21	chain 'D' and (resid 432 through 557 )	D	L	432 - 557	70 - 196

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