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Open data
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Basic information
Entry | Database: PDB / ID: 1cby | ||||||
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Title | DELTA-ENDOTOXIN | ||||||
![]() | DELTA-ENDOTOXIN CYTB | ||||||
![]() | TOXIN / MOSQUITOCIDAL / MEMBRANE PORE / CYTOLYTIC | ||||||
Function / homology | ![]() sporulation resulting in formation of a cellular spore / : / toxin activity / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Li, J. | ||||||
![]() | ![]() Title: Structure of the mosquitocidal delta-endotoxin CytB from Bacillus thuringiensis sp. kyushuensis and implications for membrane pore formation. Authors: Li, J. / Koni, P.A. / Ellar, D.J. #1: ![]() Title: Crystallization of a Membrane Pore-Forming Protein with Mosquitocidal Activity from Bacillus Thuringiensis Subsp. Kyushuensis Authors: Li, J. / Koni, P.A. / Ellar, D.J. #2: ![]() Title: Biochemical Characterization of Bacillus Thuringiensis Cytolytic Delta-Endotoxins Authors: Koni, P.A. / Ellar, D.J. #3: ![]() Title: Cloning and Characterization of a Novel Bacillus Thuringiensis Cytolytic Delta-Endotoxin Authors: Koni, P.A. / Ellar, D.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 57.2 KB | Display | ![]() |
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PDB format | ![]() | 42.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 29260.270 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Species: Bacillus thuringiensis / Strain: KYUSHUENSIS / Gene: CYTB / Plasmid: PCYTB / Gene (production host): CYTB / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.88 Å3/Da / Density % sol: 33 % | |||||||||||||||
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Crystal grow | *PLUS pH: 8.5 / Method: microdialysis | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Oct 25, 1993 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.87 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→19 Å / Num. obs: 7235 / % possible obs: 96.2 % / Observed criterion σ(I): 0 / Redundancy: 5.8 % / Rmerge(I) obs: 0.071 |
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Processing
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Refinement | Resolution: 2.6→19 Å / σ(F): 0
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Displacement parameters | Biso mean: 32 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.23 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→19 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 2.6 Å / Lowest resolution: 2.78 Å / Rfactor Rfree: 0.347 / Rfactor obs: 0.304 |