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Open data
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Basic information
Entry | Database: PDB / ID: 7czc | ||||||
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Title | Crystal structure of apo-FabG from Vibrio harveyi | ||||||
![]() | 3-oxoacyl-ACP reductase FabG | ||||||
![]() | OXIDOREDUCTASE / 3-oxo-acyl-ACP reductase / Rossmann fold / SDR-family / fatty acid metabolism | ||||||
Function / homology | 3-oxoacyl-(acyl-carrier-protein) reductase, putative / Short-chain dehydrogenase/reductase SDR / oxidoreductase activity / NAD(P)-binding domain superfamily / DI(HYDROXYETHYL)ETHER / 3-oxoacyl-ACP reductase FabG![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Singh, B.K. / Kumar, A. / Paul, B. / Biswas, R. / Das, A.K. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of apo-FabG from Vibrio harveyi Authors: Singh, B.K. / Kumar, A. / Paul, B. / Biswas, R. / Das, A.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 341.3 KB | Display | ![]() |
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PDB format | ![]() | 280.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 966 KB | Display | ![]() |
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Full document | ![]() | 962 KB | Display | |
Data in XML | ![]() | 36.8 KB | Display | |
Data in CIF | ![]() | 52.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1i01S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25978.738 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.76 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.2 / Details: 0.15M DL-malic acid (pH 7.2) , 20% w/v PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Feb 24, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→19.7504 Å / Num. obs: 65055 / % possible obs: 99.83 % / Redundancy: 2 % / CC1/2: 0.999 / Net I/σ(I): 21.02 |
Reflection shell | Resolution: 2→2.071 Å / Num. unique obs: 6451 / CC1/2: 0.858 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1I01 Resolution: 2→19.749 Å / SU ML: 0.22 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 23.98 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 114.66 Å2 / Biso mean: 31.6565 Å2 / Biso min: 15.45 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2→19.749 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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