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- PDB-7cr5: Complex structure of a human monoclonal antibody with SARS-CoV-2 ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7cr5 | ||||||
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Title | Complex structure of a human monoclonal antibody with SARS-CoV-2 nucleocapsid protein NTD | ||||||
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![]() | VIRAL PROTEIN/IMMUNE SYSTEM / Human monoclonal antibody / SARS-CoV-2 / nucleocapsid protein / Complex / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex | ||||||
Function / homology | ![]() cytoplasmic capsid assembly / viral RNA genome packaging / response to host immune response / negative regulation of interferon-beta production / Maturation of nucleoprotein / intracellular non-membrane-bounded organelle / positive regulation of NLRP3 inflammasome complex assembly / MHC class I protein binding / CD28 dependent PI3K/Akt signaling / SARS-CoV-2 targets host intracellular signalling and regulatory pathways ...cytoplasmic capsid assembly / viral RNA genome packaging / response to host immune response / negative regulation of interferon-beta production / Maturation of nucleoprotein / intracellular non-membrane-bounded organelle / positive regulation of NLRP3 inflammasome complex assembly / MHC class I protein binding / CD28 dependent PI3K/Akt signaling / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / protein sequestering activity / VEGFR2 mediated vascular permeability / molecular condensate scaffold activity / TAK1-dependent IKK and NF-kappa-B activation / NOD1/2 Signaling Pathway / DDX58/IFIH1-mediated induction of interferon-alpha/beta / MHC class I protein complex / Interleukin-1 signaling / RNA stem-loop binding / viral capsid / Interferon alpha/beta signaling / PIP3 activates AKT signaling / Transcription of SARS-CoV-2 sgRNAs / host cell endoplasmic reticulum-Golgi intermediate compartment / host cell Golgi apparatus / viral nucleocapsid / Translation of Structural Proteins / Virion Assembly and Release / host extracellular space / Induction of Cell-Cell Fusion / Attachment and Entry / host cell perinuclear region of cytoplasm / ribonucleoprotein complex / SARS-CoV-2 activates/modulates innate and adaptive immune responses / protein homodimerization activity / RNA binding / extracellular region / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chen, S. / Kang, S. | ||||||
Funding support | ![]()
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![]() | ![]() Title: A SARS-CoV-2 antibody curbs viral nucleocapsid protein-induced complement hyperactivation. Authors: Kang, S. / Yang, M. / He, S. / Wang, Y. / Chen, X. / Chen, Y.Q. / Hong, Z. / Liu, J. / Jiang, G. / Chen, Q. / Zhou, Z. / Zhou, Z. / Huang, Z. / Huang, X. / He, H. / Zheng, W. / Liao, H.X. / ...Authors: Kang, S. / Yang, M. / He, S. / Wang, Y. / Chen, X. / Chen, Y.Q. / Hong, Z. / Liu, J. / Jiang, G. / Chen, Q. / Zhou, Z. / Zhou, Z. / Huang, Z. / Huang, X. / He, H. / Zheng, W. / Liao, H.X. / Xiao, F. / Shan, H. / Chen, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 151.8 KB | Display | ![]() |
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PDB format | ![]() | 94.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 14750.459 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() |
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#2: Antibody | Mass: 24122.855 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#3: Antibody | Mass: 23099.518 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#4: Chemical | ChemComp-ZN / |
#5: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.83 Å3/Da / Density % sol: 56.52 % |
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Crystal grow | Temperature: 289.15 K / Method: vapor diffusion / pH: 7.2 Details: 0.01M Calcium chloride dihydrate, 0.05 M Sodium cacodylate trihydrate (pH 7.2) ,1.675M Ammonium sulfate, 0.5mM spermine |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 16, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.08→50 Å / Num. obs: 41759 / % possible obs: 99.9 % / Redundancy: 12.1 % / Biso Wilson estimate: 33.33 Å2 / CC1/2: 0.979 / CC star: 0.995 / Rmerge(I) obs: 0.22 / Rpim(I) all: 0.064 / Rrim(I) all: 0.23 / Χ2: 1.215 / Net I/av σ(I): 16.7 / Net I/σ(I): 13.18 |
Reflection shell | Resolution: 2.1→2.14 Å / Redundancy: 8.4 % / Rmerge(I) obs: 1.331 / Mean I/σ(I) obs: 1.64 / Num. unique obs: 2023 / CC1/2: 0.782 / CC star: 0.937 / Rrim(I) all: 1.405 / Χ2: 0.449 / % possible all: 99.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6M3M,6TCN Resolution: 2.08→28.58 Å / SU ML: 0.2646 / Cross valid method: FREE R-VALUE / σ(F): 1.37 / Phase error: 25.0681 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35.6 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.08→28.58 Å
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Refine LS restraints |
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LS refinement shell |
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