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- PDB-7cp0: Crystal Structure of double mutant Y115E Y117E human Secretory Gl... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7cp0 | ||||||
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Title | Crystal Structure of double mutant Y115E Y117E human Secretory Glutaminyl Cyclase | ||||||
![]() | Glutaminyl-peptide cyclotransferase | ||||||
![]() | TRANSFERASE / Glutaminyl-peptide cyclotransferase / sQC / Alzheimer's disease / Pyroglutamate | ||||||
Function / homology | ![]() peptidyl-pyroglutamic acid biosynthetic process, using glutaminyl-peptide cyclotransferase / glutaminyl-peptide cyclotransferase / glutaminyl-peptide cyclotransferase activity / protein modification process / specific granule lumen / tertiary granule lumen / ficolin-1-rich granule lumen / Neutrophil degranulation / zinc ion binding / extracellular exosome / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Dileep, K.V. / Ihara, K. / Sakai, N. / Shirozu, M. / Zhang, K.Y.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Piperidine-4-carboxamide as a new scaffold for designing secretory glutaminyl cyclase inhibitors. Authors: Dileep, K.V. / Sakai, N. / Ihara, K. / Kato-Murayama, M. / Nakata, A. / Ito, A. / Sivaraman, D.M. / Shin, J.W. / Yoshida, M. / Shirouzu, M. / Zhang, K.Y.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 220 KB | Display | ![]() |
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PDB format | ![]() | 175.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 467.7 KB | Display | ![]() |
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Full document | ![]() | 471 KB | Display | |
Data in XML | ![]() | 40.2 KB | Display | |
Data in CIF | ![]() | 59.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7cozC ![]() 4ywyS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 37331.121 Da / Num. of mol.: 3 / Mutation: Y115E, Y117E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() References: UniProt: Q16769, glutaminyl-peptide cyclotransferase #2: Chemical | #3: Chemical | #4: Chemical | ChemComp-EDO / #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.75 Å3/Da / Density % sol: 55.23 % / Description: Hexagonal plate like crystals |
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Crystal grow | Temperature: 277.15 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 100 mM MES buffer pH 6.5, 46-67 mM NaOH, 100 mM ammonium sulfate PH range: 6.0-6.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||
Detector | Type: RAYONIX MX-225 / Detector: CCD / Date: Jul 21, 2017 | ||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | ||||||||||||||||||||||||
Reflection | Resolution: 1.7→43.05 Å / Num. obs: 131782 / % possible obs: 99.8 % / Redundancy: 3.8 % / CC1/2: 0.996 / Rmerge(I) obs: 0.09 / Net I/σ(I): 10.6 / Num. measured all: 494444 / Scaling rejects: 151 | ||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4YWY Resolution: 1.7→43.05 Å / Cor.coef. Fo:Fc: 0.941 / Cor.coef. Fo:Fc free: 0.931 / SU B: 0.001 / SU ML: 0 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.093 / ESU R Free: 0.101 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||
Displacement parameters | Biso max: 61.93 Å2 / Biso mean: 17.651 Å2 / Biso min: 6.83 Å2
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Refinement step | Cycle: final / Resolution: 1.7→43.05 Å
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LS refinement shell | Resolution: 1.7→1.744 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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