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- PDB-7b0r: In meso structure of the membrane integral lipoprotein intramolec... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7b0r | ||||||
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Title | In meso structure of the membrane integral lipoprotein intramolecular transacylase Lit from Bacillus cereus with H85R mutation | ||||||
![]() | Hypothetical Membrane Spanning Protein | ||||||
![]() | MEMBRANE PROTEIN / lipoprotein / lipid cubic phase / Lit / transacylase | ||||||
Function / homology | Integral membrane protein 1906 / Protein of unknown function (DUF1461) / membrane / CITRIC ACID / (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate / 1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE / Hypothetical Membrane Spanning Protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Huang, C.-Y. / Olatunji, S. / Olieric, V. / Caffrey, M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis of the membrane intramolecular transacylase reaction responsible for lyso-form lipoprotein synthesis. Authors: Olatunji, S. / Bowen, K. / Huang, C.Y. / Weichert, D. / Singh, W. / Tikhonova, I.G. / Scanlan, E.M. / Olieric, V. / Caffrey, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 67.1 KB | Display | ![]() |
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PDB format | ![]() | 47.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7b0oSC ![]() 7b0pC ![]() 7b0qC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 27659.844 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 Gene: BC_1526 / Production host: ![]() ![]() |
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-Non-polymers , 6 types, 53 molecules ![](data/chem/img/GOL.gif)
![](data/chem/img/OLC.gif)
![](data/chem/img/PE5.gif)
![](data/chem/img/CIT.gif)
![](data/chem/img/PG5.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/OLC.gif)
![](data/chem/img/PE5.gif)
![](data/chem/img/CIT.gif)
![](data/chem/img/PG5.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | #3: Chemical | ChemComp-OLC / ( #4: Chemical | ChemComp-PE5 / | #5: Chemical | ChemComp-CIT / | #6: Chemical | ChemComp-PG5 / | #7: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.77 Å3/Da / Density % sol: 55.53 % |
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Crystal grow | Temperature: 293 K / Method: lipidic cubic phase Details: 100 mM MES/NaOH, pH 6.0, 125-200 mM M lithium citrate tribasic tetrahydrate, and 36-44 %(v/v) PEG 400 PH range: 6 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Apr 15, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.96862 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→40.8 Å / Num. obs: 14531 / % possible obs: 92.6 % / Redundancy: 6.48 % / CC1/2: 0.99 / Rrim(I) all: 0.15 / Net I/σ(I): 10.46 |
Reflection shell | Resolution: 2.2→2.33 Å / Mean I/σ(I) obs: 1.23 / Num. unique obs: 1674 / CC1/2: 0.64 / Rrim(I) all: 1.42 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 7B0O Resolution: 2.2→40.8 Å / Cross valid method: THROUGHOUT
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Displacement parameters | Biso max: 124.59 Å2 / Biso mean: 55.0676 Å2 / Biso min: 23.29 Å2 | ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→40.8 Å
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