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- PDB-7azc: DNA polymerase sliding clamp from Escherichia coli with peptide 2... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7azc | |||||||||
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Title | DNA polymerase sliding clamp from Escherichia coli with peptide 22 bound | |||||||||
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![]() | DNA BINDING PROTEIN / antibacterial drug | |||||||||
Function / homology | : ![]() | |||||||||
Biological species | ![]() ![]() synthetic construct (others) | |||||||||
Method | ![]() ![]() ![]() ![]() | |||||||||
![]() | Monsarrat, C. / Compain, G. / Andre, C. / Martiel, I. / Engilberge, S. / Olieric, V. / Wolff, P. / Brillet, K. / Landolfo, M. / Silva da Veiga, C. ...Monsarrat, C. / Compain, G. / Andre, C. / Martiel, I. / Engilberge, S. / Olieric, V. / Wolff, P. / Brillet, K. / Landolfo, M. / Silva da Veiga, C. / Wagner, J. / Guichard, G. / Burnouf, D.Y. | |||||||||
![]() | ![]() Title: Iterative Structure-Based Optimization of Short Peptides Targeting the Bacterial Sliding Clamp. Authors: Monsarrat, C. / Compain, G. / Andre, C. / Engilberge, S. / Martiel, I. / Olieric, V. / Wolff, P. / Brillet, K. / Landolfo, M. / Silva da Veiga, C. / Wagner, J. / Guichard, G. / Burnouf, D.Y. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 609.4 KB | Display | ![]() |
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PDB format | ![]() | 499 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7az5C ![]() 7az6C ![]() 7az7C ![]() 7az8C ![]() 7azdC ![]() 7azeC ![]() 7azfC ![]() 7azgC ![]() 7azkC ![]() 7azlC ![]() 6fvlS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 42801.863 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: dnaN, AD31_4438 / Production host: ![]() ![]() #2: Protein/peptide | Mass: 807.933 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 45.43 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion Details: 2% v/v Tacsimate pH 4.0, 0.1M Sodium acetate trihydrate pH 4.6, PEG 3350 16% (w/v) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Nov 1, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.77→72.02 Å / Num. obs: 107545 / % possible obs: 90.1 % / Redundancy: 6.4 % / Biso Wilson estimate: 24.09 Å2 / CC1/2: 0.998 / Rpim(I) all: 0.044 / Net I/σ(I): 12.1 |
Reflection shell | Resolution: 1.78→1.93 Å / Num. unique obs: 5173 / CC1/2: 0.48 / Rpim(I) all: 0.55 |
-Phasing
Phasing | Method: ![]() |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6FVL Resolution: 1.77→25.59 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.927 / SU R Cruickshank DPI: 0.168 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.181 / SU Rfree Blow DPI: 0.156 / SU Rfree Cruickshank DPI: 0.152
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Displacement parameters | Biso max: 141.79 Å2 / Biso mean: 29.53 Å2 / Biso min: 3 Å2
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Refine analyze | Luzzati coordinate error obs: 0.24 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.77→25.59 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.77→1.85 Å / Rfactor Rfree error: 0 / Total num. of bins used: 50
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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