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Open data
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Basic information
Entry | Database: PDB / ID: 7aah | ||||||
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Title | Structure of human pERp1 | ||||||
![]() | Marginal zone B- and B1-cell-specific protein | ||||||
![]() | IMMUNE SYSTEM / Folding factor / Endoplasmatic Reticulum / IgM | ||||||
Function / homology | ![]() regulation of B cell proliferation / regulation of insulin receptor signaling pathway / endoplasmic reticulum chaperone complex / integrin activation / positive regulation of immunoglobulin production / regulation of cell population proliferation / endoplasmic reticulum lumen / apoptotic process / positive regulation of cell population proliferation / extracellular region / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Sowa, S.T. / Moilanen, A. / Biterova, E. / Saaranen, M.J. / Lehtio, L. / Ruddock, L.W. | ||||||
Funding support | ![]()
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![]() | ![]() Title: High-resolution Crystal Structure of Human pERp1, A Saposin-like Protein Involved in IgA, IgM and Integrin Maturation in the Endoplasmic Reticulum. Authors: Sowa, S.T. / Moilanen, A. / Biterova, E. / Saaranen, M.J. / Lehtio, L. / Ruddock, L.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 188.8 KB | Display | ![]() |
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PDB format | ![]() | 159.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.3 KB | Display | ![]() |
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Full document | ![]() | 453.4 KB | Display | |
Data in XML | ![]() | 16.5 KB | Display | |
Data in CIF | ![]() | 24.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18257.389 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-CIT / | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 38.63 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop Details: Lithium citrate tribasic tetrahydrate, Glycerol ethoxylate, n-decyl-beta-D-maltopyranoside |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 6, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.96863 Å / Relative weight: 1 |
Reflection | Resolution: 1.4→47.8 Å / Num. obs: 58099 / % possible obs: 99.1 % / Redundancy: 6.2 % / CC1/2: 0.999 / Net I/σ(I): 12.88 |
Reflection shell | Resolution: 1.4→1.45 Å / Num. unique obs: 5426 / CC1/2: 0.364 |
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Processing
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Refinement | Method to determine structure: AB INITIO PHASING / Resolution: 1.4→47.764 Å / SU ML: 0.18 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 20.32 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.4→47.764 Å
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Refine LS restraints |
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LS refinement shell |
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