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- PDB-6k8q: Solution structure of the intermembrane space domain of the mitoc... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6k8q | |||||||||
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Title | Solution structure of the intermembrane space domain of the mitochondrial import protein Tim21 from S. cerevisiae | |||||||||
![]() | Mitochondrial import inner membrane translocase subunit TIM21 | |||||||||
![]() | PROTEIN TRANSPORT / membrane / mitochondria / translocation | |||||||||
Function / homology | ![]() TIM23 mitochondrial import inner membrane translocase complex / protein import into mitochondrial matrix / protein insertion into mitochondrial inner membrane / mitochondrial inner membrane / mitochondrion Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | SOLUTION NMR / simulated annealing | |||||||||
![]() | Bala, S. / Shinya, S. / Srivastava, A. / Shimada, A. / Kobayashi, N. / Kojima, C. / Tama, F. / Miyashita, O. / Kohda, D. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal contact-free conformation of an intrinsically flexible loop in protein crystal: Tim21 as the case study. Authors: Bala, S. / Shinya, S. / Srivastava, A. / Ishikawa, M. / Shimada, A. / Kobayashi, N. / Kojima, C. / Tama, F. / Miyashita, O. / Kohda, D. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 857.1 KB | Display | ![]() |
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PDB format | ![]() | 728 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 534.6 KB | Display | ![]() |
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Full document | ![]() | 736.9 KB | Display | |
Data in XML | ![]() | 59.7 KB | Display | |
Data in CIF | ![]() | 78.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6k7dC ![]() 6k7eC ![]() 6k7fC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 13571.599 Da / Num. of mol.: 1 / Fragment: intermembrane space domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: TIM21, YGR033C / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 323 uM [U-13C; U-15N] Tim21, 20 mM TRIS, 50 mM sodium chloride, 90% H2O/10% D2O Label: 13C15N_sample / Solvent system: 90% H2O/10% D2O | ||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 50 mM / Label: conditions_1 / pH: 6.5 / Pressure: 1 atm / Temperature: 300 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |