PDB-7a0u: LIGAND FREE TYPE II E. COLI ASPARAGINASE T12S/T89S DOUBLE MUTANT

ID or keywords:

Entry

Database: PDB / ID: 7a0u

Title

LIGAND FREE TYPE II E. COLI ASPARAGINASE T12S/T89S DOUBLE MUTANT

Components

L-asparaginase 2

Asparaginase

Keywords

ANTITUMOR PROTEIN / LIGAND-FREE /

ACUTE LYMPHOBLASTIC LEUKEMIA / 3.5.1.1 /

ASPARAGINASE /

AMIDOHYDROLASE

Function / homology

Function and homology information

asparagine catabolic process /

asparaginase /

asparaginase activity / outer membrane-bounded periplasmic space / protein homotetramerization /

periplasmic space / protein-containing complex / identical protein binding
Similarity search - Function

Biological species

Escherichia coli (E. coli)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT /

molecular replacement / Resolution: 2.26048715056 Å

Authors

Maggi, M. / Scotti, C.

Citation

Journal: To Be Published
Title: LIGAND FREE TYPE II E. COLI ASPARAGINASE T12S/T89S DOUBLE MUTANT
Authors: Maggi, M. / Scotti, C.

History

Deposition	Aug 10, 2020	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Aug 18, 2021	Provider: repository / Type: Initial release
Revision 1.1	Jan 31, 2024	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

Structure viewer	Molecule: MolmilJmol/JSmol

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PDBx/mmCIF format	7a0u.cif.gz	936.8 KB	Display	PDBx/mmCIF format
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PDBx/mmJSON format	7a0u.json.gz		Tree view	PDBx/mmJSON format
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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/a0/7a0u ftp://data.pdbj.org/pub/pdb/validation_reports/a0/7a0u	HTTPS FTP

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Related structure data

Related structure data	1nnsS 3ecaS S: Starting model for refinement
Similar structure data	6nx9-1 6yzi-2 6pab-1 6nx9-2 1jja-1 6rue-d 6nxa-1 7a0u-2 6pab-2 6pa9-1 Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

A: L-asparaginase 2

B: L-asparaginase 2

C: L-asparaginase 2

D: L-asparaginase 2

142 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: L-asparaginase 2

C: L-asparaginase 2

A: L-asparaginase 2

C: L-asparaginase 2

defined by author&software
Evidence: gel filtration
142 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: L-asparaginase 2

D: L-asparaginase 2

defined by author&software
142 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	150.850, 61.860, 142.420
Angle α, β, γ (deg.)	90.000, 117.935, 90.000
Int Tables number	5
Space group name H-M	C121
Space group name Hall	C2y

Components on special symmetry positions

ID	Model	Components
1	1	A-524- HOH
2	1	A-577- HOH
3	1	B-529- HOH
4	1	C-497- HOH
5	1	D-504- HOH

#1: Protein	L-asparaginase 2 / Asparaginase / L-asparaginase II / L-ASNase II / L-asparagine amidohydrolase II Mass: 35558.824 Da / Num. of mol.: 4 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (strain K12) (bacteria) Strain: K12 / Gene: ansB, b2957, JW2924 / Plasmid: PET101/D-TOPO / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): DELTA ansA and ansB / References: UniProt: P00805, asparaginase
#2: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 680 / Source method: isolated from a natural source / Formula: H₂O

#1: Protein

L-asparaginase 2 /

Asparaginase / L-asparaginase II / L-ASNase II / L-asparagine amidohydrolase II

Mass: 35558.824 Da / Num. of mol.: 4 / Mutation: YES
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Escherichia coli (strain K12) (bacteria)
Strain: K12 / Gene: ansB, b2957, JW2924 / Plasmid: PET101/D-TOPO / Production host:

Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): DELTA ansA and ansB / References: UniProt: P00805,

asparaginase

#2: Water

ChemComp-HOH / water /

Water

Mass: 18.015 Da / Num. of mol.: 680 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.13 Å³/Da / Density % sol: 42.33 %
Crystal grow	Temperature: 290.15 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 0.1 M HEPES, 10% PEG 8000, 8% ETHYLENE GLYCOL, PH 7.5

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Data collection

Diffraction

Mean temperature: 80 K / Serial crystal experiment: N

Diffraction source

Source:

SYNCHROTRON / Site:

ESRF

/ Beamline: ID23-2 / Wavelength: 0.899998 Å

Detector

Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 26, 2016 / Details: TOROIDAL MIRROR

Radiation

Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.899998 Å / Relative weight: 1

Reflection

Resolution: 2.26→125.83 Å / Num. obs: 53012 / % possible obs: 96.8 % / Redundancy: 2.861 % / B_iso Wilson estimate: 26.1577580225 Å² / CC_1/2: 0.981 / R_merge(I) obs: 0.138 / R_rim(I) all: 0.17 / Χ²: 0.967 / Net I/σ(I): 5.64 / Num. measured all: 151671 / Scaling rejects: 11

Reflection shell

Diffraction-ID: 1

Resolution (Å)	Redundancy (%)	R_merge(I) obs	Mean I/σ(I) obs	Num. measured obs	Num. possible	Num. unique obs	CC_1/2	R_rim(I) all	% possible all
2.26-2.32	2.906	0.415	2.35	11559	4020	3977	0.686	0.508	98.9
2.32-2.38	2.876	0.353	2.8	11081	3903	3853	0.709	0.433	98.7
2.38-2.45	2.843	0.315	3.07	10586	3800	3724	0.788	0.387	98
2.45-2.53	2.698	0.297	3.17	9697	3703	3594	0.8	0.367	97.1
2.53-2.61	2.889	0.264	3.7	10217	3600	3536	0.848	0.323	98.2
2.61-2.7	2.937	0.248	4.06	10121	3496	3446	0.874	0.303	98.6
2.7-2.8	2.927	0.215	4.65	9668	3356	3303	0.904	0.263	98.4
2.8-2.92	2.882	0.192	5.22	9150	3241	3175	0.92	0.235	98
2.92-3.05	2.782	0.168	5.66	8340	3077	2998	0.942	0.207	97.4
3.05-3.2	2.798	0.148	6.38	8021	2961	2867	0.957	0.182	96.8
3.2-3.37	2.929	0.135	7.16	8177	2851	2792	0.964	0.165	97.9
3.37-3.57	2.928	0.128	7.55	7596	2660	2594	0.967	0.157	97.5
3.57-3.82	2.809	0.119	7.81	6074	2553	2162	0.969	0.147	84.7
3.82-4.13	2.698	0.104	8.37	5914	2341	2192	0.977	0.129	93.6
4.13-4.52	2.972	0.096	9.72	6229	2162	2096	0.977	0.118	96.9
4.52-5.05	2.941	0.092	9.98	5618	1975	1910	0.981	0.112	96.7
5.05-5.84	2.814	0.096	9.07	4674	1752	1661	0.977	0.118	94.8
5.84-7.15	2.858	0.091	9.56	4024	1475	1408	0.977	0.112	95.5
7.15-10.11	2.906	0.076	10.93	3231	1174	1112	0.987	0.093	94.7
10.11-125.83	2.768	0.076	11.11	1694	670	612	0.989	0.095	91.3

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Phasing

Phasing

Method:

molecular replacement

Phasing MR

	Highest resolution	Lowest resolution
Rotation	3.43 Å	125.83 Å
Translation	3.43 Å	125.83 Å

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Processing

Software

Name	Version	Classification
PHENIX	1.11.1_2575	refinement
PHENIX	1.11.1_2575	refinement
XDS		data reduction
XSCALE		data scaling
PHASER	2.8.3	phasing
PDB_EXTRACT	3.25	data extraction

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 3ECA, 1NNS

3eca

1nns

Resolution: 2.26048715056→125.82516793 Å / SU ML: 0.283222669525 / Cross valid method: THROUGHOUT / σ(F): 1.34654533639 / Phase error: 23.7196940376
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.239891031969	2659	5.01792791093 %
R_work	0.171340308687	50331	-
obs	0.17478859218	52990	96.9004297339 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 32.0203616284 Å²

Refinement step

Cycle: LAST / Resolution: 2.26048715056→125.82516793 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	9367	0	0	680	10047

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0126943150789	9516
X-RAY DIFFRACTION	f_angle_d	1.14547842139	12966
X-RAY DIFFRACTION	f_chiral_restr	0.0605229197282	1553
X-RAY DIFFRACTION	f_plane_restr	0.00696697716428	1695
X-RAY DIFFRACTION	f_dihedral_angle_d	12.2731222613	5734

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.2605-2.3016	0.288186884814	150	0.252816425325	2686	X-RAY DIFFRACTION	98.9532449407
2.3016-2.3459	0.324086130491	124	0.223869322256	2675	X-RAY DIFFRACTION	98.8696573649
2.3459-2.3938	0.302176724213	143	0.224022388986	2686	X-RAY DIFFRACTION	98.5714285714
2.3938-2.4458	0.279648128067	155	0.21018524375	2644	X-RAY DIFFRACTION	98.1072555205
2.4458-2.5027	0.263465368654	140	0.204287647652	2646	X-RAY DIFFRACTION	97.6858345021
2.5027-2.5653	0.279878900704	149	0.192451464612	2633	X-RAY DIFFRACTION	97.0690858339
2.5653-2.6347	0.288073205646	140	0.186851821868	2709	X-RAY DIFFRACTION	99.0611961057
2.6347-2.7122	0.27157639942	133	0.191459852892	2675	X-RAY DIFFRACTION	98.4917572781
2.7122-2.7997	0.256228424581	136	0.183069792417	2674	X-RAY DIFFRACTION	98.5619081024
2.7997-2.8998	0.265010859682	152	0.191301585326	2668	X-RAY DIFFRACTION	98.3263598326
2.8998-3.0159	0.27581135893	150	0.171798750691	2653	X-RAY DIFFRACTION	97.6655052265
3.0159-3.1532	0.257706109894	145	0.174392278499	2632	X-RAY DIFFRACTION	96.1898164184
3.1532-3.3195	0.213939180002	153	0.170745558726	2653	X-RAY DIFFRACTION	98.2837127846
3.3195-3.5274	0.262956135035	132	0.162409431019	2676	X-RAY DIFFRACTION	97.9420997558
3.5274-3.7998	0.206973203008	99	0.160023196244	2389	X-RAY DIFFRACTION	86.2690707351
3.7998-4.1822	0.195508585496	130	0.146085774354	2609	X-RAY DIFFRACTION	94.4482758621
4.1822-4.7874	0.191285414362	162	0.124237518549	2685	X-RAY DIFFRACTION	97.3000683527
4.7874-6.0317	0.225005621178	131	0.146028272334	2634	X-RAY DIFFRACTION	95.3119613926
6.0317-125	0.195699126903	135	0.167038350688	2704	X-RAY DIFFRACTION	94.3816489362

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	3.45862250676	0.389532969826	0.275537874043	1.84476889809	0.181341436408	3.31404463766	-0.26714893165	0.369654638427	0.167579371833	-0.210507449741	0.221071122637	-0.123747840956	-0.251875455742	0.319870678456	0.0460920794977	0.402191802112	-0.022899031296	0.0365872685782	0.392854435848	-0.0304844711507	0.325580202589	54.3162643212	45.2330705724	33.6217147159
2	1.89389911342	0.279070466634	0.485645844634	0.820408686089	0.210010107502	1.13783070524	-0.0435650795174	0.0616121158187	-0.129182422702	-0.038814609177	0.0374872354422	0.00219505791679	-0.0246178772544	0.0492240051732	5.54456547317E-5	0.286758949797	0.0283658628166	0.0335430602654	0.260772916881	-0.00522567009575	0.194835298707	42.9300821644	41.6537275082	42.3187689326
3	1.00930488165	-0.417771181553	0.183707200543	0.7562717419	0.06978888096	0.406626543192	0.00366394391004	0.079394575769	-0.0764439075558	-0.0539885486516	-0.0330301979946	0.133836337868	0.0665772325013	-0.0486332646287	0.0282654058779	0.271982991436	0.0155997041751	0.00421549402012	0.234850026309	-0.0185463106857	0.184586621875	30.3033321029	46.2401662196	51.2738125308
4	2.99274873004	-0.238458621852	0.966646514171	1.79380034086	-0.387760340274	1.08563188428	0.11150923893	-0.165768821411	-0.0266340888576	0.14036254149	-0.102425267109	0.280171441615	0.119274652119	-0.255980398718	-0.00724766988491	0.30174126234	-0.0396884758065	0.0251801337358	0.305270601861	-0.048384817265	0.243006676793	18.7096098713	43.7108316772	55.539060775
5	1.57716205641	0.026451719177	-1.02091798239	2.77119291827	0.984536230849	1.09669203128	0.0915793334093	0.356850523458	0.196817986651	-0.78411176833	-0.368274040504	-0.138397946114	0.105217741017	0.220237370275	0.255804810856	0.281144449786	-0.0255720548426	-0.0533630609534	0.276707922729	0.0491890667815	0.363797428965	16.8698845122	44.5526013239	-21.6689854893
6	1.11260156527	-0.433932372711	0.241386554205	0.855549766718	-0.52661182909	0.618532601633	-0.0248296055707	0.345602346454	0.125148820618	-0.340662982558	0.0290933981406	-0.0506426848141	0.0587343582847	-0.0747328618963	-0.0279346030411	0.368025082652	-0.0376404992283	0.00969515233277	0.336310204893	0.0769625630569	0.247929228678	22.2407544815	39.5643132388	-16.9351136599
7	1.83304248051	-0.135974891883	1.09002018234	0.249551658155	0.359099463499	1.50889887009	-0.217484298534	0.301187959402	0.208663079824	0.0594453197819	-0.0516331264595	-0.0884034113582	-0.236769826286	0.226267666237	0.261592570235	0.164390171132	-0.0212025413185	0.0161552517308	0.249640329248	0.073788225548	0.275514721673	23.3571121188	45.6367365082	-8.66850289942
8	1.11961460016	0.764166689739	0.546644800007	0.619019398002	-0.00225858941452	1.70301748658	-0.0328359416476	0.05280792135	-0.0648619712247	-0.01906998448	-0.0356344620349	-0.353987147036	-0.0714810242655	0.00851685047685	0.0390385867275	0.201409670995	0.00965090629629	0.0352016095922	0.244620223522	0.0134103299329	0.248809052526	16.7189542794	38.3168380547	-8.75321702127
9	0.457731449149	-0.146354449249	-0.0452462974541	0.637278165987	-0.168957161573	0.490694686488	-0.0213857276337	0.0551092988901	0.066941268397	-0.00071432293575	-0.00684646413565	-0.0245302035766	-0.121022436664	0.00118262629723	0.0332431569553	0.164749644108	-0.0138404205468	0.00516642616938	0.206111055183	0.0117909569918	0.240626126859	14.1577418466	37.8763797691	0.451385506167
10	3.01617778453	0.338401440279	1.68215725439	0.993929418124	0.392234753348	2.41760334045	0.0851080238294	-0.337141542458	0.0749909993858	0.0442606371639	-0.0274859845645	-0.0184483227085	0.13472995072	-0.0441818197053	-0.0478846495456	0.235327801615	0.0100753972999	0.0207977159766	0.226537560962	-0.00958248330528	0.220227675172	17.192711357	33.8401999315	20.7088859313
11	0.898224194999	0.10479492188	0.148140401969	0.837489857811	-0.695258006607	1.6328827569	-0.0582081508005	-0.0264326902403	0.139352748027	0.0938952714201	-0.0260334340375	-0.210545382577	-0.158700013163	-0.0339184185248	0.0763709835921	0.214648086899	-0.0146034081684	-0.00302989600378	0.172930725897	-0.0312957369084	0.306799059951	17.992563566	43.197356203	14.4397695201
12	1.42211135561	0.665766570072	1.52029894092	2.2829345358	0.846761024359	1.87499856867	0.243398543686	-0.074229381871	0.0539739706402	0.545175465383	-0.110541362491	-0.213248450451	0.420731080667	-0.350031024275	-0.166340949336	0.447634546465	-0.0216733739925	0.022055328254	0.410701768312	-0.028190009761	0.38578414154	18.8529649864	10.2099096191	79.1414248664
13	0.438832312537	-0.0907584935231	0.112847127694	0.654173221417	0.303177958496	1.19457558973	-0.0485004138684	-0.0993005285199	0.100628711217	0.105243831569	0.00451941503331	0.105571862846	-0.178449374316	-0.112797054832	0.0453364796977	0.291689701005	0.0394411767874	0.0203123361348	0.232206868259	-0.0121394569978	0.237104194523	24.7791624751	8.16606124028	63.9401749798
14	2.29638869766	0.391504713565	1.35171076118	1.10785651707	0.14341011797	3.33605240199	-0.0957290307959	0.124748630255	0.114523333446	-0.229054479528	0.0648496019661	-0.0282045823434	-0.102686283687	-0.0503876983898	0.0358233171576	0.418172187165	0.0264522144532	0.0453200286126	0.229672450986	0.0479710481327	0.295718303205	28.7178733002	2.52926364032	39.543289173
15	0.554740451157	-0.0996569992055	-0.267336473164	1.12012961027	0.214971366327	1.67817606481	-0.136152663137	0.0984122812207	0.172248383327	-0.355958631277	-0.0822778427468	0.0971807673536	-0.389497885415	-0.162335929638	0.224345536479	0.39785233882	0.032763144202	-0.0498989238994	0.265183853866	0.00899164098354	0.29602130702	26.8320385963	12.0455321518	45.3124169073
16	2.46061614834	-1.5428655051	-1.05320090938	2.13972439717	0.658826826501	3.45187205525	0.191635875384	-0.688084753829	0.190394873613	0.3847415231	-0.198225577551	0.489323336231	-0.0798280769554	-0.0021446520028	-0.033359178968	0.337342051272	-0.0425211806953	0.0374447073863	0.447610734318	-0.0259753647453	0.306294500518	-5.42299682506	11.6984219491	31.4934801801
17	1.96270377174	-0.270603344722	0.0519575148564	0.797421115836	-0.0682418981615	0.5431090405	0.0295735916658	-0.240812927586	-0.165541027225	0.159047471548	-0.0016811581775	0.0629959658297	0.00773308978912	-0.104473683285	-0.0238939608038	0.247861012344	-0.0276882527304	-0.00960166894629	0.280391721533	0.0209667867579	0.199107589246	3.82667248113	11.0756544112	23.1534840106
18	2.28086480697	-0.21240726476	-0.558188806887	0.759292830785	0.535735204459	1.88547614881	0.0683196224412	-0.0161068413738	-0.110268256903	-0.0243660010793	0.00869215509748	0.0643554465897	-0.0848016989943	0.0415355756606	-0.121343267612	0.206418594481	-0.0190294590998	-0.0142282500225	0.227519186261	0.0264639822279	0.226646818652	-2.31975251283	11.450549332	14.0154002701
19	2.2754654046	0.0215428803198	-1.0341753056	1.77132673757	1.62861749162	4.15031970867	0.126199669636	0.149612518526	0.268863651924	-0.0302916445796	0.0675241505536	0.0984256457244	0.044779405479	0.0885324189766	-0.202847701739	0.249803951748	0.0385841374444	-0.0217312182546	0.219368931717	0.0511956215358	0.252508844828	2.28893500356	21.0865904313	8.07439633626
20	1.58737812628	0.268471364955	-0.0614330481962	0.982264749385	-0.180485532487	0.564638382559	0.0147064584173	-0.0568889241541	-0.0919768182458	0.0204884006306	-0.0377079249728	-0.116187234005	0.0722666264687	-0.0270413584583	0.0167539373579	0.189224762599	0.0164241105105	-0.0152552885127	0.181624931649	-0.00988115858328	0.192764719757	16.9604860431	14.0695732031	9.71885793227
21	2.77228701025	0.604045564796	-0.554700846712	1.86290098007	-0.232357568223	1.09746869778	-0.0440341605463	0.129121304023	-0.195227567241	-0.0200395093431	0.0515792653047	-0.246441569921	0.101709145112	0.116227261709	0.0118459066898	0.208639370799	0.0154915299727	-0.0224075286227	0.215070103417	-0.0227820091696	0.204160064609	24.4637322841	13.1820714939	3.02298881797

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'B' and (resid 0 through 44 )
2	X-RAY DIFFRACTION	2	chain 'B' and (resid 45 through 156 )
3	X-RAY DIFFRACTION	3	chain 'B' and (resid 157 through 250 )
4	X-RAY DIFFRACTION	4	chain 'B' and (resid 251 through 326 )
5	X-RAY DIFFRACTION	5	chain 'C' and (resid -3 through 39 )
6	X-RAY DIFFRACTION	6	chain 'C' and (resid 40 through 62 )
7	X-RAY DIFFRACTION	7	chain 'C' and (resid 63 through 91 )
8	X-RAY DIFFRACTION	8	chain 'C' and (resid 92 through 124 )
9	X-RAY DIFFRACTION	9	chain 'C' and (resid 125 through 250 )
10	X-RAY DIFFRACTION	10	chain 'C' and (resid 251 through 285 )
11	X-RAY DIFFRACTION	11	chain 'C' and (resid 286 through 326 )
12	X-RAY DIFFRACTION	12	chain 'D' and (resid 1 through 39 )
13	X-RAY DIFFRACTION	13	chain 'D' and (resid 40 through 250 )
14	X-RAY DIFFRACTION	14	chain 'D' and (resid 251 through 285 )
15	X-RAY DIFFRACTION	15	chain 'D' and (resid 286 through 326 )
16	X-RAY DIFFRACTION	16	chain 'A' and (resid 0 through 34 )
17	X-RAY DIFFRACTION	17	chain 'A' and (resid 35 through 124 )
18	X-RAY DIFFRACTION	18	chain 'A' and (resid 125 through 162 )
19	X-RAY DIFFRACTION	19	chain 'A' and (resid 163 through 179 )
20	X-RAY DIFFRACTION	20	chain 'A' and (resid 180 through 250 )
21	X-RAY DIFFRACTION	21	chain 'A' and (resid 251 through 326 )

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