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- PDB-6rue: Wolinella succinogenes L-asparaginase mutant V23Q,K24T with L-Asp -
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Open data
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Basic information
Entry | Database: PDB / ID: 6rue | ||||||
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Title | Wolinella succinogenes L-asparaginase mutant V23Q,K24T with L-Asp | ||||||
![]() | (L-asparaginase) x 4 | ||||||
![]() | HYDROLASE | ||||||
Function / homology | ![]() asparagine metabolic process / asparaginase / asparaginase activity / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Timofeev, V.I. / Kuranova, I.P. | ||||||
![]() | ![]() Title: Features of the Three Dimensional Structure of the Mutant Form of Wolinella succinogenes L-Asparaginase in Complexes with L-Aspartic and L-Glutamic Acids Authors: Timofeev, V.I. / Zhukhlistova, N.E. / Kuranova, I.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 262.4 KB | Display | ![]() |
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PDB format | ![]() | 204.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 468.8 KB | Display | ![]() |
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Full document | ![]() | 475.6 KB | Display | |
Data in XML | ![]() | 51.2 KB | Display | |
Data in CIF | ![]() | 75.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 4 types, 4 molecules ABCD
#1: Protein | Mass: 33688.434 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: ansA, ansB, WS0660 / Production host: ![]() ![]() |
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#2: Protein | Mass: 33402.148 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: ansA, ansB, WS0660 / Production host: ![]() ![]() |
#3: Protein | Mass: 33473.227 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: ansA, ansB, WS0660 / Production host: ![]() ![]() |
#4: Protein | Mass: 33617.355 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: ansA, ansB, WS0660 / Production host: ![]() ![]() |
-Non-polymers , 2 types, 774 molecules ![](data/chem/img/ASP.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | ChemComp-ASP / #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 48.16 % |
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Crystal grow | Temperature: 293 K / Method: counter-diffusion / Details: PEG |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Feb 13, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→40 Å / Num. obs: 146129 / % possible obs: 97.58 % / Redundancy: 2.89 % / Net I/σ(I): 5.4172 |
Reflection shell | Resolution: 1.65→1.74 Å / Num. unique obs: 21413 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 17.168 Å2
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Refinement step | Cycle: 1 / Resolution: 1.65→30 Å
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Refine LS restraints |
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