PDB-6ywo: CutA in complex with A3 RNA

ID or keywords:

Entry

Database: PDB / ID: 6ywo

Title

CutA in complex with A3 RNA

Components

CutA
RNA (5'-R(*AP*AP*A)-3')

Keywords

RNA BINDING PROTEIN / terminal nucleotide transferase /

polymerase /

RNA-binding protein

Function / homology

TUTase nucleotidyltransferase domain / PAP/25A-associated / Cid1 family poly A polymerase / poly(A) RNA polymerase activity /

Polymerase, nucleotidyl transferase domain /

Nucleotidyltransferase domain / Nucleotidyltransferase superfamily /

RNA / Uncharacterized protein

Function and homology information

Biological species

Thermothielavioides terrestris NRRL 8126 (fungus)
synthetic construct (others)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.9 Å

Authors

Malik, D. / Kobylecki, K. / Krawczyk, P. / Poznanski, J. / Jakielaszek, A. / Napiorkowska, A. / Dziembowski, A. / Tomecki, R. / Nowotny, M.

Funding support

Poland, 1items

Citation

Journal: Nucleic Acids Res. / Year: 2020
Title: Structure and mechanism of CutA, RNA nucleotidyl transferase with an unusual preference for cytosine.
Authors: Malik, D. / Kobylecki, K. / Krawczyk, P. / Poznanski, J. / Jakielaszek, A. / Napiorkowska, A. / Dziembowski, A. / Tomecki, R. / Nowotny, M.

History

Deposition	Apr 29, 2020	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Aug 5, 2020	Provider: repository / Type: Initial release
Revision 1.1	Sep 16, 2020	Group: Database references / Category: citation / citation_author Item: _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed ..._citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID
Revision 1.2	Sep 30, 2020	Group: Database references / Category: citation Item: _citation.journal_volume / _citation.page_first / _citation.page_last

Structure viewer	Molecule: MolmilJmol/JSmol

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PDBx/mmCIF format	6ywo.cif.gz	729.2 KB	Display	PDBx/mmCIF format
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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/yw/6ywo ftp://data.pdbj.org/pub/pdb/validation_reports/yw/6ywo	HTTPS FTP

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Related structure data

Related structure data	6ywnC 6ywpC C: citing same article (ref.)
Similar structure data	1n2k-d 4cia-d 4e7x-3 4djk-2 4mkx-d 6gde-d 5w0m-1 5c3r-1 4az3-d 4cib-d Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

Related structure data

6ywnC

6ywpC

C: citing same article (ref.)

Similar structure data

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#106 - Oct 2008 Poly(A) Polymerase similarity (2)

Deposited unit

A: CutA

B: CutA

C: CutA

D: CutA

E: RNA (5'-R(*AP*AP*A)-3')

I: RNA (5'-R(*AP*AP*A)-3')

F: RNA (5'-R(*AP*AP*A)-3')

K: RNA (5'-R(*AP*AP*A)-3')

hetero molecules

174 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: CutA

F: RNA (5'-R(*AP*AP*A)-3')

hetero molecules

defined by author&software
43.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: CutA

E: RNA (5'-R(*AP*AP*A)-3')

hetero molecules

defined by author&software
43.6 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

C: CutA

I: RNA (5'-R(*AP*AP*A)-3')

hetero molecules

defined by author&software
43.5 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

D: CutA

K: RNA (5'-R(*AP*AP*A)-3')

hetero molecules

defined by author&software
43.6 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	56.920, 56.950, 149.890
Angle α, β, γ (deg.)	79.859, 87.116, 75.832
Int Tables number	1
Space group name H-M	P1
Space group name Hall	P1
Symmetry operation	#1: x,y,z

#1: Protein	CutA Mass: 42521.148 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermothielavioides terrestris NRRL 8126 (fungus) Gene: THITE_2112714 / Plasmid: pet28SUMO Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) Variant (production host): RIL / References: UniProt: G2R014
#2: RNA chain	RNA (5'-R(APAPA)-3') Mass: 942.660 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source*: (synth.) synthetic construct (others)
#3: Chemical	ChemComp-CL / CHLORIDE ION / Chloride Mass: 35.453 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Cl
#4: Chemical	ChemComp-MG / MAGNESIUM ION Mass: 24.305 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Formula: Mg
#5: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 999 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.77 Å³/Da / Density % sol: 55.54 %
Crystal grow	Temperature: 298.15 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 0.1 M Bis-Tris pH 6.5, 0.2 M lithium sulfate monohydrate and 25% polyethylene glycol 3350

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P13 (MX1) / Wavelength: 0.979 Å
Detector	Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 25, 2017
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.979 Å / Relative weight: 1
Reflection	Resolution: 1.9→33.26 Å / Num. obs: 136433 / % possible obs: 96.1 % / Redundancy: 3.6 % / B_iso Wilson estimate: 32.71 Å² / CC_1/2: 0.99 / Net I/σ(I): 8.49
Reflection shell	Resolution: 1.9→2.01 Å / Redundancy: 3.6 % / Mean I/σ(I) obs: 1.2 / Num. unique obs: 21579 / CC_1/2: 0.59 / % possible all: 94.3

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Processing

Software

Name	Version	Classification
PHENIX	1.18.2_3874	refinement
PHENIX	1.18.2_3874	refinement
XDS		data reduction
XSCALE		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: XXXX

Resolution: 1.9→33.26 Å / SU ML: 0.2405 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 22.8665
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2126	6817	5 %
R_work	0.1758	129557	-
obs	0.1777	136374	96.12 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 41.11 Å²

Refinement step

Cycle: LAST / Resolution: 1.9→33.26 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	11133	252	14	999	12398

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0088	11799
X-RAY DIFFRACTION	f_angle_d	0.9453	16091
X-RAY DIFFRACTION	f_chiral_restr	0.0551	1829
X-RAY DIFFRACTION	f_plane_restr	0.0055	2040
X-RAY DIFFRACTION	f_dihedral_angle_d	22.7124	4437

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.9-1.92	0.3929	204	0.3565	3895	X-RAY DIFFRACTION	88.53
1.92-1.94	0.3303	230	0.2974	4364	X-RAY DIFFRACTION	95.61
1.94-1.97	0.35	229	0.283	4344	X-RAY DIFFRACTION	95.43
1.97-1.99	0.2762	222	0.2546	4226	X-RAY DIFFRACTION	95.8
1.99-2.02	0.2819	226	0.2394	4288	X-RAY DIFFRACTION	95.8
2.02-2.04	0.2638	230	0.2233	4370	X-RAY DIFFRACTION	95.67
2.04-2.07	0.2654	221	0.2227	4200	X-RAY DIFFRACTION	95.36
2.07-2.11	0.2726	230	0.2193	4366	X-RAY DIFFRACTION	95.45
2.11-2.14	0.2718	221	0.2157	4202	X-RAY DIFFRACTION	95.04
2.14-2.17	0.2517	228	0.2081	4326	X-RAY DIFFRACTION	95.77
2.17-2.21	0.2532	228	0.2005	4340	X-RAY DIFFRACTION	96.58
2.21-2.25	0.2276	228	0.1809	4336	X-RAY DIFFRACTION	96.17
2.25-2.29	0.2404	230	0.1807	4361	X-RAY DIFFRACTION	96.82
2.29-2.34	0.2298	225	0.1759	4283	X-RAY DIFFRACTION	96.12
2.34-2.39	0.2298	230	0.1738	4363	X-RAY DIFFRACTION	96.74
2.39-2.45	0.2318	228	0.1813	4337	X-RAY DIFFRACTION	96.8
2.45-2.51	0.2626	230	0.1845	4374	X-RAY DIFFRACTION	97.13
2.51-2.58	0.233	231	0.1807	4384	X-RAY DIFFRACTION	96.93
2.58-2.65	0.2434	227	0.1784	4305	X-RAY DIFFRACTION	97
2.65-2.74	0.2301	230	0.1705	4377	X-RAY DIFFRACTION	96.68
2.74-2.84	0.2114	231	0.1678	4388	X-RAY DIFFRACTION	96.71
2.84-2.95	0.1939	227	0.1688	4312	X-RAY DIFFRACTION	97.36
2.95-3.08	0.2065	231	0.1693	4379	X-RAY DIFFRACTION	97.4
3.08-3.25	0.1925	230	0.1702	4381	X-RAY DIFFRACTION	97.71
3.25-3.45	0.2112	233	0.1606	4417	X-RAY DIFFRACTION	97.71
3.45-3.71	0.1802	228	0.1569	4335	X-RAY DIFFRACTION	96.57
3.71-4.09	0.1827	227	0.1464	4322	X-RAY DIFFRACTION	94.99
4.09-4.68	0.1607	230	0.1389	4369	X-RAY DIFFRACTION	98.02
4.68-5.89	0.1964	228	0.1679	4337	X-RAY DIFFRACTION	96.96
5.89-33.26	0.1983	224	0.1925	4276	X-RAY DIFFRACTION	94.86

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.80243656717	-0.842967047	1.37378759504	5.28131351771	-2.44234486181	7.86647931659	0.00745383187627	-0.576984302534	0.1671798766	0.841802444918	0.117595786027	3.3345983272E-5	-0.382759776019	-0.227069489474	-0.100392688161	0.339754821821	-0.0209175256044	0.0620773032505	0.315827493594	-0.151291120269	0.31554362383	-3.5900585083	12.6422556356	34.3575682052
2	3.08252828118	-2.07414797706	1.26785273216	3.30024249722	-1.68945035357	1.1052150193	0.104075822241	0.141021894617	0.0453674338208	-0.195050925579	-0.0936022387417	-0.0825313306843	0.0157062883989	0.115844674411	-0.00762756922473	0.26612929733	0.0163189267001	0.0242288609426	0.351070374165	-0.0229004731936	0.23135072396	20.0638179343	-3.31256864018	20.6672493789
3	1.46332502603	0.211533039528	0.0960154509602	1.60450306749	-0.287134722403	1.46776032314	0.0211798345437	-0.0104458063898	0.0280102021475	0.00450062549297	-0.0223083192126	-0.0325959977169	0.0690896847419	-0.000637612036565	0.00260298758466	0.195050009772	0.0220464616911	0.00409709021087	0.235442164585	-0.00818883984769	0.221288294416	-2.09266221914	1.9591802805	21.0121807419
4	3.75439513761	-0.704577397474	-2.15396575413	3.9539757377	-0.249416413161	6.85523686376	0.195006242606	0.511983447865	0.371171417037	-0.697053613415	-0.0425472718262	0.185020296577	-0.74452592633	-0.334385625464	-0.126470785005	0.345999106916	0.0495400733676	-0.129832716665	0.32171815863	0.109521267002	0.363278782721	-5.62769366775	14.6487232638	-29.8371761784
5	1.97548521264	-1.21951574587	-1.10404976116	3.7077317447	1.37275253326	1.29669115927	-0.0713642249424	-0.119756437485	-0.0855439280754	0.101408856661	0.099083988807	0.0697389533136	0.116261820375	-0.0229693512533	-0.0370787901884	0.377105983613	-0.0216292423875	-0.0121218275847	0.252326695578	0.0281806021161	0.23739960886	4.06796840019	-15.3719143948	-17.2159409105
6	1.63663221948	-0.112347625275	-0.266323889517	1.07644751023	0.132920809795	1.22218626337	0.00124098284342	0.016893875685	-0.00529322806676	-0.0312572162061	0.0210886389008	0.054096184441	0.0108871739559	0.0244655098671	-0.0267089875524	0.22162570358	0.00503355979872	-0.0146147930712	0.136344742431	0.00544873347721	0.185343057179	4.58551483155	7.29158519857	-17.5991992879
7	3.19272922002	1.06358508242	-0.982688883404	3.63197444995	-0.644372414462	3.72306364557	0.279086710023	-0.606686433055	0.413168767963	0.135652749663	-0.342923925078	-0.248862191859	-0.762041949152	0.718433503248	0.0761316889742	0.493701020998	-0.194797262978	0.0368759629212	0.506387901218	-0.0531719669202	0.301036922408	22.7684779932	-3.93134574534	60.7222471177
8	4.51637366368	-0.159973423017	5.35393851755	2.87587892409	0.452523485738	6.43282132566	-0.327060459233	-1.32601293153	-0.320894734665	0.804388494081	-0.517767528747	-0.230910581965	-0.156448066758	0.343967281171	0.58175372273	0.600446188731	-0.158169062881	0.00374062299783	1.06857882103	0.247772741539	0.414498023531	10.4004338569	-21.517188588	73.8515063247
9	6.95320399138	0.883571121423	4.08247628078	3.40719875171	0.936083497219	2.45002422696	0.556586594074	-0.55142556174	-0.620590564808	0.783393269219	-0.353608378127	-0.429175849432	0.769166539128	-0.522936155143	-0.258018850694	0.362013685143	-0.115280183946	-0.0585944142182	0.265794220555	0.0191528989925	0.33164887952	-12.0313108415	-27.1656007293	50.280959637
10	6.37152731744	-0.193944267404	2.37776686878	2.03220917169	-1.63208078372	2.11505546051	-0.0125598113471	-0.067721501077	-0.277514092415	-0.022180831757	-0.000166114569334	-0.0633300657077	-0.0129349548327	-0.0281637210557	0.00413517336614	0.179751064386	0.00450577564397	0.0232872038831	0.187552001073	-0.0542514327791	0.227872585243	-6.45041835266	-23.7859059937	42.8890078976
11	5.77056132929	-0.698845589611	1.65990787769	3.19599902264	0.083501925474	4.24732753365	0.0241320632466	-0.0542542333954	-0.121804942154	0.143190275699	-0.105358260985	-0.00757228918867	-0.130844433149	-0.0792982598431	0.0328710546236	0.225397494376	-0.0100116711734	-0.0324966205917	0.189612895252	-0.01740990098	0.259736729264	-8.12482926302	-16.5294672767	45.4958097593
12	8.87160175422	0.176947558322	2.62307090031	2.75050626842	-0.168456829084	5.87635380524	-0.0273970575874	-0.0174407793514	-1.02556501304	-0.239528637301	-0.278400736477	-0.280533503587	0.458757690775	0.20293404843	0.271449232168	0.272734588936	-0.0187580733411	0.0294890056915	0.219441418936	0.0235073651369	0.519902316612	9.65366523277	-29.8053363955	49.9683274093
13	3.44236140906	0.442414849389	-0.551012495569	1.39629972715	0.424005524287	2.51151183451	0.0674849246976	-0.508072981048	-0.737867800854	0.258530259	-0.237226802012	-0.127042940073	0.195567070808	0.11971060267	0.129655221063	0.250951400969	-0.0181120815341	-0.0259028930616	0.346107199574	0.142955717424	0.336874078174	18.3401129655	-23.0766003444	56.5236630488
14	3.77300712308	0.367430047121	-2.57107082924	2.25267842334	-0.523347820035	3.48818240271	0.00954564735733	0.292113935865	-0.218101729022	-0.126619743322	-0.0980226396681	0.127684790645	0.00289241324388	-0.179011525137	0.085584647125	0.220165029728	0.0128654825407	-0.03093387222	0.312564534649	-0.00197745970539	0.220823147509	20.6593461477	-14.548322763	42.5025977843
15	2.8914287082	0.529921508077	-0.667095313244	2.61773413583	-0.235792002014	7.14072073278	0.390807944602	-0.965828612444	0.371600682287	0.581133937449	-0.327378490959	0.167863046	-0.849426570587	-0.00610146213099	-0.0759049111965	0.494800692611	-0.132636740654	0.0492125440463	0.531992781448	-0.108564888817	0.295119698097	15.8179661185	-5.78085820659	64.3228527993
16	2.55816826446	1.62459391781	-0.29493890137	1.5959117252	-0.456932195055	3.67048437884	-0.528666091779	0.777200076738	0.199794869164	-0.584558923684	0.375488756744	0.0133635198941	0.235224527899	-0.30072462793	0.14829428991	0.554587499809	-0.0799226576902	0.0167872429173	0.545833975349	0.0867273470489	0.331657427821	13.8027304032	-14.8842064009	-63.783787995
17	4.20603148131	0.467270577483	0.760685632025	4.11557020703	1.56073995317	4.0060799282	-0.0445750189531	0.25245129202	-0.191601624632	-0.0203451290457	0.0244740633903	-0.0324912694545	0.0332551330541	0.0638021863074	0.0115556559341	0.219332921184	-0.0245933792997	-0.0234507143046	0.177199449007	0.0290777027453	0.245955234524	29.3593196549	7.2109499139	-41.9665195972
18	2.73054121029	0.574195080652	-0.30326656806	3.11088890697	-0.56436464517	2.50448584179	-0.169836613849	0.310134406	0.0919887306956	-0.211404843157	0.0422526713119	-0.355984517211	-0.0193526817753	0.0114576074145	0.118567888973	0.285101551702	-0.0309171041912	0.0311492937234	0.196848527725	-0.00818507332824	0.231231919374	20.8414863924	-16.9115890186	-51.7164927997
19	3.37184236269	-1.08433486185	-2.27776796844	1.19328413065	2.31920309997	4.63145108732	0.922059039308	0.544638590434	-0.320049828321	-1.29078753797	0.00204717951259	0.303114674261	0.106747121183	0.356586180268	-0.896938608993	1.84834678008	0.512222199166	-0.0320479135467	1.30705830852	0.11169098473	0.696311318612	9.09743948414	-7.05880909616	-25.6715053802
20	9.30709547008	-5.27988923227	-3.6615680231	3.63413954416	0.216862182758	6.82511264872	-0.466242382232	0.395231231568	0.567793830361	-1.37529313591	-0.251092171729	2.5167759382	-1.44757089027	-0.900124936709	0.6027198206	1.16779526292	-0.108009359537	-0.611877676333	0.866806810531	0.229697319914	1.79745119195	4.44814137333	-15.5434576891	44.6513682549
21	7.34270465225	1.6781573628	-1.95073947867	2.27393885499	-0.214678777488	0.547161961017	0.168608821538	-1.58782813847	-0.0765473565543	1.23175119808	1.02929360944	-0.246085056086	1.32550090431	-1.25538587167	-1.13162476998	1.0023112343	0.230933620972	-0.0127278451259	1.25418206853	0.123892301873	0.581980119758	10.6669347337	-5.91376940387	28.9403843649
22	6.23540635112	0.468735299942	0.161456337839	1.35838435764	-1.14699244844	1.46275338742	0.0281034630043	0.0262518565697	0.382234002232	-0.0556256421163	-0.397318116378	0.826821582743	0.291409164916	-0.497756328169	0.341843906236	0.695144980771	-0.145682965656	0.0195928972362	1.1606789417	-0.219950472545	0.955718939655	19.9365532282	-3.33783086379	-41.2472164315

Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

ID	Refine TLS-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	chain 'A' and (resid 245 through 273 )	A	A	245 - 273	1 - 31
2	2	chain 'A' and (resid 274 through 397 )	A	A	274 - 397	32 - 163
3	3	chain 'A' and (resid 398 through 598 )	A	A	398 - 598	164 - 374
4	4	chain 'B' and (resid 246 through 273 )	B	B	246 - 273	1 - 30
5	5	chain 'B' and (resid 274 through 397 )	B	B	274 - 397	31 - 156
6	6	chain 'B' and (resid 398 through 598 )	B	B	398 - 598	157 - 361
7	7	chain 'C' and (resid 247 through 273 )	C	C	247 - 273	1 - 27
8	8	chain 'C' and (resid 274 through 294 )	C	C	274 - 294	28 - 50
9	9	chain 'C' and (resid 295 through 315 )	C	C	295 - 315	51 - 71
10	10	chain 'C' and (resid 316 through 363 )	C	C	316 - 363	72 - 119
11	11	chain 'C' and (resid 364 through 397 )	C	C	364 - 397	120 - 153
12	12	chain 'C' and (resid 398 through 413 )	C	C	398 - 413	154 - 169
13	13	chain 'C' and (resid 414 through 534 )	C	C	414 - 534	170 - 288
14	14	chain 'C' and (resid 535 through 564 )	C	C	535 - 564	289 - 322
15	15	chain 'C' and (resid 565 through 597 )	C	C	565 - 597	323 - 355
16	16	chain 'D' and (resid 248 through 294 )	D	D	248 - 294	1 - 49
17	17	chain 'D' and (resid 295 through 397 )	D	D	295 - 397	50 - 154
18	18	chain 'D' and (resid 398 through 597 )	D	D	398 - 597	155 - 362
19	19	chain 'E' and (resid -2 through 0 )	E	E	-2 - 0
20	20	chain 'I' and (resid -2 through 0 )	I	F	-2 - 0
21	21	chain 'F' and (resid -2 through 0 )	F	G	-2 - 0
22	22	chain 'K' and (resid -2 through 0 )	K	H	-2 - 0

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