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- PDB-5w0m: Structure of human TUT7 catalytic module (CM) in complex with U5 RNA -

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Basic information

Entry
Database: PDB / ID: 5w0m
TitleStructure of human TUT7 catalytic module (CM) in complex with U5 RNA
Components
  • Terminal uridylyltransferase 7
  • U5 single-stranded RNA
KeywordsTRANSFERASE/RNA / terminal uridyltransferase / TUTase / TRANSFERASE-RNA complex
Function / homology
Function and homology information


polyuridylation-dependent mRNA catabolic process / uridylyltransferase activity / transposable element silencing by mRNA destabilization / RNA uridylyltransferase / RNA 3'-end processing / RNA uridylyltransferase activity / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / miRNA metabolic process / Deadenylation of mRNA / pre-miRNA processing ...polyuridylation-dependent mRNA catabolic process / uridylyltransferase activity / transposable element silencing by mRNA destabilization / RNA uridylyltransferase / RNA 3'-end processing / RNA uridylyltransferase activity / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / miRNA metabolic process / Deadenylation of mRNA / pre-miRNA processing / oocyte maturation / miRNA binding / Zygotic genome activation (ZGA) / RNA binding / zinc ion binding / nucleoplasm / cytosol / cytoplasm
Similarity search - Function
Unstructured region 4 on terminal uridylyltransferase 7 / TUTase nucleotidyltransferase domain / TUTase nucleotidyltransferase domain / PAP/25A-associated / Cid1 family poly A polymerase / : / Poly(A) RNA polymerase, mitochondrial-like, central palm domain / Matrin/U1-C-like, C2H2-type zinc finger / U1-like zinc finger / Nucleotidyltransferase superfamily ...Unstructured region 4 on terminal uridylyltransferase 7 / TUTase nucleotidyltransferase domain / TUTase nucleotidyltransferase domain / PAP/25A-associated / Cid1 family poly A polymerase / : / Poly(A) RNA polymerase, mitochondrial-like, central palm domain / Matrin/U1-C-like, C2H2-type zinc finger / U1-like zinc finger / Nucleotidyltransferase superfamily / Zinc finger C2H2 type domain signature. / zinc finger / Zinc knuckle / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile.
Similarity search - Domain/homology
IODIDE ION / RNA / Terminal uridylyltransferase 7
Similarity search - Component
Biological speciesHomo sapiens (human)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.298 Å
AuthorsFaehnle, C.R. / Walleshauser, J. / Joshua-Tor, L.
CitationJournal: Nat. Struct. Mol. Biol. / Year: 2017
Title: Multi-domain utilization by TUT4 and TUT7 in control of let-7 biogenesis.
Authors: Faehnle, C.R. / Walleshauser, J. / Joshua-Tor, L.
History
DepositionMay 31, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 28, 2017Provider: repository / Type: Initial release
Revision 1.1Jul 19, 2017Group: Database references / Category: citation
Item: _citation.journal_abbrev / _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.2Aug 16, 2017Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Oct 4, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Terminal uridylyltransferase 7
B: Terminal uridylyltransferase 7
C: Terminal uridylyltransferase 7
H: U5 single-stranded RNA
I: U5 single-stranded RNA
J: U5 single-stranded RNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)139,64616
Polymers138,6236
Non-polymers1,02310
Water3,693205
1
A: Terminal uridylyltransferase 7
H: U5 single-stranded RNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)46,5926
Polymers46,2082
Non-polymers3844
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2180 Å2
ΔGint-46 kcal/mol
Surface area18400 Å2
MethodPISA
2
B: Terminal uridylyltransferase 7
I: U5 single-stranded RNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)46,4314
Polymers46,2082
Non-polymers2232
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1930 Å2
ΔGint-40 kcal/mol
Surface area16890 Å2
MethodPISA
3
C: Terminal uridylyltransferase 7
J: U5 single-stranded RNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)46,6236
Polymers46,2082
Non-polymers4154
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2210 Å2
ΔGint-42 kcal/mol
Surface area18080 Å2
MethodPISA
Unit cell
Length a, b, c (Å)135.472, 135.472, 179.268
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number169
Space group name H-MP61
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and ((resid 988 and (name N or name...
21(chain C and (resseq 988:992 or resseq 994:997 or resseq...
12chain H
22chain I
32chain J

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111GLUGLUGLUGLU(chain A and ((resid 988 and (name N or name...AA98812
121LEULEUARGARG(chain A and ((resid 988 and (name N or name...AA987 - 136311 - 387
131LEULEUARGARG(chain A and ((resid 988 and (name N or name...AA987 - 136311 - 387
141LEULEUARGARG(chain A and ((resid 988 and (name N or name...AA987 - 136311 - 387
151LEULEUARGARG(chain A and ((resid 988 and (name N or name...AA987 - 136311 - 387
161LEULEUARGARG(chain A and ((resid 988 and (name N or name...AA987 - 136311 - 387
171LEULEUARGARG(chain A and ((resid 988 and (name N or name...AA987 - 136311 - 387
181LEULEUARGARG(chain A and ((resid 988 and (name N or name...AA987 - 136311 - 387
191LEULEUARGARG(chain A and ((resid 988 and (name N or name...AA987 - 136311 - 387
1101LEULEUARGARG(chain A and ((resid 988 and (name N or name...AA987 - 136311 - 387
1111LEULEUARGARG(chain A and ((resid 988 and (name N or name...AA987 - 136311 - 387
1121LEULEUARGARG(chain A and ((resid 988 and (name N or name...AA987 - 136311 - 387
1131LEULEUARGARG(chain A and ((resid 988 and (name N or name...AA987 - 136311 - 387
1141LEULEUARGARG(chain A and ((resid 988 and (name N or name...AA987 - 136311 - 387
211GLUGLUPROPRO(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC988 - 99212 - 16
221THRTHRPHEPHE(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC994 - 99718 - 21
231ASNASNGLUGLU(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC999 - 102323 - 47
241ILEILEGLNGLN(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC1025 - 103549 - 59
251ASPASPASPASP(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC103660
261GLUGLUMETMET(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC988 - 136212 - 386
271GLUGLUMETMET(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC988 - 136212 - 386
281GLUGLUMETMET(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC988 - 136212 - 386
291GLUGLUMETMET(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC988 - 136212 - 386
2101GLUGLUMETMET(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC988 - 136212 - 386
2111GLUGLUMETMET(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC988 - 136212 - 386
2121GLUGLUMETMET(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC988 - 136212 - 386
2131GLUGLUMETMET(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC988 - 136212 - 386
2141GLUGLUMETMET(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC988 - 136212 - 386
2151GLUGLUMETMET(chain C and (resseq 988:992 or resseq 994:997 or resseq...CC988 - 136212 - 386
112UUUUchain HHD1 - 41 - 4
212UUUUchain IIE1 - 41 - 4
312UUUUchain JJF1 - 41 - 4

NCS ensembles :
ID
1
2

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Components

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Protein / RNA chain , 2 types, 6 molecules ABCHIJ

#1: Protein Terminal uridylyltransferase 7 / TUT7 / TUTase 7 / Zinc finger CCHC domain-containing protein 6


Mass: 44721.812 Da / Num. of mol.: 3
Fragment: nucleotidyltransferase domain (UNP residues 983-1365)
Mutation: D1160A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ZCCHC6, HS2, KIAA1711, TUT7 / Plasmid: Multi-Bac, pFL / Production host: Spodoptera frugiperda (fall armyworm) / Strain (production host): Sf9 / References: UniProt: Q5VYS8, RNA uridylyltransferase
#2: RNA chain U5 single-stranded RNA


Mass: 1485.872 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Non-polymers , 4 types, 215 molecules

#3: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: SO4
#4: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#5: Chemical
ChemComp-IOD / IODIDE ION


Mass: 126.904 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: I
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 205 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.42 Å3/Da / Density % sol: 64.06 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop
Details: 0.8-1.0 M lithium sulfate, 0.075-0.2 M potassium iodide, 0.05-0.2 M trisodium citrate, pH 5-6
PH range: 5.0-6.0

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 1 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Jan 16, 2015
RadiationMonochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.298→179 Å / Num. obs: 82731 / % possible obs: 99.9 % / Redundancy: 5.6 % / Biso Wilson estimate: 43.65 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.049 / Net I/σ(I): 22.7
Reflection shellResolution: 2.298→2.31 Å / Rmerge(I) obs: 0.556 / CC1/2: 0.784

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Processing

Software
NameVersionClassification
Aimlessdata scaling
PHENIX1.10_2155refinement
PDB_EXTRACT3.22data extraction
XDSdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 5W0B

5w0b


Resolution: 2.298→98.168 Å / SU ML: 0.3 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 25.33
RfactorNum. reflection% reflection
Rfree0.2287 4139 5.01 %
Rwork0.1883 --
obs0.1904 82655 99.84 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 204.5 Å2 / Biso mean: 65.9348 Å2 / Biso min: 20.33 Å2
Refinement stepCycle: final / Resolution: 2.298→98.168 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8863 231 26 205 9325
Biso mean--61.54 52.3 -
Num. residues----1105
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0059355
X-RAY DIFFRACTIONf_angle_d0.68312680
X-RAY DIFFRACTIONf_chiral_restr0.0441405
X-RAY DIFFRACTIONf_plane_restr0.0041573
X-RAY DIFFRACTIONf_dihedral_angle_d11.3025593
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A4430X-RAY DIFFRACTION7.128TORSIONAL
12C4430X-RAY DIFFRACTION7.128TORSIONAL
21H147X-RAY DIFFRACTION7.128TORSIONAL
22I147X-RAY DIFFRACTION7.128TORSIONAL
23J147X-RAY DIFFRACTION7.128TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.2976-2.32370.30961380.259625892727100
2.3237-2.35110.29471320.254126232755100
2.3511-2.37970.29621310.234526362767100
2.3797-2.40990.2831230.232626162739100
2.4099-2.44160.27951270.230726372764100
2.4416-2.4750.27641380.227325952733100
2.475-2.51040.2721280.236626272755100
2.5104-2.54790.3161410.230325962737100
2.5479-2.58770.28631490.230126212770100
2.5877-2.63010.31451530.230325982751100
2.6301-2.67540.30871440.223626152759100
2.6754-2.72410.27671500.21825932743100
2.7241-2.77650.24781410.2226192760100
2.7765-2.83320.29891520.20725862738100
2.8332-2.89480.26641310.210626512782100
2.8948-2.96210.28761130.216826182731100
2.9621-3.03620.27811450.224326462791100
3.0362-3.11830.2751280.231526012729100
3.1183-3.21010.29381440.224226352779100
3.2101-3.31370.28841410.213126212762100
3.3137-3.43210.23631360.20225612697100
3.4321-3.56960.25441420.191626692811100
3.5696-3.7320.22481450.186126012746100
3.732-3.92880.19781490.165626092758100
3.9288-4.17490.18591410.15222604274599
4.1749-4.49730.15911230.142326242747100
4.4973-4.94980.15341320.140926162748100
4.9498-5.6660.191390.16092623276299
5.666-7.13830.20171310.186726512782100
7.1383-98.25760.17921520.16612635278799
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.7295-0.75466.63581.61840.25487.8342-0.00820.6078-0.11810.2141-0.1075-0.48250.01271.42080.14340.2685-0.0193-0.0680.44430.03120.39635.5344-53.215516.526
22.2464-5.41289.31753.658-2.32747.6182-0.08190.54350.0926-0.12940.20150.123-0.64250.06010.02870.3908-0.032-0.01330.48230.16350.4385-16.3144-43.4283-10.0278
39.0017-0.89874.76993.5645-1.93954.961-0.28540.27790.4436-0.22210.12070.4806-0.4668-0.22860.15080.3370.0571-0.05510.41630.08870.4169-22.4415-40.7737-0.8562
43.3408-1.8318-1.99935.79944.035.8553-0.02180.2960.1117-0.2868-0.09860.4879-0.0917-0.74810.08190.312-0.0092-0.05770.45390.1720.4237-27.5438-50.3638-3.8334
54.0139-0.06220.4194.22020.41812.9882-0.1118-0.37350.5980.3797-0.01310.2868-0.599-0.27410.12450.37180.031-0.00370.3532-0.03380.3198-11.7606-40.948419.6372
63.7577-1.07030.97183.29130.83697.8306-0.1119-0.702-0.59510.41070.1632-0.22620.84520.55170.06020.45340.0001-0.07250.30470.07250.3247-2.0666-61.756122.3437
72.66333.30933.87792.8455-0.40865.3040.3818-0.5809-0.46060.07030.08530.29030.3156-0.7464-0.43970.44820.07570.0290.42260.04130.3739-18.323-64.56412.5442
82.2996-0.4512-1.88034.22820.54982.68550.19730.59310.2975-0.2751-0.3499-0.87-0.07990.82910.10510.31420.04730.04180.72960.09370.456218.8157-66.9682-3.6409
92.15716.52572.62745.92921.28171.30650.5188-0.62070.33570.5696-0.40150.21240.1002-0.19020.00120.30850.02740.01590.34870.01750.3409-15.7706-68.7285.3469
105.63851.7209-0.79971.8457-0.12344.21660.01620.0495-0.3028-0.1198-0.04180.0080.4947-0.1364-0.00140.32090.034-0.0280.2290.01090.2807-13.8081-79.58694.0313
114.9609-3.09411.056410.0105-3.52715.96650.16380.5959-0.8298-0.75080.27210.68181.1514-0.5633-0.42060.4685-0.0989-0.08480.4702-0.06270.3561-21.1232-80.0254-6.9855
123.45430.1466-0.24531.1019-1.18932.3584-0.16010.0721-0.1507-0.09480.0127-0.11220.31710.23420.16560.34330.06380.04040.3059-0.02940.2605-1.5921-75.96850.581
132.08210.7341-0.55512.1396-1.1262.5717-0.0028-0.174-0.33320.0223-0.3151-0.41610.57360.84390.30380.43470.24650.07180.54130.07090.448810.8536-83.78145.7469
144.66-0.5557-2.23861.63521.84086.72830.33770.2656-0.828-0.6302-0.1929-0.69951.64980.933-0.10250.92190.4630.19730.68010.04820.756113.3856-95.2636-1.4959
155.22290.10571.26.517-2.95861.6793-0.25110.9349-1.0528-0.95720.0954-0.62031.63210.18730.29510.76150.1290.17870.4949-0.11050.56454.1677-93.6189-5.3285
166.69966.63254.6858.24222.41615.9315-0.24630.7627-0.5214-1.0043-0.0034-0.7810.47980.58950.27690.67430.26870.23160.8978-0.010.498614.8246-80.8103-15.8902
176.52174.32390.85445.4812-0.00353.975-0.19180.5934-0.1944-0.8369-0.1265-0.51020.32940.57870.32720.51490.23040.13390.75050.13850.487311.0665-72.5959-17.5735
184.92785.93112.56749.92714.51954.19930.4387-0.62090.0130.8395-0.3099-0.5452-0.1180.4657-0.14250.3381-0.1157-0.07560.6242-0.00530.387712.1027-40.6094-6.0675
198.17563.93528.35915.68465.58638.90060.3426-0.1366-0.42220.4121-0.13580.01370.9187-0.0408-0.25050.38090.01750.10780.28180.04940.3431-3.2385-68.3107-21.9902
204.74261.97895.02023.68684.81928.32810.07590.5147-0.32840.04270.1004-0.26590.36150.2531-0.14480.27990.01150.07770.3461-0.01370.3016-2.1075-61.2352-30.6631
215.19330.6296-1.46954.7993-0.86535.4949-0.09210.4362-0.1318-0.0280.12540.49260.0168-0.6338-0.02230.24610.03330.01380.3874-0.0410.3236-12.9581-59.7163-28.5351
221.4950.0578-1.20742.89941.43786.7187-0.0535-0.0208-0.1862-0.18520.0841-0.45150.02070.9562-0.03770.2178-0.0125-0.00170.39390.03510.331512.3309-47.6245-27.2126
235.05660.03410.21382.79372.05396.60930.01490.13830.5954-0.5084-0.0553-0.5357-1.06421.06220.03560.4958-0.21950.06640.51340.04950.452315.2086-35.3676-25.8196
248.1571.1613-4.7212.58912.37886.86570.39270.80430.6461-1.6642-0.25220.6124-1.6343-0.3437-0.18160.62990.0207-0.09390.43160.07350.35622.4523-37.7167-35.0366
252.31891.79038.72124.54751.38412.2826-0.2091-0.24480.6992-0.096-0.10430.1206-1.2461-0.27780.50570.5058-0.0182-0.00370.4905-0.0230.45531.0473-29.5764-16.9673
268.75310.3928-1.16196.2835-4.41536.31610.116-1.5467-0.01811.3276-0.1547-0.1744-1.30330.11630.0660.5686-0.13370.01190.6397-0.0970.37833.5477-35.8065-1.383
276.03525.86416.05119.49246.05212.5036-0.1664-0.57410.6203-0.3983-0.38781.3332-0.4246-1.03270.51440.55520.1175-0.06110.630.03460.5229-16.5489-39.0471-25.0875
286.42741.96335.26554.5934.86858.8333-0.4912-0.54610.30240.93310.29860.05260.0754-0.60740.25860.38560.1131-0.00250.49740.11530.5839-24.5533-50.002410.7494
293.1304-3.91674.84794.8788-6.03147.4542-0.2271.561-1.2436-1.26110.2947-0.49080.42061.406-0.07170.5141-0.08860.11460.7255-0.13890.4923-5.7538-85.3499-7.2865
307.21713.1216-3.41837.25174.6237.9544-0.3587-0.16861.3055-0.46450.31370.663-1.1108-0.13780.17370.43030.0534-0.10.3802-0.07290.5025-5.4817-46.9094-30.3304
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 987 through 1015 )A987 - 1015
2X-RAY DIFFRACTION2chain 'A' and (resid 1016 through 1035 )A0
3X-RAY DIFFRACTION3chain 'A' and (resid 1036 through 1075 )A0
4X-RAY DIFFRACTION4chain 'A' and (resid 1076 through 1124 )A0
5X-RAY DIFFRACTION5chain 'A' and (resid 1125 through 1293 )A0
6X-RAY DIFFRACTION6chain 'A' and (resid 1294 through 1324 )A0
7X-RAY DIFFRACTION7chain 'A' and (resid 1325 through 1363 )A0
8X-RAY DIFFRACTION8chain 'B' and (resid 988 through 1015 )B988 - 1015
9X-RAY DIFFRACTION9chain 'B' and (resid 1016 through 1035 )B0
10X-RAY DIFFRACTION10chain 'B' and (resid 1036 through 1075 )B0
11X-RAY DIFFRACTION11chain 'B' and (resid 1076 through 1105 )B0
12X-RAY DIFFRACTION12chain 'B' and (resid 1106 through 1157 )B0
13X-RAY DIFFRACTION13chain 'B' and (resid 1158 through 1247 )B0
14X-RAY DIFFRACTION14chain 'B' and (resid 1248 through 1269 )B0
15X-RAY DIFFRACTION15chain 'B' and (resid 1270 through 1293 )B0
16X-RAY DIFFRACTION16chain 'B' and (resid 1294 through 1312 )B0
17X-RAY DIFFRACTION17chain 'B' and (resid 1313 through 1347 )B0
18X-RAY DIFFRACTION18chain 'C' and (resid 988 through 1015 )C988 - 1015
19X-RAY DIFFRACTION19chain 'C' and (resid 1016 through 1035 )C0
20X-RAY DIFFRACTION20chain 'C' and (resid 1036 through 1075 )C0
21X-RAY DIFFRACTION21chain 'C' and (resid 1076 through 1124 )C0
22X-RAY DIFFRACTION22chain 'C' and (resid 1125 through 1223 )C0
23X-RAY DIFFRACTION23chain 'C' and (resid 1224 through 1269 )C0
24X-RAY DIFFRACTION24chain 'C' and (resid 1270 through 1293 )C0
25X-RAY DIFFRACTION25chain 'C' and (resid 1294 through 1312 )C0
26X-RAY DIFFRACTION26chain 'C' and (resid 1313 through 1335 )C0
27X-RAY DIFFRACTION27chain 'C' and (resid 1336 through 1362 )C0
28X-RAY DIFFRACTION28chain 'H' and (resid 1 through 4 )H1 - 4
29X-RAY DIFFRACTION29chain 'I' and (resid 1 through 4 )I1 - 4
30X-RAY DIFFRACTION30chain 'J' and (resid 1 through 4 )J1 - 4

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