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- PDB-6ylv: Translation initiation factor 4E in complex with 4-Cl-Bn7GpppG mR... -

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Basic information

Entry
Database: PDB / ID: 6ylv
TitleTranslation initiation factor 4E in complex with 4-Cl-Bn7GpppG mRNA 5' cap analog
ComponentsEukaryotic translation initiation factor 4E
KeywordsTRANSLATION / cap analog / translation initiation factor / 4-Cl-Bn7GpppG / protein-ligand complex / eIF4E / RNA binding protein
Function / homology
Function and homology information


Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / Transport of Mature mRNA Derived from an Intronless Transcript / Deadenylation of mRNA / Translation initiation complex formation / Ribosomal scanning and start codon recognition / ISG15 antiviral mechanism / mTORC1-mediated signalling / L13a-mediated translational silencing of Ceruloplasmin expression ...Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / Transport of Mature mRNA Derived from an Intronless Transcript / Deadenylation of mRNA / Translation initiation complex formation / Ribosomal scanning and start codon recognition / ISG15 antiviral mechanism / mTORC1-mediated signalling / L13a-mediated translational silencing of Ceruloplasmin expression / GTP hydrolysis and joining of the 60S ribosomal subunit / eukaryotic initiation factor 4G binding / regulation of translation at postsynapse, modulating synaptic transmission / chromatoid body / eukaryotic translation initiation factor 4F complex / mRNA cap binding / RNA 7-methylguanosine cap binding / nuclear export / RISC complex / postsynaptic cytosol / stem cell population maintenance / negative regulation of neuron differentiation / behavioral fear response / mRNA export from nucleus / translational initiation / translation initiation factor activity / positive regulation of mitotic cell cycle / cellular response to dexamethasone stimulus / P-body / neuron differentiation / cytoplasmic stress granule / G1/S transition of mitotic cell cycle / regulation of translation / postsynapse / DNA-binding transcription factor binding / nuclear body / negative regulation of translation / nuclear speck / translation / glutamatergic synapse / perinuclear region of cytoplasm / enzyme binding / protein-containing complex / nucleus / cytoplasm / cytosol
Similarity search - Function
RNA Cap, Translation Initiation Factor Eif4e / RNA Cap, Translation Initiation Factor Eif4e / Eukaryotic translation initiation factor 4E (eIF-4E), conserved site / Eukaryotic initiation factor 4E signature. / Translation Initiation factor eIF- 4e / Eukaryotic initiation factor 4E / Translation Initiation factor eIF- 4e-like / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
4-Cl-Bn7GpppG mRNA 5' cap analog / Eukaryotic translation initiation factor 4E
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.66005667871 Å
AuthorsKubacka, D. / Wojcik, R. / Baranowski, M.R. / Kowalska, J. / Jemielity, J.
Funding support Poland, 2items
OrganizationGrant numberCountry
Foundation for Polish ScienceTEAM/2016_2/13 Poland
Polish National Science CentreUMO- 2017/24/C/NZ1/00169 Poland
Citation
Journal: Pharmaceutics / Year: 2021
Title: Novel N7-Arylmethyl Substituted Dinucleotide mRNA 5' cap Analogs: Synthesis and Evaluation as Modulators of Translation.
Authors: Wojcik, R. / Baranowski, M.R. / Markiewicz, L. / Kubacka, D. / Bednarczyk, M. / Baran, N. / Wojtczak, A. / Sikorski, P.J. / Zuberek, J. / Kowalska, J. / Jemielity, J.
History
DepositionApr 7, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jun 10, 2020Provider: repository / Type: Initial release
Revision 1.1Dec 29, 2021Group: Database references / Category: citation / citation_author / database_2
Item: _citation.journal_abbrev / _citation.journal_id_CSD ..._citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.2Jan 24, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / citation / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _citation.country / _struct_ncs_dom_lim.beg_auth_comp_id ..._citation.country / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Eukaryotic translation initiation factor 4E
B: Eukaryotic translation initiation factor 4E
C: Eukaryotic translation initiation factor 4E
D: Eukaryotic translation initiation factor 4E
hetero molecules


Theoretical massNumber of molelcules
Total (without water)92,94510
Polymers89,1054
Non-polymers3,8406
Water4,288238
1
A: Eukaryotic translation initiation factor 4E
hetero molecules


Theoretical massNumber of molelcules
Total (without water)23,2823
Polymers22,2761
Non-polymers1,0062
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Eukaryotic translation initiation factor 4E
hetero molecules


Theoretical massNumber of molelcules
Total (without water)23,2823
Polymers22,2761
Non-polymers1,0062
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Eukaryotic translation initiation factor 4E
hetero molecules


Theoretical massNumber of molelcules
Total (without water)23,1902
Polymers22,2761
Non-polymers9141
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Eukaryotic translation initiation factor 4E
hetero molecules


Theoretical massNumber of molelcules
Total (without water)23,1902
Polymers22,2761
Non-polymers9141
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)38.350, 38.390, 148.080
Angle α, β, γ (deg.)85.276, 88.416, 77.234
Int Tables number1
Space group name H-MP1
Space group name HallP1
Symmetry operation#1: x,y,z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
31
41

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11GLUGLULYSLYS(chain 'A' and (resid 32 through 36 or resid 38...AA32 - 366 - 10
12PROPROMETMET(chain 'A' and (resid 32 through 36 or resid 38...AA38 - 10112 - 75
13GLUGLULYSLYS(chain 'A' and (resid 32 through 36 or resid 38...AA103 - 11977 - 93
14GLNGLNALAALA(chain 'A' and (resid 32 through 36 or resid 38...AA121 - 20195 - 175
15LYSLYSVALVAL(chain 'A' and (resid 32 through 36 or resid 38...AA212 - 217186 - 191
26GLUGLULYSLYS(chain 'B' and (resid 32 through 36 or resid 38...BB32 - 366 - 10
27PROPROMETMET(chain 'B' and (resid 32 through 36 or resid 38...BB38 - 10112 - 75
28GLUGLULYSLYS(chain 'B' and (resid 32 through 36 or resid 38...BB103 - 11977 - 93
29GLNGLNALAALA(chain 'B' and (resid 32 through 36 or resid 38...BB121 - 20195 - 175
210LYSLYSVALVAL(chain 'B' and (resid 32 through 36 or resid 38...BB212 - 217186 - 191
311GLUGLULYSLYS(chain 'C' and (resid 32 through 36 or resid 38...CC32 - 366 - 10
312PROPROMETMET(chain 'C' and (resid 32 through 36 or resid 38...CC38 - 10112 - 75
313GLUGLULYSLYS(chain 'C' and (resid 32 through 36 or resid 38...CC103 - 11977 - 93
314GLNGLNALAALA(chain 'C' and (resid 32 through 36 or resid 38...CC121 - 20195 - 175
315LYSLYSVALVAL(chain 'C' and (resid 32 through 36 or resid 38...CC212 - 217186 - 191
416GLUGLULYSLYS(chain 'D' and ((resid 32 and (name N or name...DD32 - 366 - 10
417PROPROMETMET(chain 'D' and ((resid 32 and (name N or name...DD38 - 10112 - 75
418GLUGLULYSLYS(chain 'D' and ((resid 32 and (name N or name...DD103 - 11977 - 93
419GLNGLNALAALA(chain 'D' and ((resid 32 and (name N or name...DD121 - 20195 - 175
420LYSLYSVALVAL(chain 'D' and ((resid 32 and (name N or name...DD212 - 217186 - 191

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Components

#1: Protein
Eukaryotic translation initiation factor 4E / mRNA cap-binding protein / eIF-4F 25 kDa subunit


Mass: 22276.309 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Eif4e / Production host: Escherichia coli (E. coli) / References: UniProt: P63073
#2: Chemical
ChemComp-OYW / 4-Cl-Bn7GpppG mRNA 5' cap analog / [[(2~{R},3~{S},4~{R},5~{R})-5-[2-azanyl-7-[(4-chlorophenyl)methyl]-6-oxidanylidene-1~{H}-purin-9-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate


Type: RNA linking / Mass: 913.981 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C27H33ClN10O18P3 / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C3H8O3
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 238 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.38 Å3/Da / Density % sol: 48.28 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8.5
Details: 0.1 M Bicine pH 8.5, 0.2 M sodium formate, 19 % w/v PEGMME 5000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: BESSY / Beamline: 14.1 / Wavelength: 0.9184 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jan 19, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9184 Å / Relative weight: 1
ReflectionResolution: 2.66→50 Å / Num. obs: 22090 / % possible obs: 93.59 % / Redundancy: 4.7 % / Biso Wilson estimate: 37.7561760788 Å2 / CC1/2: 0.991 / CC star: 0.998 / Rmerge(I) obs: 0.1122 / Rpim(I) all: 0.0555 / Rrim(I) all: 0.1258 / Net I/σ(I): 10.32
Reflection shellResolution: 2.66→2.755 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.571 / Mean I/σ(I) obs: 2.04 / Num. unique obs: 1453 / CC1/2: 0.764 / CC star: 0.931 / Rpim(I) all: 0.3611 / Rrim(I) all: 0.6803 / % possible all: 60.42

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Processing

Software
NameVersionClassification
PHENIX1.11.1_2575refinement
XDSdata reduction
XDSdata scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1L8B

1l8b


Resolution: 2.66005667871→49.1900006675 Å / SU ML: 0.343454744046 / Cross valid method: NONE / σ(F): 1.9795919456 / Phase error: 27.0057895497
RfactorNum. reflection% reflection
Rfree0.255269123128 814 3.68642724514 %
Rwork0.201483739807 --
obs0.203474516796 22081 93.6032217041 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 44.7187982624 Å2
Refinement stepCycle: LAST / Resolution: 2.66005667871→49.1900006675 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5750 0 172 242 6164
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.006181322382736233
X-RAY DIFFRACTIONf_angle_d0.7466520855938545
X-RAY DIFFRACTIONf_chiral_restr0.360645201754907
X-RAY DIFFRACTIONf_plane_restr0.003371226675911056
X-RAY DIFFRACTIONf_dihedral_angle_d10.86294564574494
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.6601-2.82670.3098650882541050.2377499792542738X-RAY DIFFRACTION72.4515800204
2.8267-3.04490.3289007435041400.2434324634493647X-RAY DIFFRACTION97.0030737705
3.0449-3.35130.2823116601961410.2201676735093683X-RAY DIFFRACTION96.9574036511
3.3513-3.83610.2709521567041430.1963627450223739X-RAY DIFFRACTION97.2932330827
3.8361-4.83240.2233082317781440.1708552012583759X-RAY DIFFRACTION99.617151608
4.8324-49.190.2197753979341410.2016123138093701X-RAY DIFFRACTION98.2860066513
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.25536361395-0.6070713515910.1424051777911.01282487348-1.091586931432.234574680050.192050071310.343317009755-0.546715410996-0.2944202066790.1336190266270.3647928655390.393689530832-0.247093776645-0.2163989044770.163194261158-0.208722209139-0.04954865717180.3574711462450.1396058318230.483151232893-69.463377241659.81653667861.16480038177
21.09224766831-0.448374063636-0.2013359858970.189340332870.04386391984530.3856143691680.1636972960190.1369417545030.470618927666-0.07052951274990.102298986141-0.0902130616907-0.1769930586270.1507706159390.623667667078-0.009231118931970.0630830296518-0.144195985060.1683407466140.1733229867290.519444124651-56.25505140584.42389074617.57486945293
32.47769568370.145020257862-0.5541888407761.367447029030.2195745683423.085076476350.02340717852380.2626293734290.202798208806-0.2948546012280.0282627328697-0.15066471195-0.04661738237550.249564204224-0.2903728013530.125641753261-0.00406249125402-0.08899793013140.213362466670.03688125812070.44430872214-53.762138786972.95903041643.90768863141
44.693205660131.765145195783.459018402916.373896184252.745258452193.70549752560.000125602677245-0.483949583320.206323474859-0.2423767023010.0290214728712-0.283564523753-0.484771413841-0.164890365028-0.07656979462860.237990324726-0.0419387586362-0.05103097914280.281840414209-0.05534799648240.403233579585-63.125022553481.624203120819.9213490179
50.7880381344540.482257487532-0.0642649838080.31360347463-0.1253756960750.38058701693-0.0336091662186-0.194711384043-0.0962552854487-0.00444123724548-0.169938117622-0.07613062357770.2407750819450.0638261307878-0.3057818475850.134680506957-0.100585564098-0.01895084521430.2265227936120.1750065301770.513550914013-47.565963032468.903585831114.3934216514
61.83970375493-0.146446924497-0.2589913764292.880183018910.667340677613.083006309590.00465737819869-0.429235918045-0.0645279122910.4838468513740.04947924956760.1719288731540.087266545730.057390223803-0.001568159501840.233768728816-0.0333495413613-0.01287457093060.3002222477980.113654376170.310459202229-53.74258652570.249629480622.8941350509
71.19263148968-1.581998129491.357631094573.35496008314-0.7432153135172.45978466227-0.1932831150980.05820466348510.6949436001560.2885615191510.0677225382022-0.502417927355-0.568892235597-0.1613527671190.07887911967080.2711858926220.1744474041840.06086691957560.335013383550.212775727720.494305779322-51.453566744275.17695866531.3188392001
82.63602124156-1.84556096315-1.84695559858.19966258412-0.156805449429.1611510446-0.3201745346910.255747830181-0.656756327790.06675830186840.510557649580.4152587980830.311943893787-0.992874120946-0.2848877479560.383734644115-0.155001179575-0.1050048982130.327744215620.09621208227350.336241146947-56.447923865641.845109578462.3100715099
90.0798621593661-0.147692237330.009285046561210.35476996774-0.131457775210.177894677949-0.1125324633010.01325468215820.1068733609340.318811511999-0.00660903848955-0.132709650382-0.1707106576040.0346490121116-0.1378561047670.1543305731720.0434130355143-0.0622126240038-0.1071793347840.2179496829790.411412873877-28.695227652850.799443167555.5939447035
100.07383194155930.167309032941-0.1934306463210.907436862336-0.4094168952860.496057811268-0.0569600792266-0.0526557253213-0.0037127556077-0.189914442349-0.156214773788-0.09098978267140.127020532591-0.0268321795579-1.040895960690.2755574460770.00118574952223-0.163797590548-0.0172407390960.1323985323910.364148651507-33.580350377446.431240474358.4734209582
110.368913479944-0.0138195498456-0.1990977733241.005877510880.2226463415750.488980585247-0.0238255042541-0.04411281115360.08374572581380.06036415078940.107941988012-0.23935494556-0.0252506744096-0.04819537313420.1519806298430.159977959737-0.03598450506850.03358066957760.127314628230.2151824257610.392210787063-39.067188098550.549723478254.9799084088
120.7464276140131.548774090960.3115130352456.385867206264.036158462063.714424085930.05427554112790.03819809740010.4046710900510.247022831994-0.0531705554645-0.047696692014-0.4016951851820.133958859127-0.09735701703130.27311323766-0.103384103242-0.1445518923460.1480828994960.1169288269520.365827642979-39.327381109266.133689028547.5405590665
132.04386467120.287945026261-0.9172816411390.7481893644210.35877216121.00309133298-0.05901028213140.29493060912-0.292046226107-0.204440340163-0.06853504937380.07687905779420.0622424719102-0.345759992949-0.242458645730.126940930811-0.0184639682379-0.07549952687550.2132454697010.1293377555370.390477230016-45.723204064354.271534204845.278480457
140.9150134543360.4609765456360.9284552267985.63574468413-0.1133542209011.005518830730.3408306513330.7154632167340.234864240613-0.795138046047-0.218627903417-0.763906067887-0.274142091042-0.1367891829140.0245519579190.4288939058290.08797953040770.02510801194160.3071629652130.139731573090.378568051065-39.323889570958.305751398235.2242165502
154.05884333236-1.2747704708-4.081896073295.719432396652.433396709625.41625362879-0.140423964893-0.5200796707980.08427188124620.753004934075-0.106455244040.0331117219251-0.01156466405770.464704674548-0.06854005964510.460146354479-0.0901931779629-0.07159459717590.259742492290.03581111251220.191497641585-37.503424119357.721312244872.8518777725
163.058852822290.760906085999-0.7062586272591.94551642933-0.3039968580322.669179244670.187617839721-0.02023524664180.2272843014940.498206908193-0.09557999568410.712121976284-0.193369122735-0.255525731886-0.1153574906520.376559948917-0.106253967390.06090331955360.2004750698180.04292271179250.451310331625-50.719414957674.483183555876.3544428789
171.6890123942-0.363016103714-0.7318313283650.793312685623-1.723423507685.25181883539-0.0209681238709-0.243450427275-0.06498879653940.2857148955910.03373066624080.376949255957-0.880943435982-0.368790868307-0.192470648430.7050654016080.06253271572380.265631054930.431126953170.08093770329960.426804685424-55.740334831867.544670422290.0058795742
182.30850174335-0.988768685446-0.389291105261.73991269519-1.177697142452.283982843960.0688392140199-0.212682543940.3229297625770.837343460821-0.0180183428641-0.127874560159-0.2503751305940.3051758753750.05287203058280.749099638846-0.09973767914740.03110705681760.2918949686140.09288577610580.36342008595-42.856811748876.820772502887.6200011825
194.80242406409-0.881309835579-2.584391017672.12935870657-0.7399825410914.910913963290.0218535704392-0.4758702545470.04739676868190.385911131786-0.0697831338434-0.3376445059950.1878222214190.3940172569640.03034985591930.642219276218-0.219497950279-0.09939214249460.3759723957660.02810877048690.211216671369-37.037524357175.378897707795.8447749689
204.400627767572.074099736883.671631844912.834830028550.8313377307743.500209462710.547079203804-1.479679500730.4819023672610.648321176175-0.1309593089180.4343041763850.4052058608470.0701107006506-0.3944239448341.20659424031-0.2742684240880.1825597697910.830945366008-0.1257896031770.425919543298-48.414332466177.3226274655100.9553578
212.00339756037-0.2118913707151.370157752231.81369697327-1.048264853342.808078023560.128912891330.139148531056-0.1172602120840.1538768467990.0346107074310.0468839604106-0.269065813467-0.3240529323030.5055805714690.2251109277450.14274434505-0.04649938632330.4341346902190.09392684811960.145957303391-49.532119502276.8099259877-7.91626964615
222.047983657081.554678301310.4521832461661.604925893931.842076018285.47594980657-0.01412365165940.181481413604-0.7748970359430.4495555960110.241622252908-0.527390663790.9240767533120.271394363617-0.3284221145610.217558961-0.0980379030917-0.06690033386120.391292725803-0.03781883055780.411006447541-37.755203878752.7897262395-14.3724240615
232.773116823050.4215354373290.1706792077381.75048669806-0.307476299331.48453842546-0.004663506124020.510688740671-0.40066379397-0.09100701169420.165299631163-0.2271868020540.12756194260.419539308874-0.120778084410.205396358988-0.0230886185156-0.01567818444980.479086541821-0.05113331062810.374616343578-33.902701829964.1281458297-18.0109888532
243.18492661367-1.889513141370.4656061091726.60956649508-0.2680984062354.30880811242-0.01178300536330.4358625675960.13129235851-0.743302164457-0.0840180956319-0.1392897495060.154495109903-0.1833945917620.1180283122570.26427021936-0.05254642963590.0017863641350.464503444883-0.05208741072330.241941579778-36.340816266767.0496687505-28.0955507908
253.71445319011-0.4131273331632.080273048145.341248598913.690598018794.07596669145-0.2977383588090.9861486871510.343143154485-1.103771456690.363634704723-0.727117264503-0.1885030116690.408853360966-0.1818128099420.756415119406-0.1985482704250.1427016130950.837925055487-0.03411587062860.333732019558-30.365832956766.0773131865-34.8815555268
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 29 through 42 )
2X-RAY DIFFRACTION2chain 'A' and (resid 43 through 66 )
3X-RAY DIFFRACTION3chain 'A' and (resid 67 through 95 )
4X-RAY DIFFRACTION4chain 'A' and (resid 96 through 110 )
5X-RAY DIFFRACTION5chain 'A' and (resid 111 through 142 )
6X-RAY DIFFRACTION6chain 'A' and (resid 143 through 203 )
7X-RAY DIFFRACTION7chain 'A' and (resid 204 through 217 )
8X-RAY DIFFRACTION8chain 'B' and (resid 31 through 42 )
9X-RAY DIFFRACTION9chain 'B' and (resid 43 through 56 )
10X-RAY DIFFRACTION10chain 'B' and (resid 57 through 66 )
11X-RAY DIFFRACTION11chain 'B' and (resid 67 through 110 )
12X-RAY DIFFRACTION12chain 'B' and (resid 111 through 125 )
13X-RAY DIFFRACTION13chain 'B' and (resid 126 through 187 )
14X-RAY DIFFRACTION14chain 'B' and (resid 188 through 217 )
15X-RAY DIFFRACTION15chain 'C' and (resid 32 through 42 )
16X-RAY DIFFRACTION16chain 'C' and (resid 43 through 95 )
17X-RAY DIFFRACTION17chain 'C' and (resid 96 through 110 )
18X-RAY DIFFRACTION18chain 'C' and (resid 111 through 172 )
19X-RAY DIFFRACTION19chain 'C' and (resid 173 through 199 )
20X-RAY DIFFRACTION20chain 'C' and (resid 200 through 217 )
21X-RAY DIFFRACTION21chain 'D' and (resid 31 through 42 )
22X-RAY DIFFRACTION22chain 'D' and (resid 43 through 66 )
23X-RAY DIFFRACTION23chain 'D' and (resid 67 through 142 )
24X-RAY DIFFRACTION24chain 'D' and (resid 143 through 187 )
25X-RAY DIFFRACTION25chain 'D' and (resid 188 through 217 )

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