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Yorodumi- PDB-6y7p: The complex between the eight-bladed symmetrical designer protein... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6y7p | ||||||
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Title | The complex between the eight-bladed symmetrical designer protein Tako8 and 1:2 zirconium(IV) Wells-Dawson (ZrWD) | ||||||
Components | Tako8 | ||||||
Keywords | DE NOVO PROTEIN / Designer protein / polyoxometalate / POM / ZrWD / hybrid / framework | ||||||
Function / homology | W-Zr-cluster Function and homology information | ||||||
Biological species | synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.75 Å | ||||||
Authors | Vandebroek, L. / Noguchi, H. / Parac-Vogt, T.N. / Van Meervelt, L. / Voet, A.R.D. | ||||||
Citation | Journal: Cryst.Growth Des. / Year: 2021 Title: Shape and Size Complementarity-Induced Formation of Supramolecular Protein Assemblies with Metal-Oxo Clusters Authors: Vandebroek, L. / Noguchi, H. / Kamata, K. / Tame, J.R.H. / Parac-Vogt, T.N. / Voet, A.R.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6y7p.cif.gz | 131.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6y7p.ent.gz | 82.4 KB | Display | PDB format |
PDBx/mmJSON format | 6y7p.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/y7/6y7p ftp://data.pdbj.org/pub/pdb/validation_reports/y7/6y7p | HTTPS FTP |
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-Related structure data
Related structure data | 6y7nC 6y7oC 6g6mS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 35024.230 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: Escherichia coli (E. coli) | ||||
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#2: Chemical | ChemComp-ZRW / #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.61 Å3/Da / Density % sol: 52.92 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / Details: 0.1M sodium acetate pH 4.5, 3.1M sodium chloride |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 2 / Wavelength: 1 Å |
Detector | Type: DECTRIS EIGER2 X 9M / Detector: PIXEL / Date: Jun 28, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→46.834 Å / Num. obs: 70938 / % possible obs: 99.8 % / Redundancy: 6.4 % / CC1/2: 0.996 / Rmerge(I) obs: 0.088 / Rpim(I) all: 0.058 / Rrim(I) all: 0.106 / Net I/σ(I): 10.8 |
Reflection shell | Resolution: 1.75→1.78 Å / Rmerge(I) obs: 0.729 / Mean I/σ(I) obs: 2.5 / Num. unique obs: 1977 / CC1/2: 0.81 / Rpim(I) all: 0.459 / Rrim(I) all: 0.863 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6g6m Resolution: 1.75→46.834 Å / SU ML: 0.24 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 23.63
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 57.94 Å2 / Biso mean: 19.338 Å2 / Biso min: 8.72 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.75→46.834 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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