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- PDB-6xhs: Crystal structure of S. aureus TarI in complex with CTP (space gr... -

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Basic information

Entry
Database: PDB / ID: 6xhs
TitleCrystal structure of S. aureus TarI in complex with CTP (space group P1211)
ComponentsRibitol-5-phosphate cytidylyltransferase 1
KeywordsTRANSFERASE / cytidylyltransferase
Function / homology
Function and homology information


D-ribitol-5-phosphate cytidylyltransferase / D-ribitol-5-phosphate cytidylyltransferase activity / poly(ribitol phosphate) teichoic acid biosynthetic process / 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity / isoprenoid biosynthetic process / cell wall organization
Similarity search - Function
Ribitol-5-phosphate cytidylyltransferase / 4-diphosphocytidyl-2C-methyl-D-erythritol synthase, conserved site / Cytidylyltransferase IspD/TarI / 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase / 4-diphosphocytidyl-2C-methyl-D-erythritol synthase signature. / Nucleotide-diphospho-sugar transferases
Similarity search - Domain/homology
CYTIDINE-5'-TRIPHOSPHATE / THIOCYANATE ION / Ribitol-5-phosphate cytidylyltransferase 1
Similarity search - Component
Biological speciesStaphylococcus aureus (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.9 Å
AuthorsLi, F.K.K. / Strynadka, N.C.J.
Funding support Canada, 3items
OrganizationGrant numberCountry
Canadian Institutes of Health Research (CIHR) Canada
Howard Hughes Medical Institute (HHMI) Canada
Natural Sciences and Engineering Research Council (NSERC, Canada) Canada
CitationJournal: J.Struct.Biol. / Year: 2021
Title: Crystallographic analysis of TarI and TarJ, a cytidylyltransferase and reductase pair for CDP-ribitol synthesis in Staphylococcus aureus wall teichoic acid biogenesis.
Authors: Li, F.K.K. / Gale, R.T. / Petrotchenko, E.V. / Borchers, C.H. / Brown, E.D. / Strynadka, N.C.J.
History
DepositionJun 19, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 21, 2021Provider: repository / Type: Initial release
Revision 1.1Apr 28, 2021Group: Database references / Category: citation / Item: _citation.journal_volume
Revision 1.2Oct 18, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Ribitol-5-phosphate cytidylyltransferase 1
B: Ribitol-5-phosphate cytidylyltransferase 1
C: Ribitol-5-phosphate cytidylyltransferase 1
D: Ribitol-5-phosphate cytidylyltransferase 1
E: Ribitol-5-phosphate cytidylyltransferase 1
F: Ribitol-5-phosphate cytidylyltransferase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)175,90011
Polymers174,7936
Non-polymers1,1075
Water18010
1
A: Ribitol-5-phosphate cytidylyltransferase 1
B: Ribitol-5-phosphate cytidylyltransferase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)58,7483
Polymers58,2642
Non-polymers4831
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4380 Å2
ΔGint-22 kcal/mol
Surface area19520 Å2
MethodPISA
2
C: Ribitol-5-phosphate cytidylyltransferase 1
D: Ribitol-5-phosphate cytidylyltransferase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)58,3233
Polymers58,2642
Non-polymers581
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3720 Å2
ΔGint-25 kcal/mol
Surface area20130 Å2
MethodPISA
3
E: Ribitol-5-phosphate cytidylyltransferase 1
F: Ribitol-5-phosphate cytidylyltransferase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)58,8305
Polymers58,2642
Non-polymers5663
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4590 Å2
ΔGint-39 kcal/mol
Surface area19400 Å2
MethodPISA
Unit cell
Length a, b, c (Å)49.357, 138.521, 109.564
Angle α, β, γ (deg.)90.000, 96.460, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 1 through 11 or (resid 20...
21(chain B and (resid 1 or (resid 2 and (name...
31(chain C and (resid 1 through 11 or (resid 20...
41(chain D and (resid 1 or (resid 2 and (name...
51(chain E and (resid 1 through 11 or (resid 20...
61(chain F and (resid 1 through 11 or (resid 20...

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11METMETILEILE(chain A and (resid 1 through 11 or (resid 20...AA1 - 111 - 11
12LEULEULEULEU(chain A and (resid 1 through 11 or (resid 20...AA2020
13METMETGLYGLY(chain A and (resid 1 through 11 or (resid 20...AA1 - 2341 - 234
14METMETGLYGLY(chain A and (resid 1 through 11 or (resid 20...AA1 - 2341 - 234
15METMETGLYGLY(chain A and (resid 1 through 11 or (resid 20...AA1 - 2341 - 234
16METMETGLYGLY(chain A and (resid 1 through 11 or (resid 20...AA1 - 2341 - 234
21METMETMETMET(chain B and (resid 1 or (resid 2 and (name...BB11
22LYSLYSLYSLYS(chain B and (resid 1 or (resid 2 and (name...BB22
23METMETILEILE(chain B and (resid 1 or (resid 2 and (name...BB1 - 2351 - 235
24METMETILEILE(chain B and (resid 1 or (resid 2 and (name...BB1 - 2351 - 235
25METMETILEILE(chain B and (resid 1 or (resid 2 and (name...BB1 - 2351 - 235
26METMETILEILE(chain B and (resid 1 or (resid 2 and (name...BB1 - 2351 - 235
31METMETILEILE(chain C and (resid 1 through 11 or (resid 20...CC1 - 111 - 11
32LEULEULEULEU(chain C and (resid 1 through 11 or (resid 20...CC2020
33METMETALAALA(chain C and (resid 1 through 11 or (resid 20...CC1 - 2361 - 236
34METMETALAALA(chain C and (resid 1 through 11 or (resid 20...CC1 - 2361 - 236
35METMETALAALA(chain C and (resid 1 through 11 or (resid 20...CC1 - 2361 - 236
41METMETMETMET(chain D and (resid 1 or (resid 2 and (name...DD11
42LYSLYSLYSLYS(chain D and (resid 1 or (resid 2 and (name...DD22
43METMETILEILE(chain D and (resid 1 or (resid 2 and (name...DD1 - 2351 - 235
44METMETILEILE(chain D and (resid 1 or (resid 2 and (name...DD1 - 2351 - 235
45METMETILEILE(chain D and (resid 1 or (resid 2 and (name...DD1 - 2351 - 235
46METMETILEILE(chain D and (resid 1 or (resid 2 and (name...DD1 - 2351 - 235
51METMETILEILE(chain E and (resid 1 through 11 or (resid 20...EE1 - 111 - 11
52LEULEULEULEU(chain E and (resid 1 through 11 or (resid 20...EE2020
53METMETARGARG(chain E and (resid 1 through 11 or (resid 20...EE1 - 2321 - 232
61METMETILEILE(chain F and (resid 1 through 11 or (resid 20...FF1 - 111 - 11
62LEULEULEULEU(chain F and (resid 1 through 11 or (resid 20...FF2020
63METMETILEILE(chain F and (resid 1 through 11 or (resid 20...FF1 - 2351 - 235

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Components

#1: Protein
Ribitol-5-phosphate cytidylyltransferase 1


Mass: 29132.209 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: tarI, SAOUHSC_00225 / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: Q2G1C0, D-ribitol-5-phosphate cytidylyltransferase
#2: Chemical ChemComp-CTP / CYTIDINE-5'-TRIPHOSPHATE


Mass: 483.156 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C9H16N3O14P3 / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical ChemComp-SCN / THIOCYANATE ION


Mass: 58.082 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: CNS
#4: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 10 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.13 Å3/Da / Density % sol: 42.23 %
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop
Details: 0.25 M sodium thiocyanate, 22.5% PEG3350, 20 mM CTP, 20 mM magnesium chloride

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: CLSI / Beamline: 08ID-1 / Wavelength: 0.97949 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 10, 2018
RadiationMonochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97949 Å / Relative weight: 1
ReflectionResolution: 2.9→49.044 Å / Num. obs: 32332 / % possible obs: 99.65 % / Redundancy: 3.4 % / CC1/2: 0.989 / Rpim(I) all: 0.1079 / Net I/σ(I): 6.08
Reflection shellResolution: 2.9→3.004 Å / Redundancy: 3.5 % / Mean I/σ(I) obs: 1.35 / Num. unique obs: 3173 / CC1/2: 0.539 / Rpim(I) all: 0.5925 / % possible all: 99.81

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Processing

Software
NameVersionClassification
PHENIX1.16_3549refinement
PDB_EXTRACT3.25data extraction
XDSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 4JIS

4jis


Resolution: 2.9→49.044 Å / SU ML: 0.44 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 29.63 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2669 1617 5 %
Rwork0.2099 30694 -
obs0.2132 32311 99.67 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 160.21 Å2 / Biso mean: 60.94 Å2 / Biso min: 12.11 Å2
Refinement stepCycle: final / Resolution: 2.9→49.044 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10463 0 94 10 10567
Biso mean--61.66 27.58 -
Num. residues----1394
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A3906X-RAY DIFFRACTION6.085TORSIONAL
12B3906X-RAY DIFFRACTION6.085TORSIONAL
13C3906X-RAY DIFFRACTION6.085TORSIONAL
14D3906X-RAY DIFFRACTION6.085TORSIONAL
15E3906X-RAY DIFFRACTION6.085TORSIONAL
16F3906X-RAY DIFFRACTION6.085TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.9-2.98540.39631320.32572505100
2.9854-3.08170.35921360.31322570100
3.0817-3.19180.37781330.29792529100
3.1918-3.31960.3251350.27312575100
3.3196-3.47060.30221340.25572543100
3.4706-3.65360.30011350.23112565100
3.6536-3.88240.29141340.21282547100
3.8824-4.1820.22871360.18342571100
4.182-4.60260.23491340.159254599
4.6026-5.26790.20951340.15962555100
5.2679-6.63440.26271360.21282577100
6.6344-49.0440.2181380.173261299
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.8441-2.12891.54583.2349-2.47868.778-0.3679-0.26560.54440.20880.1592-0.3871-0.06060.22720.16030.3041-0.00220.03370.2558-0.03140.45996.7913-0.285535.002
23.1433-0.6520.68152.7702-0.35153.6834-0.12120.04380.2611-0.0088-0.0189-0.0749-0.61470.08610.14970.3286-0.1148-0.00130.2834-0.01130.28532.62086.800827.5774
37.3859-4.3157-3.21493.9049-0.58536.19610.29320.8438-0.148-1.013-0.50320.1749-0.48491.23790.30530.7341-0.09150.01030.530.00460.31782.1764-2.287312.2275
40.7429-0.521-0.26655.82-1.77420.4136-0.1391-0.2932-0.3252-0.32970.1080.2579-0.07870.1244-0.00170.39780.00760.0040.3690.03610.3553-2.544-15.956624.1595
52.05182.4754-1.00029.4282.39822.4613-0.1086-0.55540.34860.6523-0.48540.99681.2444-0.50430.43770.4625-0.0759-0.03990.62150.09790.6648-13.7858-8.142630.6988
66.8383-0.86792.97754.16050.87731.953-0.20870.0747-0.268-0.26810.2476-0.1974-0.10760.1568-0.04160.4468-0.04650.03860.3230.10270.29156.1027-9.820924.8843
77.27571.1476-2.41668.4662-0.79234.24320.0389-0.3130.2888-0.3807-0.2512-0.8808-0.10080.56430.28260.22520.12860.11020.56920.01570.480524.2735-35.459930.3514
81.67361.1516-0.36164.22431.07472.2458-0.19921.0428-0.47580.07790.6394-1.0898-0.5588-0.223-0.44450.32110.08870.08340.7149-0.02150.876729.3461-40.289225.2054
90.9198-0.4621-0.5695.6583.03654.5673-0.0621-0.1549-0.08410.1623-0.0168-0.25310.4409-0.03490.07320.2711-0.01660.05920.3220.05730.360914.0765-40.542633.5987
102.9317-3.45333.54654.3393-5.5287.6468-0.26290.12090.2769-0.1972-0.0626-0.1337-0.46910.12780.33590.4063-0.0299-0.01950.35450.01370.40250.5965-22.06726.9364
112.4749-0.81232.02198.96263.23666.178-0.11960.7001-0.41880.13730.1241.6266-0.74960.01620.09050.4308-0.02390.18410.4386-0.01220.46126.0944-45.036229.833
128.12310.6879-5.99020.0885-0.84017.20470.00920.81060.8706-0.50380.04410.5758-0.3838-1.0306-0.18510.88190.0976-0.01810.44640.0920.3641.3164-39.37321.7855
135.03790.4289-1.06613.6524-2.46423.54660.07790.18230.98150.108-0.0839-0.16990.7176-0.04380.00270.05140.0641-0.02180.30950.02810.479714.8358-23.990831.4103
144.89821.3014-3.01565.5821.14947.2862-0.3542-0.2789-0.33420.30190.1808-0.41720.31710.38880.15730.3840.1186-0.01470.3017-0.04210.307810.5466-40.57962.3567
155.9121-1.0438-1.71165.71750.63386.6587-0.4833-0.0912-0.30130.77210.5254-0.20880.15-0.059-0.10440.44670.05320.01370.21150.010.26247.0211-48.378559.4597
160.8087-0.5309-0.7193.4221.69370.83560.0329-0.5312-0.17021.5020.20150.11480.3432-0.096-0.11770.70170.050.07460.45210.17230.41292.0617-40.230173.4074
173.1582-1.10041.89220.56690.50257.1542-0.5996-0.07420.50910.0246-0.1148-0.1316-0.37770.65120.55670.48380.03360.020.34120.05730.4767-2.6755-21.339966.018
185.896-0.4554-2.06668.3572-0.64596.0313-1.62290.1290.7016-0.06360.04210.61070.1338-0.69261.68320.73540.0469-0.30080.3277-0.10311.0111-7.648-13.619261.5488
196.91711.441.12917.99310.94774.55720.6269-0.1267-0.35991.19120.17620.91520.4226-0.3849-0.67830.34910.04410.13290.39410.19590.5814-5.5761-37.798968.0964
202.43321.3862.06055.5648-2.90655.90320.34392.09810.57860.14550.14440.3819-1.05990.2371-0.55150.5267-0.14910.00580.90870.04760.7265-12.0075-35.774860.1304
216.6985-0.33380.00474.8157-0.79243.713-0.3737-0.2970.50460.32590.42790.0472-0.6491-0.243-0.14390.57960.00810.01440.20930.02060.30991.5688-29.608269.8493
227.0006-2.2653-0.36444.858-5.40138.9906-0.9463-0.9110.832-1.0623-0.8282-1.29040.5711.69521.18170.47-0.02070.18780.5815-0.01220.779718.6988-25.428964.7548
234.0615-1.09721.66225.36810.0037.06690.107-0.00950.1286-0.0217-0.1498-0.3792-0.42460.32850.05180.4881-0.04840.0290.2989-0.0380.291812.6251-0.293467.3879
244.9837-0.6105-0.52131.5981.31734.4509-0.5854-0.50370.41370.55850.4061-0.0125-0.12590.19490.1770.8574-0.00470.03720.3582-0.0810.384915.15667.344271.4182
253.6255-1.51541.8985.2211-1.2824.4332-0.1167-0.13420.29630.4150.1369-0.1858-0.2461-0.65640.03130.7259-0.00610.05780.3553-0.00270.33273.80493.292958.7471
262.30122.7-3.72095.8506-5.2665.9196-0.1062-0.19080.03840.62510.01650.11880.04690.0890.09770.51830.00990.01780.3320.03370.4373-3.692-19.210563.2424
274.03772.2096-1.4825.9547-2.44022.0703-0.1515-0.19240.38290.84510.88351.1994-0.6519-1.2343-0.75710.70.14360.03920.65550.14840.6572-8.08951.590963.8438
284.42020.49792.28382.2638-1.91566.56930.091-0.4302-0.73470.28130.2640.20330.2748-0.3372-0.1890.54380.02270.01560.24250.06820.36092.9535-9.872658.7084
296.52831.8641-2.60335.6674-5.67586.4555-1.53490.5438-0.56020.11130.3052-1.27-0.3478-0.52631.20210.9313-0.04560.12730.3469-0.13510.662919.189-16.163365.9272
307.6673-1.93053.50594.42611.23612.6232-1.1676-0.0923-0.90370.53770.7384-0.5318-0.6918-0.86090.33810.4889-0.0430.09670.4971-0.12640.349517.7142-6.208108.5997
317.3711-2.67122.28249.2297-2.89079.6986-0.56380.2107-0.4050.95310.4662-0.6996-0.18970.56870.12060.49860.0737-0.12250.4373-0.13460.564524.6831-10.3953102.5851
324.5121-2.10921.06324.4626-2.11722.0812-0.23210.2554-0.05120.23850.1205-0.8782-1.29641.280.27730.6289-0.1922-0.04890.5080.00070.575325.0241-0.0684103.6211
334.8681-2.1475-0.74463.8828-1.13883.57150.2836-0.51260.2434-0.2666-0.64680.0626-0.95460.87330.28290.6127-0.1568-0.19220.62250.01730.475624.29342.0162103.1327
343.3384.0596-0.89855.913-0.72474.52730.2041-0.60440.95131.28450.08820.208-2.3197-0.6124-0.21081.02410.0682-0.24930.48060.00160.61312.57457.2597104.7715
355.65091.07331.85919.3468-2.0778.9294-0.82050.03460.70091.06920.5933-0.3157-1.5204-0.83040.22460.4469-0.0034-0.08680.5047-0.01880.311714.025-0.377896.4938
364.03751.628-3.79285.863-0.11238.10880.06710.16130.3989-1.62920.40110.3070.33080.1915-0.39360.6937-0.1273-0.11510.520.06360.450810.3292-3.498787.3821
373.99354.6249-3.65627.9003-4.50213.26380.1755-0.63160.38751.89020.03090.6605-0.90210.2989-0.34450.43280.00480.03980.5280.13790.4722-0.4529-15.60799.718
386.68820.57311.0110.33361.07354.24890.81671.38940.74211.31180.92951.78530.6304-0.7824-1.00861.00910.04740.20360.32920.42770.8675-3.8761-17.3289101.2656
397.9737-0.62531.3834.1162-4.10864.17480.43310.4070.55681.00130.34780.6784-1.3755-0.3043-0.17270.60770.0356-0.0690.62040.14060.60524.53466.094198.7065
408.3603-0.2967-4.50216.70751.71582.923-0.1998-3.58140.10291.70720.2170.41010.34010.4023-0.0251.32710.209-0.01331.83920.12120.7335-1.2240.7782108.4013
415.06151.74513.44683.8781-1.42748.1747-0.00880.13650.4999-0.17320.30451.5742-0.8332-0.8638-0.140.53460.1079-0.01120.61160.27340.6699-1.001-2.199595.6952
429.3775-5.50964.51445.6222-2.72359.59470.2904-0.1175-1.72750.6723-0.1125-0.177-0.17140.5663-0.21290.535-0.1007-0.11650.40180.09360.769616.3491-19.7646102.2698
433.9389-1.10970.02882.8050.37713.2862-0.1780.01880.05170.23190.131-0.3714-0.1834-0.12820.0640.6861-0.0043-0.03520.37240.01340.45462.2959-43.333197.3219
449.19452.66011.84446.07660.16871.543-0.20040.1072-0.3517-0.3350.17140.2570.3627-0.11050.08360.9728-0.0430.09440.4641-0.02330.4078-5.8751-44.5433102.5139
452.11081.14431.84333.6704-1.35522.70580.35520.155-0.09761.1312-0.33160.1599-0.2320.38980.06050.91160.05890.19090.50530.00720.4202-3.0645-22.9743107.0436
465.60532.03320.17076.5351.15364.01260.2598-0.3122-0.16670.50840.13890.447-0.2202-0.4238-0.33920.57680.04840.05690.40890.15030.4066-3.8596-31.4317103.7992
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 31 )A1 - 31
2X-RAY DIFFRACTION2chain 'A' and (resid 32 through 118 )A32 - 118
3X-RAY DIFFRACTION3chain 'A' and (resid 119 through 132 )A119 - 132
4X-RAY DIFFRACTION4chain 'A' and (resid 133 through 182 )A133 - 182
5X-RAY DIFFRACTION5chain 'A' and (resid 183 through 195 )A183 - 195
6X-RAY DIFFRACTION6chain 'A' and (resid 196 through 234 )A196 - 234
7X-RAY DIFFRACTION7chain 'B' and (resid 1 through 54 )B1 - 54
8X-RAY DIFFRACTION8chain 'B' and (resid 55 through 83 )B55 - 83
9X-RAY DIFFRACTION9chain 'B' and (resid 84 through 132 )B84 - 132
10X-RAY DIFFRACTION10chain 'B' and (resid 133 through 169 )B133 - 169
11X-RAY DIFFRACTION11chain 'B' and (resid 170 through 182 )B170 - 182
12X-RAY DIFFRACTION12chain 'B' and (resid 183 through 201 )B183 - 201
13X-RAY DIFFRACTION13chain 'B' and (resid 202 through 235 )B202 - 235
14X-RAY DIFFRACTION14chain 'C' and (resid 1 through 54 )C1 - 54
15X-RAY DIFFRACTION15chain 'C' and (resid 55 through 98 )C55 - 98
16X-RAY DIFFRACTION16chain 'C' and (resid 99 through 132 )C99 - 132
17X-RAY DIFFRACTION17chain 'C' and (resid 133 through 150 )C133 - 150
18X-RAY DIFFRACTION18chain 'C' and (resid 151 through 163 )C151 - 163
19X-RAY DIFFRACTION19chain 'C' and (resid 164 through 182 )C164 - 182
20X-RAY DIFFRACTION20chain 'C' and (resid 183 through 201 )C183 - 201
21X-RAY DIFFRACTION21chain 'C' and (resid 202 through 220 )C202 - 220
22X-RAY DIFFRACTION22chain 'C' and (resid 221 through 236 )C221 - 236
23X-RAY DIFFRACTION23chain 'D' and (resid 1 through 54 )D1 - 54
24X-RAY DIFFRACTION24chain 'D' and (resid 55 through 83 )D55 - 83
25X-RAY DIFFRACTION25chain 'D' and (resid 84 through 132 )D84 - 132
26X-RAY DIFFRACTION26chain 'D' and (resid 133 through 169 )D133 - 169
27X-RAY DIFFRACTION27chain 'D' and (resid 170 through 201 )D170 - 201
28X-RAY DIFFRACTION28chain 'D' and (resid 202 through 220 )D202 - 220
29X-RAY DIFFRACTION29chain 'D' and (resid 221 through 235 )D221 - 235
30X-RAY DIFFRACTION30chain 'E' and (resid 1 through 21 )E1 - 21
31X-RAY DIFFRACTION31chain 'E' and (resid 22 through 41 )E22 - 41
32X-RAY DIFFRACTION32chain 'E' and (resid 42 through 67 )E42 - 67
33X-RAY DIFFRACTION33chain 'E' and (resid 68 through 83 )E68 - 83
34X-RAY DIFFRACTION34chain 'E' and (resid 84 through 98 )E84 - 98
35X-RAY DIFFRACTION35chain 'E' and (resid 99 through 118 )E99 - 118
36X-RAY DIFFRACTION36chain 'E' and (resid 119 through 132 )E119 - 132
37X-RAY DIFFRACTION37chain 'E' and (resid 133 through 150 )E133 - 150
38X-RAY DIFFRACTION38chain 'E' and (resid 151 through 169 )E151 - 169
39X-RAY DIFFRACTION39chain 'E' and (resid 170 through 182 )E170 - 182
40X-RAY DIFFRACTION40chain 'E' and (resid 183 through 195 )E183 - 195
41X-RAY DIFFRACTION41chain 'E' and (resid 196 through 210 )E196 - 210
42X-RAY DIFFRACTION42chain 'E' and (resid 211 through 232 )E211 - 232
43X-RAY DIFFRACTION43chain 'F' and (resid 1 through 67 )F1 - 67
44X-RAY DIFFRACTION44chain 'F' and (resid 68 through 118 )F68 - 118
45X-RAY DIFFRACTION45chain 'F' and (resid 119 through 169 )F119 - 169
46X-RAY DIFFRACTION46chain 'F' and (resid 170 through 235 )F170 - 235

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