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- PDB-6xf9: Crystal structure of KSHV ORF68 -

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Basic information

Entry
Database: PDB / ID: 6xf9
TitleCrystal structure of KSHV ORF68
ComponentsPackaging protein UL32
KeywordsVIRAL PROTEIN / viral packaging / viral cleavage
Function / homologyHerpesvirus major envelope glycoprotein / Herpesvirus putative major envelope glycoprotein / host cell cytoplasm / viral envelope / host cell nucleus / cytoplasm / Packaging protein UL32
Function and homology information
Biological speciesHuman herpesvirus 8 type M
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.22 Å
AuthorsDidychuk, A.L. / Gates, S.N. / Martin, A. / Glaunsinger, B.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI122528 United States
Howard Hughes Medical Institute (HHMI) United States
CitationJournal: Elife / Year: 2021
Title: A pentameric protein ring with novel architecture is required for herpesviral packaging.
Authors: Allison L Didychuk / Stephanie N Gates / Matthew R Gardner / Lisa M Strong / Andreas Martin / Britt A Glaunsinger /
Abstract: Genome packaging in large double-stranded DNA viruses requires a powerful molecular motor to force the viral genome into nascent capsids, which involves essential accessory factors that are poorly ...Genome packaging in large double-stranded DNA viruses requires a powerful molecular motor to force the viral genome into nascent capsids, which involves essential accessory factors that are poorly understood. Here, we present structures of two such accessory factors from the oncogenic herpesviruses Kaposi's sarcoma-associated herpesvirus (KSHV; ORF68) and Epstein-Barr virus (EBV; BFLF1). These homologous proteins form highly similar homopentameric rings with a positively charged central channel that binds double-stranded DNA. Mutation of individual positively charged residues within but not outside the channel ablates DNA binding, and in the context of KSHV infection, these mutants fail to package the viral genome or produce progeny virions. Thus, we propose a model in which ORF68 facilitates the transfer of newly replicated viral genomes to the packaging motor.
History
DepositionJun 15, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 24, 2021Provider: repository / Type: Initial release
Revision 1.1Mar 6, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.2Apr 3, 2024Group: Refinement description / Category: pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Packaging protein UL32
B: Packaging protein UL32
C: Packaging protein UL32
D: Packaging protein UL32
E: Packaging protein UL32
hetero molecules


Theoretical massNumber of molelcules
Total (without water)260,82120
Polymers259,8405
Non-polymers98115
Water543
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)134.987, 224.042, 192.270
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number20
Space group name H-MC2221
Space group name HallC2c2
Symmetry operation#1: x,y,z
#2: x,-y,-z
#3: -x,y,-z+1/2
#4: -x,-y,z+1/2
#5: x+1/2,y+1/2,z
#6: x+1/2,-y+1/2,-z
#7: -x+1/2,y+1/2,-z+1/2
#8: -x+1/2,-y+1/2,z+1/2
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 2 through 15 or (resid 16...
d_2ens_1(chain "B" and (resid 2 through 68 or resid 70...
d_3ens_1(chain "C" and (resid 2 through 30 or (resid 31...
d_4ens_1(chain "D" and (resid 2 through 30 or (resid 31...
d_5ens_1(chain "E" and (resid 2 through 30 or (resid 31...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1PHEPROA1 - 63
d_12ens_1GLYPROA65 - 81
d_13ens_1CYSPROA85 - 105
d_14ens_1GLUGLUA107
d_15ens_1ALATRPA109 - 169
d_16ens_1CYSCYSA173 - 174
d_17ens_1ILETHRA176 - 179
d_18ens_1THRTHRA181
d_19ens_1PROPROA183 - 188
d_110ens_1LEUALAA190 - 191
d_111ens_1ALALEUA193 - 221
d_112ens_1PROPROA223 - 269
d_113ens_1ALAARGA271 - 350
d_114ens_1GLNLYSA352 - 355
d_115ens_1LEUGLYA357 - 394
d_116ens_1THRILEA396 - 402
d_117ens_1GLULEUA404 - 409
d_118ens_1ARGALAA411 - 427
d_21ens_1PHEPROB1 - 63
d_22ens_1GLYPROB65 - 81
d_23ens_1CYSPROB85 - 105
d_24ens_1GLUGLUB107
d_25ens_1ALAILEB109 - 164
d_26ens_1PHETRPB166 - 170
d_27ens_1CYSCYSB172 - 173
d_28ens_1ILETHRB175 - 178
d_29ens_1THRTHRB180
d_210ens_1PROPROB182 - 187
d_211ens_1LEUALAB189 - 190
d_212ens_1ALALEUB192 - 220
d_213ens_1PROPROB222 - 268
d_214ens_1ALAARGB270 - 349
d_215ens_1GLNLYSB351 - 354
d_216ens_1LEUGLYB356 - 393
d_217ens_1THRILEB395 - 401
d_218ens_1GLULEUB403 - 408
d_219ens_1ARGALAB410 - 426
d_31ens_1PHEPROC1 - 63
d_32ens_1GLYPROC65 - 81
d_33ens_1CYSPROC84 - 104
d_34ens_1GLUGLUC106
d_35ens_1ALAILEC108 - 163
d_36ens_1PHETRPC165 - 169
d_37ens_1CYSCYSC172 - 173
d_38ens_1ILETHRC175 - 178
d_39ens_1THRTHRC180
d_310ens_1PROPROC182 - 187
d_311ens_1LEUALAC189 - 190
d_312ens_1ALAPROC192 - 267
d_313ens_1ALAARGC269 - 348
d_314ens_1GLNLYSC350 - 353
d_315ens_1LEUGLYC355 - 392
d_316ens_1THRILEC394 - 400
d_317ens_1GLULEUC402 - 407
d_318ens_1ARGALAC409 - 425
d_41ens_1PHEPROD1 - 63
d_42ens_1GLYPROD65 - 81
d_43ens_1CYSPROD85 - 105
d_44ens_1GLUGLUD107
d_45ens_1ALAILED109 - 164
d_46ens_1PHETRPD166 - 170
d_47ens_1CYSCYSD174 - 175
d_48ens_1ILETHRD177 - 180
d_49ens_1THRTHRD182
d_410ens_1PROPROD184 - 189
d_411ens_1LEUALAD191 - 192
d_412ens_1ALALEUD194 - 222
d_413ens_1PROPROD224 - 270
d_414ens_1ALAARGD272 - 351
d_415ens_1GLNLYSD353 - 356
d_416ens_1LEUGLYD358 - 395
d_417ens_1THRILED397 - 403
d_418ens_1GLULEUD405 - 410
d_419ens_1ARGALAD412 - 428
d_51ens_1PHEPROE1 - 63
d_52ens_1GLYPROE65 - 81
d_53ens_1CYSPROE83 - 103
d_54ens_1GLUGLUE105
d_55ens_1ALAILEE107 - 162
d_56ens_1PHETRPE164 - 168
d_57ens_1CYSCYSE171 - 172
d_58ens_1ILETHRE174 - 177
d_59ens_1THRTHRE179
d_510ens_1PROPROE181 - 186
d_511ens_1LEUALAE188 - 189
d_512ens_1ALALEUE191 - 219
d_513ens_1PROPROE221 - 267
d_514ens_1ALAARGE269 - 348
d_515ens_1GLNLYSE350 - 353
d_516ens_1LEUGLYE355 - 392
d_517ens_1THRILEE394 - 400
d_518ens_1GLULEUE402 - 407
d_519ens_1ARGALAE409 - 425

NCS oper:
IDCodeMatrixVector
1given(0.319696500035, 0.947421894461, -0.0136345796112), (-0.947249891014, 0.319915840701, 0.0192743052633), (0.0226228168059, 0.00675342611747, 0.99972126085)-109.840137153, -17.3133169346, 1.54260402811
2given(-0.808430505086, 0.588574546283, -0.00448574568269), (-0.588553786628, -0.808268605692, 0.0175014656293), (0.00667522978361, 0.0167888213058, 0.999836775072)-161.755225666, 82.26642366, -0.277724785901
3given(-0.809492156889, -0.587041995264, -0.0102050836258), (0.587097065669, -0.809509535331, -0.00336863342807), (-0.00628358321505, -0.0087182569911, 0.999942252621)-82.9812489198, 160.966826163, -0.00720836811348
4given(0.303583603352, -0.952802231016, -0.0022145757771), (0.952706911936, 0.303584909577, -0.0136287426866), (0.0136578082247, 0.0020276211641, 0.99990467197)16.7416567304, 110.447460519, 1.1520118631

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Components

#1: Protein
Packaging protein UL32


Mass: 51967.977 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human herpesvirus 8 type M / Production host: Homo sapiens (human) / References: UniProt: P88958
#2: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 15 / Source method: obtained synthetically / Formula: Zn
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.8 Å3/Da / Density % sol: 56.03 %
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.5
Details: 310 mM CaCl2, 95 mM HEPES pH 7.5, 26.6% PEG-400, 5% glycerol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 8.3.1 / Wavelength: 1.11589 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Sep 28, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.11589 Å / Relative weight: 1
ReflectionResolution: 2.22→49.543 Å / Num. obs: 189934 / % possible obs: 95.3 % / Redundancy: 13.3 % / Biso Wilson estimate: 54.83 Å2 / CC1/2: 0.999 / Net I/σ(I): 19.4
Reflection shellResolution: 2.22→2.48 Å / Mean I/σ(I) obs: 1.7 / Num. unique obs: 4570 / CC1/2: 0.565

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Processing

Software
NameVersionClassification
PHENIX1.18.1_3865refinement
Cootmodel building
XDSdata reduction
STARANISOdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: ORF68-polyA

Resolution: 2.22→49.54 Å / SU ML: 0.3051 / Cross valid method: FREE R-VALUE / σ(F): 1.91 / Phase error: 37.8445
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2479 3866 2.19 %
Rwork0.2221 173023 -
obs0.2227 176889 63.37 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 62.79 Å2
Refinement stepCycle: LAST / Resolution: 2.22→49.54 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms16342 0 15 3 16360
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.013416774
X-RAY DIFFRACTIONf_angle_d1.379922826
X-RAY DIFFRACTIONf_chiral_restr0.07482666
X-RAY DIFFRACTIONf_plane_restr0.012910
X-RAY DIFFRACTIONf_dihedral_angle_d17.8576072
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS0.624954067273
ens_1d_3AX-RAY DIFFRACTIONTorsion NCS0.519494272197
ens_1d_4AX-RAY DIFFRACTIONTorsion NCS0.600694485085
ens_1d_5AX-RAY DIFFRACTIONTorsion NCS0.581131574385
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.22-2.24000.353839X-RAY DIFFRACTION0.4
2.24-2.270.331980.4162149X-RAY DIFFRACTION1.59
2.27-2.30.3678100.3657284X-RAY DIFFRACTION2.95
2.3-2.330.305980.3439676X-RAY DIFFRACTION6.9
2.33-2.370.2928370.35911361X-RAY DIFFRACTION14.02
2.37-2.40.4146390.35081791X-RAY DIFFRACTION18.47
2.4-2.440.3276390.35072070X-RAY DIFFRACTION21.22
2.44-2.480.2874610.35282278X-RAY DIFFRACTION23.54
2.48-2.520.3172670.3422690X-RAY DIFFRACTION27.52
2.52-2.570.3836600.343228X-RAY DIFFRACTION32.95
2.57-2.620.3267850.34113995X-RAY DIFFRACTION41.05
2.62-2.670.3481040.34195262X-RAY DIFFRACTION53.57
2.67-2.730.31041560.35126514X-RAY DIFFRACTION67.2
2.73-2.790.32021780.33157691X-RAY DIFFRACTION79.36
2.79-2.860.35772020.32438581X-RAY DIFFRACTION87.83
2.86-2.940.37321990.3159396X-RAY DIFFRACTION96.41
2.94-3.030.33532190.31049759X-RAY DIFFRACTION100
3.03-3.130.29692240.29989754X-RAY DIFFRACTION100
3.13-3.240.3482130.28389742X-RAY DIFFRACTION99.99
3.24-3.370.29192090.25019785X-RAY DIFFRACTION100
3.37-3.520.2722130.23759746X-RAY DIFFRACTION100
3.52-3.710.26322160.20699729X-RAY DIFFRACTION99.99
3.71-3.940.20732260.19629766X-RAY DIFFRACTION100
3.94-4.240.20252160.17489743X-RAY DIFFRACTION100
4.24-4.670.19872220.17619771X-RAY DIFFRACTION100
4.67-5.340.20942140.17469734X-RAY DIFFRACTION99.99
5.34-6.730.23452240.2159747X-RAY DIFFRACTION100
6.73-49.540.20382170.17589742X-RAY DIFFRACTION99.74
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.46984249296-1.37006904085-0.8803193461811.80781810377-0.5205011786281.655776101820.05988710509570.355696386308-0.0128216509583-0.2223376072190.00492265801946-0.127600667291-0.4123646311570.0547833850447-0.1037329793610.62493847902-0.1201473744890.04190234451760.384100953566-0.05027685232630.206804926226-49.4314350066108.239387759-36.983311225
24.21979433002-2.03060714085-1.774440770174.686304317041.759739327514.913375005440.145176726169-0.3305326502170.3034930460930.413767052380.137172705852-0.634358212024-0.6163097533890.973907152665-0.2643635863610.736824103225-0.149548861712-0.04424313014230.42396214085-0.05768886577310.327034901017-42.5902903488110.365873949-20.9057185054
31.57693267161-1.38507758633-0.1650105796454.27072019301-0.09240457656353.425996667180.2023325490950.05099999936160.270250781010.061125705773-0.0102810843679-0.50369044937-0.8207392262550.771476400686-0.1843489924460.695271394381-0.164966807570.0162766490970.402615938313-0.0653624230520.328091435542-44.3090208811110.832998515-27.6243250716
42.45590474545-0.88481007862-1.153114741023.51507610265-1.744224001225.292270403670.141855077621-0.0240169840611-0.3204979666320.4005302592430.0400069057110.03897401724570.2474868918010.0197154080174-0.2016599715640.55933896123-0.0738108438033-0.04601226048510.255372714883-0.04833627386090.367887105596-53.267029339290.7475996587-19.6440408579
52.66909219135-0.08805013498130.1077386215672.465829184350.393469360062.037042475830.08848759179270.1157250312950.111386770363-0.0638753934211-0.01519162355330.0736892784799-0.53448000435-0.10926124494-0.08521991242860.671880668957-0.01101465491760.04502690419810.2448128578960.03520995581730.317192316083-59.2065835084114.710676838-27.9219525762
62.903377697681.853151549451.200533993913.821596605660.220307443710.8435720205460.150677625268-0.2410593516940.5769422368920.255450220551-0.08583087654550.657559071511-0.559885437694-0.313709653138-0.05784608851630.6962610440980.1235396080920.09858386938170.307313037815-0.02368913205470.321917177282-74.7038272012110.435613102-23.212851649
71.913370563981.202474847940.415443960296.318274012391.767011176312.150398570750.09130343074080.0358729008380.071907870720.1429862564780.088635548484-0.29018092918-0.567209507110.319911562254-0.1555626993960.66719074396-0.05827048232810.08059817334110.344768532045-0.04038834417680.198377063515-54.7702718327107.294374148-15.9586355896
88.69837911117-1.266709278854.946213580345.40460090806-2.749925282544.222599523780.3818434923230.0740496304235-0.723387546135-0.147114037837-0.09563236164870.1941065853980.190591311899-0.364074883644-0.2367240635470.4772772379720.001338707681010.06013818124050.252033558312-0.03224851694090.353796719413-69.229029101990.7000749819-21.188846129
93.804478756721.220602064290.5443438707183.720235027340.8141454723192.944952194330.009626883341440.14819401562-0.336897100384-0.4529685281240.0269863810051-0.5664505233040.03811137867970.388819549553-0.01996411033150.3076375285230.08639496485560.07133795868970.529984015389-0.07171593173330.433996433621-21.759662530159.4689828586-33.8225530123
101.47196691116-0.3264730544520.2254143839381.98824677453-0.1478706698981.81911873131-0.0253318932443-0.00636679813840.0318675713676-0.1693149571510.0302068421799-0.339496249904-0.10781485680.3882722101980.004785942355460.217481662716-0.0681050640045-0.009224021583120.490809539345-0.03913808988010.354981384436-27.849607482572.1158855772-22.2246224138
113.725068919480.03088017546341.298458872353.93848039145-0.9640106982445.70691560894-0.01133165058730.538275799334-0.279363067778-0.775449004765-0.00020637996244-0.6491111100580.626244299730.698131603511-0.02071501164750.3965426882570.03573273496570.06910548886060.434754567023-0.1519666714540.517158680629-53.430525111420.4253251002-37.4142456019
122.568019436260.05366823364081.055659310476.37777302315-3.723718737575.921589184290.06797253282690.12096575824-0.0931427087155-0.3583568431380.2290224888560.7581019042050.640718889267-0.508991364333-0.1936043583190.40901162794-0.02037222480440.01687815738140.422777050616-0.007319903661780.446945873831-68.842835295122.5656284557-30.9462413103
134.91058957601-0.752962890283-0.2524153649332.43176258585-0.3968443472055.42406357083-0.01822064222250.292907165604-0.8308788271710.0372507335106-0.00286201559011-0.02915131896120.954289984018-0.404341112772-0.01624867616370.446744835310.0371814397661-0.00172169423540.293764282373-0.01067349164730.512292346043-59.521065168618.4052922458-24.9641743246
146.64345682446-0.0308469168083-0.6261351210143.516285961863.60048299933.979904038040.0387588402787-0.05932535712110.353525059490.29206164579-0.2182487406440.2597167531660.079516920484-0.466640248830.1413002710670.2960184905530.06884606948510.08669792009540.2707599883480.03051394624940.458562558389-65.337926570539.5024703769-20.0606911302
153.026176518670.3590020139890.1990340094113.62328724778-1.270453185332.4476291821-0.09784551890830.165997078832-0.3841738802260.1310157866540.155341895397-0.5969707025650.2874253937370.22957954195-0.06389505655550.3872568940950.128200679851-0.01233460082650.363701566602-0.116936120750.524361563662-46.227376710225.9200234073-28.5615338863
160.550891773303-0.280841699244-0.2529392546723.34262883638-0.997430997231.500734945550.04715610063-0.140178339839-0.4178385390240.296289130606-0.033225747276-0.8281651885830.1447263489010.507361525107-0.01599904585070.340478150130.144758405287-0.07129321969950.490691979621-0.005426629724850.641565538919-35.216240053735.8340907956-21.2079641871
173.9469094096-0.9054912553890.6516377682638.93526131022-2.243445541783.503450497820.16085971429-0.12245712247-0.4687484024620.345842070992-0.0754488636849-0.1777660189630.4764245023730.189119397786-0.08017253668090.3715130807720.0509546997785-0.03683762993240.355379809752-0.03031046476650.325023881333-54.530516413627.0587295321-15.8376905208
184.782846244352.32391981884-2.480074442684.70748233031-3.178100886318.77321708034-0.03130004469430.1119353392290.6860764202540.164496389068-0.1174536798420.124019201837-0.645866697072-0.01667919981160.1218362308740.353722838590.0680915267197-0.04200123700690.317580703489-0.04357218102910.527936042716-52.536028198548.9864469267-21.0564253581
191.246464394070.4464763524270.0187470976163.27113688143-0.1630220762532.193075065710.106738932750.293477348081-0.00896996217919-0.229381430187-0.1124657729840.478328842464-0.00215718000746-0.698597499757-0.01174889945510.2874075906780.01409053568580.02888412638090.63977299884-0.002772013440470.473355188413-108.05053325747.3062212487-28.4252659945
202.63043315943-0.299144055365-0.4947605154251.415246030370.1912838935411.838094198780.0827154810590.0385684683605-0.218462166909-0.0982847719492-0.08105868405460.0395628689920.219450158375-0.103888316990.0006071057295580.31965509499-0.08256753025390.03785784253870.369533346225-0.02631518687230.38763467024-92.598574654436.909523128-21.5997852479
212.518796655020.437299337579-0.371831511892.60901571495-0.1349334384291.635188171270.07624905699060.0251950948160.3605883914940.4057306514410.1461876370320.644228608511-0.719554057476-0.38033247718-0.1260666257530.5096202296570.230059266240.2579409104420.3932545934910.0322419430780.505453329118-99.4616615397.5188917483-25.5075632263
221.593012657760.6797423300080.1039409734872.93537986279-0.4808041189581.423340077440.0253468723790.0787956294223-0.03553755277790.2824342861570.1235236360650.620041935258-0.102896724561-0.473196291709-0.01701689069360.3238751351740.1660884097960.1824394320170.5415979595310.03214031445410.470584080593-104.97001471780.4794934897-23.7799432104
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 2 through 52 )AA2 - 521 - 51
22chain 'A' and (resid 53 through 88 )AA53 - 8852 - 85
33chain 'A' and (resid 89 through 141 )AA89 - 14186 - 138
44chain 'A' and (resid 142 through 199 )AA142 - 199139 - 181
55chain 'A' and (resid 200 through 285 )AA200 - 285182 - 245
66chain 'A' and (resid 286 through 379 )AA286 - 379246 - 339
77chain 'A' and (resid 380 through 434 )AA380 - 434340 - 394
88chain 'A' and (resid 435 through 467 )AA435 - 467395 - 427
99chain 'B' and (resid 2 through 70 )BB2 - 701 - 65
1010chain 'B' and (resid 71 through 467 )BB71 - 46766 - 426
1111chain 'C' and (resid 2 through 36 )CC2 - 361 - 35
1212chain 'C' and (resid 37 through 69 )CC37 - 6936 - 64
1313chain 'C' and (resid 70 through 141 )CC70 - 14165 - 137
1414chain 'C' and (resid 142 through 199 )CC142 - 199138 - 180
1515chain 'C' and (resid 200 through 296 )CC200 - 296181 - 254
1616chain 'C' and (resid 297 through 379 )CC297 - 379255 - 337
1717chain 'C' and (resid 380 through 434 )CC380 - 434338 - 392
1818chain 'C' and (resid 435 through 467 )CC435 - 467393 - 425
1919chain 'D' and (resid 2 through 164 )DD2 - 1641 - 161
2020chain 'D' and (resid 165 through 467 )DD165 - 467162 - 428
2121chain 'E' and (resid 2 through 224 )EE2 - 2241 - 204
2222chain 'E' and (resid 225 through 467 )EE225 - 467205 - 425

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