National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI150027-01
United States
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)
2T32DK007673
United States
National Institutes of Health/Office of the Director
S10OD020011
United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
U24 GM129539
United States
Other private
SF349247
United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
5R01AI095755-10
United States
Other government
1I01BX004447
United States
Citation
Journal: Elife / Year: 2020 Title: Structural analysis of the Dot/Icm type IV secretion system core complex. Authors: Clarissa L Durie / Michael J Sheedlo / Jeong Min Chung / Brenda G Byrne / Min Su / Thomas Knight / Michele Swanson / D Borden Lacy / Melanie D Ohi / Abstract: is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires' Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver ... is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires' Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver effector proteins into host cells. Compared to prototypical T4SSs, the Dot/Icm assembly is much larger, containing ~27 different components including a core complex reported to be composed of five proteins: DotC, DotD, DotF, DotG, and DotH. Using single particle cryo-electron microscopy (cryo-EM), we report reconstructions of the core complex of the Dot/Icm T4SS that includes a symmetry mismatch between distinct structural features of the outer membrane cap (OMC) and periplasmic ring (PR). We present models of known core complex proteins, DotC, DotD, and DotH, and two structurally similar proteins within the core complex, DotK and Lpg0657. This analysis reveals the stoichiometry and contact interfaces between the key proteins of the Dot/Icm T4SS core complex and provides a framework for understanding a complex molecular machine.
AV: Type IV secretion system unknown protein fragment AW: Type IV secretion system unknown protein fragment BV: Type IV secretion system unknown protein fragment BW: Type IV secretion system unknown protein fragment CV: Type IV secretion system unknown protein fragment CW: Type IV secretion system unknown protein fragment DV: Type IV secretion system unknown protein fragment DW: Type IV secretion system unknown protein fragment EV: Type IV secretion system unknown protein fragment EW: Type IV secretion system unknown protein fragment FV: Type IV secretion system unknown protein fragment FW: Type IV secretion system unknown protein fragment GV: Type IV secretion system unknown protein fragment GW: Type IV secretion system unknown protein fragment HV: Type IV secretion system unknown protein fragment HW: Type IV secretion system unknown protein fragment IV: Type IV secretion system unknown protein fragment IW: Type IV secretion system unknown protein fragment JV: Type IV secretion system unknown protein fragment JW: Type IV secretion system unknown protein fragment KV: Type IV secretion system unknown protein fragment KW: Type IV secretion system unknown protein fragment LV: Type IV secretion system unknown protein fragment LW: Type IV secretion system unknown protein fragment MV: Type IV secretion system unknown protein fragment MW: Type IV secretion system unknown protein fragment NV: Type IV secretion system unknown protein fragment NW: Type IV secretion system unknown protein fragment OV: Type IV secretion system unknown protein fragment OW: Type IV secretion system unknown protein fragment PV: Type IV secretion system unknown protein fragment PW: Type IV secretion system unknown protein fragment QV: Type IV secretion system unknown protein fragment QW: Type IV secretion system unknown protein fragment RV: Type IV secretion system unknown protein fragment RW: Type IV secretion system unknown protein fragment
Mass: 28102.492 Da / Num. of mol.: 36 / Source method: isolated from a natural source / Source: (natural) Legionella pneumophila (bacteria)
Sequence details
The authors state that the sequences are unknown. The sample was imaged from a purification that ...The authors state that the sequences are unknown. The sample was imaged from a purification that was derived directly from L. pneumophila and the polymer chains could not be identified.
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Experimental details
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Experiment
Experiment
Method: ELECTRON MICROSCOPY
EM experiment
Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction
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