+Open data
-Basic information
Entry | Database: PDB / ID: 6x66 | ||||||||||||||||||||||||
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Title | Legionella pneumophila dDot T4SS OMC | ||||||||||||||||||||||||
Components |
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Keywords | PROTEIN TRANSPORT / Secretion / T4SS / Dot | ||||||||||||||||||||||||
Function / homology | Function and homology information | ||||||||||||||||||||||||
Biological species | Legionella pneumophila (bacteria) | ||||||||||||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.2 Å | ||||||||||||||||||||||||
Authors | Durie, C.L. / Sheedlo, M.J. / Chung, J.M. / Byrne, B.G. / Su, M. / Knight, T. / Swanson, M.S. / Lacy, D.B. / Ohi, M.D. | ||||||||||||||||||||||||
Funding support | United States, 7items
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Citation | Journal: Elife / Year: 2020 Title: Structural analysis of the Dot/Icm type IV secretion system core complex. Authors: Clarissa L Durie / Michael J Sheedlo / Jeong Min Chung / Brenda G Byrne / Min Su / Thomas Knight / Michele Swanson / D Borden Lacy / Melanie D Ohi / Abstract: is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires' Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver ... is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires' Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver effector proteins into host cells. Compared to prototypical T4SSs, the Dot/Icm assembly is much larger, containing ~27 different components including a core complex reported to be composed of five proteins: DotC, DotD, DotF, DotG, and DotH. Using single particle cryo-electron microscopy (cryo-EM), we report reconstructions of the core complex of the Dot/Icm T4SS that includes a symmetry mismatch between distinct structural features of the outer membrane cap (OMC) and periplasmic ring (PR). We present models of known core complex proteins, DotC, DotD, and DotH, and two structurally similar proteins within the core complex, DotK and Lpg0657. This analysis reveals the stoichiometry and contact interfaces between the key proteins of the Dot/Icm T4SS core complex and provides a framework for understanding a complex molecular machine. | ||||||||||||||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 6x66.cif.gz | 2.5 MB | Display | PDBx/mmCIF format |
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PDB format | pdb6x66.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 6x66.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/x6/6x66 ftp://data.pdbj.org/pub/pdb/validation_reports/x6/6x66 | HTTPS FTP |
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-Related structure data
Related structure data | 22071MC 6x62C 6x64C 6x65C M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
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Noncrystallographic symmetry (NCS) | NCS domain:
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