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TitleStructural analysis of the Dot/Icm type IV secretion system core complex.
Journal, issue, pagesElife, Vol. 9, Year 2020
Publish dateSep 2, 2020
AuthorsClarissa L Durie / Michael J Sheedlo / Jeong Min Chung / Brenda G Byrne / Min Su / Thomas Knight / Michele Swanson / D Borden Lacy / Melanie D Ohi /
PubMed Abstract is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires' Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver ... is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires' Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver effector proteins into host cells. Compared to prototypical T4SSs, the Dot/Icm assembly is much larger, containing ~27 different components including a core complex reported to be composed of five proteins: DotC, DotD, DotF, DotG, and DotH. Using single particle cryo-electron microscopy (cryo-EM), we report reconstructions of the core complex of the Dot/Icm T4SS that includes a symmetry mismatch between distinct structural features of the outer membrane cap (OMC) and periplasmic ring (PR). We present models of known core complex proteins, DotC, DotD, and DotH, and two structurally similar proteins within the core complex, DotK and Lpg0657. This analysis reveals the stoichiometry and contact interfaces between the key proteins of the Dot/Icm T4SS core complex and provides a framework for understanding a complex molecular machine.
External linksElife / PubMed:32876045 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 4.2 Å
Structure data

22068

6x62

EMDB-22068, PDB-6x62:
Legionella pneumophila Dot T4SS OMC
Method: EM (single particle) / Resolution: 3.5 Å

22069

6x64

EMDB-22069, PDB-6x64:
Legionella pneumophila Dot T4SS PR
Method: EM (single particle) / Resolution: 3.7 Å

22070

6x65

EMDB-22070, PDB-6x65:
Legionella pneumophila Dot/Icm T4SS
Method: EM (single particle) / Resolution: 3.7 Å

22071

6x66

EMDB-22071, PDB-6x66:
Legionella pneumophila dDot T4SS OMC
Method: EM (single particle) / Resolution: 4.2 Å

Source
  • legionella pneumophila (bacteria)
KeywordsPROTEIN TRANSPORT / Secretion / T4SS / Dot

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