PDB-6wmi: ZNF410 zinc fingers 1-5 with 17 mer blunt DNA Oligonucleotide

ID or keywords:

Entry

Database: PDB / ID: 6wmi

Title

ZNF410 zinc fingers 1-5 with 17 mer blunt DNA Oligonucleotide

Components

DNA (5'-D(*CP*AP*CP*AP*TP*CP*CP*CP*AP*TP*AP*AP*TP*AP*AP*TP*G)-3')
DNA (5'-D(*CP*AP*TP*TP*AP*TP*TP*AP*TP*GP*GP*GP*AP*TP*GP*TP*G)-3')
Zinc finger protein 410

Keywords

TRANSCRIPTION/DNA / transcription factor / Zinc finger / Complex / DNA / TRANSCRIPTION / TRANSCRIPTION-DNA complex

Function / homology

Function and homology information

transcription coregulator activity / sequence-specific double-stranded DNA binding / regulation of transcription by RNA polymerase II / nucleus / metal ion binding
Similarity search - Function

Biological species

Homo sapiens (human)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

SAD / Resolution: 2.75 Å

Authors

Ren, R. / Horton, J.R. / Cheng, X.

Citation

Journal: Mol.Cell / Year: 2021
Title: ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression.
Authors: Lan, X. / Ren, R. / Feng, R. / Ly, L.C. / Lan, Y. / Zhang, Z. / Aboreden, N. / Qin, K. / Horton, J.R. / Grevet, J.D. / Mayuranathan, T. / Abdulmalik, O. / Keller, C.A. / Giardine, B. / ...

History

Deposition	Apr 21, 2020	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Dec 9, 2020	Provider: repository / Type: Initial release
Revision 1.1	Jun 23, 2021	Group: Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2	Mar 6, 2024	Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2 Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	6wmi.cif.gz	252.4 KB	Display	PDBx/mmCIF format
PDB format	pdb6wmi.ent.gz	165.9 KB	Display	PDB format
PDBx/mmJSON format	6wmi.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	6wmi_validation.pdf.gz	457.4 KB	Display	wwPDB validaton report
Full document	6wmi_full_validation.pdf.gz	458.2 KB	Display
Data in XML	6wmi_validation.xml.gz	15.7 KB	Display
Data in CIF	6wmi_validation.cif.gz	22.1 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/wm/6wmi ftp://data.pdbj.org/pub/pdb/validation_reports/wm/6wmi	HTTPS FTP

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Related structure data

Similar structure data	4kcd-2 2rca-1 1p14-d 3dzj-2 4yjq-d 5w86-2 2a5s-d 1i44-d 3pe2-d 3owj-d Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#255 - Mar 2021 Cisplatin and DNA similarity (2) #67 - Jul 2005 TATA-Binding Protein similarity (1) #168 - Dec 2013 DNA Helicase similarity (1) #91 - Jul 2007 Thymine Dimers similarity (1) #122 - Feb 2010 Enhanceosome similarity (5) #172 - Apr 2014 RecA and Rad51 similarity (1) #253 - Jan 2021 Expressome similarity (1) #181 - Jan 2015 Cascade and CRISPR similarity (1) #87 - Mar 2007 Zinc Fingers similarity (8) #112 - Apr 2009 Oct and Sox Transcription Factors similarity (7) #150 - Jun 2012 Sliding Clamps similarity (1) #135 - Mar 2011 Integrase similarity (1) #48 - Dec 2003 Catabolite Activator Protein similarity (1) #84 - Dec 2006 Transposase similarity (1) #227 - Nov 2018 Telomerase similarity (1) #180 - Dec 2014 TAL Effectors similarity (1) #139 - Jul 2011 DNA Methyltransferase similarity (1) #73 - Jan 2006 Topoisomerase similarity (1) #155 - Nov 2012 Vitamin D Receptor similarity (1) #23 - Nov 2001 DNA similarity (2) #40 - Apr 2003 RNA Polymerase similarity (1) #3 - Mar 2000 DNA Polymerase similarity (1) #257 - May 2021 Fetal Hemoglobin similarity (7) #55 - Jul 2004 DNA Ligase similarity (1) #8 - Aug 2000 Restriction Enzymes similarity (1) #7 - Jul 2000 Nucleosome similarity (1) #45 - Sep 2003 Estrogen Receptor similarity (1) #240 - Dec 2019 Hypoxia-Inducible Factors similarity (1) #39 - Mar 2003 lac Repressor similarity (1) #33 - Sep 2002 HIV Reverse Transcriptase similarity (1) #31 - Jul 2002 p53 Tumor Suppressor similarity (1) #258 - Jun 2021 Glucocorticoid Receptor and Dexamethasone similarity (1) #190 - Oct 2015 Two-component Systems similarity (1) #160 - Apr 2013 Actinomycin similarity (1) #131 - Nov 2010 Inteins similarity (1) #125 - May 2010 Parvoviruses similarity (1) #119 - Nov 2009 Designed DNA Crystal similarity (1) #92 - Aug 2007 Anabolic Steroids similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (1) #245 - May 2020 Spliceosomes similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

Related items in Molecule of the Month

#255 - Mar 2021
Cisplatin and DNA
similarity (2)
#67 - Jul 2005
TATA-Binding Protein
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#168 - Dec 2013
DNA Helicase
similarity (1)
#91 - Jul 2007
Thymine Dimers
similarity (1)
#122 - Feb 2010
Enhanceosome
similarity (5)
#172 - Apr 2014
RecA and Rad51
similarity (1)
#253 - Jan 2021
Expressome
similarity (1)
#181 - Jan 2015
Cascade and CRISPR
similarity (1)
#87 - Mar 2007
Zinc Fingers
similarity (8)
#112 - Apr 2009
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similarity (7)
#150 - Jun 2012
Sliding Clamps
similarity (1)
#135 - Mar 2011
Integrase
similarity (1)
#48 - Dec 2003
Catabolite Activator Protein
similarity (1)
#84 - Dec 2006
Transposase
similarity (1)
#227 - Nov 2018
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similarity (1)
#180 - Dec 2014
TAL Effectors
similarity (1)
#139 - Jul 2011
DNA Methyltransferase
similarity (1)
#73 - Jan 2006
Topoisomerase
similarity (1)
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Vitamin D Receptor
similarity (1)
#23 - Nov 2001
DNA
similarity (2)
#40 - Apr 2003
RNA Polymerase
similarity (1)
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DNA Polymerase
similarity (1)
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similarity (7)
#55 - Jul 2004
DNA Ligase
similarity (1)
#8 - Aug 2000
Restriction Enzymes
similarity (1)
#7 - Jul 2000
Nucleosome
similarity (1)
#45 - Sep 2003
Estrogen Receptor
similarity (1)
#240 - Dec 2019
Hypoxia-Inducible Factors
similarity (1)
#39 - Mar 2003
lac Repressor
similarity (1)
#33 - Sep 2002
HIV Reverse Transcriptase
similarity (1)
#31 - Jul 2002
p53 Tumor Suppressor
similarity (1)
#258 - Jun 2021
Glucocorticoid Receptor and Dexamethasone
similarity (1)
#190 - Oct 2015
Two-component Systems
similarity (1)
#160 - Apr 2013
Actinomycin
similarity (1)
#131 - Nov 2010
Inteins
similarity (1)
#125 - May 2010
Parvoviruses
similarity (1)
#119 - Nov 2009
Designed DNA Crystal
similarity (1)
#92 - Aug 2007
Anabolic Steroids
similarity (1)
#241 - Jan 2020
Twenty Years of Molecules
similarity (1)
#245 - May 2020
Spliceosomes
similarity (1)

Deposited unit

A: Zinc finger protein 410

D: Zinc finger protein 410

B: DNA (5'-D(*CP*AP*CP*AP*TP*CP*CP*CP*AP*TP*AP*AP*TP*AP*AP*TP*G)-3')

C: DNA (5'-D(*CP*AP*TP*TP*AP*TP*TP*AP*TP*GP*GP*GP*AP*TP*GP*TP*G)-3')

E: DNA (5'-D(*CP*AP*CP*AP*TP*CP*CP*CP*AP*TP*AP*AP*TP*AP*AP*TP*G)-3')

F: DNA (5'-D(*CP*AP*TP*TP*AP*TP*TP*AP*TP*GP*GP*GP*AP*TP*GP*TP*G)-3')

hetero molecules

56.9 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Zinc finger protein 410

E: DNA (5'-D(*CP*AP*CP*AP*TP*CP*CP*CP*AP*TP*AP*AP*TP*AP*AP*TP*G)-3')

F: DNA (5'-D(*CP*AP*TP*TP*AP*TP*TP*AP*TP*GP*GP*GP*AP*TP*GP*TP*G)-3')

hetero molecules

defined by author&software
Evidence: native gel electrophoresis
28.5 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

D: Zinc finger protein 410

B: DNA (5'-D(*CP*AP*CP*AP*TP*CP*CP*CP*AP*TP*AP*AP*TP*AP*AP*TP*G)-3')

C: DNA (5'-D(*CP*AP*TP*TP*AP*TP*TP*AP*TP*GP*GP*GP*AP*TP*GP*TP*G)-3')

hetero molecules

defined by author&software
28.5 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	186.257, 186.257, 46.950
Angle α, β, γ (deg.)	90.000, 90.000, 120.000
Int Tables number	171
Space group name H-M	P6₂
Space group name Hall	P6₂
Symmetry operation	#1: x,y,z #2: x-y,x,z+1/3 #3: y,-x+y,z+2/3 #4: -y,x-y,z+2/3 #5: -x+y,-x,z+1/3 #6: -x,-y,z

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Protein , 1 types, 2 molecules AD

#1: Protein	Zinc finger protein 410 / Another partner for ARF 1 / Zinc finger protein APA-1 Mass: 17656.416 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ZNF410, APA1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q86VK4

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DNA chain , 2 types, 4 molecules BECF

#2: DNA chain	DNA (5'-D(CPAPCPAPTPCPCPCPAPTPAPAPTPAPAPTPG)-3') Mass: 5139.376 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens* (human)
#3: DNA chain	DNA (5'-D(CPAPTPTPAPTPTPAPTPGPGPGPAPTPGPTPG)-3') Mass: 5272.430 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens* (human)

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Non-polymers , 3 types, 149 molecules

#4: Chemical	ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL Mass: 62.068 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₂H₆O₂
#5: Chemical	ChemComp-ZN / ZINC ION Mass: 65.409 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: Zn
#6: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 137 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 4.18 Å³/Da / Density % sol: 51 %
Crystal grow	Temperature: 292 K / Method: vapor diffusion, sitting drop / Details: 0.2 M ammonium formate, 20% PEG3350

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
Detector	Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 10, 2020
Radiation	Monochromator: M / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 2.75→41.92 Å / Num. obs: 47241 / % possible obs: 99.93 % / Redundancy: 11.1 % / B_iso Wilson estimate: 51.45 Å² / CC_1/2: 0.991 / CC star: 0.998 / R_merge(I) obs: 0.2 / R_pim(I) all: 0.07 / Net I/σ(I): 12.3
Reflection shell	Resolution: 2.75→2.85 Å / Redundancy: 10 % / R_merge(I) obs: 0.5 / Mean I/σ(I) obs: 1.3 / Num. unique obs: 4697 / CC_1/2: 0.498 / CC star: 0.815 / R_pim(I) all: 0.82 / % possible all: 100

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Processing

Software

Name	Version	Classification
PHENIX	1.17.1_3660	refinement
HKL-2000		data reduction
HKL-2000		data scaling
PHENIX		phasing

Refinement

Method to determine structure:

SAD / Resolution: 2.75→41.92 Å / SU ML: 0.3211 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 21.5442

	R_factor	Num. reflection	% reflection
R_free	0.1941	2365	5.01 %
R_work	0.1591	-	-
obs	0.1609	47202	99.93 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å

Displacement parameters

B_iso mean: 63.47 Å²

Refinement step

Cycle: LAST / Resolution: 2.75→41.92 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	2266	1382	18	137	3803

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0047	3882
X-RAY DIFFRACTION	f_angle_d	0.683	5508
X-RAY DIFFRACTION	f_chiral_restr	0.0384	583
X-RAY DIFFRACTION	f_plane_restr	0.004	475
X-RAY DIFFRACTION	f_dihedral_angle_d	23.4747	1506

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.75-2.81	0.3324	130	0.3055	2599	X-RAY DIFFRACTION	99.85
2.81-2.87	0.3115	138	0.2829	2643	X-RAY DIFFRACTION	99.89
2.87-2.94	0.3272	134	0.2831	2725	X-RAY DIFFRACTION	100
2.94-3.01	0.3041	137	0.2702	2542	X-RAY DIFFRACTION	99.96
3.01-3.09	0.3809	138	0.273	2665	X-RAY DIFFRACTION	100
3.09-3.18	0.2348	138	0.2028	2626	X-RAY DIFFRACTION	99.96
3.18-3.28	0.1748	142	0.1593	2644	X-RAY DIFFRACTION	100
3.28-3.4	0.1834	136	0.1379	2661	X-RAY DIFFRACTION	100
3.4-3.54	0.2404	146	0.1503	2641	X-RAY DIFFRACTION	100
3.54-3.7	0.2714	144	0.1677	2629	X-RAY DIFFRACTION	100
3.7-3.89	0.1624	140	0.146	2668	X-RAY DIFFRACTION	100
3.89-4.14	0.1905	142	0.1373	2598	X-RAY DIFFRACTION	100
4.14-4.46	0.168	142	0.1281	2635	X-RAY DIFFRACTION	99.96
4.46-4.9	0.1563	146	0.1352	2643	X-RAY DIFFRACTION	99.96
4.9-5.61	0.1556	138	0.1216	2638	X-RAY DIFFRACTION	99.93
5.61-7.06	0.1598	137	0.1366	2644	X-RAY DIFFRACTION	99.86
7.07-41.92	0.1132	137	0.1284	2636	X-RAY DIFFRACTION	99.46

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.05696284666	0.873549621372	-5.25900076212	6.39696096075	0.351746424241	7.43043701045	-0.233595119018	0.651985586995	-0.424790419428	-1.05996308216	-0.180386393352	-0.64444845856	0.455402147556	0.573842264124	0.339491650624	0.424127429947	0.102362976578	0.0557366704679	0.801444041236	-0.0254428784836	0.38631032636	-20.8589118932	66.4672728273	-19.0451996392
2	2.98227209208	-1.42573798873	-0.964990269688	7.29766801431	1.64498781014	5.73551130415	0.105368122793	0.400295156693	0.381950911084	-0.416816972893	-0.0091511683385	-0.907208693294	-0.667531736867	0.718845763292	-0.00814235547732	0.324035252066	-0.155534053196	0.0469190429464	0.712991953475	0.0336285868813	0.354889943112	-20.2033673189	86.1376974389	-8.68987755431
3	2.0567602011	4.23433806236	-5.60008251755	5.55437083646	-3.27388092797	2.01793313686	0.1823766884	-0.154888177182	0.443483224896	0.291806318832	0.313836971955	0.195106490686	-0.330444100283	0.0277866555524	-0.616635136709	0.314324130814	-0.0829404966446	-0.0266232311709	0.629540644935	-0.0170294248437	0.321944695063	-26.0358017525	83.3042396339	12.6136287444
4	4.64499769406	-4.03149875512	-0.938715574954	2.09932601479	0.649622909968	2.10724622785	-0.513055734887	-0.305895294586	-0.718330134809	0.205480581272	0.48648273393	0.10872631279	0.694449586124	-0.0607203502703	-0.0695313817333	0.486993358457	-0.00642533266272	0.0152452030409	0.607018587482	0.0488098710605	0.410325153968	-17.8336168826	66.4060677445	13.3891248386
5	9.99249195734	-0.623093079629	-4.95697312504	2.00937502203	2.95910043469	7.25553008308	-0.46545808762	0.64054897744	-1.70370538819	-0.736308578997	-0.188474635103	-1.31744241724	1.0314818695	1.11550497658	0.612755387495	0.561751934702	0.186542852474	0.0902178110739	1.0544009544	-0.0130759468667	0.728244877871	-7.6976123786	64.2059156157	7.30734315634
6	2.03171137842	0.203032484769	-1.79586444667	0.0633876864127	-0.26108627671	1.76157369023	-0.666979824339	0.954670858887	-0.565385169935	-0.377248442987	-0.109907144269	-0.904571641424	-0.00987853949968	0.451813630892	0.682846742782	0.431724083381	0.0753233417671	-0.0132450965601	1.71260431209	0.170923372044	1.37885089128	5.28233529637	72.1548605497	3.26850304012
7	0.425175738423	-2.01106615306	-1.10729397	9.51207461194	5.25130462056	2.89722412704	-0.766242977666	1.02900830027	-0.677922995709	-0.254787158295	1.08486751997	-3.11720379418	-1.4617317641	2.25592521937	-0.330521099677	0.756200095259	-0.386656274524	-0.2131028868	1.87103741864	0.30376864778	1.41381237298	5.46018119809	80.7205186732	6.97501462239
8	8.62392779672	5.46267252319	-5.35802618726	2.08189254489	-4.10679628953	3.63465476234	0.0792615488407	-0.437411723517	-0.501723899159	0.684855126853	-0.238559038488	-0.120233989583	0.228870914458	0.00656233524018	0.182982632114	0.575985808108	-0.0627791575463	-5.19534236507E-5	0.53809690075	-0.00475102920455	0.281447916213	-40.9304545522	57.0267867795	29.2131831746
9	7.20409851667	-5.00328128356	3.00308078555	6.68898037484	-3.42676890347	7.4973240163	0.0103387630544	-0.35269857177	-0.423918792747	0.220825416718	0.25033475795	0.583621270639	0.0587932163143	-0.749007698785	-0.237171822069	0.321524583203	-0.193001881125	0.0410580835757	0.445822886531	-0.0527489200514	0.301508365206	-56.9685627288	68.2617375957	18.5968940219
10	9.76081811524	1.42002073219	-2.26145965576	5.83677596276	0.386299739295	7.55746447321	0.307456151766	0.263504128857	0.711730838169	-0.760273383545	-0.331560257856	-0.0518812774645	-0.805957541548	0.0150488526194	0.0181936758483	0.354519080124	-0.0699929879662	-0.059248110409	0.499702824943	0.147607895694	0.381323564201	-49.640873755	72.168610084	-2.38280238645
11	7.34221589529	-4.75736377712	-2.57997130991	9.63614782357	1.8867016302	5.75027214309	-0.149769213652	0.243184843564	-0.349848737764	-0.125724700319	0.0134211656042	-0.128482269764	0.630029604921	0.368062648221	0.11399603137	0.365953950718	-0.150579530782	0.00977743369006	0.440946268424	-0.0638323984077	0.268330725936	-44.1644176288	57.2078474055	-2.76972608316
12	2.02770400689	4.71912592683	-7.47358737082	4.4642264599	-5.52244137542	7.24806484266	-0.549792613282	-0.838884728669	-1.57371091336	0.127998318175	-0.446127962158	0.742229945478	1.53983820606	-0.114213515992	0.814097339847	1.06525894337	-0.240872012081	0.1571214912	0.664160272908	0.00678442365806	1.06355246997	-54.3841150015	41.7264101459	5.45124489874
13	2.00088490059	-2.58635108922	8.49439432652	7.52236919529	1.24602707285	6.82048679208	0.627484408424	-1.77478583258	-3.07099870393	-0.165428631392	-0.729246832545	0.471454288266	1.3724330142	-2.88007482076	0.0878399750772	1.07278004167	-0.499640341968	0.2103704884	1.37765188278	-0.393871716996	1.3974604661	-67.726697325	44.3499967232	3.59338560233
14	6.31750687273	3.12786752041	2.53380860306	7.19195002125	1.45713850405	1.02314720284	0.07701374529	0.135555639369	-1.58840830203	0.838018808217	-0.0753189797244	0.290819352374	1.81681871244	-0.679780816972	-0.0299258841865	1.31583691197	-0.438088157093	-0.0412342699137	0.771149523142	-0.264456310547	0.912720561019	-55.3880415657	44.4728678505	-4.14168099097
15	4.09284760041	-1.36869397538	-0.399526829489	0.399263470525	-0.14537030935	7.37370074048	0.0160058826735	-0.48064373721	0.497656332609	-0.0790833216432	-0.0449083427123	-0.124150187378	0.488498073276	-0.125913494949	-0.00617864061593	0.304065465102	-0.0512703803884	-0.00600002419962	0.23604499414	-0.056343334908	0.367884860808	-45.2213991491	63.090025588	12.7551377703
16	8.92974701299	-0.148780649379	3.34227706615	7.32469123986	4.9951377003	8.77445904629	0.180697222335	-0.461360843729	-0.0985556671407	0.243715875718	0.462902813653	-0.509284202785	0.589990567057	-0.571686208096	-0.678883716814	0.377115882346	-0.141555687613	-0.0684190397857	0.472779152011	-0.0162543217366	0.336797483145	-41.897987085	72.8135770502	20.6080147791
17	3.42350941556	-1.90489125042	3.41210432338	2.01259472628	-0.876163127941	3.88208451566	-0.0555446317505	0.284508223719	-0.45483695551	-0.867809026525	0.347071690771	0.0680159915836	0.46271891706	0.252358608038	-0.220884247801	0.39744875343	-0.0599069562216	0.0447199820033	0.376556843454	-0.107926303677	0.347229337723	-44.5967329889	57.7997021909	10.0703514492
18	7.7607811051	-2.1606861104	1.94019841078	8.95095043336	8.59248719995	2.01220860726	0.288586605734	0.163748489455	-1.31879846763	-1.54719081636	-0.232643865442	0.953792077028	-0.23703316477	-2.36994938755	-0.0972600068157	0.752401170967	-0.278471689954	-0.113335409406	0.824186589851	-0.0778169913361	0.561500931629	-59.6979138606	53.2236543249	-0.400485790907
19	4.50931469169	-0.30628793081	-3.67902445113	1.49905820583	3.63207608936	2.0025937149	-0.152447273683	0.494974494517	0.243078655491	-1.12008315203	0.109661776078	-0.0214876537207	-0.793308698463	0.152272457639	0.0534118575124	1.48020661916	-0.41160333533	0.00410768255852	0.971172364147	-0.515969257426	0.968761987272	-58.4842435648	45.3281961532	-12.9709786818
20	8.02891544476	6.3299551314	2.08365290374	6.6721070811	1.17586663334	0.673544131775	0.665155130951	0.534156118071	-0.67501623661	0.0456558092469	-0.976435450387	-2.07233257876	0.316465838156	1.37758085423	0.289640093333	0.486061202186	0.0916011730622	-0.252865662496	1.38772263507	0.0444098392879	1.08377781659	-2.1975161667	71.0040035374	14.5432697092
21	1.27686817101	1.57431773829	0.75986454425	4.01805257974	1.15548048525	4.50746574686	-0.265886240719	0.177014767578	0.365937518375	-0.18577490222	0.186603157261	0.34318577114	0.00197028792247	0.375011025109	0.0736044735498	0.206954296795	-0.01550995006	-0.0289377937608	0.514655984018	0.0314953966431	0.355955493031	-23.1099278354	74.1020419815	-2.44421435452
22	9.34809157986	-2.64246685235	6.92042730751	5.78122317143	-1.45716034513	5.2744808327	0.0325974669736	0.000704187014634	-0.351118774826	-0.579276868831	0.630948990998	0.175331024341	0.159991426785	0.505810263129	-0.737567406268	0.355122737134	-0.0651301857481	-0.0522179692803	0.623643877331	-0.103929654105	0.339244024524	-32.884981014	77.2626173304	-10.2954007654
23	2.00641665854	-3.26573176798	6.03741637874	8.76043118268	1.33053690416	6.88303847194	-0.0471343428221	-0.475890448363	0.0246713138823	0.120561364922	0.621195688593	-0.391812606319	0.494178697973	0.302324798625	-0.581685211522	0.311630868015	-0.0165711777871	-0.048593646315	0.63505861891	0.00732468107908	0.31279434234	-19.2908794931	70.4311350283	0.363278286068
24	4.39071650232	-2.0975441332	7.77998108006	2.02551179368	-3.93502883533	2.01275741387	-0.785577813298	-0.465910267116	-0.105814049969	0.945092624036	0.476092684391	-1.82097416059	-1.92630123675	0.634941905696	0.286322965805	0.65168791982	-0.151640659525	-0.202456290791	1.25752473098	-0.175217064489	0.712577575134	-6.45226676372	79.8075990986	10.663261428
25	6.72796341234	2.8513294776	-0.332982891398	6.08488785127	-5.0777034734	8.79874710318	-0.785427527662	-1.29689612464	-0.00573338078835	1.58717967495	0.12332904033	-0.969187829511	-0.973730569234	-0.526311763112	0.563709693219	0.749684861338	0.214326256496	-0.476435967604	1.82606695672	-0.00830832931361	0.975455907284	-0.871317552357	74.2013525424	23.2346034558

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 217 through 244 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 245 through 274 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 275 through 292 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 293 through 322 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 323 through 334 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 335 through 350 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 351 through 362 )
8	X-RAY DIFFRACTION	8	chain 'D' and (resid 217 through 244 )
9	X-RAY DIFFRACTION	9	chain 'D' and (resid 245 through 274 )
10	X-RAY DIFFRACTION	10	chain 'D' and (resid 275 through 284 )
11	X-RAY DIFFRACTION	11	chain 'D' and (resid 285 through 322 )
12	X-RAY DIFFRACTION	12	chain 'D' and (resid 323 through 350 )
13	X-RAY DIFFRACTION	13	chain 'D' and (resid 351 through 362 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 1 through 5 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 6 through 17 )
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 1 through 5 )
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 6 through 10 )
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 11 through 15 )
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 16 through 17 )
20	X-RAY DIFFRACTION	20	chain 'E' and (resid 1 through 5 )
21	X-RAY DIFFRACTION	21	chain 'E' and (resid 6 through 17 )
22	X-RAY DIFFRACTION	22	chain 'F' and (resid 1 through 5 )
23	X-RAY DIFFRACTION	23	chain 'F' and (resid 6 through 10 )
24	X-RAY DIFFRACTION	24	chain 'F' and (resid 11 through 15 )
25	X-RAY DIFFRACTION	25	chain 'F' and (resid 16 through 17 )

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