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- PDB-6vm9: T4H2 T cell receptor bound to HLA-A2 presenting gp100T2M peptide ... -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 6vm9
TitleT4H2 T cell receptor bound to HLA-A2 presenting gp100T2M peptide (IMDQVPFSV)
Components
  • Beta-2-microglobulin
  • MHC class I antigen, A-2 alpha chain
  • Melanocyte protein PMEL
  • T4H2 T cell receptor alpha chain
  • T4H2 T cell receptor beta chain
KeywordsIMMUNE SYSTEM / TCR / MHC class I / HLA A2 / melanoma
Function / homology
Function and homology information


cis-Golgi network membrane / positive regulation of melanin biosynthetic process / melanin biosynthetic process / melanosome membrane / melanosome organization / multivesicular body, internal vesicle / multivesicular body membrane / antigen processing and presentation of peptide antigen via MHC class I / Regulation of MITF-M-dependent genes involved in pigmentation / : ...cis-Golgi network membrane / positive regulation of melanin biosynthetic process / melanin biosynthetic process / melanosome membrane / melanosome organization / multivesicular body, internal vesicle / multivesicular body membrane / antigen processing and presentation of peptide antigen via MHC class I / Regulation of MITF-M-dependent genes involved in pigmentation / : / : / positive regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / negative regulation of receptor binding / DAP12 interactions / cellular response to iron ion / lumenal side of endoplasmic reticulum membrane / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / peptide antigen assembly with MHC class II protein complex / cellular response to iron(III) ion / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / MHC class II protein complex / negative regulation of forebrain neuron differentiation / ER to Golgi transport vesicle membrane / peptide antigen assembly with MHC class I protein complex / regulation of erythrocyte differentiation / regulation of iron ion transport / MHC class I peptide loading complex / response to molecule of bacterial origin / HFE-transferrin receptor complex / T cell mediated cytotoxicity / antigen processing and presentation of endogenous peptide antigen via MHC class I / positive regulation of T cell cytokine production / antigen processing and presentation of exogenous peptide antigen via MHC class II / MHC class I protein complex / positive regulation of immune response / peptide antigen binding / negative regulation of neurogenesis / positive regulation of T cell mediated cytotoxicity / positive regulation of receptor-mediated endocytosis / multicellular organismal-level iron ion homeostasis / positive regulation of T cell activation / cellular response to nicotine / specific granule lumen / recycling endosome membrane / phagocytic vesicle membrane / positive regulation of cellular senescence / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / negative regulation of epithelial cell proliferation / Interferon gamma signaling / MHC class II protein complex binding / positive regulation of protein binding / Modulation by Mtb of host immune system / late endosome membrane / sensory perception of smell / tertiary granule lumen / melanosome / DAP12 signaling / negative regulation of neuron projection development / iron ion transport / T cell differentiation in thymus / ER-Phagosome pathway / protein refolding / early endosome membrane / protein homotetramerization / amyloid fibril formation / intracellular iron ion homeostasis / learning or memory / immune response / Amyloid fiber formation / endoplasmic reticulum lumen / Golgi membrane / external side of plasma membrane / lysosomal membrane / focal adhesion / Neutrophil degranulation / endoplasmic reticulum membrane / SARS-CoV-2 activates/modulates innate and adaptive immune responses / structural molecule activity / cell surface / endoplasmic reticulum / Golgi apparatus / protein homodimerization activity / extracellular space / extracellular exosome / extracellular region / identical protein binding / membrane / plasma membrane / cytosol
Similarity search - Function
PKD- and KLD-Associated Transmembrane / PKAT, KLD domain / PKAT, KLD domain / PKD domain / Polycystic kidney disease (PKD) domain profile. / PKD domain / PKD domain superfamily / PKD/Chitinase domain / Repeats in polycystic kidney disease 1 (PKD1) and other proteins / MHC class I, alpha chain, C-terminal ...PKD- and KLD-Associated Transmembrane / PKAT, KLD domain / PKAT, KLD domain / PKD domain / Polycystic kidney disease (PKD) domain profile. / PKD domain / PKD domain superfamily / PKD/Chitinase domain / Repeats in polycystic kidney disease 1 (PKD1) and other proteins / MHC class I, alpha chain, C-terminal / MHC_I C-terminus / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / : / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
Major histocompatibility complex, class I, A / MHC class I antigen / Melanocyte protein PMEL / Beta-2-microglobulin
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.9 Å
AuthorsSmith, A.R. / Baker, B.M.
Funding support1items
OrganizationGrant numberCountry
National Institutes of Health/National Cancer Institute (NIH/NCI)
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2021
Title: Structurally silent peptide anchor modifications allosterically modulate T cell recognition in a receptor-dependent manner
Authors: Smith, A.R. / Alonso, J.A. / Ayres, C.M. / Singh, N.K. / Hellman, L.M. / Baker, B.M.
History
DepositionJan 27, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 20, 2021Provider: repository / Type: Initial release
Revision 1.1Jan 27, 2021Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.pdbx_database_id_DOI / _citation.title / _citation.year
Revision 1.2Oct 11, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.3Oct 9, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: MHC class I antigen, A-2 alpha chain
B: Beta-2-microglobulin
C: Melanocyte protein PMEL
D: T4H2 T cell receptor alpha chain
E: T4H2 T cell receptor beta chain


Theoretical massNumber of molelcules
Total (without water)94,9655
Polymers94,9655
Non-polymers00
Water00
1
A: MHC class I antigen, A-2 alpha chain
B: Beta-2-microglobulin
C: Melanocyte protein PMEL

D: T4H2 T cell receptor alpha chain
E: T4H2 T cell receptor beta chain


Theoretical massNumber of molelcules
Total (without water)94,9655
Polymers94,9655
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation4_655-x+1,-y,z1
Unit cell
Length a, b, c (Å)129.744, 129.744, 113.440
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number172
Space group name H-MP64
Space group name HallP64
Symmetry operation#1: x,y,z
#2: x-y,x,z+2/3
#3: y,-x+y,z+1/3
#4: -y,x-y,z+1/3
#5: -x+y,-x,z+2/3
#6: -x,-y,z

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Components

#1: Protein MHC class I antigen, A-2 alpha chain


Mass: 31854.203 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-A / Plasmid: pHN1 / Production host: Escherichia coli (E. coli) / Strain (production host): XL1-Blue / References: UniProt: A0A5B8RNS7, UniProt: A0A140T913*PLUS
#2: Protein Beta-2-microglobulin


Mass: 11879.356 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P / Plasmid: pHN1 / Production host: Escherichia coli (E. coli) / Strain (production host): XL1-Blue / References: UniProt: P61769
#3: Protein/peptide Melanocyte protein PMEL / ME20-M / ME20M / Melanocyte protein Pmel 17 / Melanocytes lineage-specific antigen GP100 / Melanoma- ...ME20-M / ME20M / Melanocyte protein Pmel 17 / Melanocytes lineage-specific antigen GP100 / Melanoma-associated ME20 antigen / P1 / P100 / Premelanosome protein / Silver locus protein homolog


Mass: 1035.213 Da / Num. of mol.: 1 / Fragment: epitope (UNP residues 209-217) / Mutation: T210M / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P40967
#4: Protein T4H2 T cell receptor alpha chain


Mass: 22768.986 Da / Num. of mol.: 1 / Mutation: 0
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TRAV12-2, TRAC / Plasmid: pGMT7 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS
#5: Protein T4H2 T cell receptor beta chain


Mass: 27427.480 Da / Num. of mol.: 1 / Mutation: 0
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TRBV19, TRBC2 / Plasmid: pGMT7 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.9 Å3/Da / Density % sol: 57.62 %
Crystal growTemperature: 296 K / Method: vapor diffusion, hanging drop / pH: 8.5
Details: 18% v/v PEG10000, 16% v/v glycerol, 250 mM Tris pH 8.5, 60 mM sodium chloride, seeded with crushed crystals

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Aug 16, 2018
RadiationMonochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.9→50 Å / Num. obs: 24229 / % possible obs: 99.6 % / Redundancy: 9.6 % / Biso Wilson estimate: 88 Å2 / CC1/2: 0.996 / CC star: 0.999 / Rmerge(I) obs: 0.097 / Rpim(I) all: 0.033 / Rrim(I) all: 0.103 / Χ2: 1.111 / Net I/σ(I): 24.2
Reflection shellResolution: 2.9→2.95 Å / Redundancy: 7.1 % / Rmerge(I) obs: 0.882 / Mean I/σ(I) obs: 1.7 / Num. unique obs: 1125 / CC1/2: 0.864 / CC star: 0.963 / Rpim(I) all: 0.324 / Rrim(I) all: 0.943 / Χ2: 0.961 / % possible all: 94.9

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Processing

Software
NameVersionClassification
PHENIX1.13_2998refinement
PHENIX1.13_2998refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 1TVB

1tvb


Resolution: 2.9→42.7 Å / SU ML: 0.4764 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 31.716
RfactorNum. reflection% reflection
Rfree0.2501 1836 8.19 %
Rwork0.2104 --
obs0.2137 22405 92.11 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 103.23 Å2
Refinement stepCycle: LAST / Resolution: 2.9→42.7 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6642 0 0 0 6642
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00216825
X-RAY DIFFRACTIONf_angle_d0.48889271
X-RAY DIFFRACTIONf_chiral_restr0.0426964
X-RAY DIFFRACTIONf_plane_restr0.00281218
X-RAY DIFFRACTIONf_dihedral_angle_d19.53862509
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.89-2.970.51061020.4061186X-RAY DIFFRACTION69.28
2.97-3.060.40221200.37921300X-RAY DIFFRACTION76.8
3.06-3.160.32941280.29861423X-RAY DIFFRACTION83.3
3.16-3.270.31571420.28741541X-RAY DIFFRACTION89.47
3.27-3.40.31311350.28151577X-RAY DIFFRACTION92.19
3.4-3.550.3461420.26031605X-RAY DIFFRACTION94.48
3.55-3.740.30421540.24811674X-RAY DIFFRACTION97.03
3.74-3.980.25261540.22971661X-RAY DIFFRACTION98.21
3.98-4.280.24571540.20121698X-RAY DIFFRACTION98.88
4.28-4.710.24091520.17271700X-RAY DIFFRACTION99.36
4.71-5.390.18241520.1721734X-RAY DIFFRACTION99.42
5.39-6.790.24051510.19831723X-RAY DIFFRACTION99.68
6.79-42.70.20921500.17561747X-RAY DIFFRACTION98.91
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
15.150661035351.233433098771.124967968072.126898155020.9011720833042.914675199950.4616415617210.513139459849-0.738107704019-0.229781329719-0.0656160676961-0.2280783227340.3792424389690.133862967948-0.2706063780851.064185050390.365132895451-0.06331952760050.624117833368-0.1074889942580.80785615119399.9260558676-7.71802376726-33.6183556195
28.630067239350.9994628977761.62078354653.9176995786-1.443761376932.628942281570.219878071207-0.911126246544-0.5838747337140.5152026540020.008061924123640.09744357334930.244350119650.130759422319-0.2756785833441.002053270360.167673830288-0.0187664536260.811730439039-0.04009689227770.72887750065997.2881627144-8.15768544102-21.9960556934
32.34214145319-0.3770683200891.250939280740.571617573529-0.7731455546216.8240728671-0.08447763913530.2074223129-0.0407084176653-0.400013610616-0.453293846810.6715771014270.194689589031-0.5710627315360.3736071585571.200441703820.409617975521-0.1468316949250.885384132079-0.2220206694630.82964895331689.8953325494-2.5587868098-35.2892754662
43.691762667530.9266587698031.528848093711.12374440856-0.5993030115951.985771462340.3976440646390.894807539984-0.283562565246-0.122385547366-0.0180204504316-0.1788037106850.4940166431450.506130225535-0.2635206282281.176690156030.406857951818-0.05993192279550.777056248869-0.07863572579380.76994699393102.5773842860.245679126234-40.9501670174
55.71854777333-0.5014844721760.2192020698945.215501103821.926527831333.24632077750.374092460281.10970912550.294302057923-1.29208372447-0.2551226090460.208370100119-0.457174894721-0.0699852882001-0.1749204229821.249736733110.3983028532790.02238547532760.8339107197040.08121848604940.73364578843199.66131336088.37487989923-45.9757807316
65.403152576580.24007691806-1.430884236280.02348381921110.3113040740674.303526399430.34718982078-0.5085034062210.5043993110280.03086222075180.2666834846110.211010373215-0.358519985310.46644148235-0.5734932887150.9095873632440.147571140205-0.0921530759480.512812728628-0.0914954633960.706401408512106.3268129526.23977620763-22.2657055646
72.95126704955-0.2926042631540.173079941772.82103784886-0.9277184341910.200446977570.007399797361270.136993214407-0.508557802692-0.032825288985-0.256913324065-0.323720680176-0.155286151130.0915034834830.1988264058521.05516979350.333555380481-0.1017930105160.796822440603-0.02315565516251.03915419464126.830432069-16.4383279078-27.9988901081
86.507853142322.611422049970.1930568359011.67583912539-1.568096520652.8276869046-0.2205935085630.508097185932-0.764709653602-0.277529428050.08868041011-0.2719739347330.3286294820980.3412116311390.1680911225741.005245324050.340076872269-0.05439246549790.782434323049-0.1163089107210.9446441884131.40161851-15.736146923-35.0128446787
95.280483623790.851903326612-1.398310712382.998774459931.71424721373.90038542429-0.2413447684810.106552090858-0.202435904682-0.120423954337-0.488811464897-0.680443079616-0.409573623626-0.3876681774720.5664632969641.049055926960.182280515475-0.02660580453560.630397599217-0.1294766100980.943854639121113.944520745-22.7767319704-34.9475189191
104.009281172640.22302892812-0.1768256070663.37361757419-0.1261394182684.20952390192-0.00801930840832-0.233956279973-0.5169710031880.200411812682-0.2940912416450.380686694644-0.335183209462-0.6641827954790.2958902595790.987423571880.232185251108-0.01208785874940.698938413303-0.1773662186870.932246416734105.811128937-24.3976774906-33.7222646908
114.229817784710.6448276245051.977667932993.14130808976-0.7883558048136.26617892250.2214882110420.00847146498035-0.463900323202-0.54737946208-0.383719222769-0.1833602904940.384281168671-0.3239387419650.2040903338690.9668339709850.2417474934520.02256752421110.599457151637-0.1024825108750.840281125669110.371818964-24.8606227684-33.6744192983
125.05107966772-0.06655885901142.078048476062.762532310750.2654782354255.49017860474-0.1324075760210.258831885517-0.73220649228-0.1936195061470.433086987238-0.108280343599-0.06216369011580.431007370231-0.2918779966961.33466247520.3502634087640.05564464053160.707899534541-0.1015728293630.981281430397112.997116365-29.0843984283-40.8519734202
132.555656430510.50363740339-0.9012135203954.917800598321.830689069911.1895906075-1.830512534880.08951789106880.358389450905-0.53170964315-0.1612380973730.796989600618-1.5423468379-0.4245695898431.993179455871.198496908530.264559525616-0.2157971434780.966820383165-0.0961950296040.78481233461593.54429916414.428442803-36.044092086
143.70484488432.298333920760.5483370739412.072015175550.549157022934.940976590970.30025041033-0.650661200211-0.8503693937281.58291507958-0.0248942702823-0.8350749835840.275853437335-0.437880356747-0.1823621510221.05101312930.100384622622-0.07383039788080.7899884289440.2227598965451.0662483571943.083836603-30.570215401-19.9681216376
156.035814653783.110111720970.9929678557485.08221826039-1.493890776884.07915856330.03545524458410.0549845829575-0.4570922643090.05543799008590.01738140526140.1739462874240.0587262956788-0.207717345508-0.04148902888580.7705871597650.1961042738350.04431483954670.555830732290.04429473297460.62086849644740.9080615744-23.9181403457-28.1369305
160.912032701323-0.334305552040.4903551664980.0278336747025-0.1207738940122.30963035698-0.10321612361-0.227995533778-0.8123980849050.863513270312-0.392756622785-0.09690899463890.850939874104-0.2767907785380.3289373298611.236753663720.07883980053740.1021818213750.8099481032230.156505378531.4144073789151.7543418831-44.221123487-22.6849251234
173.825918881191.55261282427-0.08988966701355.013049783580.8474241508737.794302216850.0161617353651-0.179178688412-0.5812881381460.991212029660.186399344746-0.2459602558750.6423824729921.53965736122-0.3023733820531.319866423380.3322642103820.01832849464521.387117620670.1629911594361.1256496412269.4750852241-47.705178522-16.00658104
184.785087840271.46727705476-0.286596226246.412701982210.1839662772665.95742249528-0.707605874793-0.00708925721796-0.277212176594-0.1458105721770.2730133664780.2422317960951.220682892120.4522472525760.6088708810651.410257138650.155188489589-0.02536851990960.8195757084230.1788546936821.1905667646962.1034962904-45.6117130637-25.2078605629
193.330227845721.312619151130.909125589954.856631426290.07693082506474.770974794760.214658978948-1.81432832511-0.8198233787490.9632104401130.0452046551221-0.09813742167030.851402366285-0.871636741655-0.1327874482081.779831070440.243774110143-0.01667021929631.593634512620.3442181873891.2591730704967.0183036386-50.7762893435-13.2364378317
205.663989434070.8142581707584.376314905524.14517171712-0.8506499187326.20187350150.4677203441610.1390129269350.478547800771-0.569229143333-0.204651890763-0.492995214885-0.1925616795330.261213777748-0.2736542357720.8584876558140.2009612422830.2724594291810.7650747879390.005973911028910.89396942744759.2242508039-8.88969780237-25.9487735488
213.33888115731-2.36827052642-0.03769578214632.621706625430.6202007825513.02531437706-0.295777309092-0.769362161748-0.1008599507290.1379457414860.508007751106-0.0234233564442-0.3056972131920.330895698241-0.1083230470150.791955147250.2194546006050.1665934227630.8065224103450.0009797627991280.80605767390163.4748361347-17.0775724862-24.6574878773
222.263427095163.057208454070.7597060236544.539866808441.834762865973.28650842474-0.0704691884314-0.344439913686-0.3282565747560.3586819887030.295059361485-0.2688539709770.41768696660.341524575052-0.1652169088360.8693407893620.341978144442-0.07646526967880.998239828398-0.01618834739250.88606217663174.6700399117-38.2377163473-26.6643616419
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 37 )
2X-RAY DIFFRACTION2chain 'A' and (resid 38 through 56 )
3X-RAY DIFFRACTION3chain 'A' and (resid 57 through 84 )
4X-RAY DIFFRACTION4chain 'A' and (resid 85 through 118 )
5X-RAY DIFFRACTION5chain 'A' and (resid 119 through 162 )
6X-RAY DIFFRACTION6chain 'A' and (resid 163 through 174 )
7X-RAY DIFFRACTION7chain 'A' and (resid 175 through 197 )
8X-RAY DIFFRACTION8chain 'A' and (resid 198 through 275 )
9X-RAY DIFFRACTION9chain 'B' and (resid 1 through 20 )
10X-RAY DIFFRACTION10chain 'B' and (resid 21 through 57 )
11X-RAY DIFFRACTION11chain 'B' and (resid 58 through 84 )
12X-RAY DIFFRACTION12chain 'B' and (resid 85 through 100 )
13X-RAY DIFFRACTION13chain 'C' and (resid 1 through 9 )
14X-RAY DIFFRACTION14chain 'D' and (resid 2 through 24 )
15X-RAY DIFFRACTION15chain 'D' and (resid 25 through 102 )
16X-RAY DIFFRACTION16chain 'D' and (resid 103 through 119 )
17X-RAY DIFFRACTION17chain 'D' and (resid 120 through 153 )
18X-RAY DIFFRACTION18chain 'D' and (resid 154 through 168 )
19X-RAY DIFFRACTION19chain 'D' and (resid 169 through 202 )
20X-RAY DIFFRACTION20chain 'E' and (resid 2 through 93 )
21X-RAY DIFFRACTION21chain 'E' and (resid 94 through 120 )
22X-RAY DIFFRACTION22chain 'E' and (resid 121 through 241 )

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