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- PDB-6utu: Crystal structure of minor pseudopilin ternary complex of XcpVWX ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6utu | ||||||
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Title | Crystal structure of minor pseudopilin ternary complex of XcpVWX from the Type 2 secretion system of Pseudomonas aeruginosa in the P3 space group | ||||||
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![]() | PROTEIN TRANSPORT / pseudopilus / minor pseduopilin | ||||||
Function / homology | ![]() protein secretion by the type II secretion system / type II protein secretion system complex / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, Y. / Wang, S. / Jia, Z. | ||||||
Funding support | ![]()
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![]() | ![]() Title: In Situ Proteolysis Condition-Induced Crystallization of the XcpVWX Complex in Different Lattices. Authors: Zhang, Y. / Wang, S. / Jia, Z. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 345.5 KB | Display | ![]() |
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PDB format | ![]() | 219.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 251 KB | Display | ![]() |
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Full document | ![]() | 251 KB | Display | |
Data in XML | ![]() | 1.1 KB | Display | |
Data in CIF | ![]() | 14.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5vtmS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10534.801 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: xcpV, pddC, PA3099 / Production host: ![]() ![]() #2: Protein | Mass: 22247.691 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: xcpW, pddD, PA3098 / Production host: ![]() ![]() #3: Protein | Mass: 30612.576 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: xcpX, PA3097 / Production host: ![]() ![]() #4: Chemical | ChemComp-CA / #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.45 Å3/Da / Density % sol: 49.89 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion Details: 19 %-22 % PEG 2000 MME, 0.1 M Tris, pH 8.0-9.0, 0.2 M trimethylamine N-oxide (TMAO) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 X 1M / Detector: PIXEL / Date: Nov 3, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 2.83→19.91 Å / Num. obs: 42465 / % possible obs: 99.2 % / Redundancy: 10 % / Biso Wilson estimate: 79.02 Å2 / CC1/2: 0.998 / Net I/σ(I): 12.77 |
Reflection shell | Resolution: 2.83→3 Å / Num. unique obs: 6545 / CC1/2: 0.646 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5VTM Resolution: 2.85→19.91 Å / SU ML: 0.4998 / Cross valid method: FREE R-VALUE / σ(F): 1.94 / Phase error: 31.2807 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 73.29 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.85→19.91 Å
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Refine LS restraints |
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LS refinement shell |
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